The invention discloses a preparation method of an orange fruit thinning agent. The method comprises: preparation of oligochitosan and preparation of a fruit thinning agent. The specific steps include: firstly subjecting chitosan and deionized water to mixed reaction, then adding hydrogen peroxide, conducting ethanol precipitation and ethanol washing to obtain oligochitosan, adding naphthylaceticacid powder, dinitrocresol, 1-aminocyclopropanehydroxyl acid, branched secondary alcohol polyoxyethylene ether, trichlorfon, naphthylacetic acid, 6-benzyladenine, and a blending agent to obtain a fruit thinning agent stock solution, and conducting dilution to obtain the orange fruit thinning agent. The invention has the beneficial effects that: after spraying the fruit thinning agent to orange branches, a layer of fruit thinning agent film can be formed on the orange branches, the film can effectively inhibit the transpiration of branches and reduce the water loss of branches, the orange fruitthinning agent has powerful blossom and fruit thinning effects, has an insecticidal function at the same time of blossom and fruit thinning, in addition, the fruit thinning agent also can increase the single fruit weight, enhance the fruit shape index, increase the flower quantity of next year, and reduce fruit rust, and also has no influence on the orange storage quality.