A strain of Providencia stutzeri jd and its application

A technology of Providencia stutzeri and Providencia spp. is applied in the field of microorganisms and can solve problems such as the inability to meet the bioremediation of pesticide residue pollution

Active Publication Date: 2021-07-06
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degradation preparations specifically targeting Sclerotinia

Method used

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  • A strain of Providencia stutzeri jd and its application
  • A strain of Providencia stutzeri jd and its application
  • A strain of Providencia stutzeri jd and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Isolation and identification of Providencia stutzeri JD

[0024] 1) Isolation and screening of Providencia stutzeri JD

[0025] Weigh 10.0g of soil polluted by sclerotia for a long time and place it in a 250mL Erlenmeyer flask, add 100mL of sterilized inorganic salt medium, add pesticide standard sample to 25mg / L, place on a shaker, and set the temperature at 30°C and 150rpm Under the condition of shaking culture 5d. Take 0.5mL supernatant from the culture solution and put it in a new inorganic salt culture solution containing 25mg / L pesticide, cultivate it under the same conditions until the culture solution becomes turbid, then take the supernatant and inoculate it in a new culture medium with 10% inoculation amount. Culture solution, repeat the above operations, and gradually increase the concentration of pesticides to 1000mg / L (pesticide concentrations are respectively 25mg / L, 50mg / L, 75mg / L, 100mg / L, 1000mg / L), to obtain enriched degrading bacteria l...

Embodiment 2

[0050] Embodiment 2: the preparation of degrading bacteria Providencia stutzeri JD preparation

[0051] 1) Preparation of liquid degrading bacterial agent

[0052] Including the following steps:

[0053] (1) Inoculate 5% bacterial suspension of strain JD in beef extract peptone salt medium, shake culture at 35° C., 150-200 rpm for 5-12 hours, and obtain strains.

[0054] (2) Inoculate the strain into a seed bottle at an inoculum amount of 1% of the volume ratio of the beef extract peptone salt medium, and vibrate at 35° C. for 5 to 12 hours at 150 to 200 rpm to obtain a seed liquid.

[0055] (3) Inoculate the obtained seed liquid into the fermentation medium (beef extract 7g / L, yeast extract 14g / L and sodium chloride 5g / L) according to the inoculum size of 10% volume ratio, at 35°C, 150~ Ferment and cultivate at 200rpm for 12 to 15 hours to obtain a fermented liquid (OD 600 greater than 20), the fermentation broth is directly diluted to be the JD liquid bacterial agent, and...

Embodiment 3

[0061] Embodiment 3: Providencia stutzeri JD is to the degradative efficiency of sclerotia net

[0062] Add sclerotium in the inorganic salt medium (with embodiment 1), make its final concentration be 50.0mg / L; Appropriate thalline is added in the inorganic salt medium containing sclerotium 50mg / L, adjust its OD600 to be 0.2 , cultured in a shaker at 30°C and 150rpm for 7 days, and samples were taken every 24 hours to determine the degradation ability of the strain. The non-inoculated inorganic salt medium added with the same concentration of pesticide was used as the control.

[0063] Apply gas chromatography to measure the residual amount of sclerotia net and calculate the degradation rate, the results are as follows: figure 2 As shown, the results show that the degrading bacteria JD can effectively degrade sclerotia in a short period of time, and the degradation rate of sclerotia with an initial concentration of 50 mg / L can reach 59.67% within 5 days, and 70.75% of sclero...

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Abstract

The present invention discloses a strain of Providencia stutzeri JD and its application. The strain has been preserved in the China Center for Type Culture Collection (CCTCC) on December 22, 2017. The address is Wuhan, China, Wuhan University, and the preservation number is for CCTCC M 2017738. The Providencia stuartii JD provided by the present invention can degrade 59.67% of the sclerotia with an initial concentration of 50 mg/L within 5 days in an inorganic salt medium, and 70.75% of the sclerotia can be degraded within 7 days. This shows that the strain can efficiently degrade Sclerotin, can effectively repair the soil and water polluted by Sclerotin, solve the problem of excessive pesticide residues and environmental pollution in the production of agricultural products, and protect the ecological environment and human health.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Providencia stuartii (Providenciastuartii) JD and its application. [0002] technical background [0003] Dimetachlone, also known as Dimetachlone, has molecular formula C 10 h 7 C l2 NO 2 , its relative molecular mass is 244.07, and its chemical name is: N-(3,5-dichlorophenyl)succinimide. The pure product of Sclerotia is white scaly crystal, and the original drug is light yellow powder. The melting point of Sclerotin is in the range of 137.5-139°C. It is easily soluble in organic solvents such as tetrahydrofuran, acetone, and dimethyl sulfoxide, soluble in methanol and ethanol, insoluble in n-hexane and petroleum ether, and almost insoluble in water. Because the content of active ingredients changes little when stored at room temperature, it is relatively stable to acid and heat, but it is relatively unstable and easy to decompose when it meets alkali and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01
CPCB09C1/10C02F3/34C02F2101/16C02F2101/306C12N1/20C12N1/205C12R2001/01
Inventor 吴小毛黄文源李荣玉张承谢颖龙友华尹显慧李明胡安龙杨再福黎晓茜刘阿丽陈媞
Owner GUIZHOU UNIV
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