A kind of application of the fermentation product of Paecilomyces cicadae
A technology of Paecilomyces cicadae and fermentation products, which is applied in the field of Paecilomyces cicadae fermentation products, can solve the problems of long production cycle, inability to obtain Paecilomyces cicadae conidia, and high cost, and achieve the effect of improving kidney function
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Embodiment 1
[0036] 1. Preparation of medium:
[0037] 1. PDA slope preparation (solid)
[0038] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 200g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 20g of agar and 20g of sucrose, add water to 1000mL after heating and dissolving, divide into test tubes, 121°C, 1.05Kg / cm 2 , after sterilizing for 30 minutes, put it on an inclined plane, and use it after cooling.
[0039] 2. Preparation of solid phase medium
[0040] Weigh bran 800g, corn flour 150g, H 2 O720ml, mix and cook for 1h, sucrose 10g, KNO 3 5g, KH 2 PO 4 3g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 10g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.
[0041] Described solid medium can also be:
[0042] Weigh 1200g of bran, 20g of silkworm chrysalis powder, 15g of shell p...
Embodiment 2
[0096] 1. Preparation of culture medium
[0097] 1) PDA slope preparation (solid)
[0098] With embodiment 1.
[0099] 2) Preparation of solid phase medium
[0100] Weigh bran 900g45, corn flour 600g, H 2 O1000ml, mixed cooking for 1h, sucrose 100g, KNO 3 60g, KH 2 PO 4 20g, MgSO 4 16g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 100g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.
[0101] 3) Preparation of liquid medium
[0102] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 200g of sucrose, 10g of citric acid and heat to dissolve, add water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.
[0103] 2. The cultivation process of Paecilomyces cicadae spores
[0104] With embodiment 1, obta...
Embodiment 3
[0106] 1. Preparation of culture medium
[0107] 1) PDA slope preparation (solid)
[0108] With embodiment 1.
[0109] 2) Preparation of solid phase medium
[0110] Weigh bran 800g, corn flour 200g, H 2 O800ml, mixed cooking for 1h, 20g of sucrose, KNO 3 4g, KH 2 PO 4 2g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 20g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.
[0111] 3) Preparation of liquid medium
[0112] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 30g of sucrose, 0.3g of citric acid and heat to dissolve, make up water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.
[0113] 2. The cultivation process of Paecilomyces cicadae spores
[0114] With embodiment 1, obtain ...
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