The invention relates to a protein fruit and vegetable cleaning agent and a preparation method thereof, belonging to the field of food processing. The protein and cerium ammonium nitrate are mixed and reacted in proportion, and the protein-cerium complex is obtained by washing and centrifuging, and the fruit and vegetable cleaning agent is prepared after compounding in proportion. The formula of the fruit and vegetable cleaning agent is mainly as follows: protein-cerium complex 10%-20%, alkyl glucoside 5%-10%, potassium citrate 5%-10%, edetate disodium 0.1%-0.5% , sodium carboxymethylcellulose 2%-4%, sodium bicarbonate 2%-5%, deionized water 51%-74%, the sum of each component is 100%. The raw material of the fruit and vegetable cleaning agent prepared by the present invention is safe and free of chemical pollution, and has the characteristics of green, degradable, simple processing, mild and non-irritating, and no secondary pollution. The problem of excessive pesticide residues.