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Method for improving imbitional chilling injury resistance of plant seeds

A technology for imbibition and chilling damage and plant seeds, which is applied to the application of n-butanol and N-acylethanolamine in improving the resistance of seeds to imbibition and chilling damage, and the treatment field of improving the resistance of plant seeds to imbibition and chilling damage, which can solve the problem of shortening the emergence time, Problems such as poor quality of seedlings and low emergence rate can shorten the emergence time, improve the ability to resist imbibition and cold damage, and improve the emergence rate.

Inactive Publication Date: 2015-04-29
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide a seed treatment method for improving the resistance of seeds to imbibition and chilling damage, so as to solve the problems of slow emergence, low emergence rate, and poor quality of seedlings caused by seeds encountering imbibition and chilling damage that are ubiquitous in agricultural production. , after exogenous application of n-butanol and N-acyl ethanolamine treatment by the present invention, the ability of seeds to resist imbibition and chilling damage can be improved, the emergence rate can be improved, and the emergence time can be shortened

Method used

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  • Method for improving imbitional chilling injury resistance of plant seeds

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Embodiment 1

[0015] n-butanol treatment system: n-butanol was prepared with deionized water, and the final treatment concentrations were 0.01%, 0.05%, and 0.08%. Control-1, directly treated with deionized water.

[0016] N-acylethanolamine (NAE) treatment system: three treatment solutions: (1) control-1, directly treated with deionized water; (2) control-2: the same volume as 5 μmol, 10 μmol, 25 μmol of dimethyl sulfoxide (DMSO), that is, the solubility of DMSO is 0.05%, 0.1%, 0.25%; (3) NAE12:0 is first dissolved in DMSO to prepare a 10 mmol mother liquor, and then diluted to the final treatment concentration: 5 μmol, 10 μmol, 25 μmol.

[0017] Imbibition and chilling sensitive seeds: NAE and n-butanol were applied to cucumber and pea during imbibition and chilling and germination, respectively. Imbibition and chilling were treated at 4°C for 24 hours and then transferred to moist filter paper, and placed in a constant temperature light incubator at 25°C. Germination was carried out unde...

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Abstract

The invention relates to a treatment method for improving the imbitional chilling injury resistance of plant seeds. The treatment method comprises the following steps: preparing normal butanol with deionized water, diluting until the concentration of the normal butanol is 0.01-0.08%, performing the imbitional chilling injury treatment on plant seeds, and performing external source addition treatment during germination. The invention belongs to the field of a crops treatment technology and in particular relates to a seed treatment method. Sensitive seeds are externally added with butyl alcohol or N-acyl ethanol in an imbitional chilling injury process at 4 DEG C, the tolerance of the sensitive seeds in an imbitional chilling injury process is improved remarkably, the crop seeds still have the high germination rate, relatively short seedling maturing period and high mature seedling quality, the yield of the crops is increased, and the method has the good application prospect.

Description

technical field [0001] The invention belongs to the technical field of crop treatment, in particular to a treatment method for improving the resistance of plant seeds to imbibition and chilling damage, and more particularly to the application of n-butanol and N-acylethanolamine in improving the resistance of seeds to imbibition and chilling damage. Background technique [0002] Seeds occupy a special and important position in agricultural production. In order to make the potential of seeds with high yield and high quality genetic characteristics into actual productivity, the primary link in cultivation is to maintain high vigor of the seeds, and obtain healthy and strong seedlings. However, many cultivated crops suffer from imbibition and chilling damage in the initial stage of seed germination. Seed imbibition and chilling injury is the imbibition damage of seeds at low temperature (Zheng Guanghua et al., 2001), which is serious not only in alpine regions and low-temperatur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 李唯奇禹晓梅
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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