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Nicosulfuron pesticide residue degrading bacterium and inocula produced therefrom

A technology for the degradation of pesticide residues and nicosulfuron, which is applied in the field of biotechnology, can solve the problems of ecological system hazards, affecting the health of agricultural products, export of agricultural products, and pesticide residues exceeding the standard, so as to protect the ecological environment and solve the problem of nicosulfuron pesticide residues The effect of exceeding the standard and low production and use costs

Inactive Publication Date: 2010-12-08
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecological system.
Extensive use of pesticides has caused pesticide residues in agricultural products to exceed the standard, seriously affecting people's health and export earnings of agricultural products

Method used

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Embodiment Construction

[0013] The invention provides a degrading bacterial agent for eliminating nicosulfuron pesticide residues. The bacterial strain used is Gram-negative bacteria YM3, which is isolated from farmland soil where nicosulfuron pesticide has been used for a long time in Tai'an City, Shandong Province. On December 3, 2008, it was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee. The culture preservation number is: CGMCC No.2781, and it was identified as Pseudomonas sp. The main biological characteristic is G - , the bacteria are rod-shaped, the size is 0.5-1.0×1.5-5.0μm, catalase, nitrate reduction are positive; oxidase, V.P. reaction, methyl red, indole reaction are negative; can not hydrolyze starch and gelatin, can not Use citrate.

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Abstract

The invention provides a decomposing microbial inoculum that is used for eliminating nicosulfuron pesticide residue and belongs to the advanced technical field of biology. A Gram-negative bacteria strain YM3 identified as Pseudomonas sp. is used. The strain has following main biological characteristics: G<->, bacilliform thallus with size of (0.5-1.0) multiplied by (1.5-5.0) num, and positive in reduction reaction of catalase and nitrate; negative in oxidase and V.P. reaction and methyl red and indole reaction; non-hydrolysis of starch and isinglass, and non-utilization of citrate. A culture fluid that is obtained after fermentation of the YM3 strain is the microbial inoculum. By applying the product of the decomposing microbial inoculum, the residue amount of the nicosulfuron pesticide in soil can be lowered by over 72 percent, thus solving the problem of overproof nicosulfuron pesticide residue in agricultural industry.

Description

1. Technical field [0001] The invention provides a nicosulfuron pesticide residue-degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production of green and pollution-free agricultural products in modern agricultural production. 2. Background technology [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by plant diseases, insects, and weed damage for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production. played an invaluable role in the modernization process. But at the same time, pesticides are some toxic and harmful chemical substances. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also cause serious harm to the ecolo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10A62D101/28A62D101/04C12R1/38
Inventor 李顺鹏马吉平黄星王哲孙纪全
Owner NANJING AGRICULTURAL UNIVERSITY
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