A kind of application of the fermentation product of Paecilomyces cicadae

A technology of Paecilomyces cicadae and fermentation products, which is applied in the field of Paecilomyces cicadae fermentation products, can solve the problems of long production cycle, inability to obtain Paecilomyces cicadae conidia, and high cost, and achieve the effect of improving kidney function

Active Publication Date: 2015-12-23
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The artificial cultivation method of cicadae in existing patent is only the substratum and the method that are designed in order to obtain the fruiting body of mycelia, sporophyme bundle or encarnata body, do not have the substratum and the method specially designed in order to obtain cicadae spore powder training method
In addition, in the existing culture methods, simple liquid culture cannot obtain conidia of Paecilomyces cicadae, while solid culture has disadvantages such as long production cycle, low spore content of bacterial agent, and high cost.

Method used

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  • A kind of application of the fermentation product of Paecilomyces cicadae
  • A kind of application of the fermentation product of Paecilomyces cicadae
  • A kind of application of the fermentation product of Paecilomyces cicadae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of medium:

[0037] 1. PDA slope preparation (solid)

[0038] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 200g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 20g of agar and 20g of sucrose, add water to 1000mL after heating and dissolving, divide into test tubes, 121°C, 1.05Kg / cm 2 , after sterilizing for 30 minutes, put it on an inclined plane, and use it after cooling.

[0039] 2. Preparation of solid phase medium

[0040] Weigh bran 800g, corn flour 150g, H 2 O720ml, mix and cook for 1h, sucrose 10g, KNO 3 5g, KH 2 PO 4 3g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 10g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0041] Described solid medium can also be:

[0042] Weigh 1200g of bran, 20g of silkworm chrysalis powder, 15g of shell p...

Embodiment 2

[0096] 1. Preparation of culture medium

[0097] 1) PDA slope preparation (solid)

[0098] With embodiment 1.

[0099] 2) Preparation of solid phase medium

[0100] Weigh bran 900g45, corn flour 600g, H 2 O1000ml, mixed cooking for 1h, sucrose 100g, KNO 3 60g, KH 2 PO 4 20g, MgSO 4 16g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 100g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0101] 3) Preparation of liquid medium

[0102] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 200g of sucrose, 10g of citric acid and heat to dissolve, add water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0103] 2. The cultivation process of Paecilomyces cicadae spores

[0104] With embodiment 1, obta...

Embodiment 3

[0106] 1. Preparation of culture medium

[0107] 1) PDA slope preparation (solid)

[0108] With embodiment 1.

[0109] 2) Preparation of solid phase medium

[0110] Weigh bran 800g, corn flour 200g, H 2 O800ml, mixed cooking for 1h, 20g of sucrose, KNO 3 4g, KH 2 PO 4 2g, MgSO 4 2g, with a small amount of H 2 After O is dissolved, mix with silkworm chrysalis powder 20g, and cooked bran and corn flour evenly, at 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0111] 3) Preparation of liquid medium

[0112] Pick fresh, non-germinated and disease-free potatoes, peel and wash them, cut into small slices, weigh 300g, add 1000mL of water, boil for 30min and filter, the filtrate is potato juice. Then add 30g of sucrose, 0.3g of citric acid and heat to dissolve, make up water to 1000mL, after mixing, 121°C, 1.05Kg / cm 2 , use after 30 minutes of sterilization.

[0113] 2. The cultivation process of Paecilomyces cicadae spores

[0114] With embodiment 1, obtain ...

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PUM

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Abstract

The invention belongs to the technical field of fermentation of microorganism, and particularly relates to application of a fermentation product of paecilomyces cicadae in the field of a functional food, the field of drug preparation and the field of preparation of daily chemical products. The fermentation product disclosed by the invention contains the components such as polysaccharide, cordycepic acid, adenosine, triterpenes, various amino acids and the like, has the functions of adjusting immunity, relieving fever and easing pain, improving renal function, reducing blood pressure, resisting radiation and tumors, reducing blood sugar and the like, and can be applied to the field of healthy life of people.

Description

[0001] The present invention is a divisional application. [0002] Original application information: [0003] Application date: 2011-5-12 [0004] Application number: 201110122002.4 [0005] Invention title: a medium for producing Paecilomyces cicadae spores, a culture method, a culture product and its application technical field [0006] The invention belongs to the technical field of microbial fermentation, and in particular relates to the use of a fermentation product of Paecilomyces cicadae. Background technique [0007] Cicada is Paecilomycescicadae, which is an entomogenous fungus. Because it belongs to the same insect-bacteria complex as Cordyceps sinensis, and contains similar chemical and nutritional components, it is often used as a substitute for Cordyceps sinensis. Cicada has various pharmacological effects such as immune regulation, antipyretic and analgesic, improvement of kidney function, sedation and hypnosis, anti-radiation, etc. It can be applied to food...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/30A61K36/066A61K8/99A61P37/02
Inventor 陈祝安孙长胜赵伟李成
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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