Ecological recirculating aquaculture method for tiger fish

Abstract

The invention relates to the technical field of tiger fish culture, in particular to an ecological circulating water culture method for tiger fish, which comprises the following steps: cleaning a culture system; a system water culture stage; training to eat artificial feed, transferring the fry into the culture pond, and feeding brine shrimps in the first two weeks; after the state of the fry is stable, water flow disturbance is formed through a micro water spraying device at one corner of the culture pond, artificial feed is put on the disturbed water flow, and the fry begin to ingest; a screening culture stage: carrying out screening culture on the fries once after the fries are cultured for one month, putting the fries with similar specifications into the same culture pond, and ensuring the relative balance of the specifications of the fries in each culture pond; and a cultivation stage. According to the method, the Indonesia tiger fish is cultured by adopting ecological circulating water, the water quality can be stabilized by adopting a mode of a microbial ecological system, an electrochemical technology and circulating water culture, harmful bacteria and parasitic diseases can be prevented and controlled, and the standard-reaching rate and the survival rate of the tiger fish are greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of tiger fish breeding, and in particular to an ecological circulating water breeding method for tiger fish. Background Art

[0002] The Indonesian tiger fish (Greecepis truncatus) is native to Southeast Asia, primarily distributed in freshwater basins in Sumatra and Kalimantan, Indonesia, inhabiting rivers, lakes, and swamps. It is a valuable freshwater ornamental fish, typically kept in glass aquariums and often used as a companion fish for arowana and other species. It can live up to 10-15 years in captivity and is highly sought after. However, centralized, large-scale tiger fish farming requires frequent water changes to ensure their growth, resulting in significant waste of water resources. Furthermore, the long-term use of antibiotics to prevent and control fish diseases severely impacts the health of the fish. Therefore, a method for ecologically recirculating aquaculture of tiger fish (Greecepis truncatus) is proposed. Summary of the Invention

[0003] In response to the shortcomings of the existing technology, the present invention provides an ecological recirculating aquaculture method for tiger fish. Through the model of microbial ecosystem + electrochemical technology + recirculating aquaculture, it can not only stabilize the water quality, but also play a role in preventing and controlling harmful bacteria and parasitic diseases, greatly improving the compliance rate and survival rate of tiger fish.

[0004] To achieve the above object, the present invention provides the following technical solution: an ecological circulating water culture method for tiger fish, comprising the following steps: (1) Cleaning of the aquaculture system: Clean and air-dry the water storage tank, aquaculture tank, primary reaction tank, secondary reaction tank and balance tank; (2) System water cultivation stage: Add tap water to the system and keep it in operation; add urea and brown sugar to the secondary reaction tank, supplement with aeration and water cultivation, and add composite bacteria at the same time; add mixed algae to the culture pond every two days for one month, and then change to once a week; (3) Training the fry to eat artificial feed: Transfer the fry to the breeding pond and feed them with brine shrimp for the first two weeks. After the fry are stable, use a micro-spray device in one corner of the breeding pond to create water disturbance and place artificial feed on the disturbed water flow. As the water flow is disturbed, the fry will gradually start to eat. Feed them 4 times a day for a total of 10-15 days to complete the transition from biological bait to artificial feed. (4) Screening and breeding stage: every month of breeding, the fry are screened and bred once, and the fry of similar size are put into the same breeding pond to ensure the relative balance of the fry size in each breeding pond. The fry are screened 4 times in total and then transferred to the next level of breeding; (5) Growing stage: The healthy fry that are screened and transferred to the breeding pond are cultured continuously for 6 months, and lactic acid bacteria are fed once a week during this period.

[0005] Preferably, in step (2), the amount of urea added is 0.01 g / L, and the amount of brown sugar added is 0.1 g / L.

[0006] Preferably, in step (2), the composite bacteria is a strain composed of ammonia-forming bacteria, nitrifying bacteria and denitrifying bacteria in a mass ratio of 2:2:1, and the amount of the composite bacteria added to the secondary reaction tank is 0.2-0.5 g / L.

[0007] Preferably, in step (2), the mixed algae is a mixture of Chlorella vulgaris, Creeping algae and Algae in a mass ratio of 2:1:1, and the algae density is 1×10 6 cfu / mL, and the single release amount in the culture pond is 50L.

[0008] Preferably, in step (3), the method for preparing the artificial feed is as follows: weigh the following raw materials in percentage: 15-20% of whiteleg shrimp meat, 35-40% of fish meal, 8% of soybean protein concentrate, 10-15% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals, 0.4% of complex vitamins, and 5-10% of complex probiotic fermentation sterilization liquid; stir the above raw materials evenly, extrude into granules, and dry them to prepare the artificial feed.

[0009] Preferably, in terms of mass percentage, the composite mineral consists of 30% calcium lactate, 6% ferrous sulfate, 0.2% copper methionine, 0.25% chromium methionine, 3% zinc methionine, 0.05% sodium selenite, 0.2% magnesium sulfate, 0.8% calcium iodate, and 59.5% zeolite powder; in terms of mass percentage, the composite vitamin consists of 9% vitamin A acetate, 2.5% vitamin D3, 20% vitamin E, 6% vitamin B1, 5% vitamin B2, 4% vitamin B6, 1.5% vitamin K3, 0.6% biotin, 0.5% folic acid, 11% inositol, and 39.9% corn gluten meal; and the composite probiotic fermentation sterilization liquid consists of lactic acid bacteria and yeast in a mass ratio of 1:1.

[0010] Preferably, in step (3), after each feeding, the uneaten artificial feed in the breeding pond is promptly cleaned out.

[0011] Preferably, in step (4), during the screening and breeding stage, artificial feed is fed 4 times a day, with the total daily feeding amount accounting for 1-2% of the total weight of the fry, the water temperature of the breeding pond is 28°C, and the dissolved oxygen is above 5 mg / L.

[0012] Preferably, in step (4), feeding of artificial feed is stopped the day before each screening.

[0013] Preferably, in step (5), during the growth stage, artificial feed is fed three times a day, with the total daily feeding amount accounting for 1-2% of the total weight of the fry, the water temperature of the culture pond is 28°C, and the dissolved oxygen is above 5 mg / L; based on the artificial feed feeding amount, the lactic acid bacteria feeding amount is 2 g / kg.

[0014] The present invention provides an ecological circulating aquaculture method for tiger fish, which has the following beneficial effects compared with the existing technology: The present invention adopts ecological circulating water to culture Indonesian tiger fish. Through the model of microbial ecosystem + electrochemical technology + circulating water culture, it can not only stabilize the water quality, but also play a role in preventing and controlling harmful bacteria and parasitic diseases, greatly improving the compliance rate and survival rate of tiger fish.

[0015] The present invention conducts feeding training management on the initial fry transported, and realizes the transformation from biological bait to artificial feed. On the one hand, it can reduce the disease problem caused by feeding frozen bait in the later stage, and on the other hand, it can better meet the needs of large-scale breeding management, which is conducive to the healthy growth of tiger fish.

[0016] The present invention utilizes a micro water spray device to simulate dynamic water feeding, which puts the feed in a moving state. This is in line with the feeding habits of tiger fish that prefer fresh bait, allowing the tiger fish to eat better. At the same time, it can increase the amount of exercise of the tiger fish and improve its physical fitness, thereby increasing the compliance rate and survival rate of the tiger fish.

[0017] The present invention adds a screening and breeding stage, and puts fry of similar specifications into the same breeding pond, thereby preventing mutual aggression caused by size differences and loss caused by unbalanced feeding, which has a positive effect on improving the survival rate and compliance rate of tiger fish. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings: Figure 1 Schematic diagram of the breeding system of the present invention. DETAILED DESCRIPTION

[0019] The following examples illustrate the implementation methods of the present application in detail, so that the implementation process of how the present application applies technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0020] like Figure 1 As shown, the setting of the breeding system is as follows: several breeding ponds, several reaction ponds and a single balancing pond are designed as a water circulation system, and the several breeding ponds, several reaction ponds and the balancing pond are connected in sequence by pipes, and the balancing pond is connected to the breeding pond.

[0021] ① When the aquaculture pool drains water into the primary reaction pool, the primary reaction pool drains water into the secondary reaction pool, and the secondary reaction pool drains water into the balancing pool. When the water levels in the primary reaction pool, the secondary reaction pool, and the balancing pool all reach the set height, the water in the balancing pool is treated by the electrochemical water purification equipment and then pumped back into several aquaculture pools to form a circulation loop. The aquaculture pools are in a parallel relationship. ② After the water level in the breeding pond drops due to evaporation, the reservoir supplies water to the breeding pond and the water level in the breeding pond is maintained constant through the water level control switch; ③ MBBR filler (Type: mbbr19) is installed in the primary reaction tank; ④ Use an indoor closed-loop aquaculture system to effectively ensure water quality, control the ammonia nitrogen concentration below 0.5mg / L, and the nitrite concentration below 0.2mg / L, meeting the water quality requirements of Indonesian tiger fish for aquaculture water; at the same time, rely on the hydroxyl free radicals generated by electrochemical equipment to disinfect harmful bacteria and parasites, thereby maintaining the health of the fish.

[0022] The electrochemical water purification equipment involved uses the water purification equipment of the previously applied "A circulating water treatment system based on ecological farming".

[0023] Example 1 An ecological circulating aquaculture method for tiger fish comprises the following steps: (1) Cleaning of the aquaculture system: Clean the water storage tank, aquaculture tank, primary reaction tank, secondary reaction tank and balance tank and dry them for at least 1 day.

[0024] (2) System water cultivation stage: Add tap water to the system and keep it in operation; add urea (0.01 g / L) and brown sugar (0.1 g / L) to the secondary reaction tank, supplemented with aeration and water cultivation, and add composite bacteria at the same time to cultivate system microorganisms; add mixed algae to the culture pond every two days for one month, and then change to once a week; The composite bacteria are a mixture of ammonia-forming bacteria, nitrifying bacteria and denitrifying bacteria in a mass ratio of 2:2:1, and the amount of the composite bacteria added to the secondary reaction tank is 0.5 g / L; The mixed algae are a mixture of Chlorella vulgaris, Crescentella solani and Polygonum multiflorum (in a mass ratio of 2:1:1), and the algae density is 1×10 6 cfu / mL, and the single release amount in the culture pond is 50L.

[0025] (3) Training to eat artificial feed: 5,000 fry of 1.5-2 cm in size were transferred to the breeding pond and fed with brine shrimp for the first two weeks (100 g per meal, 400 g per day). After the fry were stable, a micro-spraying device was used in a corner of the breeding pond to create water disturbance. The water flow should not be too large. When the fish gathered near the micro-spraying device, artificial feed was placed in small amounts and multiple times on the disturbed water flow. As the water flow was disturbed, the fry would gradually start to eat. After each feeding, the uneaten artificial feed in the breeding pond was cleaned out in time. The fry were fed 4 times a day (50 g per meal, 200 g per day). The training lasted for 15 days to complete the transition from biological bait to artificial feed. The preparation method of the above-mentioned artificial feed is as follows: weigh the following raw materials in percentage: 20% of whiteleg shrimp meat, 40% of fish meal, 8% of soy protein concentrate, 14.8% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals (calculated by mass percentage, calcium lactate 30%, ferrous sulfate 6%, copper methionine 0.2%, chromium methionine 0.25%, zinc methionine 3%, sodium selenite 0.05%, magnesium sulfate 0.2%, calcium iodate 0.8%, zeolite powder 59.5%), and complex vitamins (calculated by mass percentage, vitamin A acetate 9%, vitamin D3 2.5%, vitamin E 20%, vitamin B1 6%, vitamin B2 5%, vitamin B6 4%, vitamin K3 1.5%, biotin 0.6%, folic acid 0.5%, inositol 11%, corn gluten meal 39.9%) 0.4%, compound probiotic fermentation sterilized liquid (mass ratio, lactic acid bacteria: yeast = 1:1) 10%; the above raw materials are mixed evenly, extruded into granules and dried to prepare artificial feed.

[0026] (4) Screening and breeding stage: The fry are screened and bred once every month. The feeding of artificial feed is stopped the day before each screening. By putting fry of similar sizes into the same breeding pond, the relative balance of fry sizes in each breeding pond is ensured, which can prevent mutual aggression caused by size differences and loss caused by unbalanced feeding. The fry are screened 4 times in total and then transferred to the next level of breeding; During the screening and breeding stage, artificial feed is fed 4 times a day, and the total daily feeding amount accounts for 2% of the total weight of the fry. The water temperature of the breeding pond is 28℃, and the dissolved oxygen is above 5mg / L.

[0027] (5) Growing stage: The healthy fry that are screened and transferred to the breeding pond are cultured continuously for 6 months, and lactic acid bacteria are fed once a week during this period.

[0028] During the growing stage, artificial feed is fed three times a day, and the total daily feeding amount accounts for 2% of the total weight of the fry. The water temperature of the breeding pond is 28°C, and the dissolved oxygen is above 5mg / L; based on the artificial feed feeding amount, the lactic acid bacteria feeding amount is 2g / kg.

[0029] Example 2 An ecological circulating aquaculture method for tiger fish comprises the following steps: (1) Cleaning of the aquaculture system: Clean the water storage tank, aquaculture tank, primary reaction tank, secondary reaction tank and balance tank and dry them for at least 1 day.

[0030] (2) System water cultivation stage: Add tap water to the system and keep it in operation; add urea (0.01 g / L) and brown sugar (0.1 g / L) to the secondary reaction tank, supplemented with aeration and water cultivation, and add composite bacteria at the same time to cultivate system microorganisms; add mixed algae to the culture pond every two days for one month, and then change to once a week; The composite bacteria are a mixture of ammonia-forming bacteria, nitrifying bacteria and denitrifying bacteria in a mass ratio of 2:2:1, and the amount of the composite bacteria added to the secondary reaction tank is 0.2 g / L; The mixed algae are a mixture of Chlorella, Crescentella and Multi-awn algae (by mass ratio of 2:1:1), with an algae density of 1×10 6 cfu / mL, and the single release amount in the culture pond is 50L.

[0031] (3) Training to eat artificial feed: 5,000 fry of 1.5-2 cm in size were transferred to the breeding pond and fed with brine shrimp for the first two weeks (100 g per meal, 400 g per day). After the fry were stable, a micro-spraying device was used in one corner of the breeding pond to create water disturbance. The water flow should not be too large. When the fish gathered near the micro-spraying device, artificial feed was placed in small amounts and multiple times on the disturbed water flow. As the water flow was disturbed, the fry would gradually start to eat. After each feeding, the uneaten artificial feed in the breeding pond was cleaned out in time. The fry were fed 4 times a day (50 g per meal, 200 g per day). The training lasted for 10 days to complete the transition from biological bait to artificial feed. The preparation method of the above-mentioned artificial feed is as follows: weigh the following raw materials in percentage: 20% of whiteleg shrimp meat, 40% of fish meal, 8% of soy protein concentrate, 14.8% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals (calculated by mass percentage, calcium lactate 30%, ferrous sulfate 6%, copper methionine 0.2%, chromium methionine 0.25%, zinc methionine 3%, sodium selenite 0.05%, magnesium sulfate 0.2%, calcium iodate 0.8%, zeolite powder 59.5%), and complex vitamins (calculated by mass percentage, vitamin A acetate 9%, vitamin D3 2.5%, vitamin E 20%, vitamin B1 6%, vitamin B2 5%, vitamin B6 4%, vitamin K3 1.5%, biotin 0.6%, folic acid 0.5%, inositol 11%, corn gluten meal 39.9%) 0.4%, compound probiotic fermentation sterilized liquid (mass ratio, lactic acid bacteria: yeast = 1:1) 10%; the above raw materials are mixed evenly, extruded into granules and dried to prepare artificial feed.

[0032] (4) Screening and breeding stage: The fry are screened and bred once every month. The feeding of artificial feed is stopped the day before each screening. By putting fry of similar sizes into the same breeding pond, the relative balance of fry sizes in each breeding pond is ensured, which can prevent mutual aggression caused by size differences and loss caused by unbalanced feeding. The fry are screened 4 times in total and then transferred to the next level of breeding; During the screening and breeding stage, artificial feed is fed 4 times a day, and the total daily feeding amount accounts for 2% of the total weight of the fry. The water temperature of the breeding pond is 28℃, and the dissolved oxygen is above 5mg / L.

[0033] (5) Growing stage: The healthy fry that are screened and transferred to the breeding pond are cultured continuously for 6 months, and lactic acid bacteria are fed once a week during this period.

[0034] During the growing stage, artificial feed is fed three times a day, and the total daily feeding amount accounts for 2% of the total weight of the fry. The water temperature of the breeding pond is 28°C, and the dissolved oxygen is above 5mg / L; based on the artificial feed feeding amount, the lactic acid bacteria feeding amount is 2g / kg.

[0035] Comparative Example 1 An ecological recirculating aquaculture method for tiger fish is basically the same as that of Example 1, except that the mixed algae are replaced with an equal amount of Chlorella, and the rest remain unchanged.

[0036] Comparative Example 2 An ecological circulating aquaculture method for tiger fish comprises the following steps: Steps (1)-(2) are the same as in Example 1.

[0037] (3) Training to feed artificial feed: 5,000 fry of 1.5-2 cm in size were transferred to the breeding pond and fed with brine shrimp for the first two weeks. After the fry were stable, artificial feed was added to the breeding pond in small amounts and multiple times. After each feeding, the uneaten artificial feed in the breeding pond was promptly cleaned out. Feeding was carried out 4 times a day for a total of 15 days to complete the transition from biological bait to artificial feed. The preparation method of the above-mentioned artificial feed is as follows: weigh the following raw materials in percentage: 20% of whiteleg shrimp meat, 40% of fish meal, 8% of soy protein concentrate, 14.8% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals (calculated by mass percentage, calcium lactate 30%, ferrous sulfate 6%, copper methionine 0.2%, chromium methionine 0.25%, zinc methionine 3%, sodium selenite 0.05%, magnesium sulfate 0.2%, calcium iodate 0.8%, zeolite powder 59.5%), and complex vitamins (calculated by mass percentage, vitamin A acetate 9%, vitamin D3 2.5%, vitamin E 20%, vitamin B1 6%, vitamin B2 5%, vitamin B6 4%, vitamin K3 1.5%, biotin 0.6%, folic acid 0.5%, inositol 11%, corn gluten meal 39.9%) 0.4%, compound probiotic fermentation sterilized liquid (mass ratio, lactic acid bacteria: yeast = 1:1) 10%; the above raw materials are mixed evenly, extruded into granules and dried to prepare artificial feed.

[0038] Steps (4)-(5) are the same as in Example 1.

[0039] Comparative Example 3 An ecological circulating aquaculture method for tiger fish comprises the following steps: Steps (1)-(2) are the same as in Example 1.

[0040] (3) Adaptation to artificial feed: 5,000 fry of 1.5-2 cm in size are transferred to the breeding pond. A micro-spray device is used in one corner of the breeding pond to create water disturbance. The water flow should not be too large. When the fish gather near the micro-spray device, artificial feed is placed in small amounts and multiple times on the disturbed water flow. As the water flow is disturbed, the fry will gradually start to eat. After each feeding, the uneaten artificial feed in the breeding pond is promptly cleaned out. Feed 4 times a day for a total of 15 days of adaptation. The preparation method of the above-mentioned artificial feed is as follows: weigh the following raw materials in percentage: 20% of whiteleg shrimp meat, 40% of fish meal, 8% of soy protein concentrate, 14.8% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals (calculated by mass percentage, calcium lactate 30%, ferrous sulfate 6%, copper methionine 0.2%, chromium methionine 0.25%, zinc methionine 3%, sodium selenite 0.05%, magnesium sulfate 0.2%, calcium iodate 0.8%, zeolite powder 59.5%), and complex vitamins (calculated by mass percentage, vitamin A acetate 9%, vitamin D3 2.5%, vitamin E 20%, vitamin B1 6%, vitamin B2 5%, vitamin B6 4%, vitamin K3 1.5%, biotin 0.6%, folic acid 0.5%, inositol 11%, corn gluten meal 39.9%) 0.4%, compound probiotic fermentation sterilized liquid (mass ratio, lactic acid bacteria: yeast = 1:1) 10%; the above raw materials are mixed evenly, extruded into granules and dried to prepare artificial feed.

[0041] Steps (4)-(5) are the same as in Example 1.

[0042] Comparative Example 4 An ecological circulating aquaculture method for tiger fish comprises the following steps: Steps (1) to (3) are the same as in Example 1.

[0043] (4) Growing stage: Healthy fry after feeding training are transferred to the breeding pond and continuously cultured until maturity. During this period, lactic acid bacteria are mixed and fed once a week. During the growing stage, artificial feed is fed three times a day, and the total daily feed amount accounts for 2% of the total body weight of the fry. The water temperature of the breeding pond is 28℃, and the dissolved oxygen is above 5mg / L. Based on the artificial feed feeding amount, the lactic acid bacteria feeding amount is 2g / kg.

[0044] Detection The Indonesian tiger fish of each group were cultured for 10 months according to the culture methods of Examples 1-2 and Comparative Examples 1-4. The compliance rate (length greater than 12 cm was considered to be qualified, and the compliance rate was the percentage of qualified fish to the total number of surviving fish) and the survival rate were tested. The specific results are shown in Table 1.

[0045] Table 1 Compliance rate and survival rate As shown in Table 1: (1) Compared with Example 1, in Comparative Example 1, only Chlorella was used to culture system microorganisms, which slightly affected the compliance rate and survival rate of tiger fish.

[0046] (2) Compared with Example 1, Comparative Example 2 did not adopt the flowing water feeding method, and the compliance rate and survival rate of tiger fish decreased.

[0047] (3) Compared with Example 1, in Comparative Example 3, the initial fry were directly fed with artificial feed, which resulted in a significant decrease in the survival rate of tiger fish and a significant impact on its compliance rate.

[0048] (4) Compared with Example 1, in Comparative Example 4, the fry were not screened and cultured, resulting in a significant decrease in the compliance rate of tiger fish and a significant impact on their survival rate.

[0049] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An ecological circulating water culture method for tiger fish, characterized in that: The following steps are involved: (1) Cleaning of the aquaculture system: Clean and air-dry the water storage tank, aquaculture tank, primary reaction tank, secondary reaction tank and balancing tank; (2) System water cultivation stage: Add tap water to the system and keep it in operation; add urea and brown sugar to the secondary reaction tank, supplement with aeration and water cultivation, and add composite bacteria at the same time; add mixed algae to the culture pond every two days for one month, and then change to once a week; (3) Training the fry to eat artificial feed: Transfer the fry to the breeding pond and feed them with brine shrimp for the first two weeks. After the fry are stable, use a micro-spray device in one corner of the breeding pond to create water disturbance and place artificial feed on the disturbed water flow. As the water flow is disturbed, the fry will gradually start to eat. Feed them 4 times a day for a total of 10-15 days to complete the transition from biological bait to artificial feed. (4) Screening and breeding stage: every month of breeding, the fry are screened and bred once, and the fry of similar size are put into the same breeding pond to ensure the relative balance of the fry size in each breeding pond. The fry are screened 4 times in total and then transferred to the next level of breeding; (5) Growing stage: The healthy fry that are screened and transferred to the breeding pond are cultured continuously for 6 months, and lactic acid bacteria are fed once a week during this period.

2. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (2), the amount of urea added is 0.01 g / L, and the amount of brown sugar added is 0.1 g / L.

3. The ecological circulating aquaculture method of tiger fish according to claim 1, characterized in that: In step (2), the composite bacteria is a strain composed of ammonia-forming bacteria, nitrifying bacteria and denitrifying bacteria in a mass ratio of 2:2:1, and the amount of the composite bacteria added to the secondary reaction tank is 0.2-0.5 g / L.

4. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (2), the mixed algae is a mixture of Chlorella vulgaris, Crescentella solani and Polygonum multiflorum in a mass ratio of 2:1:1, and the algae density is 1×10 6 cfu / mL, and the single release amount in the culture pond is 50L.

5. The ecological circulating aquaculture method of tiger fish according to claim 1, characterized in that: In step (3), the method for preparing the artificial feed is as follows: weigh the following raw materials in percentage: 15-20% of whiteleg shrimp meat, 35-40% of fish meal, 8% of soybean protein concentrate, 10-15% of high-gluten flour, 2.5% of fish oil, 1.5% of soybean oil, 2% of soybean lecithin, 0.8% of complex minerals, 0.4% of complex vitamins, and 5-10% of complex probiotic fermentation sterilization liquid; stir the above raw materials evenly, extrude and granulate them, and dry them to prepare the artificial feed.

6. The ecological circulating aquaculture method for tiger fish according to claim 5, characterized in that: In terms of mass percentage, the composite mineral consists of 30% calcium lactate, 6% ferrous sulfate, 0.2% copper methionine, 0.25% chromium methionine, 3% zinc methionine, 0.05% sodium selenite, 0.2% magnesium sulfate, 0.8% calcium iodate, and 59.5% zeolite powder; Calculated by mass percentage, the multivitamin consists of 9% vitamin A acetate, 2.5% vitamin D3, 20% vitamin E, 6% vitamin B1, 5% vitamin B2, 4% vitamin B6, 1.5% vitamin K3, 0.6% biotin, 0.5% folic acid, 11% inositol, and 39.9% corn gluten meal; The compound probiotic fermentation sterilized liquid is composed of lactic acid bacteria and yeast in a mass ratio of 1:

1.

7. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (3), after each feeding, the uneaten artificial feed in the breeding pond is cleaned out in time.

8. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (4), during the screening and breeding stage, artificial feed is fed 4 times a day, and the total daily feeding amount accounts for 1-2% of the total weight of the fry. The water temperature of the breeding pond is 28°C, and the dissolved oxygen is above 5 mg / L.

9. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (4), the feeding of artificial feed is stopped the day before each screening.

10. The ecological circulating aquaculture method for tiger fish according to claim 1, characterized in that: In step (5), during the growth stage, artificial feed is fed three times a day, and the total daily feeding amount accounts for 1-2% of the total weight of the fry. The water temperature of the culture pond is 28°C, and the dissolved oxygen is above 5 mg / L; based on the artificial feed feeding amount, the lactic acid bacteria feeding amount is 2 g / kg.

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