Stenotrophomonas maltophilia XQ08 efficiently degrading deltamethrin and application thereof

A technology of Stenotrophomonas and deltamethrin, which is applied in the field of microorganisms, can solve the problems such as bioremediation of pesticide residue pollution that cannot be satisfied, and solve the problem of excessive pesticide residues and environmental pollution, with good removal effect and protection Effects on ecological environment and human health

Inactive Publication Date: 2016-05-25
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the diversity of pesticide structures, microbial degradation of pesticides is often highly targeted, resulting in the existing resource pool of degrading bacteria far from meeting the actual needs of bioremediation of pesticide residue pollution.
At present, there are no degrading microorganisms and related degradation preparations specifically targeting deltamethrin

Method used

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  • Stenotrophomonas maltophilia XQ08 efficiently degrading deltamethrin and application thereof
  • Stenotrophomonas maltophilia XQ08 efficiently degrading deltamethrin and application thereof
  • Stenotrophomonas maltophilia XQ08 efficiently degrading deltamethrin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 of the present invention: isolation and identification of degrading bacteria XQ08,

[0021] 1) Isolation and screening of degrading bacteria XQ08,

[0022] Weigh 10.0 g of deltamethrin-contaminated soil for a long time into a 250 mL conical flask, add 100 mL of inorganic salt medium, add deltamethrin to a concentration of 100 mg / L, and place the conical flask on a shaker (28°C , culture at 150rpm for 7 days, inoculate 5mL culture solution into fresh basal medium (herbicide 100mg / L), then culture in shake flask at 28°C for 7 days, and so on for 5 times of inoculation, according to the dosage of deltamethrin 100mg / L The concentration gradient increases, so that the concentration of deltamethrin in the final enrichment culture solution reaches 500 mg / L, and the enrichment and degradation bacterial strain is obtained.

[0023] During the enrichment and separation of degrading bacteria, after every 2 transfers, the bacterial suspension was prepared into 10 -2 ...

Embodiment 2

[0042] Embodiment 2 of the present invention: preparation of degraded deltamethrin preparation,

[0043] Including the following steps:

[0044] 1) Stenotrophomonas maltophilia XQ08 strain was cultured in beef extract peptone salt medium at 30-35°C and 150-200rpm to logarithmic growth phase to obtain strains;

[0045] 2) Inoculate the above-mentioned bacteria into the seed bottle according to the inoculum amount of 10% of the volume ratio of the beef extract peptone salt medium, and cultivate it to the logarithmic growth phase at 30-35 ° C and 150-200 rpm to obtain the seed liquid;

[0046] 3) Inoculate the obtained seed liquid into the fermentation medium according to the inoculation amount of 10% volume ratio, ferment and cultivate at 30-35°C and 150-200rpm for 45-50h, obtain the fermentation liquid, and detect the fermentation with a UV spectrophotometer OD of liquid 600 If it is greater than 1.5, the fermentation broth is directly diluted into a liquid bacterial agent, a...

Embodiment 3

[0047] Embodiment 3 of the present invention: degrading bacteria QX08 is to the degradation performance of deltamethrin,

[0048] Add deltamethrin to the inorganic salt medium (same as Example 1) to make the final concentration 50.0mg / L; add the microbial agent of the degradation bacteria QX08 to make it OD 600 0.6, and the culture medium without inoculation was used as the control, cultured in the dark at 30°C (150rpm) for 0~5d, and samples were taken regularly. Apply the HPLC method to measure the residual amount of deltamethrin and calculate the degradation rate, the result is as follows figure 2 As shown, the results show that the degrading bacteria QX08 can effectively degrade deltamethrin in a short period of time, and the degradation rate of 50.0mg / L deltamethrin within 5 days reaches 67.04%, which has a good biodegradation effect. The test results showed that the degrading bacteria QX08 had good degrading ability to 50.0mg / L deltamethrin. This result shows that the ...

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Abstract

The invention discloses stenotrophomonas maltophilia XQ08 efficiently degrading deltamethrin and application thereof. The strain stenotrophomonas maltophilia XQ08 is preserved in China Center for Type Culture Collection (CCTCC) on December 8th, 2015 at the address of Wuhan University in Wuhan, China and with the preservation number of CCTCC M 2015726. The Bacillus cereus stenotrophomonas maltophilia XQ08 provided by the invention has the advantage that the degradation rate on deltamethrin (50.0mg/L) in an inorganic salt culture medium after 5 days reaches 67.04%, so that the strain can efficiently degrade deltamethrin and can effectively restore deltamethrin-polluted soil and water, the excessive pesticide residue problem in production of agricultural products and environmental pollution problem are solved and ecological environment and human health are protected.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Stenotrophomonas maltophilia XQ08 for efficiently degrading deltamethrin and its application. Background technique [0002] Deltamethrin, the chemical name is α-cyanophenoxy (1R,3R)-3-(2,2-dibromovinyl)-2,2-dimethylcyclopropanecarboxylate, Also known as "Disha" and "Kaisuling", it is one of the fastest-growing insecticides among the pyrethroid insecticides, and it is also the one with the highest toxicity. Deltamethrin has contact and stomach poisoning effects, the contact effect is rapid, the knockdown is strong, and there is no fumigation and systemic effect. Deltamethrin has a long-lasting effect (7~12 days) and a wide insecticidal spectrum. It is effective against various pests such as Lepidoptera, Orthoptera, Thysanoptera, Hemiptera, Diptera, and Coleoptera. [0003] Due to its broad-spectrum and high-efficiency characteristics, deltamethrin is widely used in agricultural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 吴小毛侯少锋李荣玉张承龙友华尹显慧
Owner GUIZHOU UNIV
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