145 results about "Hepes buffer" patented technology

The invention relates to a synthesis method and application method of a benzothiazole di-Schiff base fluorescent molecular probe for detection of iron ions. The fluorescent molecular probe is obtainedthrough condensation of benzothiazole-2-formaldehyde and 2-hydroxy-1-naphthylhydrazine, and the fluorescent molecular probe is dissolved in a mixed liquid of dimethyl sulfoxide and an HEPES buffer solution so as to obtain a fluorescent molecular probe solution, wherein the pH value of the HEPES buffer solution is 6-8, and the volume ratio of methyl sulfoxide to the HEPES buffer solution in the mixed liquid is 3:7, and the concentration of the fluorescent molecular probe in the mixed liquid of 3:7 is 7-10 [mu]mol/L; a to-be-detected sample of 20 [mu]mol/L is added to the taken fluorescent molecular probe solution, and even mixing is performed so as to obtain a sample solution. The synthesis method and application method have the advantages that high-selectivity identification of Fe<3+> inthe water environment system can be realized, no interference is caused by other metal ions in the water solution, and a high anti-interference ability is achieved.

The present invention provides a device and a process for detecting an analyte in a biological fluid. The device comprises a separating matrix for separating analyte from the fluid and means for detecting the analyte, where the separating matrix contains HEPES buffer, preferably in an amount between 70 and 150 millimolar. The process comprises applying the sample to the device having a separating matrix and then detecting analyte in the sample using the detection means. The presence of HEPES in the separation layer shortened the detection time.

The invention relates to a testing reagent for activated partial thromboplastin time. The testing reagent is prepared from phospholipids, activator and stabilizer. The stabilizer is prepared from, by mass, 1%-4% of alanine and 0.5-5 mM of catechin. A preparation method of the testing reagent includes the steps that an HEPES buffer solution is prepared, the pH value of the buffer solution is adjusted to be neutral, and sodium chloride is added; the activator is added to the buffer solution, phospholipids and other components are added after stirring and even mixing are conducted, and then the testing reagent for APTT is obtained after even mixing, stirring and filtering are conducted. According to the testing reagent for activated partial thromboplastin time, stabilizer components with high toxicity are removed, so that production and use processes are safer; meanwhile, the stability and detection accuracy of the testing reagent for APTT are greatly improved.

The invention relates to method and agent box for detecting mammary gland globulin in the field of biology chemistry. The latex fixation leptospiral method comprises the steps of: a) mixing the mammary gland globulin antibody marked emulsion particle with the tested sample, b) quoting the test result by weather it has agglutination reaction, wherein the mammary gland globulin antibody marked emulsion particle is the emulsion particle which uses mammary gland globulin antibody R028 or R048 marked emulsion particle with the dilemma 2-10um; preserving the marked emulsion particle inside the HEPES buffer solution with pH 8.2. It also discloses a method for using the anti-mammary gland globulin monoclonal antibody and skin factor mark anti-mammary gland globulin monoclonal antibody to quantity test the mammary gland globulin by double clamp heart enzyme immune adsorption method. It also discloses a RT-PCR test mammary gland globulin expressing method and provides the agent box used to test the mammary gland globulin.

The invention provides a method for detecting hypochlorite. The method can quantitatively detect hypochlorite based on 2-pyridylaldehyde fluorescein hydrazone (FHP) and Cu<+>. The method is concretely characterized in that the Cu<+> is oxidized into Cu<2+> by the hypochlorite in an HEPES buffer solution with the pH of 7, and a complex is prepared from the Cu<2+> and the FHP, so a change in an ultraviolet-visible spectrum appears, thereby the detection of the hypochlorite is realized. The detection method has the advantages of high sensitivity and selectivity to the hypochlorite, simple, sensitive and rapid detection process, and accurate detection result.

The invention relates to the fields of cytobiology and cell culture, and particularly relates to a breast cancer organoid culture kit. A method for realizing the kit comprises the following steps: (1)preparing, sub-packaging and using a breast cancer organoid culture medium A; (2) preparing, sub-packaging and using a breast cancer organoid culture medium B; and (3) preparing, sub-packaging and using a sample preserving fluid. The breast cancer organoid culture medium A is a DMEM/F12 culture medium containing 100 U/ml of penicillin, 0.1 mg/ml of streptomycin, 1% of HEPES and 1% of Gluta Max 100 X; the organoid culture medium B is a DMEM/F12 culture medium containing EGF, an FGF growth factor, an ALK inhibitor A83-01, Noggin, an HEPES buffer solution, Y27632 and one or more of a Wnt signalpath related secretory protein family R-Spondin 1-4; and the sample preserving fluid is a DMEM/F12 culture medium containing 100 U/ml of penicillin, 0.1 mg/ml of streptomycin and 1% of L-glutamine. The three components form the breast cancer organoid culture kit.

The invention discloses a production method of quality control products of a blood gas analyzer, which comprises the following steps of: preparing a quality control liquid which includes an HEPES buffer solution with concentration of 30 to 50mmol/L; preparing an equilibrium gas which is a mixed gas of CO2, O2 and nitrogen gas; connecting the prepared quality control liquid and equilibrium gas into the corresponding pipelines respectively, meanwhile filling the liquid and gas into an ampoule bottle; and drawing out the pipelines from the ampoule after the volume of quality control liquid filled into the ampoule bottle reach the required level, meanwhile using high temperature flames to seal the bottle. The method is to fill the mixed gas of O2, CO2 and N2 by a predetermined ratio to the ampoule bottle, add the prepared quality control liquid while filling gas, and seal the bottle in the meanwhile. Therefore, the produced quality control substances will not change the predicted gas concentration level due to bottle sealing to a great extent, so the quality of the produced quality control products is reliable.

The invention discloses a method for detecting the total heavy metal content in medicinal materials. The method comprises the steps of firstly, building a mold for detecting the total heavy metal content in medicinal materials, and obtaining an equation of a linear standard curve I and an equation of a linear standard curve II; then, determining a fluorescence intensity value of a reaction system after medicinal materials to be detected are mixed with an HEPES buffer solution and a fluorescence probe solution to have a reaction; substituting the fluorescence intensity ratio I450/I380 into the equation of the linear standard curve to obtain a metal ion concentration C value in the medicinal material to be detected; substituting the metal ion concentration C value in the medicinal material to be detected into the equation of the linear standard curve II, and obtaining the total metal content in the medicinal material. According to the method, the heavy metal ions in the medicinal material are combined with a fluorescence probe, and the medicinal materials are calibrated through fluorescence; by means of the advantage that the fluorescence probe is flexible and rapid in reaction, the total heavy metal content in the medicinal materials to is accurately detected, operation is easy and convenient and consumed time is short.

The invention relates to a cornea mid-term preservation liquid. 1000ml of preservation liquid contains 0.5-1.5g of DMEM culture medium, 1-10g of hydroxypropyl methylcellulose, 0.1-10ml of 2-mercaptoethanol, 10-20g of dextran (with the molecular weight of 40000), 1-3ml of nonessential amino acid, 10-32mmol of Hepes buffer solution and 0.01-0.05mmol of dexamethasone, 50-70mg of gentamicin. The pH value of the preservation liquid is 7.2-8.0, and the permeation pressure is 350-400mmol/LH2O. The cornea mid-term preservation liquid not only has good cornea storage quality, but also has the advantage of good cost performance and is the cornea mid-term preservation liquid which is widely applicable to the eye bank at home and abroad.

The invention discloses a non-viral gene carrier of autofluorescence degradable poly-citrate and a preparation method thereof. Citric acid, 1,8-octylene glycol and polyethylene glycol are subjected to thermal polymerization to obtain a POCG prepolymer; then, the POCG and PEI (polyethyleneimine) are subjected to catalytic polymerization to obtain a POCG-PEI polymer; the POCG-PEI polymer and various genes (DNA/siRNA/miRNA) can form a stable nanocomposite in HEPES buffer liquid. The used thermal polymerization method has the advantages that the environment is protected; the operation is convenient; the raw material cost is low. The prepared POCG-PEI has good biocompatibility and high gene transfection efficiency; meanwhile, the POCG-PEI also shows certain fluorescence characteristics and optical stability, so that the polymer has good application prospects in the gene treatment process.

The invention discloses fluorescent recognition copper ion (CU2+) sensor molecules and belongs to the field of chemical synthesis and cation detection. DMF as solvent, glacial acetic acid as a catalyst and N,N-diethyl salicylic aldehyde and naphthalene diamine as a substrate are subjected to a reflux reaction and then are subjected to cooling, suction filtration, washing and recrystallization, and then brown yellow crystals are obtained. Experiments show that in a DMSO/H2O HEPES buffer system for the sensor molecules, only CU2+ can be added to quench green fluorescence of the system under an ultraviolet lamp, but other cations cannot quench fluorescence of the system when added; only CU2+ can enable the color of the system to change from yellow to colorless, but other cations cannot enable the color of the system to change when added. Thus, the sensor can be used for quick naked-eye detection of CU2+, the lowest fluorescence detection limit of the sensor can reach 3.37*10-8M, and the other cations have no obvious interference on recognition of CU2+.

The invention relates to the technical field of serum phospholipid detection and particularly relates to a serum phospholipid detecting reagent. A reagent R1 comprises a buffer solution, phospholipase D, DAOS, ascorbic acid oxidase, bilirubin oxidase, polyethylene glycol 6000, cane sugar, xanthan gum, mannitol, trehalose, BSA, glycerol propoxylate-block-ethoxylate (Pluranic L64) and an aseptic. A reagent R2 comprises the buffer solution, 4-aminoantipyrine, peroxidase, choline oxidase, the polyethylene glycol 6000, the cane sugar, the xanthan gum, the mannitol, the trehalose, the BSA, the glycerol propoxylate-block-ethoxylate (Pluranic L64) and the aseptic. The HEPES buffer solutions and a novel Trinder reaction chromogen DAOS are adopted. A plurality of stabilizers are added to obviously improve stability of the detecting reagent. The bilirubin oxidase and the ascorbic acid oxidase are added, thus effectively avoiding interference caused by bilirubin and ascorbic acid and greatly improving interference-resisting capability of the detecting reagent. In addition, addition of the glycerol propoxylate-block-ethoxylate (Pluranic L64) which is an optional nonionic surfactant prevents reaction system turbidity, enhances substrate stability and improves the interference-resisting capability of the detecting reagent.

The invention discloses a fluorescent aptamer probe based on Prussian blue nanoparticles as well as a preparation method and application of the fluorescent aptamer probe. The fluorescent aptamer probeis composed of Prussian blue nanoparticle complex modified FAM fluorophore aptamers. The preparation method comprises the following steps: incubating the Prussian blue nanoparticles and the aptamersmodified with the FAM fluorophore in an HEPEs buffer solution system in a dark place, and closing by adopting BSA, thereby obtaining the fluorescent aptamer probe. The fluorescent aptamer probe can beused for detecting various markers such as markers of tumor, breast cancer, blood glucose and Alzheimer's disease, has the advantages of strong signal, high specificity, high sensitivity, wide detection concentration range, good biological safety and the like, and is beneficial to popularization and application.

The invention discloses a preparation method of a zearalenone ratio fluorescence probe. The method comprises the following steps: (1) preparation of a silicon quantum dot: APTES((3-aminopropyl)-triethoxysilane) and sodium citrate are subjected to a reaction in glycerol, and silicon quantum dots are obtained; (2) preparation of CdTe (cadmium telluride) quantum dots: tellurium powder, sodium borohydride and the like are subjected to a reaction, a NaHTe (sodium hydrogen telluride) precursor is obtained and added to the reaction solution, and red CdTe quantum dots are obtained; (3) preparation ofaptamer grafted silicon quantum dots: the prepared silicon quantum dots, N-(3-dimethylaminopropyl)-N-carbodiimide hydrochloride, N-hydroxysuccinimide, and a zearalenone aptamer are subjected to a reaction in an HEPES buffer solution, and silicon fluorescence quantum dots with surface grafted with the zearalenone aptamer are obtained; (4) synthesis of the ratio fluorescence probe: the red CdTe quantum dots are mixed with the silicon fluorescence quantum dots with surface grafted with the zearalenone aptamer, and the fluorescence probe is obtained. The fluorescence probe is high in selectivity and sensitivity, simple to prepare and low in cost.

The invention relates to a method for using ytterbium ion and catechol violet as developer to detect the content of phosphate radical in urine without being diluted in the HEPES buffer sotution with pH value 7.0 directly, qualitatively and quantitatively. The process is simple, convenient, and the result is accurate, the method has a high sensitivity, and it is not disturbed by the other elements in urine, and it also can be applied to clinic.

The invention discloses a preparation method of gold nanoparticles. The preparation method comprises the following steps that a chloroauric acid solution with the concentration of 0.05-10 mmol/L and a HEPES buffer solution with the concentration of 5-50 mmol/L are prepared respectively, and the PH value of the HEPES buffer solution is adjusted to be 7.0-8.0 through sodium hydroxide; surfactants are added into the HEPES buffer solution, and a surfactant solution with the concentration of 1-2 mmol/L is prepared; the prepared chloroauric acid solution is slowly added into a reaction tank according to the mole ratio of 1:1-1: 10 of the chloroauric acid solution and the surfactant solution, and is stirred for 5-30 min at a constant speed of 200-300 r/min, and a mixed solution containing nano gold colloid is obtained; dry purification of the nano gold colloid is carried out on the mixed solution obtained in the step S400, and the gold nanoparticles are obtained. The gold nanoparticles can be prepared at normal temperature, and are good in dispersity and homogeneous degree.

The invention provides a method for detecting oxalate in water solution. The method uses pyrocatechol violet as color developing reagent based on Cu<2+> ions and directly and quantificationally detects the oxalate in the water solution in HEPES buffer solution with a pH of 6.0, thereby the determining process is simple and convenient and the measuring result is accurate. The method has high sensibility, can not be interfered by other anions in the water solution, and can be applied to detection of the oxalate in clinic, foodstuff and environment.

The invention relates to acipenser baerii ovum short-term preserving fluid. One liter of the fluid comprises the following components: 120 mmol of sodium chloride, 5.67 mmol of potassium chloride, 0.78 mmol of magnesium sulfate, 0.65 mmol of calcium chloride, 4.24 mmol of glucose, 20 mmol of sodium bicarbonate, 1 gram of bovine serum albumin, 20 mmol of HEPES buffer salt, 200,000 IU of penicillin and 0.2 gram of streptomycin; and the pH value of the fluid is 8.0. The preparation method comprises: at normal temperature, preparing the preserving fluid by using the components; and adjusting the pH value of the fluid to 8.0 with 1mmol/L solution of NaOH. The acipenser baerii ovum short-term preserving fluid can realize the short-term preservation of acipenser baerii ova, namely preserving the ova at 16 DEG C for 4 hours for achieving a fertility rate of 86.36 percent, a hatching rate of 94.74 percent and an aberration rate of 0, and solves the technical problem caused by the asynchronous spawning inducing effects of female and male acipenser baerii.

The invention discloses a preserving fluid suitable for preserving virus samples at normal temperature and a preparation method thereof. The preserving fluid comprises 150-250 g of sodium chloride, 8-12 g of potassium chloride, 20-30 g of D-glucose, 3-5 g of sodium hydroxide, 0.4-0.6 g of phenol red, 2-4 g of dipotassium hydrogen phosphate, 1-1.5 g of disodium hydrogen phosphate, 8-9 g of sodium bicarbonate, 4-5 g of bovine serum albumin, 0.5-0.9 g of 2-methyl-4-isothiazoline-3-ketone hydrochloride and 0.1-0.5 g of polymyxin B sulfate. The invention relates to the technical field of clinical examination. According to the preserving fluid suitable for preserving virus samples at normal temperature and the preparation method thereof, the prepared preserving fluid has good preserving capability, can ensure that a sample virus still has activity after preservation for more than one week at normal temperature and can be preserved at 2-8 DEG C for a longer time, does not interfere a PCR detection result, has good antibacterial capability, has low requirements on production and use environments, does not need irradiation sterilization, is low in cost and easy to mass-produce, guarantees no decomposition of nutrients, and guarantees the stability of the pH value in cooperation with an HEPES buffer solution or a phosphate buffer solution.

The invention relates to cornea metaphase preserving fluid without a serum component. The cornea metaphase preserving fluid is prepared from an compound electrocular irrigating solution, sodium chloride, magnesium sulfate, monopotassium phosphate, chondroitin sulfate, sodium hyaluronate, a HEPES buffer solution, hydrochloric acid dexamethasone, reduced glutathione and levofloxacin. The cornea metaphase preserving fluid is reddish and transparent liquid with a specific electrolyte ionic concentration, a pH value, crystalloid osmotic pressure and colloid osmotic pressure. Needed raw materials are easy to get, the cornea metaphase preserving fluid does not contain the serum component, the risks of infecting special viruses is avoided, osmotic pressure of intracellular fluid and osmotic pressure of extracellular fluid are balanced through various ions, and the preservation effect is remarkable.

The invention discloses a fluorescein-rhodamine B copper ion ultraviolet molecular probe applied to naked eye detection and synthesizing and using methods thereof, relates to a Cu<2+> probe and synthesis and application thereof and aims at solving the technical problems that an existing Cu<2+> molecular probe needs to use an expensive-price high-precision fluorescence spectrophotometer for detection and is prone to being interfered by external environment. A structural formula of the molecular probe disclosed by the invention is shown in a following description. According to a preparation method of the molecular probe, the molecular probe is prepared by the steps of utilizing fluorescein mono-aldehyde and rhodamine B hydrazine to react and utilizing a mixed solvent of methylene dichlorideand methyl alcohol to separate through a column chromatography. A use method of the molecular probe comprises the steps of dissolving the probe in a mixed solution of MeOH and HEPES buffer solution, detecting an added sample to be detected and judging that Cu<2+> is contained if color of the solution is changed into deep pink from light pink; or detecting ultraviolet spectrum absorbance values before and after the sample is added and judging that the sample solution contains the Cu<2+> if the absorbance value is improved when the wavelength is 502 and 553nm. A detection method is simple, quickand visual.

The invention discloses a nano gene carrier for in-vivo targeting tumor imaging and treatment and a preparation method and application thereof. The method comprises the steps that 1, a PPF polymer isprepared; 2, a PPR polymer is prepared; 3, a nano gene carrier is prepared; 4, a PPFR polymer and a gene are added in an HEPES buffer solution, and then incubating in conducted in a water bath to formstable nanocomposites. by making certain tumor active targeting acceptor molecules or cell fluorescent molecule grafted to the PP polymer, and polymers are obtained. The obtained polymers have very good biocompatibility and good hydrophilicity, and a synthesis method of a thermal polymerization reaction and a catalytic reaction is environmentally friendly, easy to operate and low in cost of raw materials. Due to introduction of the certain tumor active targeting receptor molecules and cell fluorescent molecules, the targeting ability and biological imaging ability of the polymers for tumors are improved, and application of the polymers in biological imaging and the tumors is greatly improved.

The detection method for inorganic P content in milk by UV visible spectrophotometer in HEPES buffer with 6.5-7.5 pH value and Yb ion and pyrocatechol violet as developer is benefit to learn supply condition and improve production quality. This invention is simple and fit to clinic an industry.