Bacillus cereus LGY06 for efficiently degrading napropamide and application and use method of bacillus cereus LGY06

A technology of Bacillus cereus and napropamide, which is applied in the field of microorganisms, can solve problems such as the inability to meet the bioremediation of pesticide residue pollution, and achieve the effects of protecting the ecological environment and human health, good removal effect and low production cost.

Active Publication Date: 2015-11-04
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degradation preparations specifically targeting napropamide

Method used

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  • Bacillus cereus LGY06 for efficiently degrading napropamide and application and use method of bacillus cereus LGY06
  • Bacillus cereus LGY06 for efficiently degrading napropamide and application and use method of bacillus cereus LGY06
  • Bacillus cereus LGY06 for efficiently degrading napropamide and application and use method of bacillus cereus LGY06

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Effect test

Embodiment 1

[0017] Embodiment 1 of the present invention: Isolation and identification of degrading bacteria LGY06

[0018] 1) Isolation and screening of degrading bacteria LGY06

[0019] Weigh 10.0 g of long-term napropamide-contaminated soil into a 250 mL conical flask, add 100 mL of inorganic salt medium, add napropamide to a concentration of 100 mg / L, and place the conical flask on a shaker (28°C , cultured at 150 rpm for 7 days, took 5 mL of the culture solution and inoculated it into fresh basal medium (herbicide 100 mg / L), and then cultured it in a shaker flask at 28°C for 7 days, and so on for 5 times of inoculation, according to napropamide The concentration gradient of 100 mg / L was increased, so that the concentration of napropamide in the final enriched culture solution reached 500 mg / L, and the enriched degradation strain was obtained.

[0020] During the enrichment and separation of degrading bacteria, after every 2 transfers, the bacterial suspension was prepared into 10 -...

Embodiment 2

[0039] Embodiment 2 of the present invention: preparation of degraded napropamide preparation:

[0040] Including the following steps:

[0041] 1) Cultivate the strain of Bacillus cereus LGY06 in an inorganic salt medium at 30-35°C and 150-200 rpm to the logarithmic growth phase to obtain strains;

[0042] 2) Inoculate the above-mentioned strains into the seed bottle with an inoculum amount of 10% of the volume ratio of the inorganic salt medium, and vibrate at 30-35°C and 150-200 rpm until the logarithmic growth phase to obtain the seed liquid;

[0043] 3) Inoculate the obtained seed liquid into the fermentation medium according to the inoculation amount of 10% by volume, and ferment and cultivate it at 30-35°C and 150-200 rpm for 45-50h to obtain a fermentation liquid, the number of bacteria in the fermentation liquid is greater than 3.0×10 9 cfu / mL, the fermentation broth was directly diluted into a liquid bacterial agent, and the number of bacteria in the liquid bacteri...

Embodiment 3

[0044] Embodiment 3 of the present invention: degradation efficiency of degrading bacteria LGY06 to napropamide

[0045] Napropamide was added to the inorganic salt medium (same as Example 1) to make the final concentration 50.0 mg / L; the inoculation concentration was 2.54×10 8 cfu / mL bacterial agent, and set the culture medium without inoculation as the control, culture in the dark at 30°C (150 rpm) for 0~7d, and take samples regularly. Apply the HPLC method to measure the residual amount of napropamide and calculate the degradation rate, the results are as follows: figure 2 As shown, the results show that the degrading bacteria LGY06 can effectively degrade azoxystrobin in a short period of time, the degradation rate of 50.0 mg / L napropamide within 5 days reached 66.39%, and the degradation rate of 50.0 mg / L napropamide within 7 days The degradation rate reached 76.81%, respectively, which has a good biodegradation effect. In the control without bacteria, the degradation...

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Abstract

The invention discloses a bacillus cereus LGY06 for efficiently degrading napropamide and a use method and application of the bacillus cereus LGY06. A strain is preserved in China Center for Type Culture Collection (CCTCC) in June 19, 2015, and the preservation number is CCTCC NO:M 2015398. The rate for degrading napropamide with density of 50.0 mg/L reaches 76.81% when the bacillus cereus LGY06 is placed in a salt medium for 7 days, it shows that the strain can efficiently degrade napropamide and effectively restore soil and water body polluted by napropamide, the problems that the content of pesticide residues exceeds the standard and the environment is polluted during production of agricultural products are solved, and the ecological environment and human health are protected.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacillus cereus LGY06 capable of efficiently degrading napropamide and its application and use method. Background technique [0002] The chemical name of Napropamide is N,N-diethyl-2-(α-naphthyloxy)propionamide, also known as napropamide and Dahuili. The basic physical and chemical properties are shown in Formula 1. Napropamide belongs to arylamine herbicides, which can be absorbed by weed roots or bud sheaths, inhibit the synthesis of certain enzymes in the body, and cause root buds to fail to grow and die. Napropamide is a selective pre-emergence soil treatment agent with a wide herbicidal spectrum, which can control annual grass weeds and some broad-leaved weeds and sedge weeds. It is suitable for a variety of crops and is selective for many crops. upland herbicides. [0003] After napropamide enters the field soil, its half-life is as long as two months. One applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/085C02F101/38
Inventor 吴小毛李明龙友华尹显慧李荣玉张承
Owner GUIZHOU UNIV
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