17results about How to "Normal growth" patented technology

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

This invention relates to a halophilic Bacillus ( Halobacillus sp HMF09, with accession number CGMCC No. 30475, is an antagonistic strain against *Fusarium oxysporum* tomato-specific strain. It exhibits high salt and alkali tolerance and efficient cadmium ion fixation. Application of this strain can reduce the ionic activity of cadmium in soil, alleviate salt and alkali stress on crops, and promote crop growth.

This utility model belongs to the field of land ecological environment restoration technology, specifically disclosing a land ecological environment restoration device, including a U-shaped plate. A fixing lug is fixedly connected to the outer wall of the U-shaped plate, and a fixing pin is inserted into the inside of the fixing lug. Arc-shaped surfaces are formed at both ends of the U-shaped plate, and diversion holes are formed inside the U-shaped plate. A water storage component is connected to the U-shaped plate to collect rainwater for subsequent irrigation of vegetation. By setting up the water storage component, the water storage box can store water through the diversion channel and water inlet during rain. After a certain period of time after the rain stops, the seal on the drip hole is opened, allowing the water inside the water storage box to be discharged and used to irrigate the vegetation. This achieves the effect of storing water during rain and subsequently irrigating the vegetation, preventing vegetation from drying out and dying, allowing the vegetation to grow normally, stabilizing the soil on slopes, and restoring the ecological environment of slopes.

The utility model discloses a prefabricated ecological crown beam which comprises a front vertical plate, a rear vertical plate is arranged on the rear side of the front vertical plate, and the front vertical plate and the rear vertical plate are arranged in a front-back mirror image mode. A cross beam is arranged between the front vertical plate and the rear vertical plate, and the front vertical plate, the rear vertical plate and the cross beam are connected through connecting bolts; the integral structure of the crown beam is split into single components of the plate and the beam, so that a mold structure is very simple during production, and the manufacturing cost of the mold is very low; the crown beam can be split into single components, so that the crown beam can be split in the production process, and after the crown beam is split into the single components for production, the operation process becomes simpler, and the production efficiency becomes higher; in addition, the storage space of the single assemblies is reduced, the storage capacity of the same area is increased, and the site occupation cost is reduced.

The application discloses a deletion mutant of a rice LHCB5 gene and application thereof, and belongs to the field of gene editing. Through gene editing system, the 3'UTR region of the rice LHCB5 gene is precisely edited, and a 40bp homozygous deletion mutant of +16 to +55bp downstream of the termination codon TAA is obtained. The mutant can be stably inherited, and the growth phenotype is not significantly different from that of the wild type. Meanwhile, the resistance to rice blast and bacterial leaf blight is significantly improved, and the length of the disease spot is significantly reduced. The application first discloses the disease resistance regulation function of the 3'UTR of the LHCB5 gene, realizes the improvement of the disease resistance through the editing of the non-coding region, avoids the safety and agronomic trait risks brought by the transgene and the coding region editing, and the obtained mutant can be directly used for the breeding of the rice broad-spectrum disease resistance, and has important application value.

The application discloses Pseudomonas songnenensis, a microbial inoculum and application thereof, and relates to the technical field of environmental microorganisms and solid waste resource technology. The Pseudomonas songnenensis strain JP01 is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 37102. The strain has salt and alkali tolerance characteristics, and can degrade coal gangue and promote the release of effective phosphorus, available potassium and effective silicon nutrients. After inoculation for 4 days, under normal conditions, the effective phosphorus content of the strain JP01 treatment group is 758.65% higher than that of the CK group; under salt and alkali conditions, the effective phosphorus content of the strain JP01 treatment group is 4.70 times that of the bacillus megaterium treatment group. The strain has good long-term colonization ability in a coal gangue-salt and alkali soil mixed substrate, and the viable cell count can still be maintained at a high level of 2.72x10 7 CFU / g after 90 days of culture. The application is suitable for coal gangue resource utilization and salt and alkali soil improvement, and has good phosphorus element activation ability and functional stability in a salt and alkali environment.

The utility model relates to the technical field of breeding incubator, specifically to a kind of small goose breeding incubator, it includes: incubator and flat plate, and two three blocks are slidably connected in flat plate inner side, and the inner wall of both sides of incubator is uniformly provided with the clamping groove compatible with three blocks, three blocks outside is movably inserted in the inner side of clamping groove, and four blocks are fixedly connected in three blocks side, and four blocks side is rotatably connected with connecting rod, and the other end of two connecting rods is rotatably connected with five blocks, and five blocks inner side is screw-threaded with screw rod, and screw rod outside is rotatably connected in flat plate inner side, and two external threads of opposite rotation are set in screw rod outside, and multiple feeding bowls and water bowls are provided on flat plate top. The utility model, by the cooperation between block, three blocks and connecting rod, so that the user can conveniently fix flat plate with device, so that the user can quickly disassemble it, facilitate user to clean it, further improve the working efficiency of user.

The application discloses a kind of dynamic metabolic regulation escherichia coli fermentation preparation alpha-ketoisovaleric acid method, belong to the field of bioengineering.The recombinant escherichia coli constructed contains the dynamic regulation cell growth metabolic pathway and balance coenzyme circulation system established based on CRISPRi technology and degradation label;It can be with cheap glucose as carbon source, with inorganic salt as nitrogen source, and ferment to generate high value-added alpha-ketoisovaleric acid, under the fermentation strategy of two-stage fermentation, the yield of alpha-ketoisovaleric acid can reach 35.2g / L for 60h, with the prospect of industrial application.

The application discloses a slow-flowing river ecological restoration method, and relates to the technical field of river restoration.The method comprises the following steps: 1) preparing an ecological restoration carrier; 2) determining the ecological suitability of river bottom sludge; 3) dividing the river into different regions; and 4) restoring the ecology of the river.The present application divides the slow-flowing river with mild pollution into different regions according to the survival rates of aquatic plants, and uses different ecological management schemes accordingly.The survival rate of the seedlings of submerged plants in the slow-flowing river section after management is significantly improved, and about 60% of the riverbed is covered by the submerged plants.

This invention discloses an intelligent management method and system for agricultural greenhouses based on artificial intelligence, relating to the field of electrical data analysis technology. It includes coordinate planning processing of the locations of all monitoring devices inside the agricultural greenhouse. This invention calculates all monitoring data from the devices to determine if any abnormal data changes occur inside the greenhouse. Once an abnormal data change is identified, its location is determined, and path planning is performed to determine the maintenance path for that location. However, some abnormal data changes may not cause changes to the greenhouse environment or affect crop growth. Therefore, real-time operational data at the location of the abnormal data change is compared and analyzed to determine whether maintenance is necessary. This method not only ensures normal crop growth inside the greenhouse but also reduces greenhouse maintenance costs.

The application discloses a vector for editing Nicotiana benthamiana genes based on CRISPR / Cas9 and a construction method and application thereof, and comprises BKG-g10 and / or BKG-g11 vectors constructed for a UbEF1B gene of the Nicotiana benthamiana and / or BKG-g12 and / or BKG-g13 vectors constructed for a CCR4 / NOT gene of the Nicotiana benthamiana. After the UbEF1B gene or the CCR4 / NOT gene is edited by the vectors, the growth state of the Nicotiana benthamiana is normal, and the phenotype does not obviously change compared with a wild type; the vectors do not affect the traits of the Nicotiana benthamiana, and are beneficial to the continuation of offspring. Compared with a traditional breeding method, the CRISPR / Cas9 system can more quickly and effectively obtain disease-resistant materials by editing endogenous genes of the Nicotiana benthamiana.

The present application relates to the technical field of planting, in particular to a method for improving seed germination rate and leaf growth of passion fruit. The present application can improve the seed germination rate of passion fruit by soaking passion fruit seeds in ganoderma lucidum solution for a suitable time; by spraying ganoderma lucidum and foliar fertilizer on the leaves of passion fruit plants, the symptoms of viral diseases of the plants can be obviously improved, the use of pesticides can be reduced, the effect of reducing pesticide residues can be achieved, the passion fruit leaves can grow normally, and the leaf growth can be improved.

The utility model discloses a heterotrophic denitrification device and a heterotrophic denitrification system for deep denitrification of petrochemical high-salinity wastewater, aims to solve the problem of inhibition of a high-salinity environment on a heterotrophic denitrification nitrogen removal process, and provides an efficient and stable wastewater nitrogen removal solution. The device mainly comprises a salt-tolerant denitrifying flora immobilization system, a carbon source slow-release module and a reaction parameter monitoring system. The biological stability of the salt-tolerant heterotrophic denitrifying flora in a high-salt environment is improved through an immobilization technology, and sufficient nutrition supply is provided through a carbon source slow release system. The online monitoring system monitors key parameters such as pH value, dissolved oxygen, oxidation-reduction potential, salinity and pressure difference in real time to ensure optimal reaction conditions. Compared with the prior art, the device disclosed by the utility model can be used for efficiently and stably removing nitrogen pollutants in the wastewater in a high-salinity environment, is relatively low in operation cost and is widely applied to deep denitrification of high-salinity wastewater in industries such as petrochemical industry and the like.

The utility model belongs to the technical field of agriculture, and discloses an olive cutting seedling raising device which comprises a fixing assembly, an irrigation assembly is fixedly installed on one side of the fixing assembly, a culture assembly is arranged in an inner cavity of the fixing assembly and comprises an installation plate, a plurality of air bags are fixedly installed on one side of the installation plate, and the air bags are arranged in the inner cavity of the fixing assembly. The surface of the mounting plate is movably connected with a culture tank, an air pipe is connected between the air bags, and the end part of the air pipe is fixedly connected with an inflation box. Through the design of the irrigation assembly and the culture assembly, the air bags on the two sides of the culture groove are filled with air to bulge, and the two air bags clamp olive cutting branches, so that the olive cutting branches are fixed without damaging the cutting branches, and meanwhile, the olive cutting branches with different diameters can be fixed and clamped; inclination of the cutting branches during cultivation is avoided, normal growth of the whole root system is achieved, and the integrity of growth of the root system is guaranteed.

The invention provides a resistance marker-free auxotrophic bacillus subtilis and a construction method and application thereof, the newly constructed strain takes bacillus subtilis 168 as a starting strain, indole-3-glycerophosphate synthase gene trpC of the bacillus subtilis 168 is knocked out, synthesis of tryptophan in the growth and metabolism process of the strain 168 is eliminated, and the resistance marker-free auxotrophic bacillus subtilis is obtained. The shuttle vector YH46 is constructed by taking pMD-19T as a framework, so that the shuttle vector YH46 can normally grow only in the presence of exogenous tryptophan, and meanwhile, a universal auxotroph shuttle vector YH46 is constructed by taking pMD-19T as the framework, so that convenience is provided for high-efficiency expression of genes in bacillus subtilis; the tryptophan auxotrophic strain BS-TR is constructed through double exchange by using a homologous recombination principle, the construction process is simple and easy to implement, and the strain BS-TR does not contain a resistance gene selection marker, can be used as a food-grade engineering bacterium, and can be used for intracellular expression or extracellular secretion of target protein for production.

The present invention relates to a recombinant microorganism comprising a metabolic pathway for the synthesis of P34HB (poly-3-hydroxybutyric acid (3HB)-4-hydroxybutyric acid (4HB) ester) wherein the proportion of 4HB is increased by attenuating the synthesis of 3HB, thereby reducing the addition of precursor substances, achieving the purpose that the strain can grow normally and produce a high proportion of P34HB.