20 results about "Microbial ecosystem" patented technology

The invention relates to the field of agricultural soil improvement and ecological fertilization, and discloses an ecological fertilization method for improving the organic matter content of soil in a dry-hot valley region, and the method comprises the following steps: soil matrix improvement: improving the porosity and water binding capacity of the soil by adding charcoal and humic acid particles; the functional microbial preparation is put, and the activity of soil microorganisms is activated through the combined effect of cellulose decomposition bacteria, stabilization promoting flora and water retention flora; green manure crop planting and covering management: planting sweet clover and other leguminous crops to provide a carbon source and a nitrogen source for soil, and meanwhile, utilizing straw covering to inhibit water evaporation; turning over and stabilizing the green manure crops, namely turning over and stabilizing the organic matters in a blooming period of the green manure crops and combining with a stabilization promoting bacterial liquid and an adsorptive carrier; dynamic balance is maintained, and dynamic balance of decomposition, conversion and accumulation of organic matter is achieved step by step through multi-cycle planting and turning and pressing. The organic matter content and fertility of the soil can be improved, and the water-retaining property and microbial ecosystem of the soil are improved.

The present invention relates to the technical field of feeds and particularly relates to a piglet weaning transition feed containing lactic acid bacteria and saccharomycetes compound fermented soybean meal and a preparation method thereof, and the piglet weaning transition feed comprises the following components in parts by weight: 15-20 parts of compound fermented soybean meal, 45-50 parts of corn, 10-15 parts of wheat, 5-7 parts of fish meal, 3-5 parts of whey powder, 3-4 parts of vegetable oil, 1-2 parts of an amino acid supplement and 2-3 parts of a mineral premix, 1-2 parts of a vitamin premix and 1.5-2 parts of a calcium-phosphorus supplement; wherein the compound fermented soybean meal is prepared by performing compound fermentation on soybean meal through lactic acid bacteria and saccharomycetes, and a complementary and symbiotic microbial ecological system is formed by carefully selecting and optimizing the combination proportion of three lactic acid bacteria and two saccharomycetes, so that the fermentation efficiency and stability are improved, and the functionality of a fermentation product is enhanced. Different strains complement each other in the aspects of carbohydrate metabolism, pH tolerance and functional characteristics, and nutrition competition is reduced.

The invention relates to methods and compositions for preventing microbial infections. Methods and compositions for permanently affecting the microbial ecosystem (microbiome) in a subject to prevent infection and reduce recurrence of microbial infections are provided. In some embodiments, compositions and methods are provided for the production and use of molecularly modified bacterial strains having potential to prevent infection with a variety of microorganisms.

The invention relates to the technical field of agricultural microorganisms, in particular to paenibacillus brevis KF-B6 and application thereof, in particular to application in prevention and treatment of continuous cropping obstacles of apples. The preservation number of the paenibacillus brevis KF-B6 is CGMCC (China General Microbiological Culture Collection Center) No.32131. The paenibacillus brevis KF-B6 has the compound functions of quickly producing EPS (Expandable Polystyrene) at high yield, producing chitinase at high yield, producing water-soluble and volatile antibacterial substances, decomposing macromolecular organic matters and the like. The application of the bacterium, the microbial agent and the compound microbial fertilizer thereof can effectively improve the number of bacteria in rhizosphere soil, reduce the number of pathogenic fungi, increase the proportion of the bacteria to the fungi and promote the restoration of a soil microbial ecosystem of a continuous cropping obstacle apple orchard; the proportion of water-stable aggregates is increased, the soil organic matter content is increased, the soil enzyme activity is improved, and the soil is improved; the survival rate of planted saplings in the new variety updating process of the continuous cropping obstacle old orchard is increased, the plant root activity and plant growth are promoted, and the apple continuous cropping obstacle is comprehensively relieved and prevented.

The invention discloses a microflora abundance prediction method based on center logarithmic ratio transformation, and belongs to the technical field of microflora prediction. Component characteristics of microorganism abundance data are processed through center logarithmic ratio transformation, and systematic preprocessing is carried out on input characteristics in combination with unique seasonal periodic coding. An enhanced Transform prediction model specially designed for table data is constructed, the model explicitly learns the cross relation between features through a novel feature interaction layer, and nonlinear mapping is carried out in combination with a multi-head self-attention mechanism and a deep network structure. By adopting the method, deep learning is carried out on the microbial community modeling data and the environmental factors, the composition abundance of the target microbial community can be efficiently and accurately predicted, and the technical problem that a traditional statistical model is difficult to capture a complex nonlinear relationship and high-dimensional feature interaction is solved; therefore, a strong technical support is provided for dynamic analysis and accurate regulation and control of a microbial ecological system.

The invention relates to the technical field of environmental engineering, and discloses a sewage filtering method and system based on multi-crop collaboration.The sewage filtering method based on multi-crop collaboration.The sewage filtering method based on multi-crop collaborationcomprises the steps that an original sewage sample is obtained, a multi-factor comprehensive evaluation method is adopted for analysis and treatment, and a multi-crop collaborationpollutant removal mechanism model is established; a multi-level system structure is constructed, and a three-dimensional hydraulic modeling method is adopted for analysis and calculation; a multi-sensor fusion monitoring network is constructed, and a data fusion algorithm is adopted for processing and analysis; system parameters are optimized and adjusted by adopting an intelligent self-adaptive control strategy; regularly adding a microbial agent to carry out system biological enhancement and dynamic regulation and control optimization, and establishing a stable microbial ecological system; all functional units are integrated into a modular system, and performance verification is carried out based on a stable microbial ecological system to obtain purified water; the system has the advantages of being high in treatment efficiency, stable in operation, high in intelligent level and low in construction cost.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention comprises a defined and safe soil substitute, herein referred to as “deconstructed soil”, that supports ecological balance in anaerobic microbial ecosystems, such as that inside the human gut, by shifting energy availability to favor growth of bacteria linked with gut health, and away from the most dominant species and species that are putatively harmful. The invention enables vulnerable bacterial groups to recover from a state of apparent extinction from the ecosystem in question. As it does not contain components that are prebiotic or probiotic, the deconstructed soil represents a new product concept for preventing and treating conditions associated with dysfunctional microbial ecosystems in the gastrointestinal tract of humans and animals, as well as other anaerobic microbial ecosystems.

The invention relates to the technical field of shield tunnel construction, solves the technical problem of hysteresis environmental risk of an ecological sensitive area caused by lack of an effective assessment mechanism for hidden damage to an underground microbial ecosystem in a shield tunneling process, and particularly relates to an assessment method and system for a shield tunneling process. The method comprises the following steps: collecting geological data and microbial data of an ecological sensitive area to construct a microbial distribution heat map; tunneling parameters of the shield tunneling machine are captured in real time, and vibration environment self-adaptive ecological response data collection and correction are synchronously executed in the shield tunneling process. According to the method, a quantitative reference is established by collecting geological data and microorganism data, tunneling parameters and ecological response data are dynamically monitored, a scientific reference system is provided for evaluating ecological disturbance caused by shield tunneling, and real-time monitoring is achieved.

The present invention discloses a targeted editing system of the hdrE gene of Methanosarcina and an in vitro evaluation method and application of its effect on bovine rumen methane production, relating to the field of gene editing technology, comprising the following steps: constructing a targeted editing system of the hdrE gene of Methanosarcina; mixing the targeted editing system of the hdrE gene of Methanosarcina with bovine rumen fluid, silage, and conversion mixture to form an in vitro fermentation system; culturing the fermentation system under anaerobic conditions at 39°C; and detecting at least one of the following indicators: relative abundance of Methanosarcina, methane gas production, and changes in rumen microbial community structure. The present invention comprehensively and deeply evaluates the effect of the targeted editing system of the hdrE gene of Methanosarcina on bovine rumen methane production from multiple dimensions, including gene editing effect, changes in the number of target microorganisms, and the entire rumen microbial ecosystem.

The invention relates to a flora regulation and control effect determination method, device and equipment, a medium and a program product. The method comprises the following steps: determining a target intestinal flora from to-be-selected intestinal flora, determining a target traditional Chinese medicine component from to-be-selected traditional Chinese medicine components according to the target intestinal flora and to-be-selected traditional Chinese medicine components, and then constructing an in-vitro model for simulating a human intestinal physiological environment based on a human intestinal microbial ecosystem simulator SHIME. A control experiment is performed based on the in-vitro model, the target intestinal flora and the target traditional Chinese medicine components, so that the regulation effect of the target traditional Chinese medicine components on the target intestinal flora can be determined based on data of the control experiment, and a mechanism of regulating intestinal immunity by traditional Chinese medicine is deeply disclosed. Moreover, according to the embodiment of the invention, the human intestinal physiological environment is simulated based on SHIME, the in-vitro model closer to the real physiological state is provided, and the accuracy of the regulation effect obtained based on the in-vitro model is further improved.

The present invention discloses a sewage treatment process and device based on synergy, and relates to the technical field of sewage treatment. In order to solve the problem of treatment stability, the process is realized by the synergy of artificial fillers and plant roots. The device includes a treatment pool, a planting bed is arranged inside the treatment pool, a planting bed is arranged on the bottom inner wall of the treatment pool, and a water outlet is arranged at the top and bottom of one side of the treatment pool, respectively, and a water inlet is arranged at the bottom of the other side of the treatment pool. A valve control component is connected to the outside of the water inlet, and the space between the aeration pipe and the planting bed of the treatment pool is provided with artificial fillers. The present invention arranges artificial fillers in the suspended growth of artificial fillers, and uses natural plant roots to replace part of the artificial fillers, so as to improve the biodiversity in the treatment pool, build a good microbial ecosystem, maintain benign growth and circulation, and ensure the stable treatment capacity of biological treatment.

The invention relates to the technical field of tiger fish culture, in particular to an ecological circulating water culture method for tiger fish, which comprises the following steps: cleaning a culture system; a system water culture stage; training to eat artificial feed, transferring the fry into the culture pond, and feeding brine shrimps in the first two weeks; after the state of the fry is stable, water flow disturbance is formed through a micro water spraying device at one corner of the culture pond, artificial feed is put on the disturbed water flow, and the fry begin to ingest; a screening culture stage: carrying out screening culture on the fries once after the fries are cultured for one month, putting the fries with similar specifications into the same culture pond, and ensuring the relative balance of the specifications of the fries in each culture pond; and a cultivation stage. According to the method, the Indonesia tiger fish is cultured by adopting ecological circulating water, the water quality can be stabilized by adopting a mode of a microbial ecological system, an electrochemical technology and circulating water culture, harmful bacteria and parasitic diseases can be prevented and controlled, and the standard-reaching rate and the survival rate of the tiger fish are greatly improved.

The invention relates to the technical field of wine brewing, and provides an artificial pit mud wet culture method. The method comprises the following steps: S1, collecting a sample from old pit mud, and separating to obtain an anaerobic strain; s2, respectively carrying out activation culture and adaptive pre-culture on the separated strains; s3, preparing a pit mud matrix, and performing nutrition activation treatment; s4, uniformly planting the activated mixed flora on the substrate, and slightly stirring to ensure uniform distribution; s5, dynamically controlling fermentation in stages in the fermentation process until the pit mud reaches a mature state; and S6, performing real-time monitoring and component detection on the fermentation process. Core microorganisms such as anaerobic bacillus, lactic acid bacteria and methane-oxidizing bacteria are separated and activated from the aged pit mud, the technical effect of rapidly establishing a high-activity flora ecosystem is achieved through the technical scheme of activation culture and adaptive pre-culture, the defects that the flora colonization speed is low, and the microbial ecosystem is unstable are overcome, and the method is suitable for large-scale popularization and application. The mature period of the new pit mud is obviously shortened.

The invention relates to the field of prevention and treatment of ancient plants, and particularly discloses a method for disease prevention and rejuvenation after transplantation of ancient and famous trees. The method comprises the following steps: taking tissues and rhizosphere soil of diseased plants of ancient and famous trees, sequencing amplification products after extracting DNA (Deoxyribose Nucleic Acid), counting the types and quantity of OTUs in the diseased plants and the rhizosphere soil, analyzing the composition and diversity of microbial flora, and determining growth critical values of harmful pathogenic bacteria and induced pathogenic bacteria; and according to the growth condition of the microbial flora, sequentially performing macro-environment physical measure prevention and control to improve the growth environment of diseased plants, chemical prevention and control to inhibit harmful bacteria, and biological prevention and control to form a beneficial flora effect. By adopting the method, diseases and pests of the ancient peonies can be effectively prevented and treated, a benign microbial ecological system is established around the transplanted plants, and the autoimmunity of the plants is enhanced, so that the purposes of comprehensively preventing and treating diseases and promoting rejuvenation of the ancient peonies are achieved.

The invention discloses a faeces water quality decomposing agent and a culture process thereof. The faeces water quality decomposing agent is prepared from the following components in parts by weight: 15 to 30 parts of photosynthetic bacteria, 10 to 20 parts of pseudogreen photosynthetic bacteria, 3.75 to 7.5 parts of bacillus amyloliquefaciens, 5 to 10 parts of proteolytic enzyme, 2 to 4 parts of humic acid, 0.5 to 1 part of sodium alginate and 1 to 2 parts of monopotassium phosphate. By optimizing the strain combination and auxiliary component proportion, an efficient and synergistic microbial ecological system is formed, various organic matters in the excrement can be quickly decomposed, and the organic fertilizer is suitable for wide organic waste treatment of human, livestock and poultry excrement and the like; a pure biological technology is adopted, the method is environment-friendly and pollution-free, and decomposed products can be used as high-quality organic fertilizers to realize resource circulation; the culture process is scientific and reasonable, parameters of each step are clear, links from strain activation to enzyme preparation and the like are all optimized, and the quality and stability of the decomposer are improved; the raw materials are wide in source and low in price, the culture process is simple, and the treatment cost is greatly reduced.

The application discloses an ecological additive for rock slope repair and a preparation method thereof, and belongs to the technical field of ecological restoration; the application aims to solve the problems of plant growth inhibition and community degradation caused by cement strong alkalinity and structure hardening in the existing vegetation concrete technology; the ecological additive is prepared by compounding microbial inoculants, mineral components, soil conditioners, structure improvers and organic carriers in a specific ratio, and the preparation method comprises microbial propagation composting and mixing; the additive is mixed with planting soil, cement, rice husks and the like in a certain ratio to prepare vegetation concrete base materials for spray seeding; the additive can neutralize the alkalinity of cement, improve the group structure of the base materials, construct an active soil microbial ecological system, and provide nutrients, so that the seed germination rate, vegetation coverage, soil organic matter content and plant biomass are improved, and the raw material cost is low, the process is simple, and the additive is suitable for large-scale ecological restoration engineering of high and steep rock slopes.

The invention belongs to the technical field of water environment restoration, and particularly relates to a slow-release composite algistat with algal inhibition and phosphorus removal functions and a using method and application thereof.The slow-release composite algistat is composed of an active algistat component, a polymer carrier, a phosphorus removal active component and a particle dispersing agent, the mass volume concentration of the active algistat component is 20-100 g / L, and the mass volume concentration of the polymer carrier is 20-100 g / L; the mass volume concentration of the polymer carrier is 5-50 g / L, the mass volume concentration of the phosphorus removal active component is 5-100 g / L, and the mass volume concentration of the particle dispersant is 2-20 g / L; according to the eco-friendly long-acting slow-release compound algistat and the scene adding method thereof, the efficient and more than 80-day long-acting treatment and prevention of blue-green algae for water bodies with different seasons and pollution degrees can be realized, and the ecological environment-friendly long-acting slow-release compound algistat has a wide application prospect. Meanwhile, the method has the outstanding advantages of reducing secondary pollution of nitrogen and phosphorus and promoting restoration of a microbial ecological system, is high in engineering applicability and has wide popularization and application prospects.

The invention discloses a method for rapidly producing an organic fertilizer from organic wastes by a strain ecological method and a microbial composition used in the method, and belongs to the technical field of organic waste treatment. The method aims to solve the problems of serious nitrogen volatilization loss, low functional flora synergistic efficiency and insufficient product decomposition degree in the fermentation process in the prior art. According to the method, a multi-component product is adopted and comprises a signal slow release type functional carrier (composition A) loaded with microbial population sensing signal molecules, a hydrolysis starting period fungicide (composition B), a signal response type thermophilic fungicide (composition C) and a decomposition synergistic period fungicide (composition D). Firstly, the composition A is mixed with organic waste, and then the compositions B, C and D are added in a sequencing batch mode according to the stage sequence of hydrolysis, high temperature and decomposition. According to the method disclosed by the invention, a microbial ecological system combining signal regulation and time sequence inoculation is constructed, so that the nitrogen loss is remarkably reduced, the quality and the decomposition degree of the organic fertilizer are improved, and the fermentation period is shortened.

Methods and compositions are provided for durably influencing microbiological ecosystems (microbiomes) in a subject in order to prevent infection and reduce recurrence of infection by microorganisms. In some embodiments, compositions and methods are provided for the creation and use of molecularly-modified bacterial strains with the potential to prevent a variety of microorganism infections.