The invention discloses a preparation capable of improving the cold resistance of tea trees and a preparation method for the preparation. Late spring cold greatly affects the production of tea, but an effective cold-resistant agent which ensures that the tea trees are free from freezing injury is not invented at present. The preparation mainly comprises bamboo vinegar, physiological cold-resistant substances, a growth promotion substance, an acidity regulator and the like. The preparation method comprises the following steps of: 1, taking the required bamboo vinegar in a proportion, adding a lot of trace elements which are required to be mixed, and stirring until the trace elements are dissolved; 2, dissolving physiological cold-resistant substances which are required to be mixed such as abscisic acid into absolute ethanol; 3, mixing a solution obtained in step 1 and a solution obtained in step 2, adding a proper amount of acidity regulator, regulating until the PH value is appropriate, stirring until the acidity regulator is dissolved, adding other physiological cold-resistant substances, and stirring until the physiological cold-resistant substances are dissolved; 4, dissolving a growth hormone which is required to be mixed into hot absolute ethanol, and fixing the volume by using the bamboo vinegar; and 5, mixing a solution obtained in step 3 and a solution obtained in step 4, and uniformly blending to obtain the preparation. The preparation is characterized in that a natural organic substance is taken as a main raw material, and the preparation can be safely used, does not pollute the environment, and can remarkably improve the cold resistance of the tea trees.