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A lactofafe-prop pesticide residue degrading bacterium and the bacterial agent for its production

A technology of pesticide residue degradation and lactoforin, which is applied in the field of biotechnology, can solve problems such as ecological system hazards, agricultural product export earnings that affect health, and pesticide residues exceeding the standard, so as to protect the ecological environment and solve lactoferin The effects of excessive pesticide residues and low production and use costs

Active Publication Date: 2018-01-09
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system.
Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and export earnings of agricultural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Collect farmland soil where lactoferin has been applied for a long time, take 10 grams of soil sample and put it into 90ml sterile water, shake it for 20 minutes, mix the soil sample and water evenly, gradiently dilute, and spread the sample on a 1 / 10LB solid medium plate (1 gram of peptone, 0.5 gram of yeast extract, 1 gram of NaCl, 20 grams of agar powder, 1000 ml of water, pH 7.0 to 7.2), cultivated in a 30°C incubator for 48 hours, and singled out bacteria to verify the degradation effect of lactoferrin.

[0019] After expanding the cultivation of the picked single bacterium, inoculate the inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 0.5 grams of NaCl, 1000 ml of water, pH 7.0-7.2) for degradation verification, the effective single bacteria that have the degradation effect on lactofen-methyl. Through verification, an effective single strain was obtained, named Za, which was identified as Bacillus sp. T...

Embodiment 2

[0022] Degradation characteristic determination of degrading bacteria Za in inorganic salt liquid medium: Pick a single colony of Za in 3ml LB liquid medium (10 grams of peptone, 5 grams of yeast extract, 10 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), 30 ℃, 160rmp / min shaking culture for 12h, to obtain fresh bacterial liquid. Inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 0.5 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), insert 3ml of the bacterial liquid after centrifugation and suspension, cultivate on a shaking table at 30°C (160rmp / min), take samples regularly for determination, and determine lactofen content, the results are shown in Table 1, the degradation rate of lactofen-prop by the degrading bacteria Za in the inorganic salt liquid medium reached 94.8%.

[0023] Determination of the degradation characteristics of lactofen-methyl by the degrading bacteria Za in the soil: Weigh 1000g of ...

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PUM

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Abstract

The invention discloses a lactoforin pesticide residue degrading bacterium and the bacterial agent produced therefrom. The strain is a Gram-positive bacterium, which was identified as Bacillus sp., and was preserved in the China Center for Type Culture Collection on February 27, 2014. The strain preservation number is CCTCC NO: M 2014050. The culture solution after the Za strain is fermented is the bacterial agent. The Za strain can reduce the pesticide residue of lactoferin in the soil by more than 70%, and can solve the problem of exceeding the standard of the pesticide residue of lactoferrin in agricultural production.

Description

technical field [0001] The invention belongs to the high-tech field of biology, and provides a lactoforin pesticide residue-degrading bacterium and a bacterial agent produced therefrom. Background technique [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system. Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and the export of agricultural p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10
CPCB09C1/10C12N1/205C12R2001/07
Inventor 黄星张晶孙斌宋嫚成明根张浩李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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