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Carbofuran pesticide residual degraded bacteria and its produced bacterial agent

A pesticide residue degradation and carbofuran technology, which is applied in the field of high biotechnology, can solve the problems of low production and use costs, achieve low production costs, solve the problem of excessive pesticide carbofuran residues, and have good removal effects

Inactive Publication Date: 2004-12-22
南京农大环境生物工程有限公司
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Problems solved by technology

At the same time, the production and use costs are lower

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  • Carbofuran pesticide residual degraded bacteria and its produced bacterial agent
  • Carbofuran pesticide residual degraded bacteria and its produced bacterial agent
  • Carbofuran pesticide residual degraded bacteria and its produced bacterial agent

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Embodiment Construction

[0024] 1 Isolation and identification of strains

[0025] Take the activated sludge particles domesticated in the laboratory, dissolve them in sterile water, and perform gradient dilution. The diluted solution is coated on 100mg / L carbofuran inorganic salt ion solid medium, and cultured at 30°C for several days. Pick a single colony from it (see figure 1 ), identified as Sphingomonas agrestis. The main biological characteristic is G - , The bacteria are long rod-shaped, slightly curly, no spores, aerobic growth, catalase positive; can oxidize pentose, hexose and disaccharide to produce acid, but do not decompose polyhydric alcohol and inulin. It can grow with carbofuran as the only carbon source and nitrogen source, and completely mineralize it into CO 2 And water and simple inorganic compounds. The degradation rate of carbofuran is more than 95% under the condition of bottle in the laboratory. The bacterium can be produced with general fermentation equipment in the ferm...

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Abstract

A bacterium, Sphingomonas agrestis which shows negative to Gram reaction, and its preparation for degradating the residual carbofuran are disclosed. Said sphingomonas agrestis features that the carbofuran is the only carbon source and nitrogen source needed by its growth and can be mineralized into CO2, water and simple inorganic compound. Its preparation is prepared through slant culture, shake flask culture and culturing in seeding tank.

Description

1. Technical field [0001] The invention relates to a carbofuran pesticide residue degrading bacterium and a bacterial agent produced therefrom, which belong to the high-tech field of biology, use microorganisms to degrade chemical pesticide residues, and are suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Technical background [0002] Since the use of organic pesticides such as DDT and HCH, chemical pesticides have been paid more and more attention by people. With the development of agricultural production, the problems of plant diseases, insect pests and weeds that harm the growth of crops need to be solved urgently, which also promotes the continuous increase in the variety and quantity of pesticide production. At present, the total content of foreign pesticides is about 2 million tons based on 100% active ingredients. There are more than 1,300 kinds of pesticides produced. There are about...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00
Inventor 李顺鹏武俊何健崔中利
Owner 南京农大环境生物工程有限公司
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