Feces water decomposing agent and culture process thereof

Abstract

The invention discloses a faeces water quality decomposing agent and a culture process thereof. The faeces water quality decomposing agent is prepared from the following components in parts by weight: 15 to 30 parts of photosynthetic bacteria, 10 to 20 parts of pseudogreen photosynthetic bacteria, 3.75 to 7.5 parts of bacillus amyloliquefaciens, 5 to 10 parts of proteolytic enzyme, 2 to 4 parts of humic acid, 0.5 to 1 part of sodium alginate and 1 to 2 parts of monopotassium phosphate. By optimizing the strain combination and auxiliary component proportion, an efficient and synergistic microbial ecological system is formed, various organic matters in the excrement can be quickly decomposed, and the organic fertilizer is suitable for wide organic waste treatment of human, livestock and poultry excrement and the like; a pure biological technology is adopted, the method is environment-friendly and pollution-free, and decomposed products can be used as high-quality organic fertilizers to realize resource circulation; the culture process is scientific and reasonable, parameters of each step are clear, links from strain activation to enzyme preparation and the like are all optimized, and the quality and stability of the decomposer are improved; the raw materials are wide in source and low in price, the culture process is simple, and the treatment cost is greatly reduced.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of water quality decomposing agent, in particular to a fecal water quality decomposing agent and a culture process thereof. BACKGROUND

[0002] In the field of environmental protection and resource recycling, the harmless and resourceful treatment of feces has always been an important issue of concern. With the growth of population and the acceleration of urbanization process, the amount of feces is increasing day by day. If not properly treated, it will not only cause serious environmental pollution, lead to water eutrophication, soil pollution and other environmental problems, but also spread diseases and endanger human health. Therefore, it is of great practical significance to develop efficient and environmentally friendly feces treatment technology.

[0003] At present, there are many methods for treating feces, among which the use of microorganisms to decompose feces is a common and potential technical means. For example, the prior art CN109439610A discloses a human feces decomposition culture medium, the raw materials of which include, by weight: 20-30 parts of corn flour, 15-25 parts of mulberry leaf powder, 6-12 parts of potato powder, 2-6 parts of biochar, 5-10 parts of wood ash, 3-7 parts of sugarcane residue, 2-4 parts of sucrose, 3-5 parts of borax, 1-3 parts of amino acid, 0.3-0.8 parts of glucose, 0.6-1.0 parts of magnesium sulfate, 1-2 parts of dipotassium hydrogen phosphate, 6-10 parts of agar, 10-16 parts of compound microbial agent, and 50-90 parts of water. The feces decomposition culture medium has a high growth rate and decomposition efficiency of the compound microbial agent through a scientific and reasonable formula and ratio, which can effectively improve the decomposition efficiency of organic matter and realize the harmless and resourceful treatment of feces.

[0004] However, the existing feces decomposition technology still has some deficiencies. On the one hand, some culture medium ingredients are complex, the preparation process is tedious, and the cost is high, which is not conducive to large-scale popularization and application; on the other hand, the microbial strain combination in some culture media is not optimized, and the decomposition of specific components in feces is not strong enough, resulting in unsatisfactory decomposition effect, which is difficult to meet the increasingly strict environmental protection requirements and treatment needs. Therefore, it is of great urgency and necessity to develop a fecal water quality decomposing agent with simple ingredients, easy preparation, low cost and significant decomposition effect, and a culture process thereof. SUMMARY

[0005] The purpose of this invention is to provide a fecal water decomposing agent and its cultivation process. By optimizing the combination of bacterial strains and the ratio of auxiliary components, a highly efficient and synergistic microbial ecosystem is formed, which can rapidly decompose various organic substances in feces. It is applicable to the treatment of a wide range of organic wastes, including human and livestock manure. It adopts pure biological technology, which is environmentally friendly and pollution-free, and the decomposition products can be used as high-quality organic fertilizer to achieve resource recycling. The cultivation process is scientific and reasonable, with clear parameters for each step. From bacterial activation to enzyme preparation, all links have been optimized to improve the quality and stability of the decomposing agent. The raw materials are widely available and inexpensive, and the cultivation process is simple, significantly reducing treatment costs. It is convenient to use, requiring no complex operations or professional personnel, and has high operability and practicality, making it highly valuable for large-scale promotion and application.

[0006] To achieve the above objectives, the main technical solutions adopted by the present invention include: A fecal water decomposing agent, comprising the following components by weight: 15-30 parts of photosynthetic bacteria, 10-20 parts of pseudogreen photosynthetic bacteria, 3.75-7.5 parts of Bacillus amyloliquefaciens, 5-10 parts of proteolytic enzymes, 2-4 parts of humic acid, 0.5-1 part of sodium alginate, and 1-2 parts of potassium dihydrogen phosphate.

[0007] The above-mentioned fecal water decomposing agent comprises, by weight, the following components: 15 parts of photosynthetic bacteria, 10 parts of pseudogreen photosynthetic bacteria, 3.75 parts of Bacillus amyloliquefaciens, 5 parts of proteolytic enzymes, 2 parts of humic acid, 0.5 parts of sodium alginate, and 1 part of potassium dihydrogen phosphate.

[0008] The above-mentioned fecal water decomposing agent comprises, by weight, the following components: 22 parts of photosynthetic bacteria, 15 parts of pseudogreen photosynthetic bacteria, 5 parts of Bacillus amyloliquefaciens, 7 parts of proteolytic enzymes, 3 parts of humic acid, 0.8 parts of sodium alginate, and 1.25 parts of potassium dihydrogen phosphate.

[0009] The above-mentioned fecal water decomposing agent comprises, by weight, the following components: 30 parts of photosynthetic bacteria, 20 parts of pseudogreen photosynthetic bacteria, 7.5 parts of Bacillus amyloliquefaciens, 10 parts of proteolytic enzymes, 4 parts of humic acid, 1 part of sodium alginate, and 2 parts of potassium dihydrogen phosphate.

[0010] A process for cultivating a fecal water decomposing agent includes the following steps: S1. Activation of bacterial strains: The bacterial strains of photosynthetic bacteria, pseudogreen photosynthetic bacteria, and Bacillus amyloliquefaciens are inoculated into the corresponding culture media for activation culture. The culture medium for photosynthetic bacteria is photosynthetic bacteria culture medium, whose main components include ammonium chloride, potassium dihydrogen phosphate, magnesium sulfate, and yeast extract. The culture media for pseudogreen photosynthetic bacteria and Bacillus amyloliquefaciens can be nutrient broth culture medium.

[0011] S2. Expanded culture: The activated strain is inoculated into the expanded culture medium at a certain ratio for expanded culture. The culture conditions are similar to those of the strain activation stage, but the culture time and inoculation amount will be optimized according to the actual situation.

[0012] S3. Enzyme preparation: Microorganisms that produce protein hydrolases are inoculated into a fermentation medium for fermentation culture. The main components of the fermentation medium include peptone, yeast extract, glucose, sodium chloride, etc. After fermentation, the fermentation broth is collected by centrifugation, filtration and other methods. The protein hydrolases are then purified by ultrafiltration, salting out and other purification techniques to obtain a high-purity protein hydrolases solution.

[0013] S4. Mixing and blending: Mix the expanded culture of photosynthetic bacteria, pseudogreen photosynthetic bacteria, and Bacillus amyloliquefaciens in a certain proportion, then add the prepared protein hydrolase solution, and then add auxiliary ingredients such as humic acid, sodium alginate, and potassium dihydrogen phosphate in sequence, and stir thoroughly to ensure that all components are fully mixed.

[0014] S5. Drying and Packaging: The mixed materials are dried using methods such as spray drying or freeze drying to remove moisture, improve the stability and shelf life of the decomposing agent, and then the dried product is crushed and sieved to obtain a uniform powdered fecal water decomposing agent. Finally, it is packaged to obtain the finished product.

[0015] The above-mentioned fecal water decomposition agent cultivation process, wherein the cultivation conditions of the photosynthetic bacteria in S1 are light intensity of 2000-3000 lx, temperature of 25-30℃, and cultivation time of 3-5 days.

[0016] The culture process of the above-mentioned fecal water decomposing agent, wherein the culture conditions of Pseudomonas aeruginosa and Bacillus amyloliquefaciens in S1 are a temperature of 30-35℃, a rotation speed of 150-200r / min, and a culture time of 24-48h.

[0017] In the above-mentioned culture process of fecal water decomposition agent, the composition of the expansion culture medium in S2 can be appropriately adjusted according to the needs of different bacterial strains, generally including carbon source, nitrogen source and inorganic salt, in order to obtain a sufficient number of highly active bacterial cells.

[0018] In the above-mentioned process for cultivating fecal water decomposing agent, the microorganism in S3 is Bacillus subtilis.

[0019] The above-mentioned process for cultivating fecal water decomposing agent includes fermentation conditions in S3 of 30-35℃, 200-250r / min, and 48-72h.

[0020] Compared with the prior art, the advantages and positive effects of the present invention are as follows: 1. This invention, through optimizing the combination of microbial strains and the ratio of auxiliary components, forms a highly efficient and synergistic microbial ecosystem that can rapidly decompose various organic substances in feces, applicable to the treatment of a wide range of organic wastes such as human and livestock manure; it adopts pure biological technology, is environmentally friendly and pollution-free, and the decomposition products can be used as high-quality organic fertilizer to achieve resource recycling; the cultivation process is scientific and reasonable, with clear parameters for each step, and all links from strain activation to enzyme preparation have been optimized to improve the quality and stability of the decomposing agent; the raw materials are widely available and inexpensive, the cultivation process is simple, and the treatment cost is significantly reduced; it is convenient to use, requiring no complex operations or professional personnel, and has high operability and practicality, making it extremely valuable for large-scale promotion and application; 2. Highly Efficient Decomposition Performance: The fecal water decomposition agent of this invention utilizes carefully screened and optimized combinations of various bacterial strains, including photosynthetic bacteria, pseudogreen photosynthetic bacteria, and Bacillus amyloliquefaciens, along with a rationally proportioned blend of auxiliary components such as protein hydrolysates, humic acid, sodium alginate, and potassium dihydrogen phosphate, to form a powerful synergistic microbial ecosystem. These bacterial strains and components work together to rapidly and efficiently decompose organic matter in feces, including proteins, carbohydrates, and fats, converting them into harmless small molecules such as carbon dioxide, water, and inorganic salts. This significantly improves fecal decomposition efficiency and effectively shortens the treatment cycle.

[0021] 3. Wide Applicability: The fecal water decomposing agent of this invention is not only suitable for the treatment of human excrement, but can also be widely used in the treatment of various organic wastes such as livestock and poultry manure. Whether for centralized large-scale livestock farm manure treatment or decentralized rural household manure treatment, it can achieve good decomposition effects and has broad application prospects.

[0022] 4. Environmentally friendly and pollution-free: Compared with traditional chemical treatment methods, the fecal water decomposition agent of this invention uses pure biological technology, which does not produce secondary pollution during the decomposition process and will not harm the soil, water bodies, or air. At the same time, the decomposition products can be used as high-quality organic fertilizer, realizing the recycling of resources and conforming to the concept of sustainable development.

[0023] 5. Optimized Cultivation Process: The cultivation process of the fecal water decomposing agent of this invention is meticulously designed. From strain activation and scale-up cultivation to enzyme preparation, mixing and formulation, and drying and packaging, each step has clearly defined technical parameters and operational points. For example, in the strain activation stage, appropriate light intensity, temperature, and cultivation time are adopted according to the characteristics of different strains to ensure that the strains are fully activated and maintain good activity. In the scale-up cultivation stage, the composition of the scale-up culture medium is adjusted according to the needs of different strains to obtain a sufficient number of highly active cells. In the enzyme preparation stage, Bacillus subtilis is selected as the microorganism for producing protein hydrolysates, and the yield and purity of protein hydrolysates are improved by optimizing fermentation conditions. These optimization measures make the cultivation process more scientific and reasonable, improving the quality and stability of the decomposing agent.

[0024] 6. Low cost: The raw materials for the fecal water decomposition agent of this invention are widely available and relatively inexpensive. Furthermore, the cultivation process is simple and easy to implement, requiring no complex equipment or high-cost investment. Compared with some existing fecal decomposition technologies, it significantly reduces treatment costs, resulting in higher economic benefits and facilitating large-scale application.

[0025] 7. Simple Operation: The fecal water decomposing agent of this invention is easy to use. Simply add an appropriate amount of the decomposing agent to the feces and stir well. No complicated operating procedures or professional technicians are required. This makes the decomposing agent highly operable and practical in real-world applications, meeting the needs of different users. Attached Figure Description

[0026] The accompanying drawings, which are included to provide a further understanding of this application and form part of this application, illustrate exemplary embodiments and are used to explain this application, but do not constitute an undue limitation of this application. In the drawings: Figure 1 This is a flowchart of the cultivation process for the fecal water decomposing agent of the present invention. Detailed Implementation

[0027] The following will describe in detail the implementation of this application with reference to the accompanying drawings and embodiments, so that the implementation process of how this application uses technical means to solve technical problems and achieve technical effects can be fully understood and implemented accordingly.

[0028] Example 1 Please refer to Figure 1 As shown, an embodiment of the present invention provides a fecal water decomposing agent, which comprises the following components by weight: 15-30 parts of photosynthetic bacteria, 10-20 parts of pseudogreen photosynthetic bacteria, 3.75-7.5 parts of Bacillus amyloliquefaciens, 5-10 parts of proteolytic enzymes, 2-4 parts of humic acid, 0.5-1 part of sodium alginate, and 1-2 parts of potassium dihydrogen phosphate.

[0029] The above technical solution has the following beneficial effects: Synergistic decomposition by multiple microorganisms: photosynthetic bacteria degrade sulfides, pseudogreen photosynthetic bacteria decompose proteins, and Bacillus amyloliquefaciens decomposes carbohydrates, covering the main organic components in feces, thus greatly improving decomposition efficiency.

[0030] Enzyme-bacterial enhancement system: Exogenous protein hydrolytic enzymes directly catalyze protein hydrolysis, shortening the decomposition cycle.

[0031] Enhanced environmental adaptability: Humic acid chelates heavy metals, sodium alginate protects bacterial cell activity, and potassium dihydrogen phosphate adjusts pH, enabling the decomposing agent to function stably within the pH range of 6-9 and the temperature range of 15-40℃.

[0032] Example 2 Please refer to Figure 1 As shown, an embodiment of the present invention provides a fecal water decomposing agent, which comprises the following components by weight: 15 parts of photosynthetic bacteria, 10 parts of pseudogreen photosynthetic bacteria, 3.75 parts of Bacillus amyloliquefaciens, 5 parts of proteolytic enzymes, 2 parts of humic acid, 0.5 parts of sodium alginate, and 1 part of potassium dihydrogen phosphate.

[0033] The above technical solution has the following beneficial effects: Cost optimization: Reduce raw material costs by decreasing the amount of microbial agents and enzymes used, while maintaining basic decomposition efficiency.

[0034] Lightweight applications: Suitable for low-concentration organic wastewater (such as household septic tanks), reducing resource waste caused by excessive dosage.

[0035] Example 3 Please refer to Figure 1 As shown, an embodiment of the present invention provides a fecal water decomposing agent, which comprises the following components by weight: 22 parts of photosynthetic bacteria, 15 parts of pseudogreen photosynthetic bacteria, 5 parts of Bacillus amyloliquefaciens, 7 parts of proteolytic enzymes, 3 parts of humic acid, 0.8 parts of sodium alginate, and 1.25 parts of potassium dihydrogen phosphate.

[0036] The above technical solution has the following beneficial effects: Balancing efficiency and cost: After optimizing the bacteria-enzyme ratio, the decomposition speed was significantly improved compared to Example 2, while the raw material cost increased only slightly.

[0037] Wide applicability: Suitable for wastewater treatment in medium-sized farms, balancing economy and treatment effectiveness.

[0038] Example 4 Please refer to Figure 1 As shown, an embodiment of the present invention provides a fecal water decomposing agent, which comprises the following components by weight: 30 parts of photosynthetic bacteria, 20 parts of pseudogreen photosynthetic bacteria, 7.5 parts of Bacillus amyloliquefaciens, 10 parts of proteolytic enzymes, 4 parts of humic acid, 1 part of sodium alginate, and 2 parts of potassium dihydrogen phosphate.

[0039] The above technical solution has the following beneficial effects: High-efficiency treatment of high-concentration wastewater: For high-load organic wastewater (such as biogas slurry from large-scale pig farms), the decomposition efficiency is improved compared to Example 3.

[0040] Long-lasting stability: High concentrations of microbial agents and stabilizers (humic acid, sodium alginate) extend the shelf life of the decomposition agent to more than 6 months, reducing the need for frequent addition.

[0041] Example 5 Please refer to Figure 1 As shown, an embodiment of the present invention provides a cultivation process for a fecal water decomposing agent, comprising the following steps: S1, strain activation 1. Photosynthetic bacteria-like bacteria: Inoculate the photosynthetic bacteria-like bacteria into the photosynthetic bacteria culture medium, and culture for 4 days under a light intensity of 2500 lx and a temperature of 28℃.

[0042] 2. Pseudogreen photosynthetic bacteria: Pseudogreen photosynthetic bacteria were inoculated into nutrient broth medium and cultured at 32℃ and 180r / min for 36h.

[0043] 3. Bacillus amyloliquefaciens: Inoculate Bacillus amyloliquefaciens into nutrient broth medium, and incubate at 33℃ and 170 r / min for 30 h.

[0044] S2, Expanded cultivation 1. Photosynthetic bacteria: Inoculate the activated photosynthetic bacteria strain into the expansion medium at an inoculum rate of 5%, and incubate for 5 days at a light intensity of 2500 lx and a temperature of 28℃.

[0045] 2. Pseudogreen photosynthetic bacteria: The activated pseudogreen photosynthetic bacteria strain was inoculated into the expansion medium at an inoculation rate of 3%, and cultured at 32℃ and 180r / min for 48h.

[0046] 3. Bacillus amyloliquefaciens: Inoculate the activated Bacillus amyloliquefaciens strain into the expansion medium at an inoculation rate of 4%, and incubate at 33℃ and 170 r / min for 40 h.

[0047] S3, Enzyme Preparation Bacillus subtilis was inoculated into the fermentation medium and fermented at 32℃ and 220 r / min for 60 h. After fermentation, the fermentation broth was collected by centrifugation and filtration, and the proteolytic enzymes were purified by ultrafiltration and salting out to obtain a proteolytic enzyme solution.

[0048] S4, Mixing and blending Mix the expanded culture of photosynthetic bacteria, pseudogreen photosynthetic bacteria, and Bacillus amyloliquefaciens in a 2:1:1 ratio, then add the prepared protein hydrolase solution, followed by 5% humic acid, 3% sodium alginate, and 2% potassium dihydrogen phosphate, and stir thoroughly.

[0049] S5, Dry Packaging The mixed and formulated materials are spray-dried, and the dried product is crushed and sieved to obtain a uniform powdered fecal water decomposition agent, which is then packaged to obtain the finished product.

[0050] The above technical solution has the following beneficial effects: Process standardization: By precisely controlling parameters such as light, temperature, and rotation speed, the consistency of microbial agent activity is ensured.

[0051] Preparation of highly active bacterial cells: Optimizing the inoculum size and time during the expanded culture stage greatly increases the bacterial cell density and significantly enhances the decomposition ability.

[0052] Spray drying improves efficiency: The product has low moisture content and dissolves quickly, making it suitable for automated dosing equipment.

[0053] Example 6 Please refer to Figure 1 As shown, an embodiment of the present invention provides a culture process for a fecal water decomposition agent, comprising the following steps: S1, bacterial activation. 1. Photosynthetic bacteria-like bacteria: Inoculate the photosynthetic bacteria-like bacteria into the photosynthetic bacteria culture medium, and culture for 5 days under a light intensity of 2000 lx and a temperature of 25℃.

[0054] 2. Pseudogreen photosynthetic bacteria: Inoculate the Pseudogreen photosynthetic bacteria into a nutrient broth medium, and incubate at 30℃ and 150r / min for 48h.

[0055] 3. Bacillus amyloliquefaciens: Inoculate Bacillus amyloliquefaciens into nutrient broth medium, and culture at 30℃ and 150 r / min for 48 h.

[0056] S2, Expanded cultivation 1. Photosynthetic bacteria: Inoculate the activated photosynthetic bacteria strain into the expansion medium at an inoculum rate of 3%, and culture for 6 days under a light intensity of 2000 lx and a temperature of 25℃.

[0057] 2. Pseudogreen photosynthetic bacteria: Inoculate the activated pseudogreen photosynthetic bacteria into the expansion medium at an inoculation rate of 2%, and incubate at 30℃ and 150r / min for 60h.

[0058] 3. Bacillus amyloliquefaciens: Inoculate the activated Bacillus amyloliquefaciens strain into the expansion medium at an inoculation rate of 3%, and incubate at 30℃ and 150 r / min for 50 h.

[0059] S3, Enzyme Preparation Bacillus subtilis was inoculated into the fermentation medium and fermented at 30℃ and 200 rpm for 72 h. After fermentation, the fermentation broth was collected by centrifugation and filtration, and the proteolytic enzymes were purified by ultrafiltration and salting out to obtain a proteolytic enzyme solution.

[0060] S4, Mixing and blending The expanded culture of photosynthetic bacteria, pseudogreen photosynthetic bacteria, and Bacillus amyloliquefaciens was mixed evenly in a ratio of 3:1:1. Then, the prepared protein hydrolase solution was added, followed by 3% humic acid, 2% sodium alginate, and 1% potassium dihydrogen phosphate. The mixture was stirred thoroughly.

[0061] S5, Dry Packaging The mixed and formulated materials are freeze-dried, and the dried product is crushed and sieved to obtain a uniform powdered fecal water decomposition agent, which is then packaged to obtain the finished product.

[0062] The above technical solution has the following beneficial effects: Low-temperature processing protects activity: Freeze-drying avoids damage to bacteria and enzymes caused by high temperatures, greatly improving product survival rate.

[0063] Low-residue additives: reduce the amount of humic acid and other substances used, reduce the impact on water color, and are suitable for landscape water treatment.

[0064] Example 7 Please refer to Figure 1 As shown, the effect verification of the culture process of the fecal water decomposing agent provided by the embodiments of the present invention is as follows: S1. Two poultry and livestock farms of the same size were selected and designated as the experimental farm and the control farm, respectively. In the experimental farm, the manure decomposing agent prepared in Example 1 of this invention was used, with a dosage of 10 kg per ton of manure for rapid decomposition and 5 kg less for slow decomposition; in pig pens, 25 g per square meter was applied via direct spraying. No manure decomposing agent was used in the control farm.

[0065] S2. After 30 days of continuous use, the decomposition of feces, odor levels, and water quality indicators of the two farms were tested. The results showed that the feces decomposition rate in the experimental farm was significantly faster than in the control farm. The levels of harmful substances such as ammonia nitrogen, nitrite, and hydrogen sulfide in the feces were significantly reduced, and the odor was significantly lessened. The dissolved oxygen content in the water increased, the pH was suitable, and the number of harmful microorganisms decreased. In contrast, the feces decomposition in the control farm was slow, the odor was severe, and the water quality indicators were poor. This indicates that the feces decomposition agent of this invention has excellent feces decomposition and odor removal effects, and can effectively improve the water quality environment.

[0066] By adopting the above technical solution: Field application verification: The concentrations of ammonia nitrogen and hydrogen sulfide in the test site were greatly reduced, the odor level met the national standard, and the dissolved oxygen was greatly increased.

[0067] Ecological safety: The number of harmful microorganisms (such as E. coli) in the water body is reduced by 90%, proving that the decomposition agent does not cause secondary pollution.

[0068] The working principle of the present invention can be summarized from the above embodiments as follows: Bacteria-enzyme synergistic decomposition mechanism: photosynthetic bacteria fix CO2 and degrade sulfides through photosynthesis, pseudogreen photosynthetic bacteria secrete proteases to hydrolyze proteins, Bacillus amyloliquefaciens decomposes carbohydrates, and exogenous protein hydrolytic enzymes produced by Bacillus subtilis enhance the degradation of macromolecular organic matter, forming a complete chain of "decomposition-conversion-stabilization".

[0069] Multifunctional additive regulation: humic acid chelates heavy metals, sodium alginate forms a protective film, and potassium dihydrogen phosphate adjusts pH, together constructing a stable microenvironment and enhancing the stress resistance of the bacterial agent (such as resistance to high temperature and high salt).

[0070] Process-Product-Application Closed Loop: Standardized cultivation process ensures high activity of bacterial agents, and spray / freeze-drying process adapts to different scenario requirements, ultimately achieving efficient and harmless decomposition of organic matter in feces and water purification.

[0071] The foregoing description illustrates and describes several preferred embodiments of the present invention. However, as previously stated, it should be understood that the present invention is not limited to the forms disclosed herein and should not be construed as excluding other embodiments. It can be used in various other combinations, modifications, and environments, and can be altered within the scope of the inventive concept described herein through the foregoing teachings or techniques or knowledge in related fields. Any modifications and variations made by those skilled in the art that do not depart from the spirit and scope of the present invention should be within the protection scope of the appended claims.

Claims

1. A fecal water quality decomposing agent, characterized by, The following components are included by weight parts: 15~30 parts of photosynthetic-like bacteria, 10~20 parts of pseudomonas chlororaphis, 3.75~7.5 parts of bacillus amyloliquefaciens, 5~10 parts of protein hydrolytic enzyme, 2~4 parts of humic acid, 0.5~1 part of sodium alginate, and 1~2 parts of potassium dihydrogen phosphate.

2. The feces water decomposing agent according to claim 1, characterized by: The following components are included by weight parts: 15 parts of photosynthetic-like bacteria, 10 parts of pseudomonas chlororaphis, 3.75 parts of bacillus amyloliquefaciens, 5 parts of protein hydrolytic enzyme, 2 parts of humic acid, 0.5 parts of sodium alginate, and 1 part of potassium dihydrogen phosphate.

3. The feces water decomposing agent according to claim 2, characterized by: The following components are included by weight parts: 22 parts of photosynthetic-like bacteria, 15 parts of pseudomonas chlororaphis, 5 parts of bacillus amyloliquefaciens, 7 parts of protein hydrolytic enzyme, 3 parts of humic acid, 0.8 parts of sodium alginate, and 1.25 parts of potassium dihydrogen phosphate.

4. The feces water decomposing agent according to claim 3, characterized by: The following components are included by weight parts: 30 parts of photosynthetic-like bacteria, 20 parts of pseudomonas chlororaphis, 7.5 parts of bacillus amyloliquefaciens, 10 parts of protein hydrolytic enzyme, 4 parts of humic acid, 1 part of sodium alginate, and 2 parts of potassium dihydrogen phosphate.

5. A culture process of a fecal water quality decomposing agent, characterized by: The following steps are included: S1, strain activation: the strains of photosynthetic-like bacteria, pseudomonas chlororaphis, and bacillus amyloliquefaciens are inoculated into the corresponding culture medium for activation culture, the culture medium for photosynthetic-like bacteria adopts photosynthetic bacteria culture medium, the main components of which include ammonium chloride, potassium dihydrogen phosphate, magnesium sulfate, and yeast extract, and the culture medium for pseudomonas chlororaphis and bacillus amyloliquefaciens can adopt nutrient broth medium. S2, scale-up culture: the activated strains are inoculated into the scale-up culture medium in a certain proportion for scale-up culture, the culture conditions are similar to those in the strain activation stage, but the culture time and inoculation amount can be optimized according to the actual situation. S3, enzyme preparation: the microorganism producing protein hydrolytic enzyme is inoculated into the fermentation culture medium for fermentation culture, the main components of the fermentation culture medium include peptone, yeast extract, glucose, sodium chloride, etc., after fermentation, the fermentation broth is collected by centrifugation, filtration, etc., and the protein hydrolytic enzyme is purified by ultrafiltration, salting-out, etc., to obtain a high-purity protein hydrolytic enzyme solution. S4, mixing and blending: the photosynthetic-like bacteria, pseudomonas chlororaphis, and bacillus amyloliquefaciens liquid after scale-up culture are mixed uniformly in a certain proportion, then the prepared protein hydrolytic enzyme solution is added, and then humic acid, sodium alginate, potassium dihydrogen phosphate, and other auxiliary components are added in turn, and stirred uniformly to fully mix the components. S5, drying and packaging: the mixed and blended material is dried, which can be dried by spray drying, freeze drying, etc., to remove the water in the material, improve the stability and shelf life of the decomposition agent, and then the dried product is crushed and sieved to obtain a uniform powder-shaped fecal water quality decomposition agent, which is then packaged to obtain the finished product.

6. The culture process of the excrement water quality decomposing agent according to claim 5, characterized in that: The culture conditions for photosynthetic-like bacteria in S1 are light intensity of 2000-3000lx, temperature of 25-30℃, and culture time of 3-5 days.

7. The culture process of the excrement water quality decomposing agent according to claim 6, characterized in that: The culture conditions of the Pseudomonas viridis and Bacillus amyloliquefaciens in S1 are 30-35℃, 150-200r / min, and 24-48h.

8. The culture process of the excrement water quality decomposing agent according to claim 7, characterized in that: The components of the expanded medium in S2 can be adjusted according to the requirements of different bacteria, and generally include carbon source, nitrogen source, and inorganic salt, so as to obtain a sufficient number of high-activity bacteria.

9. The culture process of the excrement water quality decomposing agent according to claim 8, characterized in that: The microorganism in S3 is Bacillus subtilis.

10. The culture process of the excrement water quality decomposing agent according to claim 9, characterized in that: The fermentation conditions in S3 are 30-35℃, 200-250r / min, and 48-72h.

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