Degradating bacteria for hexachlorocyclohexane pesticide residue and its produced strain

A technology of pesticide residue degradation and 666 is applied in the field of 666 pesticide residue degrading bacteria and the inoculum produced by them, which can solve the problems of relatively mature technology, long residual time and difficult decomposition, etc., and achieve protection The effect of good health, good removal effect and low production cost

Inactive Publication Date: 2006-09-27
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

666 is not easy to decompose, insoluble in water, heat-resistant and acid-resistant, soluble in fat and organic solvents, so the residual time on the ground, water and objects is long
However, there has been no relatively mature technology for the comprehensive treatment of the environment where 666 has been used.

Method used

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  • Degradating bacteria for hexachlorocyclohexane pesticide residue and its produced strain
  • Degradating bacteria for hexachlorocyclohexane pesticide residue and its produced strain
  • Degradating bacteria for hexachlorocyclohexane pesticide residue and its produced strain

Examples

Experimental program
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Embodiment Construction

[0024] 1 Isolation and identification of strains

[0025] Take 10g of contaminated soil, put it in 100ml of sterile water, and shake it for 5 minutes to get the soil bacteria suspension. Take 5ml of the bacterial suspension, add it to 100ml of inorganic salt medium, add HCH as the only carbon source, and culture it on a shaker at 30°C and 180rpm to obtain the enrichment solution, serially dilute and coat the LB plate with HCH added, so we get A bacterium that can grow on the medium and degrade HCH (see figure 1 ), named BHC-A, which was further purified and identified as Sphingomonas sp. after multiple transfers. Its main biological characteristics are: facultative aerobic, polar flagella, G-, Straight or Campylobacter, catalase and oxidase activity, V.P. reaction is positive, methyl red reaction is negative, yellow pigment , Capsules are produced around the bacteria, which can use glucose, glycine and fructose to oxidize and produce acid. The BHC-A strain can grow on hexa-...

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Abstract

The present invention provides a kind of bacteria preparation for efficiently degrading hexachlorocyclohexane (HCH) pesticide residue. The strain BHC-A is Sphingomonas sp., can growth with HCH as the unique carbon source and energy source, and degrade alpha-HCH, beta-HCH, gamma-HCH and delta-HCH in short time at high efficiency. The present invention solves the difficult problem of aerobically biodegrading beta-HCH, and can mineralize 5 mg / L beta-HCH completely within 12 hr. Applying the degrading bacteria product directly can lower the residual HCH amount in soil by over 95 % to lower the HCH residue in agricultural product.

Description

1. Technical field [0001] The present invention is a HCH pesticide residue degrading bacterium and the bacterial agent produced therefrom, belonging to the high-tech field of biology, which degrades chemical pesticide residues by means of microorganisms, and is suitable for environmental pollution control and green and non-toxic in modern agricultural production. Production and processing of polluted agricultural products. 2. Technical background [0002] 666 is a kind of organochlorine pesticide. It is an organic synthetic insecticide that appeared in the mid-1940s. Because of its low price and broad spectrum, it is effective against pests such as agriculture, forestry, animal husbandry, wool spinning, grain storage, and hygiene. safe and widely used. Since it was discovered in 1962 that it has residual poison and pollutes the environment, since 1970, most countries have declared it banned, and my country has stopped production since April 1, 1983. After the 1990s, the us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 李顺鹏马爱芝武俊张国顺洪青何健管晓进
Owner NANJING AGRICULTURAL UNIVERSITY
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