302results about How to "Improve insect resistance" patented technology

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses an organic fertilizer, which comprises the following raw materials including livestock excrement, poultry excrement, organic matters, domestic garbage, meal cake, leaves, rice husks, straw, weeds, crop straw, fire ashes, nitrogen fertilizers, phosphoric fertilizers, potash fertilizers, trace elements, compound bactericides 1 and compound bactericides 2. The invention also discloses a manufacture method of the microbial organic fertilizer. The microbial organic fertilizer disclosed by the invention has the advantages that the technical problems are solved to realize the effect that the household garbage, the leaves, the weeds and the like are perfectly applied to the organic fertilizer, and the defects that in the existing organic fertilizer product, phenomena of root burning and seedling burning more or less exist, the fertilizer effect and the effect period are equivalent to that of chemical compound fertilizers, the soil improvement effect is poor, and the like are overcome.

The present invention includes an improved electrostatic device for energizing fluids, in particular water based fluids, which will be used to provide a benefit to living organisms, machinery, processes and substances. The improved device of the invention will include an electrostatic voltage spike signal generator, two or more radio frequency signal generators, one or more antennas, optional one or more signal boosters and a fluid conduit. When fluid is treated with the improved device of the invention, the fluid will become energized and can be used to provide significant benefits in applications such as milk production, flower production, fruit production, crop production, vegetable production, shrimp production, egg production, meat production, gasoline combustion, waste fluid combustion, scale removal, water purification, fluid tracking, fluid sterilization and more.

The invention discloses an efficient multifunctional seaweed biological fertilizer and relates to the technical field of compound fertilizers. The raw materials include 10-80 parts of seaweed fermentation liquor, 5-30 parts of humic acid, 5-30 parts of urea, 5-20 parts of monoammonium phosphate, 5-20 parts of potassium sulfate and 2-10 parts of trace elements, and all the raw materials are mixed, concentrated and dried at low temperature to obtain the efficient multifunctional seaweed biological fertilizer. The efficient multifunctional seaweed biological fertilizer can quicken photosynthesis of crops, enhance disease resistance and drought resistance, improve crop yield and improve soil structure and soil water and nutrient retention capability, and is worth popularizing energetically.

The invention discloses a functional biological powder organic fertilizer or organic and inorganic fertilizer special or fruit trees and a preparation method thereof. The fertilizer is prepared by the following steps of: adding yeast into livestock excrement to perform anaerobic fermentation and aerobic fermentation (or biogas residue obtained by fermenting the livestock excrement through a biomass pool); and proportionally metering, crushing and mixing the fermentation product, a humic acid, a nitrogen fixing, phosphorus releasing and potassium releasing composite bacteria serving as an inoculant, a synergist, microelements necessary to plants and nitrogen, phosphorus and potassium inorganic nutrients by a computer. The product has the advantages of small using amount, quick response andlong duration; and the fertilizer is mainly used for basal application at the roots of the fruit trees to promote the strong growth of the fruit trees and quicken the transformation and decompositionof original substances of the soil so as to improve the soil fertility level. The fertilizer pelletizes and loosens the soil, improves the water-retaining property and air permeability, slowly releases the nutritive elements necessary to the fruit trees, enhances the nutrient absorption of the fruit trees, promotes the root development, has cold resistance and drought resistance, prevents waterlogging, resists diseases, protects flowers and fruits, improves the quality and prolongs the preserving period of the fruits. The product of the invention plays an important role in recycling waste resources and protecting the ecological environment.

The present disclosure provides formulations of pyrethroid compounds comprising nanoparticles of polymer-associated pyrethroid compounds along with various formulating agents. The present disclosure also provides methods for producing and using these formulations. The disclosure describes various formulations and formulating agents that can be included in the formulations. Additionally, the disclosures describes application to various plants and pests as well as advantages of the disclosed formulations.

The invention discloses a functional liquid bioorganic fertilizer and a preparation method thereof. The preparation method comprises the following steps of: fermenting livestock manures to form the mixture of biogas slurry and biogas residues, separating the biogas residues to obtain clear liquid, decomposing the clear liquid, adding photosynthetic bacteria into the clear liquid for intermediate culture, and mixing the cultured clear liquid, chitosan, a synergist, and trace elements required by plants in proportion to prepare the functional liquid bioorganic fertilizer. The functional liquid bioorganic fertilizer has a plurality of functions of promoting the transformation of soil substances, improving soil structure, improving the fertility of soil, promoting the growth of crops, fixing nitrogen, improving the nitrogen level of the soil, promoting the multiplication of beneficial microorganisms of the soil, participating jointly in the circulation of substances of soil ecosystem, improving the cold resistance, drought resistance and disease resistance of the crops, and the like, has the characteristics of small using amount, quick response and high effect persistence so as to reduce the using amounts of chemical fertilizers and pesticides, reduce agricultural pollution and save agricultural cost, can be mainly used for foliage dressing and as a base fertilizer as well as a root fertilizer, plays a very important role in recycling finite resources and protecting the agroecological environment, is particularly applied to the production of organic green pollution-free agricultural products so as to improve the quality and yield of the organic agricultural products, and is of great significance for the realization of ecocycle economy in China.

The invention discloses an excretion fermented seedling compound fertilizer. The compound fertilizer is prepared from the following raw materials in parts by weight: 500-600 parts of livestock excretion, 120-140 parts of water hyacinth powder, 100-120 parts of castor stems, 100-120 parts of silkworm excrement, 100-120 parts of corn stalk, 80-90 parts of cottonseed cake, 40-50 parts of tobacco stems, 30-40 parts of pulverized fuel ash, 12-15 parts of river sand, 20-25 parts of calcium superphosphate, 15-20 parts of urea, 8-12 parts of potassium chloride, 1-2 parts of compound sodium nitrophenolate, 2-3 parts of copper sulfate, 6-9 parts of EM (effective microorganism) bactericide, 5-7 parts of microbial starter, 10-15 parts of soil conditioner, and a proper amount of water. The fertilizer adopts livestock excretion and the like as main raw materials through microbial fermentation, is rich in organic fertilizer, inorganic fertilizer and various nutrition, does not have toxic or side effect, does not pollute environment, can remarkably supply nutrition required by seedlings, can improve the soil structure, promote growth and development of seedlings, and improve the resilience of disease resistance, insect resistance, drought resistance, water logging resistance, cold resistance, salt alkali resistance, lodging resistance and the like.

The invention discloses a method for cultivating a transgenic plant with improved insect resistance by using RNA interference technology and a special DNA fragment thereof. The DNA fragment is a gene fragment shown as a formula I, wherein a forward sequence (SEQ) is any one fragment which at least comprises 19bp in full-length cDNA fragments of an ATP synthase E subunit gene; a reverse sequence (SEQ) and the forward sequence (SEQ) are mutually reversely complementary; X is a spacer sequence between the forward sequence (SEQ) and the reverse sequence (SEQ), and the X and the forward sequence (SEQ) or the X and the reverse sequence (SEQ) are both not complementary mutually; and the full-length cDNA of the ATP synthase E subunit gene is shown as a sequence 1 in a sequence table. The invention also aims to provide application of the ATP synthase E subunit gene serving as an RNA interference target gene in cultivating the transgenic plant with improved insect resistance. Experiments prove that aphids inoculated to wild-type tobacco grow and reproduce normally; and aphids inoculated to transgenic tobacco starts to die on the fourth day, dead aphid bodies is blackened after five days and no live aphids can be seen after seven days. The formula (I) is forward sequence (SEQ)-X- reverse sequence (SEQ).

The invention relates to a method used for preparing a drip-irrigation special-purpose fertilizer from biogas slurry. The method comprises following steps: (1) biogas slurry obtained via more than one month of normal and stable gas generation is pumped into a buffer tank for natural sedimentation so as to obtain a supernate; (2) the supernate is filtered so as to obtain a filtered biogas slurry, the filtered biogas slurry is sterilized and is pumped into a nutrient element adding tank and a microorganism adjusting tank respectively; (3) potassium humate, urea powder, monopotassium phosphate, monoammonium phosphate, organic silicone, and trace elements are added into the nutrient element adding tank successively, and are stirred until dissolving is realized so as to obtain a biogas slurry containing nutrient element solutions; (4) a microbial agent is added into the microorganism adjusting tank, and an obtained mixture is uniformly stirred so as to obtain a biogas slurry containing microorganism; and (5) the biogas slurry containing nutrient element solutions and the biogas slurry containing microorganism are delivered into a composite microorganism fertilizer stock solution tank for stirring so as to obtain a fertilizer stock solution, and the fertilizer stock solution is filtered so as to obtain a clear solution which is the drip-irrigation special-purpose fertilizer. Operation steps of the method are simple; production cost is low; and the method is convenient.

The invention relates to a calcium-magnesium-containing organic mineral fertilizer. The calcium-magnesium-containing organic mineral fertilizer consists of the following ingredients in percentage by weight: 5 to 30 percent of lime nitrogen, 10 to 15 percent of calcium magnesium phosphate fertilizer, 20 to 25 percent of turf, 10 to 15 percent of plant ash, 25 to 30 percent of dolomite powder with granular size of less than or equal to 60 meshes and 5 to 10 percent of bentonite. The preparation method comprises the following steps of: weighing the lime nitrogen, the calcium magnesium phosphate fertilizer, the turf, the plant ash, the bentonite and the dolomite powder according to the weight ratio; placing the materials into a stirring machine to stir and mix; performing pelleting by using apelletizer; roasting the materials at the temperature of between 200 and 400 DEG C for 20 to 50 minutes by using a roaster; and sieving the materials by using a sieving machine to prepare the calcium-magnesium-containing organic mineral fertilizer. By the calcium-magnesium-containing organic mineral fertilizer and the preparation method thereof, yield increment of crops can be promoted; quality of the crops can be improved; the structure of soil can be improved; and the crops are safe to eat.

The invention disclosed a compound fertilizer which comprises basic nutrition fertilizer including nitrogen, phosphorus and potassium, and also comprises gamma-polyglutamic acid and seaweed extract, wherein the gamma-polyglutamic acid accounts for 0.1-2% of the basic nutrition fertilizer, and the seaweed extract accounts for 0.5-2% of the basic nutrition fertilizer. The compound fertilizer containing the gamma-polyglutamic acid and the seaweed extract has high activity biological function, and is capable of improving the yield and the resistance capability such as disease resistance, drought resistance, cold resistance, lodging resistance and the like of crops. Therefore, the compound fertilizer can be widely applied to grain crops such as wheat, rice, corn and the like, and cash crops such as tomatoes, melons, grapes and the like. One-off fertilization can be realized without topdressing, and the yield of the crops can be increased by 10-30%.