Lactofen pesticide residue degradation bacterium and Lactofen pesticide residue degradation bacterium agent

A technology for the degradation of pesticide residues and lactoforin, which is applied in the field of biotechnology, can solve the problems of ecological system hazards, affecting the health of agricultural products, foreign exchange earnings from agricultural products, and pesticide residues exceeding the standard, so as to protect the ecological environment and solve the problem of lactoferin The effect of excessive pesticide residues and low production and use costs

Active Publication Date: 2015-04-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system.
Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and export earnings of agricultural products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Collect farmland soil where lactoferin has been applied for a long time, take 10 grams of soil sample and put it into 90ml sterile water, shake it for 20 minutes, mix the soil sample and water evenly, gradiently dilute, and spread the sample on a 1 / 10LB solid medium plate (1 gram of peptone, 0.5 gram of yeast extract, 1 gram of NaCl, 20 grams of agar powder, 1000 ml of water, pH 7.0 to 7.2), cultivated in a 30°C incubator for 48 hours, and singled out bacteria to verify the degradation effect of lactoferrin.

[0019] After expanding the cultivation of the picked single bacterium, inoculate the inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 0.5 grams of NaCl, 1000 ml of water, pH 7.0-7.2) for degradation verification, the effective single bacteria that have the degradation effect on lactofen-methyl. Through verification, an effective single strain was obtained, named Za, which was identified as Bacillus sp. T...

Embodiment 2

[0022] Degradation characteristic determination of degrading bacteria Za in inorganic salt liquid medium: Pick a single colony of Za in 3ml LB liquid medium (10 grams of peptone, 5 grams of yeast extract, 10 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), 30 ℃, 160rmp / min shaking culture for 12h, to obtain fresh bacterial liquid. Inorganic salt liquid medium (NH 4 NO 3 1.0 g, K 2 HPO 4 1.5 g, KH 2 PO 4 0.5 g, MgSO 4 0.2 grams, 0.5 grams of NaCl, 1000 milliliters of water, pH 7.0-7.2), insert 3ml of the bacterial liquid after centrifugation and suspension, cultivate on a shaking table at 30°C (160rmp / min), take samples regularly for determination, and determine lactofen content, the results are shown in Table 1, the degradation rate of lactofen-prop by the degrading bacteria Za in the inorganic salt liquid medium reached 94.8%.

[0023] Determination of the degradation characteristics of lactofen-methyl by the degrading bacteria Za in the soil: Weigh 1000g of ...

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PUM

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Abstract

The invention discloses a Lactofen pesticide residue degradation bacterium and a Lactofen pesticide residue degradation bacterium agent. The strain is a gram-positive bacterium which is identified as Bacillus sp. and preserved in the CCTCC on February 27, 2014 with the strain preservation number of CCTCC NO:M 2014050. A culture solution after fermentation of the strain Za is the bacterium agent. The strain Za can reduce the Lactofen pesticide residues in soil by more than 70% and can solve the problem that Lactofen pesticide residues exceed the standard in agricultural production.

Description

technical field [0001] The invention belongs to the high-tech field of biology, and provides a lactoforin pesticide residue-degrading bacterium and a bacterial agent produced therefrom. Background technique [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system. Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and the export of agricultural p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10
CPCB09C1/10C12N1/205C12R2001/07
Inventor 黄星张晶孙斌宋嫚成明根张浩李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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