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Medium for producing Paecilomyces cicadae spore, culture method thereof, culture product thereof and application thereof

A technology of Paecilomyces cicadae and a culturing method, which is applied in the field of culture medium for producing Paecilomyces cicadae conidia, can solve the problem of inability to obtain Paecilomyces cicadae conidia, long production cycle, low spore content of inoculants, etc. To solve the problem of excessive pesticide residues and improve renal function

Active Publication Date: 2013-12-04
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The artificial cultivation method of cicadae in existing patent is only the substratum and the method that are designed in order to obtain the fruiting body of mycelia, sporophyme bundle or encarnata body, do not have the substratum and the method specially designed in order to obtain cicadae spore powder training method
In addition, in the existing culture methods, simple liquid culture cannot obtain conidia of Paecilomyces cicadae, while solid culture has disadvantages such as long production cycle, low spore content of bacterial agent, and high cost.

Method used

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  • Medium for producing Paecilomyces cicadae spore, culture method thereof, culture product thereof and application thereof
  • Medium for producing Paecilomyces cicadae spore, culture method thereof, culture product thereof and application thereof
  • Medium for producing Paecilomyces cicadae spore, culture method thereof, culture product thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] 1. Preparation of medium:

[0050] 1. Preparation of PDA slope (solid)

[0051] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 200g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 20g of agar and 20g of sucrose, add water to 1000mL after heating and dissolving, divide into test tubes, 121°C, 1.05Kg / cm 2 , after sterilizing for 30 minutes, put it on an inclined plane, and use it after cooling.

[0052] 2. Preparation of solid phase medium

[0053] Weigh bran 800g, corn flour 150g, H 2 O 720ml, mixed cooking for 1h, sucrose 10g, KNO 3 5g, KH 2 PO 4 3g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 10g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm2 , use after 30 minutes of sterilization.

[0054] Described solid medium can also be:

[0055] Weigh 1200g of bran, 20g of silkworm chrysalis powder, 15g of sh...

Embodiment 2

[0109]1. Preparation of culture medium

[0110] 1) PDA slope preparation (solid)

[0111] With embodiment 1.

[0112] 2) Preparation of solid phase medium

[0113] Weigh bran 900g45, corn flour 600g, H 2 O1000ml, mixed cooking for 1h, sucrose 100g, KNO 3 60g, KH 2 PO 4 20g, MgSO 4 16g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 100g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0114] 3) Preparation of liquid medium

[0115] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 200g of sucrose, 10g of citric acid and heat to dissolve, add water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0116] 2. The cultivation process of Paecilomyces cicadae spores

[0117] With embodiment 1, ob...

Embodiment 3

[0119] 1. Preparation of culture medium

[0120] 1) PDA slope preparation (solid)

[0121] With embodiment 1.

[0122] 2) Preparation of solid phase medium

[0123] Weigh bran 800g, corn flour 200g, H 2 O 800ml, mix and cook for 1h, sucrose 20g, KNO 3 4g, KH 2 PO 4 2g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 20g, cooked bran and corn flour evenly, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0124] 3) Preparation of liquid medium

[0125] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 30g of sucrose, 0.3g of citric acid and heat to dissolve, make up water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0126] 2. The cultivation process of Paecilomyces cicadae spores

[0127] With embodiment 1, obtain a larg...

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Abstract

The invention belongs to the technical field of microbial fermentation, and specifically relates to media for producing Paecilomyces cicadae conidiospores, a culture method thereof, a culture product thereof and application thereof. The media for producing Paecilomyces cicadae conidiospores comprise a solid medium and a liquid medium. The culture method provided in the invention can produce considerable Paecilomyces cicadae conidiospores; the culture product of the method comprises components like polysaccharides, cordycepic acid, adenosine, triterpenoids and a plurality of amino acids, has the functions of regulating immunity, relieving fever and pain, improving renal function, lowering blood pressure, resisting radiation and tumor, reducing blood sugar, etc., and is applicable to the field of human healthy life; considerable spores are generated in the process of fermentation and the generated spores can be used as effective means of biological control, thereby solving the problem that the amount pesticide residues in agricultural products exceed the standard amount from the head stream.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a culture medium for producing conidia of Paecilomyces cicadae, a culture method, culture products and applications thereof. Background technique [0002] Cicada flower is Paecilomyces cicadae, which is an entomogenic fungus. Because it belongs to the same insect-fungal complex as Cordyceps sinensis, and contains similar chemical and nutritional components, it is often used as a substitute for Cordyceps sinensis. Cicada has various pharmacological effects such as immune regulation, antipyretic and analgesic, improvement of kidney function, sedation and hypnosis, anti-radiation, etc. It can be applied to food, health care products, medicines, cosmetics and other fields. The study found that the same nutrient components were detected in the spores of Paecilomyces cicadae as in other parts such as spore bundles and fungal plasm, and the content was even hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N3/00C12N1/14C12P1/02A23L1/30A61K36/066A61K8/99A61P35/00A61P37/02A61P3/10A61P9/12A61P17/16A61P29/00A61P13/12A01N63/04C12R1/79
Inventor 陈祝安孙长胜赵伟李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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