75 results about "Hepes buffer" patented technology

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention discloses a dual-emission molecular imprinting ratiometric fluorescence sensor, a preparation method thereof and application of the dual-emission molecular imprinting ratiometric fluorescence sensor in aureomycin detection, and belongs to the technical field of chemical sensors. The preparation method of the sensor comprises the following steps: preparing silicon dioxide-quantum dot composite nanoparticles, and preparing a molecularly imprinted polymer by taking salicylamide as a template molecule; and mixing the molecularly imprinted polymer and the silicon dioxide-quantum dot composite nanoparticles in an HEPES buffer solution by adopting a post-mixing strategy to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The double-emission molecular imprinting ratiometric fluorescence sensor has the beneficial effects that the double-emission molecular imprinting ratiometric fluorescence sensor provided by the invention is rich in fluorescence color change, rapid, high-sensitivity and visual detection of aureomycin can be realized, the whole detection process is simple, convenient and rapid, and the double-emission molecular imprinting ratiometric fluorescence sensor is suitable for being widely popularized in practical application.

The invention discloses a head and neck squamous cell carcinoma organ as well as a culture method, a culture medium and application thereof. The culture medium is prepared from the following components based on Advanced DMEM / F12: 1 percent of Antibiotic-Antibiotic, 1 percent of GlutaMAX, 1 percent of HEPES buffer solution, 50ng / ml of FGF-10, 50ng / ml of EGF, 1nM of bFGF, 500ng / ml of Wnt3a recombinant protein, 500ng / ml of Noggin recombinant protein, 0.1 mu g / ml of R-spondin-1 recombinant protein, 1mM of N-acetylcysteine, 10mM / ml of nicotinamide and 1x of B27 supplement. The culture medium and the culture method disclosed by the invention are particularly suitable for culturing head and neck squamous cell carcinoma organoid, and the obtained organoid has important significance for researching tumor generation, a unique invasive growth mechanism, prognosis, therapeutic targets, drug screening and the like, and has wide application prospects.

The invention discloses a tissue preservation solution in organoid construction as well as a preparation method and application thereof. The tissue preservation solution is prepared from the following components: an Advanced DMEM / F12 culture medium, a mycillin / amphotericin B three-antibody mother solution, Glutamax-1, an HEPES buffer solution and a Y-27632 mother solution. The preparation method of the tissue preserving fluid comprises the following steps: adding an Advanced DMEM / F12 culture medium into a centrifugal tube on a super clean bench; then sequentially adding a mycillin / amphotericin B three-antibody mother solution, Glutamax-1, an HEPES buffer solution and Y-27632, and uniformly mixing, so as to obtain the tissue preservation solution. After uniformly mixing, filtering and sterilizing by using a disposable needle type filter, and collecting the preserving fluid into a centrifugal tube; the invention also discloses an application of the tissue preserving fluid in organoid construction. According to the tissue preserving fluid obtained by the invention, in the transportation process, the cell viability in the tissue is improved, the cell damage is reduced, and the success rate of primary cell adherent culture and organoid construction is increased.

The invention relates to the technical field of plasmalogen, and discloses a preparation method and application of an amide plasmalogen compound, and the preparation method comprises the following steps: (1) reacting a compound 1 and a compound 2 in an HEPES buffer solution to obtain a compound 3; (2) reacting the compound 3 in an ethyl acetate phase and a water phase by using phospholipase D under the assistance of Ca < 2 + > ions to obtain a compound 4; (3) expression and purification of CarF protein from myxobacteria; and (4) reacting the compound 4 and CarF protein in a Tris-HCl buffer solution under the assistance of NADH (Nicotinamide Adenine Dinucleotide) and catalase to obtain a compound 5. According to the invention, the purified recombinant myxobacteria CarF protein and the chemically synthesized CarF protein reaction substrate are adopted to finally synthesize the amide plasmalogen, and the amide plasmalogen can show the effect similar to that of natural plasmalogen.

The embodiment of the present invention discloses an induction culture medium, method and application for improving the viability and factor secretion ability of hair follicle-derived mesenchymal stem cells in a hypoxic environment. The induction culture medium includes a basal culture medium and an induction drug, and the induction drug includes transforming growth factor β, hepatocyte growth factor, cyclic adenosine monophosphate, triptolide, sodium hydrosulfide, lipopolysaccharide, hyaluronic acid, dexamethasone, L-glutamine and HEPES buffer. The induction culture medium provided by the present invention can promote the metabolism and energy production of hair follicle-derived mesenchymal stem cells, enhance the antioxidant capacity of cells, improve the survival rate and factor secretion ability of cells in a hypoxic environment, and solve the problems of low cell viability, slow growth, low cytokine secretion, etc. after transplanting cells into damaged tissue areas in the prior art.

The invention provides a hemicyanin probe compound, and establishes a method for detecting sulfite ions and sulfur ions in a solution by probe molecules under ultraviolet visible and fluorescent conditions. Common anions are respectively added into a C2H5OH-Hepes buffer solution, only sulfite and sulfide ions are changed, the sulfite ions can fade the solution, the fluorescence intensity of the solution is weakened and the anti-interference performance is good, and the sulfide ions can deepen the color of the solution, enhance the fluorescence intensity of the solution and have good anti-interference performance. Meanwhile, the residual quantity of sulfur dioxide in the stoving traditional Chinese medicinal materials is taken as a detection target, the stoving traditional Chinese medicinal materials are treated under an alkaline condition, and ultra-sensitive and rapid detection of the stoving traditional Chinese medicinal materials is realized through a standard curve method. The semi-cyanin probe compound has the advantages of high analysis speed, stable fluorescence performance, real-time detection and the like when being used for determining the content of sulfur dioxide residues in traditional Chinese medicinal materials, and has popularization and application values in sulfur dioxide residue detection in the fields of traditional Chinese medicines, foods and the like.

This invention discloses a dual-emission molecularly imprinted ratiometric fluorescence sensor, its preparation method, and its application in the detection of chlortetracycline, belonging to the field of chemical sensor technology. The preparation method of this sensor includes the following steps: preparing silica-quantum dot composite nanoparticles; preparing a molecularly imprinted polymer using salicylamide as a template molecule; and using a post-mixing strategy to mix the molecularly imprinted polymer and silica-quantum dot composite nanoparticles in HEPES buffer to construct the dual-emission molecularly imprinted ratiometric fluorescence sensor. The advantages of this invention are: the dual-emission molecularly imprinted ratiometric fluorescence sensor provided by this invention exhibits rich fluorescence color changes, enabling rapid, highly sensitive, and visual detection of chlortetracycline; and the entire detection process is simple, convenient, and fast, making it suitable for widespread application in practical applications.

The present invention relates to the field of biotechnology, and in particular to a positive quality control product for detecting group B streptococci and a preparation method thereof. The quality control product comprises an inactivated GBS strain, an internal standard plasmid, and a diluent; the inactivated GBS strain comprises at least one of types Ia, Ib, III, and V; and the diluent is composed of P300, P950, silicone, EDTA·2Na, and HEPES buffer. Experimental results show that the quality control product provided by the present invention has good stability, can be stably stored for more than 14 days at 37°C for thermal acceleration, and can be stably stored for at least 18 months at 2-8°C, with significantly better stability than existing products. The quality control product is consistent with actual GBS clinical swab samples and can be detected by marketed manufacturers as a standard quality control product. It can realistically simulate the in vitro nucleic acid detection process of GBS and has strong versatility. The product has the advantages of low cost, high stability, and easy storage and transportation, making it convenient for large-scale industrial production and has important value and application prospects.

The invention discloses a creatine kinase isoenzyme detection reagent, and belongs to the technical field of biological detection. The creatine kinase isoenzyme detection reagent comprises a reagent A and a reagent B, the reagent A is prepared from 30 to 60mM of a Hepes buffer solution, 0.2 to 0.5 g / L of mouse IgG (Immunoglobulin G), 1 to 5g / L of TritonX-100, 0.4 to 0.8 g / L of a stabilizer and 1 to 2g / L of a template separating agent; the reagent B is prepared from 30 to 60 mM of Hepes buffer solution, 1 to 3 g / L of sealing agent, 1 to 5 g / L of stabilizing agent and 1 to 3 g / L of polystyrene latex microsphere coupled with antibody. The template separating agent is added into the reagent A, so that active sites of CK-BB or CK-MM can be effectively hindered, the influence on CK-MB is reduced by selecting the reagent, and combination of CK-BB or CK-MM and a template is facilitated; after the polystyrene latex microspheres added with the magnetic coupling antibody in the reagent B are combined with the CK-MM, the content of an object to be detected is determined according to the change of the absorbance value of a sample, and the creatine kinase isoenzyme detection reagent is high in accuracy.

The embodiment of the invention discloses a pharmaceutical composition for improving cell therapy regeneration and application thereof. The pharmaceutical composition comprises 5 to 15 ng / mL of a transforming growth factor beta, 0.02 to 0.06 [mu] M of triptolide, 8 to 12 ng / mL of a vascular endothelial growth factor, 0.1 to 1 [mu] g / mL of a grape seed extract, 0.8 to 1.6 mg / mL of a trace element solution, 0.5 to 2% of hyaluronic acid, 0.05 to 0.15 [mu] M of cyclosporin and 15 to 25 mM of an HEPES buffer solution. The pharmaceutical composition provided by the invention can effectively promote proliferation and differentiation of hair follicle-derived mesenchymal stem cells, plays a better role in treating alopecia, has the advantages of small side effect, high safety, lower cost and wide applicable crowd, and has better application prospects in clinic.

The invention relates to the technical field of biological agents, in particular to a Michael addition reaction mediated polysaccharide and probiotic surface covalent coupling single cell encapsulation method. The method comprises the following steps: adding polysaccharide into dimethyl sulfoxide for dissolving, adding triethylamine and methacrylic anhydride to obtain modified polysaccharide containing alpha, beta-unsaturated ester, adding activated probiotic suspension into an HEPES buffer solution, adding a modified polysaccharide solution containing alpha, beta-unsaturated ester into the vortex-dispersed probiotic suspension, oscillating and incubating to obtain the probiotic suspension containing alpha, beta-unsaturated ester. Preparing a probiotic suspension of the nano coating encapsulated by the polysaccharide; according to the method, a stable and firm protective layer is formed on the surface of the probiotics, the activity and functions of natural bacteria are kept, meanwhile, the conveying and colonization survival efficiency of the probiotics in the intestinal tract is effectively improved through a compact polysaccharide structure on the surface of the probiotics, and the commercial application value of the probiotics in the fields of food and medicine is improved.

The application belongs to the technical field of pharmaceutical preparations, and particularly relates to a liposome of irinotecan hydrochloride, a preparation process thereof and application. The preparation process comprises the following steps: (1) adjusting the concentration of sucrose octasulfate triethylamine and total lipids in an inner water phase, mixing the oil phase and the water phase according to certain flow rates and proportions through a microfluidic device to prepare blank liposomes; (2) using a HEPES buffer solution to remove free sucrose octasulfate triethylamine and organic solvents outside the blank liposomes, and concentrating; (3) using an active drug loading method to prepare drug-loaded liposomes. The scheme can greatly improve the encapsulation efficiency of the irinotecan hydrochloride liposome and accurately control the liposome particle size, and the finally prepared liposome particle size can be 90 nm-115 nm, the encapsulation efficiency reaches 95%, and the liposome has more excellent stability.

The embodiment of the invention belongs to the technical field of detection, and relates to a kit for detecting creatine kinase isoenzyme and application, the kit comprises a first detection reagent and a second detection reagent; the first detection reagent comprises a Hepes buffer solution, NaCl, PEG-6000 (Polyethylene Glycol), nonylphenol polyoxyethylene ether, BSA (Bovine Serum Albumin), NaN3, a chemical blocking agent and a biological blocking agent; the second detection reagent is prepared from CKMB sensitization latex particles, a Hepes buffer solution, a sealing agent, tween-20 and NaN3. According to the application, the detection sensitivity of the creatine kinase isoenzyme is effectively improved.

The application relates to the technical field of medical nanobiomaterials, and provides a preparation method of a GOX-CAT-HAase-Hb natural enzyme nanogel for pancreatic cancer starvation treatment, which solves the key problems of complex nanocarrier process, low enzyme encapsulation rate, insufficient drug loading, poor dispersibility, easy degradation and inactivation of free enzymes, low synergistic efficiency and poor biocompatibility in the pancreatic cancer starvation treatment; the method comprises the following steps: S1, polyallylamine is dissolved in a HEPES buffer solution, PEG2000-NHS is reacted at room temperature, glycidyl methacrylate is reacted at 60 DEG C, and a positive polymer solution is obtained through dialysis; S2, GOX, HAase, CAT and Hb are respectively modified by NAS acrylation, and are freeze-dried after dialysis and purification; S3, the four are mixed in a molar ratio of 1:1:1:1, positive polymer and acrylamide monomers are added, an initiator is added after nitrogen deoxygenation, polymerization is carried out, and a natural enzyme nanogel is obtained through dialysis and purification.

The invention discloses a preparation method and application of an aromatic dicarboxylic acid-terbium complex fluorescent sensor array. The fluorescent sensor array comprises three fluorescent probes, the terbium complexes are respectively a 5-methoxy isophthalic acid-terbium complex, a 5-hexadecyloxy isophthalic acid-terbium complex and a 5-(6 '-cholesterol) hexyloxy isophthalic acid-terbium complex, and the preparation method comprises the following steps: (1) dissolving an aromatic dicarboxylic acid ligand in DMSO (dimethylsulfoxide) to obtain an aromatic dicarboxylic acid ligand mother solution; dissolving a terbium source in distilled water to obtain terbium ion mother liquor; and (2) adding the terbium ion mother liquor into an HEPES buffer solution, adding an aromatic dicarboxylic acid ligand mother liquor into the HEPES buffer solution, and carrying out constant-temperature reaction at 30-40 DEG C for 40-95 minutes to obtain the aromatic dicarboxylic acid-terbium complex. The fluorescent sensor array can be used for detecting tetracycline, cefixime, chloramphenicol and ciprofloxacin, and is high in accuracy and sensitive.

The present invention discloses a zinc ion fluorescent probe based on 8-aminoquinoline, its preparation method and application. The zinc ion fluorescent probe based on 8-aminoquinoline has the following structural formula. The present invention uses 8-aminoquinoline as a matrix, introduces an acetylamino group at position 2 and reacts with salicylaldehyde at position 8 to design and synthesize a highly selective zinc ion fluorescent probe. 2+ The fluorescent probe APYQ is not interfered by other metal ions, and its synthesis steps are simple and the purification process is easy. The fluorescent probe of the present invention has excellent detection limit (1.87nM) and response time (5s). These two indicators are better than other Zn 2+ The fluorescent probe of the present invention can be used to detect Zn with naked eyes in ethanol / HEPES buffer solution and on test paper. 2+ , can also be used to detect Zn in Hela cells 2+ Fluorescence imaging has good application prospects.

The present invention discloses a fluorescent antibody sealing solution, a preparation method and uses thereof. The fluorescent antibody sealing solution is mainly composed of 5% - 20% trehalose, 0.1% - 1.0% BSA, 0.1% - 1.0% polyvinylpyrrolidone, 0.1% - 5.0% sorbitol, and the rest is HEPES buffer solution by mass percentage. This sealing solution is suitable for sealing fluorescent microsphere antibodies; when used for sealing fluorescent dye antibodies, it also includes 0.05% - 0.1% sodium hyaluronate. The preparation method includes first preparing 20 mmol / L HEPES buffer solution, adjusting the pH to 7.4 - 7.6 with 1 mol / L sodium hydroxide, and then successively adding the substances in the sealing solution formula and dissolving them fully. The beneficial effects of the present invention are that the sealing solution has a simple formula and is convenient to prepare. After the fluorescent antibody sealed by it undergoes a labeling reaction, it can break free from the bondage and flow with the sample, and can maintain the antibody activity for a long time.

The invention belongs to the technical field of karyotype analysis of chromosomes, and particularly relates to a cell pretreatment solution for karyotype analysis and preparation of chromosomes and application of the cell pretreatment solution. The cell pretreatment solution comprises the following components: 15 mmol / L Hepes buffer solution with the pH value of 7.2-7.6, 0.08-1.2 [mu] g / mL of vinblastine, 0.1-0.5 v / v% of Triton X-100 and 1-2 mmol / L of EDTA (Ethylene Diamine Tetraacetic Acid), and is a cell pretreatment solution with low toxicity, better dyeing effect and good cell type universality. When the cell pretreatment liquid is used for preparing a chromosome specimen, the preparation time is remarkably shortened, not only can more metaphase cell division phases be obtained, but also the proportion of the division phases capable of being used for chromosome analysis is greater than 83%, and the efficiency of karyotype analysis is remarkably improved. Meanwhile, the prepared chromosome specimen is clear in structure number, good in form, high in dispersity and few in background impurities, and the accuracy of chromosome karyotype analysis is remarkably improved.

A method for preparing a fluorescent probe for serial detection of copper ions and cysteine ​​and its application relate to the field of analytical detection of copper ions and cysteine, and in particular to a method for preparing a 1,8-naphthalimide fluorescent probe L and serial detection of copper ions and cysteine. The method can solve the shortcomings of low sensitivity and susceptibility to glutathione in existing cysteine ​​detection methods. The preparation method of probe L in the present invention is: 4-hydrazino-N-(2-hydroxyethyl)ethoxy-1,8-naphthalimide and quinoline-2-formaldehyde are refluxed in anhydrous ethanol to prepare fluorescent probe L. The fluorescent probe L prepared by the present invention can continuously realize specific fluorescence "on-off-on" detection of copper ions and cysteine ​​in a CH3CN / HEPES buffer solution system with a volume ratio of 3:7, and has good selectivity during the detection process and can resist interference from various factors. The present invention can be used for qualitative and quantitative detection of cysteine ​​in milk.

This invention belongs to the field of exosome preservation technology and discloses a stabilizer composition for exosome cryopreservation and its application. The stabilizer composition for exosome cryopreservation provided by this invention consists of the following components and their amounts: phytosphingosine: 0.1%-0.2%; cerebroside sulfate: 0.05%-0.1%; trehalose: 2%-5%; lipid-soluble antioxidant: 0.01%-0.05%; HEPES buffer: 10-20 mM; metal ion chelating agent: 0.01%-0.05%. The composition of this invention forms a "gradient protection" through the membrane insertion of phytosphingosine and the shallow negative charge shielding of cerebroside sulfate, enabling stable cryopreservation of exosomes for more than one week with a bioactivity retention of >85%. This preservative is animal-free, easy to use, and effective, making it suitable for the clinical translation and practical application of exosomes.

The invention provides application of a cross-linking reagent in capturing nucleic acid of a biological sample fixed by an aldehyde reagent. The decrosslinking reagent comprises a denaturing agent and a buffer solution; wherein the denaturant is selected from at least one of a compound shown as a formula (I) and salt substances thereof; the buffer solution is selected from at least one of a sodium citrate-sodium chloride buffer solution, a phosphate buffer solution, a Tris-HCl buffer solution and an HEPES buffer solution. The decrosslinking reagent can eliminate connection between protein and nucleic acid in a biological sample fixed by an aldehyde reagent, unfold a nucleic acid chain and reduce the melting temperature, so that the capture efficiency of cells and the capture number of genes are improved.

The invention discloses a reconstitution fluid of a fluorescent microsphere labeled antibody and application of the reconstitution fluid in preparation of a lipid arabinomannan fluorescent microsphere immunochromatography test strip. The reconstitution fluid of the fluorescent microsphere labeled antibody comprises an HEPES buffer solution, bovine serum albumin, trehalose, sucrose and Pluronic L64. The reconstitution fluid of the fluorescent microsphere labeled antibody can obviously enhance the stability of the fluorescent microsphere labeled antibody, effectively avoids the reduction of the activity and the detection sensitivity of the fluorescent microsphere labeled antibody in a short-term storage process, reduces the batch-to-batch difference, and has a wide application prospect in large-scale production of fluorescent microsphere immunochromatography test strips.

The invention belongs to the technical field of biological detection, and particularly relates to a heparin binding protein detection kit as well as a preparation method and application thereof. The kit comprises a sample treating agent, a reagent R1, a reagent R2, a calibration product, a quality control product, matched excitation liquid, cleaning liquid and the like. The sample treatment agent contains a multi-component synergistic system including an HEPES buffer solution, carrageenan, polyoxyethylene octyl phenyl ether and the like, the reagent R1 is streptavidin modified magnetic nanoparticles, and the reagent R2 is an NSP-DMAE-NHS labeled HBP antibody. The kit is based on a magnetic particle chemiluminescence method, supports direct detection of plasma and whole blood, and does not need complex pretreatment. The kit is high in detection sensitivity, strong in anti-interference capability and excellent in intra-batch and inter-batch precision, can rapidly and accurately quantify the HBP concentration, can be used for early diagnosis of infectious diseases, illness state monitoring and treatment guidance, is adaptive to emergency treatment, bedside detection and other scenes, is controllable in cost and convenient to operate, and has important clinical application value.

The invention belongs to the field of chemical detection, and discloses a fluorescent probe P1, a preparation method thereof and application of the fluorescent probe P1 in spermidine and viscosity dual-function detection. The structural formula of the fluorescent probe P1 is shown in the specification. The recognition performance of the probe P1 in various common analytes in a DMF-HEPES buffer system is researched through a fluorescence spectrophotometer. Results show that the probe P1 has specific fluorescence recognition performance on spermidine, can overcome interference of other common analytes, and has a wide pH value application range. The probe has relatively good sensitivity on the recognition of spermidine, and the lowest detection limit is 0.213 mu M. In addition, the probe P1 has a good response effect on the viscosity of the solution, is not interfered by the polarity of the solution, and can realize quantitative detection of the viscosity. The results show that the probe P1 has wide application prospects in the fields of food safety, environmental detection and biology.

The invention provides a diluent for detecting a cardio-pulmonary function target substance in a fingertip blood sampling mode as well as a preparation method and application of the diluent, and belongs to the technical field of medical in-vitro diagnosis. According to the formula of the diluent, an HEPES buffer solution is taken as a basic solvent, and the diluent comprises the following components in percentage by mass: 1 to 4 percent of cane sugar, 1 to 3 percent of glucose, 2 to 4 percent of trehalose, 1 to 3 percent of BSA (Bovine Serum Albumin), 0.9 to 1.5 percent of NaCl, 0.2 to 0.4 percent of HIER-R-012, 1 to 5 percent of 2-methacryloyloxyethyl phosphorylcholine, 0.1 to 0.5 percent of PEG6000, 6 to 10 percent of hydroxypropyl-beta-cyclodextrin, 0.05 to 0.1 percent of preservative, 0.1 to 0.5 percent of tween-20 and 0.1 to 0.5 percent of S11. The required blood sampling amount is reduced to 50 microliters by accurately regulating and controlling the dilution ratio and proportion, and the method has the characteristics of reduction of non-specific binding, high sensitivity and strong stability.

The application relates to the technical field of fluorescent probe, in particular to a fluorescent probe compound for detecting Cu 2+ and a preparation method and application method thereof. The application provides a fluorescent probe compound DM-2 for detecting Cu 2+ and a preparation method and application method thereof; the probe DM-2 emits weak green fluorescence in a weak alkaline HEPES buffer solution; after Cu 2+ is added, the fluorescence intensity at 600 nm is rapidly increased, and reaches stability in 10 min; the main advantages of the probe are as follows: first, the probe DM-2 has the properties of a ratio type probe and has a large stokes shift; second, after the probe reacts with Cu 2+ , the solution color changes from colorless to yellow, and the fluorescence color changes from green to orange, so that visual detection and analysis of Cu 2+ can be realized; and third, the fluorescent probe has simple synthesis steps, high yield and good stability.

The invention provides a preparation process of a Hep-2 cell extract suitable for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies and a preparation method of the test strip. The method comprises the following steps: transferring cultured Hep-2 cells into a PBS (Phosphate Buffer Solution), carrying out centrifugal separation on the obtained Hep-2 cell suspension to obtain Hep-2 cell agglomerates, carrying out ultrasonic treatment on a mixture of the Hep-2 cell agglomerates and the extraction buffer solution in an ice bath, centrifuging the mixture subjected to ultrasonic treatment, collecting supernatant, and passing the supernatant through a micro-filtration membrane to obtain filtrate, namely the Hep-2 cell extract. The extraction buffer solution is an HEPES buffer solution containing isopropanol, trehalose, bovine serum albumin and phenylmethylsulfonyl fluoride. The prepared HEP-2 cell extract is used for preparing a fluorescence immunochromatography test strip for detecting antinuclear antibodies, and the detection sensitivity, the specificity and the reliability of a detection result can be considered at the same time.

The invention provides a homocysteine detection kit and a preparation method thereof, and belongs to the field of in-vitro diagnostic reagents, the homocysteine detection kit provided by the invention comprises a first reagent, a second reagent, a third reagent and a calibrator, the first reagent is an AMPSO buffer solution containing TCEP, adenosine, an antioxidant, a macromolecular coagulant and a preservative; the second reagent is an HEPES buffer solution containing a recombinant S-adenosine-L-homocysteine hydrolase, an S-adenosine-L-homocysteine coupled bovine serum albumin compound, a protein substance, a carbohydrate substance, glycerol, a surfactant and a preservative; the third reagent is a latex particle preservation solution marked with a mouse anti-human SAH antibody; the calibrator is a plurality of homocysteine solutions or homocysteine dry powder with different concentration levels and the concentration of 0-100 [mu] mol / L. The kit provided by the invention has the advantages of convenience and simplicity in use and high stability.

The present invention relates to the field of drug delivery technology, and in particular to a lipid nanoparticle lyophilized preparation and its preparation method and application. The present invention screens a suitable lyophilized preparation prescription, including the type and concentration of a lyoprotectant, the type and concentration of a buffer, and a lipid nanoparticle prescription, to obtain a lipid nanoparticle lyophilized preparation having a concentration of 10%-20% w / v sucrose as a single-component lyoprotectant, a concentration of 1-20mM Tris buffer or HEPES buffer as a buffer system, and a specific ionizable lipid. The lyophilized preparation is simple to prepare, maintains excellent physicochemical properties and in vivo transfection efficiency after reconstitution, and has good prospects in the development and application of nucleic acid drugs.