Method for preparing methyl parathion degradation bacterium and enzyme preparation thereof

Abstract

The invention relates to methyl parathion degradation fungus and the preparing method of the enzyme preparation thereof, and belongs to the biological high technical field. The methyl parathion degradation fungus strain is plesiomus shigelloides, and the production process of liquid enzyme preparation adopts the steps of slant culture, seed liquid shaking, seed fermenter, fermenter, bacterial collection, mechanical cell crushing, clear liquid collection, (NH4)2SO4 fractional precipitation, HEPES buffer solution suspension, dialysis, and crude enzymes production. Enzyme dry powder preparation which is easy to be stored or transported can also be further refined, and processing steps added on the basis of the crude enzymes production are as follows: DEAE-Sephadex-A50 anion column chromatography, CM Sepharose Fast Flow cation column chromatography, dialysis, cryodesiccation and enzyme dry powder production. The direct application of the liquid crude enzymes preparation can ensure the organic phosphorus pesticide residue quantity in agricultural crops to be reduced by more than 90 percent, and the residual on the surface of garden spgarden stuff washed to be reduced by about 98 percent; after the enzyme dry powder preparation is diluted according to a specific proportion, the pesticide residue quantity can be reduced by more than 95 percent. The enzyme preparation product resolves the problem that the pesticide residue is over-standard in the agricultural production.

Description

technical field

[0001] The invention relates to a methyl parathion-degrading bacterium and a preparation method of an enzyme preparation thereof, which belongs to the field of biological high technology, uses microorganism technology to degrade chemical pesticides, and is suitable for the production and deep processing of green and pollution-free agricultural products in modern agricultural production. Household cleaning of pesticide residues in vegetables and fruits. Background technique

[0002] The use of chemical pesticides such as 666 and DDT has greatly promoted the development of modern agriculture, made an important contribution to the improvement of the output and quality of agricultural products, and greatly improved the labor efficiency and mechanization degree of agricultural production. However, these agrochemicals that are artificially synthesized and released into the environment have brought huge potential risks to human health and the ecological environment....

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