55results about How to "Normal growth" patented technology

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention relates to halophilous bacillus (Halophilous sp.) HMF09, and the preservation number of the halophilous bacillus is CGMCC (China General Microbiological Culture Collection Center) No.30475. The strain has high saline-alkaline tolerance and efficient cadmium ion immobilization capability, and can be applied to reduce the ion activity of heavy metal cadmium in soil, reduce stress of saline-alkaline on crops and promote crop growth.

The invention provides a high-salinity high-tidal-flat mangrove forest ecological restoration method. The high-salinity high-tidal-flat mangrove forest ecological restoration method comprises the following steps of S1, soil improvement, S2, seedling cultivation, S3, seedling planting, S4, seedling reseeding, S5, bank protection device arrangement and S6, pest control. The soil conditioner is used for improving the soil structure and reducing the soil salinity. Salt-resistant domestication is performed on the seedlings by gradually improving the salinity of irrigation water, so that the salt resistance of the seedlings is improved. Organic fertilizer, ferriporphyrin, abscisic acid and brassinolide are used for providing nutrients for seedlings, osmotic equilibrium in plants is adjusted, opening and closing of air holes are adjusted, plasticity of cell walls is adjusted, and therefore the salt resistance of the plants is enhanced. According to the method, the roots of seedlings are inoculated with the halorhodobryum terbach and the proteobacteria, the halorhodobryum terbach and the proteobacteria terbach form a symbiotic relationship with the roots of plants, a microbial community is formed, and the absorption efficiency of the plants to nutrients in soil is According to the method, the planting survival rate and the plant growth rate are improved through the multi-way overall synergistic effect, and then the biological diversity is improved.

The ecological slope protection structure for water and soil conservation for water conservancy construction comprises soil retaining plates fixedly supported on slopes on the two sides of a river, planting grooves for planting green plants are formed in the soil retaining plates, a water pumping pipe is fixedly connected, and the outer contour of the water pumping pipe is sleeved with a rectangular shell moving in a reciprocating mode; the rectangular shell is fixedly connected with a spray head and a drainage pipe which is in through connection with the spray head, in addition, a salvage mechanism and a liquid pumping mechanism are further arranged on the soil retaining plate and used for cleaning floating objects on the surface of river water and pumping the river water for irrigation, and the salvage mechanism achieves salvage of the floating objects through matched action of a cylindrical roller and a filter plate; according to the ecological slope protection structure, by means of an automatic irrigation system, the utilization efficiency of water resources is improved, the soil retention capacity is enhanced, plant growth is promoted, water and soil loss is effectively prevented, and the ecological slope protection structure can be applied to the field of ecological slope protection especially under the drought condition. The ecological environment is protected and recovered.

The invention relates to the technical field of biological genes, in particular to a rice herbicide-resistant OsEPSPS mutant gene, a protein and application thereof. The rice herbicide-resistant OsEPSPS mutant gene provided by the invention is characterized in that the 836th site of the nucleotide sequence of a wild rice OsEPSPS gene is mutated, and T is mutated into C. A plant containing the rice herbicide-resistant OsEPSPS mutant gene or the rice herbicide-resistant OsEPSPS mutant protein has remarkable resistance to glyphosate herbicide and can normally grow in the herbicide almost without being influenced, and under the same condition, the growth of a wild plant is remarkably inhibited until the wild plant dies, so that the rice herbicide-resistant OsEPSPS mutant gene or the rice herbicide-resistant OsEPSPS mutant protein has a good application prospect. The mutant gene and the mutant protein have huge market economic prospects.

The utility model discloses a rhododendron lapponicum seedling cultivation device, and mainly relates to the technical field of seedling cultivation. Comprising a seedling raising plate detachably arranged on a first bottom plate, a plurality of seedling raising cups are arranged on the seedling raising plate, a water storage chamber is formed among the first bottom plate, a second bottom plate and a seedling raising box, and a plurality of filter plates used in cooperation with the water storage chamber are arranged on the seedling raising cups; a plurality of atomizing nozzles used in cooperation with the seedling raising cups and spraying pipes communicated with the atomizing nozzles are arranged on the seedling raising box, a cover plate is arranged at the top of the seedling raising box, a shading plate is rotationally connected to the cover plate, and a drainage pipe communicated with the water storage cavity is arranged at the bottom of the seedling raising box. The Rhododendron lapponicum seedling raising device has the advantages that the problems that artificial seedling raising seeds are prone to loss, and seedlings are uneven and grow in a piled mode are solved, and the effect of planting Rhododendron lapponicum seedlings in batches is improved.

The invention discloses a pseudomonas strain and application thereof in kitchen waste deodorization, and belongs to the technical field of environmental microorganisms, the strain is classified and named as Pseudomonas sp., is preserved in China General Microbiological Culture Collection Center on April 15, 2024, has a preservation number of CGMCC No.30345, is preserved in No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and has a preservation number of CGMCC No.30345. According to the present invention, the Chinese Academy of Sciences Institute of Microbiology, the postcode is 100101, after the application, the strain can efficiently remove hydrogen sulfide, ammonia gas, VOCs and other components in the malodorous gas, and the new efficient and environmentally-friendly strain resource is provided for solving the air pollution problem caused during the garbage waste treatment process;

The agricultural water and fertilizer integrated irrigation device comprises a base and a water and fertilizer box fixedly connected to the top of the base, one side of the water and fertilizer box is fixedly connected with an infusion pump through a connecting block, and the water inlet end of the infusion pump communicates with a liquid inlet pipe. According to the agricultural water and fertilizer integrated irrigation device and method, a soil moisture sensor is inserted into soil to detect the moisture saturation, if the detected moisture value is lower than a moisture standard value, a central processing unit controls a liquid inlet pipe on a liquid pump to extract water and fertilizer from a water and fertilizer box, and the water and fertilizer are introduced into a drip irrigation pipe through a liquid outlet pipe; and if the detected moisture value is higher than a preset moisture standard value, the central processing unit controls the infusion pump to stop pumping water and fertilizer, so that the crops can be automatically irrigated, excessive or insufficient water and fertilizer irrigation is effectively prevented, the accuracy of water and fertilizer irrigation is improved, and the water and fertilizer irrigation efficiency is improved. And normal growth of crops is ensured.

The invention belongs to the field of plant tissue culture, and particularly relates to a culture medium composition and method for inducing plant regeneration through peony petioles, and the culture medium composition is characterized in that a callus induction tissue culture medium and an adventitious bud differentiation culture medium are MS culture mediums containing 0.5-1.5 mg / L TDZ and 0.05-0.25 mg / L NAA; the rooting induction culture medium is a 1 / 2 MS culture medium containing 2.0 to 4.0 mg / L of IBA, 0.3 to 0.7 mg / L of NAA and 0.4 to 0.6 g / L of TA. Petioles of peony sterile test-tube plantlets are used as explants; inoculating the explant into the callus induction culture medium for dedifferentiation culture to obtain a callus; inoculating the callus into the adventitious bud differentiation culture medium to induce bud differentiation to obtain adventitious buds; dividing the adventitious buds, and inoculating the adventitious buds into the rooting induction culture medium to induce rooting, so as to obtain regenerated seedlings. The culture medium is combined with a tissue culture method, petioles of peony seedlings are adopted for tissue culture, plant seedlings are obtained, the tissue culture seedling obtaining efficiency is high, the adventitious bud and adventitious root induction effect is good, and the transplanting survival rate is high.

The invention discloses application of a rice brown planthopper resistant gene OsCYT2 in rice brown planthopper resistance, and relates to the technical field of brown planthopper resistant transgenic rice. According to the invention, through agrobacterium-mediated genetic transformation, the OsCYT2 gene is transferred into an insect-susceptible rice variety Nipponbare, and the resistance of a plant over-expressed with the OsCYT2 gene to brown planthopper is enhanced. After the brown planthopper eats the transgenic plant, the survival rate of the brown planthopper is reduced, the weight gain of the brown planthopper is reduced, and the amount of secreted honeydew is reduced. The new insect-resistant function of the gene OsCYT2 found by the invention has reference significance for researching gene molecular functions and breeding.

The invention discloses a thorn-free Chinese prickly ash grafting device and a grafting method. The grafting device comprises a mounting plate, a grafting knife and a control structure, wherein two first sliding blocks are arranged on the bottom surface of the mounting plate; the grafting knives are correspondingly arranged at the bottoms of the first sliding blocks; the control structure comprises a support, a rotating shaft, a crank connecting rod assembly, two second sliding blocks and a gear, the second sliding blocks are arranged on the two sides of the surface of the mounting plate in parallel, each second sliding block is fixedly connected with one first sliding block, and the gear is rotationally arranged between the two second sliding blocks; gear teeth meshed with the gear are arranged on the second sliding blocks, the support is vertically arranged at the top of the mounting plate, the rotating shaft vertically penetrates through the upper side of the support and is rotationally connected with the support, one end of the crank connecting rod assembly is connected with the lower end of the rotating shaft, and the other end of the crank connecting rod assembly is fixedly connected with one second sliding block; therefore, the method can ensure that the final sections are consistent.

The utility model provides a disease and pest trapping device for wormwood planting, and belongs to the technical field of wormwood planting. The pest trapping device for wormwood planting comprises a pest trapping assembly and a pest killing assembly. The insect trapping assembly comprises a lampshade, an insect trapping lamp and a support, the insect killing assembly comprises a draught fan, a connecting cover, a blocking plate and a conductive net barrel, during use, the insect trapping lamp attracts winged insects to enter the lampshade, the draught fan sucks gas and the winged insects in the lampshade through an insect suction main pipe, and the winged insects enter the conductive net barrel under the action of the draught fan; winged insects can be killed by electricity when making contact with the conductive net cylinder, then insect killing is conducted, a plugging plate is pulled downwards, a connecting cover and the conductive net cylinder can be opened, the winged insects killed by electricity in the conductive net cylinder can fall out of the bottom end of the conductive net cylinder, then wormwood can be trapped and killed, the wormwood is not prone to being gnawed by the insects, on one hand, the wormwood can grow normally, and on the other hand, insect killing is conducted; on the other hand, the wormwood is not easy to get sick.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a genetic transformation method of perennial elymus nutans. According to the genetic transformation method of elymus nutans, mature embryos serve as explants for inducing callus formation, the method has the advantages that material obtaining is convenient, operation is simple and easy, the supply problem of explant material obtaining is solved, and meanwhile the physiological state of the mature embryos is consistent, so that the repeatability of experiments is good; in addition, the infection liquid contains the acetosyringone, and the acetosyringone (AS, Acetosyringone) can promote the growth of agrobacterium tumefaciens and enhance the activity of the agrobacterium tumefaciens, so that the conversion efficiency is improved; the browning phenomenon of the calluses is effectively inhibited through sterilization treatment, the normal growth of the calluses can be recovered, the transgenic elymus nutans plants are obtained through the technical scheme, and the research of researchers on the gene functions of the elymus nutans can be greatly promoted.

The utility model provides a movable floating bed device capable of reducing bottom mud pollution. The movable floating bed device comprises a floating bed, a waterproof enclosure, a stirring device and a fixed anchor block, the bottom area of the floating bed is enclosed into a closed area by the waterproof enclosure, so that the exchange between the internal water body and the external water body of the floating bed device is avoided during the operation of the floating bed device; the stirring device is a long-shaft stirring machine and is arranged at the geometric center position of the floating bed, the top of the stirring device is connected with the floating bed, and the stirring device is started to disturb sludge at the bottom of the water body, so that the water body in the waterproof enclosure forms turbid suspension liquid containing polluted organic substances. According to the floating bed device, the problem of poor growth of emergent aquatic plants in the floating bed in the micro-polluted water body is solved, meanwhile, water body bottom mud pollution can be degraded synchronously, and the requirements of purifying water quality and beautifying the environment are met.

The utility model provides a grape branch fixing buckle which comprises a locking box, a locking hole is formed in the locking box, a shifting piece is fixedly connected in the locking box, clamping teeth are arranged on the shifting piece, an annular belt is fixedly connected to one side of the locking box, oblique teeth are arranged at one end of the annular belt, and a sliding groove is formed in the annular belt. The grape branch locking device has the advantages that by arranging the sliding blocks, the connecting plates, the buffering boxes, the springs, the mounting plates, the connecting rods, the clamping plates and other structures, the ring belt is wound on a grape branch, the end, provided with the oblique teeth, of the ring belt is inserted into the locking box, and the oblique teeth at the proper positions are matched with the clamping teeth to lock the ring belt according to needs. The clamping plate is used for clamping and fixing the grape branches. When the grape branches grow thick, the clamping plates push the mounting plates through the connecting rods to compress the springs, grape paper slips are prevented from being clamped to form tightening marks, and the grape branches can grow normally. The grapes can be stably fixed, the growth of the grapes can be prevented from being limited, and the practicability is higher.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

The utility model provides a culture dish assembly which comprises a containing base body, the containing base body is in a cover shape with an upward opening, the top of the containing base body is provided with a plurality of culture units, each culture unit is in a cover shape with an upward opening, the bottom of each culture unit is provided with a root passing hole, and the root passing holes are communicated with the containing base body. And the root passing holes are communicated with the inner cavity of the accommodating base body. According to the culture dish assembly provided by the utility model, the culture effect is better.

The utility model provides a hydraulic engineering ecological wall which comprises a concrete foundation, the bottom of the concrete foundation is fixedly connected with a consolidated foundation, the top of the consolidated foundation is fixedly connected with a main wall body, the bottom of the concrete foundation is fixedly connected with a side wall, and filling grooves are formed in the two sides of the side wall. An ecological wall mechanism is fixedly installed on the side wall through the filling groove, the ecological wall mechanism comprises an anti-loss net, a soil layer, a coconut shred blanket, a gravel layer and a stone layer, the bottom of the anti-loss net is fixedly connected with the soil layer, the bottom of the soil layer is fixedly connected with the coconut shred blanket, the bottom of the coconut shred blanket is fixedly connected with the gravel layer, and the top of the gravel layer is fixedly connected with the stone layer; and through the anti-loss net and the coconut shred blanket, the maintenance cost of the ecological wall of the water conservancy project is reduced, and the problems that soft soil is easily scoured by river water, collapses and is easily scoured away are solved.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention relates to the technical field of soil saline-alkali soil improvement, and discloses a seasonal permafrost region soda saline-alkali soil improvement and crop cultivation method, which comprises the following steps: (1) digging a catchment lake, a deep ditch and a plurality of shallow ditches in saline-alkali soil to be improved, enabling the plurality of shallow ditches to be respectively connected with the deep ditch, enabling the deep ditch to be communicated with the catchment lake, and enabling the deep ditch to be communicated with the catchment lake; the deep ditch is communicated with the catchment lake, the depth L1 of the deep ditch meets the condition that L1 = h1 + delta h1 + h01, and the ratio of the depth of the deep ditch to the depth of the shallow ditch is 1.5-3.5; (2) digging at least one vertical shaft between two adjacent shallow trenches, wherein the ratio of the depth of the vertical shaft to the depth of the deep trench is 0.7-1.5; and (3) injecting water into the soil between the shallow ditches. According to the method, the soil structure can be improved, the soil acid-base property can be adjusted, the micro-ecological environment of the soil can be improved, and crops can normally grow in a good soil environment.

The utility model discloses a combined architectural design decoration flower stand with a sunshade component, comprising a transverse plate, both sides of the bottom end of the transverse plate are provided with support frames, one side of each support frame is provided with a placing plate, the inside of each placing plate is provided with a limiting mechanism, the top end of each placing plate is provided with a moving groove, and the top end of each moving groove is provided with a sunshade component. And clamping blocks are installed on the two sides of the top end of the containing plate correspondingly, fixing blocks are installed at the positions, on one sides of the clamping blocks, of the top end of the containing plate correspondingly, and water storage tanks are installed at the positions, on one sides of the supporting frames, of the bottom end of the containing plate correspondingly. According to the device, the driving shaft is screwed to drive the connecting block to do horizontal movement under the action of the moving block, the stored sunshade net is unfolded while the connecting block does horizontal movement, flower and green plants placed on the placing plate can be conveniently subjected to sunshade treatment through the operation, exposure of sunlight to the flower and green plants is reduced, and therefore the survival rate of the flower and green plants is increased; and the replacement cost is reduced.

This invention relates to a halophilic Bacillus ( Halobacillus sp HMF09, with accession number CGMCC No. 30475, is an antagonistic strain against *Fusarium oxysporum* tomato-specific strain. It exhibits high salt and alkali tolerance and efficient cadmium ion fixation. Application of this strain can reduce the ionic activity of cadmium in soil, alleviate salt and alkali stress on crops, and promote crop growth.

This utility model belongs to the field of land ecological environment restoration technology, specifically disclosing a land ecological environment restoration device, including a U-shaped plate. A fixing lug is fixedly connected to the outer wall of the U-shaped plate, and a fixing pin is inserted into the inside of the fixing lug. Arc-shaped surfaces are formed at both ends of the U-shaped plate, and diversion holes are formed inside the U-shaped plate. A water storage component is connected to the U-shaped plate to collect rainwater for subsequent irrigation of vegetation. By setting up the water storage component, the water storage box can store water through the diversion channel and water inlet during rain. After a certain period of time after the rain stops, the seal on the drip hole is opened, allowing the water inside the water storage box to be discharged and used to irrigate the vegetation. This achieves the effect of storing water during rain and subsequently irrigating the vegetation, preventing vegetation from drying out and dying, allowing the vegetation to grow normally, stabilizing the soil on slopes, and restoring the ecological environment of slopes.

The invention belongs to the technical field of plant cultivation, and particularly relates to an indoor optimal cultivation method and system for common bryophytes in alpine meadow, and the method comprises the following steps: collecting bryophyte samples; drying in a dry and dark place, and manually removing macroscopic impurities; crushing the dried moss sample into stem and leaf fragments with the length of 1-2mm by using a crusher to prepare an inoculation material, and uniformly mixing for later use; setting cultivation conditions; the nutrient soil subjected to high-temperature sterilization serves as a culture medium to be filled in a nutrition bowl with the caliber being 12 cm, 15 g of inoculation materials are scattered, and cultivation is conducted respectively; measuring the moss coverage and the plant density; measuring the thickness of the formed moss crust by using a vernier caliper, measuring the absorbance of the bryophyte under different wavelengths by using an ultraviolet spectrophotometer, and calculating the content of chlorophyll a and exopolysaccharide by using a formula. The bryophytes are cultivated indoors according to experimental design, the optimal cultivation conditions of the bryophytes can be obtained, data support can be provided for rapid propagation of the alpine meadow bryophytes, basic materials are provided for related research of the alpine meadow bryophytes, and the method has important significance.

The drip irrigation device for mango planting comprises an annular pipe, a connecting pipe, telescopic pipes, an inserting pipe and a connecting mechanism, the connecting mechanism comprises an inflating pump, an air conveying pipeline, an annular air bag, a control valve and an elastic collision bead, mango trees can be planted in the annular pipe, the telescopic pipes are arranged on the two sides of the annular pipe, and the inserting pipe is inserted into the annular pipe. The spacing between the annular pipes can be adjusted so as to meet different planting requirements, when irrigation water is conveyed to the annular pipes, the roots of mango trees can be uniformly irrigated through the irrigation holes in the bottoms, sufficient water supply is guaranteed, insertion pipes are arranged at the ends of the telescopic pipes, the insertion pipes are used for extending into the connecting pipes, and therefore the insertion pipes can be conveniently inserted into the connecting pipes. The elastic collision bead in the connecting pipe can be ejected into the positioning groove of the insertion pipe to realize detachable connection with the insertion pipe, so that when a certain part of the pipeline is damaged, the pipeline can be independently replaced, the planting cost is reduced, in addition, the blast pump can input air into the annular air bag, the outer wall of the insertion pipe is sealed, and irrigation water is prevented from overflowing.

The utility model discloses a prefabricated ecological crown beam which comprises a front vertical plate, a rear vertical plate is arranged on the rear side of the front vertical plate, and the front vertical plate and the rear vertical plate are arranged in a front-back mirror image mode. A cross beam is arranged between the front vertical plate and the rear vertical plate, and the front vertical plate, the rear vertical plate and the cross beam are connected through connecting bolts; the integral structure of the crown beam is split into single components of the plate and the beam, so that a mold structure is very simple during production, and the manufacturing cost of the mold is very low; the crown beam can be split into single components, so that the crown beam can be split in the production process, and after the crown beam is split into the single components for production, the operation process becomes simpler, and the production efficiency becomes higher; in addition, the storage space of the single assemblies is reduced, the storage capacity of the same area is increased, and the site occupation cost is reduced.

The invention relates to the technical field of ecological restoration, and discloses a natural reinforcement light supplementing system for water ecological restoration, an upper platform is fixedly mounted at the top of a floating platform through a stand column, a telescopic frame is arranged in the center of the bottom of the upper platform, and a plurality of telescopic light guide pipes are uniformly arranged between the top surface of the upper platform and the bottom surface of the telescopic frame; according to the device, the floating platform is arranged to be matched with the telescopic light guide pipe and the day-by-day light reflecting mechanism, so that aquatic plants planted in a deep water area can obtain good natural illumination, and the survival rate of the aquatic plants is effectively increased; the planting area of the submerged plants is effectively enlarged, the submerged plants can grow normally, water quality is improved, good basic conditions are provided for ecological environment treatment, the system is simple in structure and low in energy consumption, solar energy is utilized for power supply, illumination can be well provided for the aquatic plants at night, the survival rate of the aquatic plants is further increased, and the survival rate of the aquatic plants is increased. The water ecological restoration is facilitated.

The application discloses a deletion mutant of a rice LHCB5 gene and application thereof, and belongs to the field of gene editing. Through gene editing system, the 3'UTR region of the rice LHCB5 gene is precisely edited, and a 40bp homozygous deletion mutant of +16 to +55bp downstream of the termination codon TAA is obtained. The mutant can be stably inherited, and the growth phenotype is not significantly different from that of the wild type. Meanwhile, the resistance to rice blast and bacterial leaf blight is significantly improved, and the length of the disease spot is significantly reduced. The application first discloses the disease resistance regulation function of the 3'UTR of the LHCB5 gene, realizes the improvement of the disease resistance through the editing of the non-coding region, avoids the safety and agronomic trait risks brought by the transgene and the coding region editing, and the obtained mutant can be directly used for the breeding of the rice broad-spectrum disease resistance, and has important application value.

The invention discloses a DNA polymerase III mutant and application thereof. The mutant comprises an amino acid sequence as shown in SEQ ID No.1. The mutant can be applied to improvement of host bacterium genome mutation frequency. Escherichia coli is taken as a starting strain, and efficient evolution helper plasmids are introduced, so that the host mutation frequency is improved; and combining with the tryptophan structural analogue 5MT competitive pressure directional screening to finally obtain the escherichia coli engineering strain with high tryptophan yield. The helper plasmid comprises a pBad24 plasmid vector skeleton and an engineered escherichia coli DNA polymerase III danQ nucleotide fragment. The high-efficiency evolution helper plasmid provided by the invention can significantly improve the mutation rate of a host genome, can greatly shorten the traditional mutagenesis period by combining with 5MT directional screening, can efficiently screen high-yield tryptophan engineering strains, and has important application value.

The application discloses Pseudomonas songnenensis, a microbial inoculum and application thereof, and relates to the technical field of environmental microorganisms and solid waste resource technology. The Pseudomonas songnenensis strain JP01 is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No. 37102. The strain has salt and alkali tolerance characteristics, and can degrade coal gangue and promote the release of effective phosphorus, available potassium and effective silicon nutrients. After inoculation for 4 days, under normal conditions, the effective phosphorus content of the strain JP01 treatment group is 758.65% higher than that of the CK group; under salt and alkali conditions, the effective phosphorus content of the strain JP01 treatment group is 4.70 times that of the bacillus megaterium treatment group. The strain has good long-term colonization ability in a coal gangue-salt and alkali soil mixed substrate, and the viable cell count can still be maintained at a high level of 2.72x10 7 CFU / g after 90 days of culture. The application is suitable for coal gangue resource utilization and salt and alkali soil improvement, and has good phosphorus element activation ability and functional stability in a salt and alkali environment.