64 results about "Paecilomyces cicadae" patented technology

The invention discloses a method for artificially culturing paecilomyces cicadae and application of a culturing product thereof. The method for artificially culturing paecilomyces cicadae in large scales comprises the steps of: preparing strains, dosing and packing into a box, sterilizing, inoculating, solid-fermenting, collecting and the like. The paecilomyces cicadae is cultured by utilizing grains, such as corn flour, bran, wheat, barley, rice, millet, broomcorn and the like and culture mediums of bagasse, cane sugar, shell powder, silkworm chrysalis meal and potassium nitrate. The raw materials are obtained from local resources, a large amount of production cost is saved, the culturing period is shortened, and cordyceps sobolifera obtained by culturing has the advantages of high quality and favorable stability and the like. A culture obtained by the invention can be used for preparing foods, health-care products, drugs and cosmetics with functions of fighting tumor, regulating immunity, reducing blood sugar, blood fat and blood pressure, improving eye sight, resisting radiation, dispelling heat and easing pains, calming and hypnotizing, nourishing and strengthening, improving kidney function and the like.

The invention discloses cordyceps cicadae wine and a preparation method thereof. The cordyceps cicadae wine is obtained by dipping coremium of paecilomyces cicadae and/or mycoplasma, as raw materials, in base wine, or obtained by mixing an extract of the coremium of paecilomyces cicadae and/or an extract of mycoplasma, as raw materials, with drinking wine. In the cordyceps cicadae wine, the raw materials are fully utilized, activated secondary metabolism components in the mycoplasma are effectively kept, the integrity of active components in the cordyceps cicadae wine is ensured, the product quality is stable and the production cost is low.

The invention discloses a method for improving the yield and product activity of a liquid fermentation product of Paecilomyces cicadae, which comprises the following steps: (1) extracting traditional Chinese medicinal materials composed of 10-40 parts by weight of coix seed, 10-40 parts by weight of radix astragali, 10-40 parts by weight of Chinese wolfberry and 10-40 parts by weight of rhizoma atractylodis macrocephalae through a water solution, thus obtaining a traditional Chinese medicine compound water extract liquid; (2) adding the traditional Chinese medicine compound water extract liquid prepared in the step (1), rhizoma atractylodis macrocephalae lactone and Tween-80 into a fermentation basal culture medium, thus obtaining a Paecilomyces cicadae fermentation culture medium; and (3) inoculating a Paecilomyces cicadae strain into the Paecilomyces cicadae fermentation culture medium prepared in the step (2), performing liquid fermentation to obtain a fermentation liquid, and separating to obtain a fermentation product. According to the invention, particular kinds of traditional Chinese medicine compound water extract liquid, rhizoma atractylodis macrocephalae lactone and Tween-80 are added, so that the biomass and polysaccharide yield of the Paecilomyces cicadae can be obviously improved, and meanwhile, the activity of the polysaccharide is also improved obviously.

The invention relates to a method for producing a preparation with biological bacteria, which is used for preventing plant nematodes and the application of the preparation, in particular to a paecilomyces cicadae biological bacteria preparation, a method for producing the same and the application of the same to plant nematode prevention. The paecilomyces cicadae biological bacteria preparation can be the compound of paecilomyces cicadae, culture medium of the paecilomyces cicadae, auxiliary materials and a carrier and is produced by culturing paecilomyces cicadae, fermenting the paecilomyces cicadae with liquid, propagating the paecilomyces cicadae in great qualities, directly processing the paecilomyces cicadae and absorbing the paecilomyces cicadae with the auxiliary materials and the carrier. The paecilomyces cicadae biological bacteria preparation has obvious effect on plant nematode prevention, stable performance and wide application range, reaches the standard of the quality guarantee period of biological pesticide, meets the production requirements of organic food and green food, is environmental friendly, beneficial to human health and prone to large-scale industrial production and serves as broad-spectrum biological bacteria preparation for preventing plant nematodes.

The invention relates to the production method of paecilomyces cicadae new nutritious fungus feed addictive. The strain used in the method is traditional chinese herbal medicine paecilomyces cicadae. The process includes production processes such as acquisition of strain, cultivation of slant strains and liquid seeds, liquid fermentation, cultivation of solid substrate and so on. The result of animal test indicates that the fungus feed addictive can not only improve the feed conversion rate, save cost, gain meat animals' weight more quickly, reduce the cost by way of improving animal's immunological competence and survival rate with few medicine or no medicine, but also can achieve good effect on weight gains with little quantity of addictive. The production of the fungus feed addictive has advantages of wide raw material sources, easy production, low cost, controllable quality, convenient use, wide application, good actual effect and so on.

This invention discloses a method for separating and purifying polysaccharides from Paecilomyces cicadae. The method comprises: extracting polysaccharides from Paecilomyces cicadae by water extraction and alcohol precipitation, purifying by anion exchange column chromatography and gel filtration chromatography, and freeze-drying the extract in vacuum to obtain polysaccharides from Paecilomyces cicadae. The method can retain the bioactivity of natural polysaccharides without influence on the structure, and has low requirement for apparatus and environment. Besides, the method also has such advantages as simple process, and high practicability.

The invention discloses a rapid liquid submerged fermentation method for producing paecilomyces cicadae powder. According to the method, paecilomyces cicadae strains are adopted, then a semi-continuous fermentation technology is used to rapidly produce fermentation broth containing a large amount of mycelia, and finally the fermentation broth is separated by a micro filtration membrane to obtain mycelia of paecilomyces cicadae. The conventional paecilomyces cicadae intermittent batch fermentation technology is changed, and at the same time, the mycelia are separated by a high efficient ceramic micro-filtration membrane to replace the conventional centrifugal separation. The shortages of conventional batch fermentation technology such as a lot of steps, long time period between steps, low equipment efficiency, and the like are overcome by semi-continuous fermentation technology. Moreover, a high efficient ceramic membrane is used to filter fermentation broth to reduce the mycelia that enter the filtrate, the yield of mycelia is improved, the COD of filtrate is reduced, thus the processing amount of sewage is reduced, the cells are not obstructed, and effective components in cells can be completely recovered therefore. Compared with the conventional method, the provided method can generate prominent economic benefit, social benefit, and environmental benefit.

The invention discloses protease gene obtained from paecilomyces cicadae and having a function of degrading an insect body wall, wherein the protease gene is shown in SEQ IN NO: 16; and a protease protein fragment which is obtained from the paecilomyces cicadae and has the function of degrading the insect body wall is provided, wherein the amino acid sequence of the protease is shown in SEQ IN NO: 17. By utilizing the protease gene disclosed by the invention and expressed by genes, the insect body wall can be degraded, and a new idea is provided and a new manner is created for the research on a novel, high-efficiency and safe pesticide.

The invention relates to an application of paecilomyces cicadae cordyceps polysaccharides in preparation of a chicken immunity enhancement reagent and belongs to the field of immunologic adjuvants for livestock and poultry. The paecilomyces cicadae cordyceps polysaccharides are prepared from paecilomyces cicadae sporophore or a culture medium through heat reflux extraction and later separation and purification by the aid of a ceramic membrane. The technology is simple, and immunity tests prove that the immunologic function of the chicken organisms can be remarkably improved by the immunity enhancement reagent containing the paecilomyces cicadae cordyceps polysaccharides; through matching with a new castle disease vaccine for chicken immunization, the immune response of the vaccine can be remarkably improved, the immunization lasts for a longer time, and the preparation is free of drug residues, toxic and side effects and the like, and is an environment-friendly, efficient and stable chicken immunity enhancement reagent.

The invention discloses a feed additive which is the penicillium paecilomyces cicadae (miquel) samson fermentation product. The invention further discloses a preparation method and application of the feed additive. The preparation process of the feed additive is simple, the raw materials for preparing the feed additive are easy to obtain, and the feed additive is rich in adenosine, cordycepic acid and polysaccharides. Though a little feed additive is added to the animal feed, the feed additive can achieve a significant effect. The feed additive can improve the utilization rate of the feed, improve the palatability of the animals, promote the growth and the development of the animals, improve the quality of the livestock and poultry feed and use the feed resources more reasonably. In addition, the feed additives can strength the immune regulation of the animals to improve the survival rate of the animals so that the animal feeding cost is reduced.

The invention belongs to the technical field of microbial fermentation, and specifically relates to media for producing Paecilomyces cicadae conidiospores, a culture method thereof, a culture product thereof and application thereof. The media for producing Paecilomyces cicadae conidiospores comprise a solid medium and a liquid medium. The culture method provided in the invention can produce considerable Paecilomyces cicadae conidiospores; the culture product of the method comprises components like polysaccharides, cordycepic acid, adenosine, triterpenoids and a plurality of amino acids, has the functions of regulating immunity, relieving fever and pain, improving renal function, lowering blood pressure, resisting radiation and tumor, reducing blood sugar, etc., and is applicable to the field of human healthy life; considerable spores are generated in the process of fermentation and the generated spores can be used as effective means of biological control, thereby solving the problem that the amount pesticide residues in agricultural products exceed the standard amount from the head stream.

The invention discloses a culture method of selenium-enriched cordyceps sobolifera conidial powder. Firstly, separation, purification and identification are performed on cordyceps sobolifera collected in a field to obtain Paecilomyces cicadae (Miquel.) Samson, plate cultivation is performed to obtain single colonies, then, the single colonies are transferred to a liquid medium for culture, a liquid secondary seed solution is obtained, the secondary seed solution is transferred to a composite culture medium containing organic selenium fertilizer, the temperature is controlled to 15-35 DEG C, the humidity is controlled to 60-90%, ventilation culture is performed for 4-6 weeks, and harvesting is performed to obtain selenium-enriched cordyceps sobolifera conidial powder. Artificial simulation is performed to obtain nutritional and growth conditions closest to the conditions needed by cordyceps sobolifera wild environment, growth of cordyceps sobolifera is not limited by season changes, the composite culture medium is closer to natural bamboo forest cordyceps sobolifera wild environment, the breathability is good, the water absorption and moisturizing performance are excellent, a fruiting body grows rapidly, spore head and conidial powder of the fruiting body are abundant, thick, solid, full, large in conidial powder amount, high in quality and easy to harvest, the content of selenium is 20-100 mg/Kg, the method is simple and low in cost, the spore harvesting period is short, and industrial production can be achieved.

The invention provides a fermentation method of antioxidant cordyceps sobolifera mycelia. The fermentation method comprises the following steps: respectively preparing a PDA slant culture medium, a seed culture solution and a fermentation culture medium; dividing the paecilomyces cicadae mother strain through sterile operation, and inoculating the paecilomyces cicadae mother strain to the PDA slant culture medium for activation culture to obtain an activated strain; inoculating the activated strain into a seed culture solution to obtain a primary strain; inoculating the primary strain into theseed culture solution, and culturing the primary strain according to a method for culturing the activated strain to obtain a secondary strain; inoculating the primary strain or the secondary strain into the seed culture solution for culturing to obtain a fermentation tank seed solution; inoculating the fermentation tank seed solution into the fermentation culture medium, and carrying out fermentation culture to obtain cordyceps sobolifera fermentation liquor; and discharging the cordyceps sobolifera fermentation liquor, and carrying out semi-solid fermentation culture to obtain the fermentation product of the antioxidant cordyceps sobolifera mycelia. The fermentation method is suitable for fermentation production of cordyceps sobolifera mycelia, the obtained mycelia is high in biomass andstrong in antioxidant activity, the yield of active substance polysaccharide and cordycepic acid can be remarkably increased, and the source of health-care food raw materials is increased.

The invention discloses paecilomyces cicadae and application in preparing an antibacterial agent, a probiotic preparation and an antibacterial fresh-keeping film. A novel strain-paecilomyces cicadae CH 113.1 is first separated from field entomogenous fungus cordyceps sobolifera. A strain fermentation product has an inhibition effect for conventional pathogenic microorganisms, the inhibition rate for the escherichia coli can reach 70 percent or more, and the biological bacteriostatic agent can be applied to the fresh keeping of food. The strain has a good application prospect in the field of food.

The invention provides a pecilomyces cicadae fermentation culture medium and a method for preparing ergosterol by the paecilomyces cicadae fermentation, wherein raw material components of the paecilomyces cicadae fermentation culture medium comprise bamboo shoot heads, potassium dihydrogen phosphate, ferrous sulfate and magnesium sulfate. The paecilomyces cicadae fermentation culture medium can beused as a fermentation culture medium for preparing ergosterol by paecilomyces cicadae; meanwhile, the paecilomyces cicadae fermentation culture medium takes the bamboo shoot heads as main raw materials, can reasonably utilize the bamboo shoot head resources, solves the problem of resource waste and environmental pollution caused by the fact that most of bamboo shoot head residues after eating orprocessing are discarded randomly or simply buried, has potential economic value and wide market prospect.

The invention belongs to the field of technologies for making fruit vinegar, and provides a method for producing hovenia acerba fruit vinegar.The method includes steps of pulping raw materials; sterilizing the raw materials; fermenting paecilomyces cicadae; fermenting alcohol; fermenting acetic acid; spraying and filtering vinegar; canning and sterilizing the vinegar.Compared with existing methods for making table vinegar, the method for producing the hovenia acerba fruit vinegar has the advantages that the alcohol fermentation liquor yield can be increased by 10-15% as compared with the liquor yields of the traditional methods for making the table vinegar, and the hovenia acerba fruit vinegar yield can be increased by 10-15%; chemical preservatives for the hovenia acerba fruit vinegar can be omitted owing to antibacterial oligopeptide of certain antibacterial components produced in paecilomyces cicadae fermentation procedures by means of metabolism, and the hovenia acerba fruit vinegar has good flavor and tastes delicious.

The invention provides an efficient cultivation method of isaria cicadae miq, which comprises the following steps of: 1) selecting an olive tree orchard with sandy soil, intercropping gynostemma pentaphyllum under olive trees, soaking the egg branches of the isaria cicadae miq in weak acid electrolyzed water and airing under the sun, then transferring the egg branches of the isaria cicadae miq toa heat preservation room, controlling the humidity of the heat preservation room to be 70-8% and the temperature to be 30-35 DEG C, spraying the egg branches once every day by using balsam pear juiceat 6.00 p. m. until golden cicada is hatched and put into the orchard for cultivation; 2) carrying out weeding and insect prevention and topdressing on the gynostemma pentaphylla during the cultivation period of the golden cicada; 3) putting the golden cicada into a cultivation chamber after killing the golden cicada, controlling the oxygen partial pressure, temperature and humidity in the cultivation chamber, and continuously cultivating to obtain the isaria cicadae miq. The method has the advantages of high hatching rate of the golden cicada eggs, and promotion of the transformation of paecilomyces cicadae from a nutrient stage to a sexual stage and the like.

The invention discloses astragalus membranaceus-paecilomyces cicadae fermented fungal substance and a preparation method and application thereof. The fermented fungal substance is prepared by liquid fermentation by taking an enzymatic hydrolysate of astragalus membranaceus as a fermentation substrate and paecilomyces cicadae as a fermentation strain; wherein the enzymatic hydrolysate of astragalusmembranaceus is obtained by enzymatic hydrolysis of astragalus membranaceus through cellulase and pectinase. The astragalus membranaceus-paecilomyces cicadae fermented fungal substance has significantly increased astragalus polysaccharide content, and has significant effects in treating hyperuricemia and / or hypertriglyceridemia.

The invention discloses a radix astragali-penicillium cicada fermentation bacterium and application thereof. The fermentation bacterium is prepared from medicinal radix astragali powder as a fermentation substrate and penicillium cicada as a fermentation bacterium through solid fermentation. The radix astragali-penicillium cicada fermentation bacterium disclosed by the invention has a remarkable function of treating hyperuricemia and hypertriglyceridemia.

The invention discloses a cultivating method for imitative wild Cordyceps cicadae, comprising the following steps of: 1) an olive tree orchard with sandy soil was selected, gynostemma pentaphyllum isintercropped under the olive trees, small branches with cicada eggs are soaked in weak acid electrolyzed water and dried in the sun, and the cicadas are incubated for breeding; 2) weeding, preventinginsects and top-dressing are conducted for gynostemma pentaphyllum on a regular basis during the breeding period of the cicadas; 1) by laying a layer of sandy soil with a humidity of 40-50% in the culture room, the artificially collected cicadas are killed with ozone and dried till the humidity is 60-80% of the initial stage, then the dried cicadas are sprayed and cultured to Paecilomyces cicadaeof the exponential growth phase and transferred to the culture room to bury for 4-6 cm deep in sandy soil. By controlling the oxygen partial pressure, temperature and humidity in the culture room, thecicadas are continuously cultured till the Cordyceps cicadae is obtained. The method has the characteristics of high hatching rate of cicada eggs and promoting the transformation of Paecilomyces cicadae from vegetative stage to sexual stage.

The invention relates to the technical field of feed additives, in particular to a special micro-ecological preparation for laying hens and a preparation method of the special micro-ecological preparation. The special micro-ecological preparation for the laying hens comprises the following raw materials: bacillus natto powder, aspergillus niger powder, calcium tartrate and chitosan oligosaccharide, wherein the bacillus natto powder and the aspergillus niger powder are prepared by adopting a solid fermentation method, the calcium tartrate is prepared by decalcifying shrimp and crab shells, andthe chitosan oligosaccharide is prepared by taking the decalcified shrimp and crab shells as a raw material to prepare a fermentation culture medium, inoculating bacillus subtilis and paecilomyces cicadae, and carrying out fermentation degradation to obtain the special micro-ecological preparation. Laying hen feed added with the special micro-ecological preparation for the laying hens is used forfeeding the laying hens, so that the concentrated protein content of obtained eggs can be increased, meanwhile, the eggshell thickness is increased, and the quality of the eggs is remarkably improved.

The invention discloses a medicinal liquor with efficacies of tonifying the liver and kidney. The medicinal liquor is characterized by comprising the following ingredients: 15 grams of fructus psoraleae, 3 grams of Ligusticum wallichii, 5 grams of Paecilomyces cicadae, 10 grams of chrysanthemum, 6 grams of fruit of Chinese wolfberry, 5 grams of white quartz, 3 grams of Amomum cardamomum, 15 grams of cinnamon, 3 grams of Poria cocos and poria with hostwood, 10 grams of fructus viticis, 10 grams of honey and 800 ml of liquor with alcoholic strength of 40%. The composition of the formula of the invention can play a synergistic effect, and can specifically play the effects of invigorating the kidney, replenishing essence, nourishing blood, activating meridians, and is applicable to the treatment of fatigue caused by insufficient essence and blood; and the medicinal liquor has the advanategs of simple production, obvious effect, low cost and benefit for the body health of people.

The invention belongs to the technical field of traditional Chinese medicine preparations and particularly relates to a house lizard/Paecilomyces cicadae bidirectional solid ferment and further discloses a preparation method thereof and application of the house lizard/Paecilomyces cicadae bidirectional solid ferment in the preparation of liver cancer drugs. The house lizard/Paecilomyces cicadae bidirectional solid ferment is made by further fermenting a Paecilomyces cicadae liquid based on existing house lizard medicinal material; with unique properties and functional components of the house lizard and Paecilomyces cicadae, the antitumor function of the house lizard medicinal material is improved through deep interaction of the house lizard and Paecilomyces cicadae. The effective components of the house lizard/Paecilomyces cicadae bidirectional solid ferment are acquired by water extraction; the water extract can inhibit HepG2 liver cancer cells significantly and is suitable for the preparation of liver cancer drugs.

The present invention discloses yaochi camellia nitidissima. A raw material formula of the yaochi camellia nitidissima comprises the following raw materials in mass ratios: 0.5g-1g of camellia nitidissima, 0.5g-1g of fructus momordicae, 0.5g-1g of rhizoma alismatis, 0.5g-1g of cornus officinalis, 0.5g-1g of longan pulp, 0.5g-1g of caulis spatholobi, 0.5g-1g of paecilomyces cicadae, 0.5g-1g of cordyceps sinensis,3.5g-4g of Chinese wolfberry fruits, 1.5g-2g of tea stems, 1.5g-2g of momordica grosvenori flowers, 1.5g-2g of radix puerariae, 1.5g-2g of Chinese yams, 1.5g-2g of poria cocos and 1.5g-2g of semen euryales. The prepared yaochi camellia nitidissima has remarkable effects on treating various diseases such as hyperlipidemia, hypertension, hypotension, diabetes, stomach illness, kidneydeficiency, arthritis, urogenital inflammation, kidney and urinary calculus, constipation, etc., the various raw materials are mixed, stirred and crushed into the yaochi camellia nitidissima, medicinal properties of the camellia nitidissima can be improved, manufacture processes are simple, the manufacturing cost is reduced, and production efficiency is improved.