12 results about "Natural variation" patented technology

The invention discloses an excellent haplotype of a corn salt tolerance regulation gene ZmSPL12 and application of the excellent haplotype. Through next-generation sequencing, a specific primer pair, PCR amplification and agarose gel electrophoresis, it is unexpectedly found that insertion / deletion variation of a fragment of about 5.2 kb in a ZmSPL12 promoter region causes a plant to be divided into two haplotypes, namely Hap1 and Hap2, the expression quantity of the ZmSPL12 gene of the Hap2 (deletion type) is higher, the salt tolerance is higher, and the ZmSPL12 gene of the Hap2 (deletion type) is higher than that of the ZmSPL12 gene of the Hap2 (deletion type). Available natural variation resources are provided for molecular marker-assisted breeding, and the problems of high biosafety approval cost and long time in biotechnology breeding are solved.

The invention discloses a promoter cis-acting element for regulating and controlling APO1 gene expression and application of the promoter cis-acting element, and belongs to the technical field of plant genetic engineering. From the perspective of APO1 gene expression regulation, a promoter cis-regulation region CRE3 for determining APO1 gene expression is found, and it is proved that a strain with the number of grains per ear and the yield of a single plant remarkably improved can be obtained by conducting editing knockout on a CRE3 sequence through CRISPER / Cas9, two-year plot testing shows that the yield is obviously increased, the stem diameter is increased, and the yield is obviously increased. The method can realize improvement of lodging resistance, can be used for rapid improvement of panicle type and yield of rice varieties, is superior to natural variation alleles in performance, and has extremely high application value.

The invention discloses a virus disease resistance gene and application of excellent natural variation of the virus disease resistance gene, and belongs to the technical field of biological breeding and vegetable production. The anti-ToCV function of the SlAVE3 gene is identified for the first time, the antiviral mechanism of the SlAVE3 gene for targeted degradation of virus p59 protein through a UPS system and regulation and control of transcription positive feedback circulation is clarified, and the blank of tomato ToCV resistance gene identification is filled. The invention provides a mutation form of the SlAVE3, clarifies the influence of key site mutation on the function of the SlAVE3, and provides a target for optimizing the antiviral activity of the SlAVE3 through a gene editing technology. The excellent haplotype SpAVE3 derived from wild tomatoes is explored, the ToCV resistance of the excellent haplotype SpAVE3 is obviously superior to that of SlAVE3 of cultivated tomatoes, and a precious gene resource is provided for disease-resistant breeding of tomatoes.

This invention discloses a method for improving crop tolerance to high-temperature stress, belonging to the field of genetic engineering technology. The method includes using biological techniques to enhance the tolerance of OsUVR8b... S177A Genes are upregulated in crop plants to enhance their tolerance to heat stress. This invention provides a gene, OsUVR8b, that can regulate crop tolerance to heat stress. S177A OsUVR8b exhibits natural variation among rice varieties. In heat-sensitive varieties, the 177th position of OsUVR8b is serine, while in heat-resistant varieties, it is alanine. This variation is utilized in rice varieties where OsUVR8b... S177A Gene overexpression can significantly improve the survival rate and seed setting rate of plants under high temperature conditions. This invention provides a molecular basis for breeding and screening heat-resistant varieties. Since this type of variation site exists in nature, it has important practical application value in the future breeding of heat-resistant rice varieties.

The present invention relates to a maize gene ZmRAVL1 and a functional site and use thereof. The present invention locates the functional site for controlling the leaf angle phenotype to 240 bp by fine-mapping, and the insertion and deletion of this region lead to different leaf angle phenotypes. The present invention demonstrates that an inbred line improved with an excellent natural variation from the teosinte can increase maize yield under dense planting and broaden the source of elite alleles available in plant breeding. The present invention demonstrates that a reduced ZmRAVL1 expression by a genetic engineering technique (RNAi) has an influence on plant architectures, such as a reduced leaf angle, and thus it provides excellent genetic resources for genetic engineering breeding. The present invention produces favorable alleles by adopting the gene-editing technology, and thus greatly shortens the selection process of elite alleles, which provides a new idea for obtaining elite alleles available in the breeding practice. The present invention can quickly and accurately improve or produce superior inbred lines with the aid of the molecular marker-assisted selection technology, which provides the possibility of wide application of elite alleles.

The application discloses a functional molecular marker of a wheat TaUFD1-3B gene and application thereof, and belongs to the technical field of crop selection and cultivation. There are 19 SNP variations in the promoter region of the wheat TaUFD1-3B gene, 52 SNP variations and 5 InDel variations in the gene region, the functional molecular marker is a KASP marker, and is directed to a SNP 40 site variation and can be amplified by a forward primer shown in SEQ ID NO: 3 and two reverse primers shown in SEQ ID NO: 4 and SEQ ID NO: 5. The KASP marker developed in the application is derived from natural variation analysis of the TaUFD1-3B gene in a natural population of wheat, provides an effective molecular marker for genetic improvement of flag leaf length and thousand-grain weight of wheat, and can accelerate the breeding process.

The invention discloses application of a rice gene GL6.1 in grain shape and yield breeding, and belongs to the technical field of plant gene breeding application. According to the invention, a new gene GL6.1 for regulating and controlling the grain length of rice is cloned by utilizing a natural variation group. GL6.1 is knocked out by using a CRISPR / Cas9 technology, so that rice grains are remarkably lengthened, and the thousand grain weight is remarkably increased; overexpression of the GL6.1 results in significant shortening of rice grains. The action mechanism of the GL6.1 is further elaborated, cloning and functional research of the gene are beneficial to further understanding of the molecular mechanism of rice grain shape gene regulation and control, the gene can be directly applied to production practice, good gene resources and design elements are provided for molecular design breeding of rice grain shapes, and the gene has high application value in breeding.

A SEC12-like protein gene CPU1 and application thereof in improving soybean phosphorus efficiency are disclosed. Through genome-wide association studies, a major genetic locus affecting soybean phosphorus efficiency is identified, and the candidate gene CPU1 is discovered and validated. There are natural variations in gene CPU1 in soybean population, including two alleles, phosphorus-inefficient allele CPU1-H1 and phosphorus-efficient allele CPU1-H2. Studies based on CPU1-transformation plants shows that inhibiting the expression of the allele CPU1-H2 significantly reduces soybean phosphorus efficiency, and ultimately reduces the biomass and yield of transgenic plants. The present disclosure provides new scientific insights into genetic bases underlying natural phenotypic variation in crops, and provides novel allele resources for molecular breeding of phosphorus efficiency.

This invention discloses a computer device and method for mining stress-responsive variant sites or molecular QTLs based on cross-conditional comparative analysis. First, the invention transforms molecular traits using Bray-Curtis distance, ensuring that the transformed molecular traits represent the influence of natural variation on the molecular traits, including the genetic effects of natural variation and stress response effects. Then, comparative PCA is used to perform dimensionality reduction on molecular traits under stress treatment and those under control, thereby accurately obtaining the stress response effect of the molecular traits and using it as a stress response index. Finally, genome-wide association analysis of the stress response index is used to obtain stress-responsive molecular QTLs for the identification of cross-conditional molecular response QTLs such as those related to stress stimuli.

The invention discloses a functional molecular marker of a wheat TaUFD1-3B gene and application of the functional molecular marker, and belongs to the technical field of crop seed selection and cultivation. 19 SNP variations exist in a promoter region of the wheat TaUFD1-3B gene, 52 SNP variations and 5 InDel variations exist in a gene region of the wheat TaUFD1-3B gene, the functional molecular marker is a KASP marker, and aiming at SNP 40 site variation, the functional molecular marker can be obtained by amplifying a forward primer as shown in SEQ ID NO: 3 and two reverse primers as shown in SEQ ID NO: 4 and SEQ ID NO: 5. The KASP marker disclosed by the invention has the beneficial effects that the developed KASP marker is derived from natural variation analysis of the TaUFD1-3B gene in a wheat natural population, an effective molecular marker is provided for genetic improvement of flag leaf length and thousand seed weight of wheat, and a breeding process can be accelerated.

The invention discloses a method for realizing plant regeneration through hybrid embryo rescue of cymbidium faberi, and belongs to the field of reproductive biology. The cymbidium faberi with novel floral leaf characters is high in price due to extremely high ornamental value, but the varieties and the quantity of the cymbidium faberi with the novel floral leaf characters are rare due to dependence on natural variation and adoption of a division propagation mode, and the collection pressure of the wild cymbidium faberi is caused. According to a hybrid in-vitro embryo rescue technology, parents with novel characters can be combined, regenerated plants of hybrid embryos are obtained through the embryo rescue technology, selectable hybrid progenies with novel phenotypes are provided, and the breeding period is shortened in a nutritional line breeding mode.

This application relates to a CAPS molecular marker for the potato long-day adaptation gene StCDF1.6 and its application. Although four alleles of StCDF1 are currently known, it is still necessary to explore new natural variations to enrich the resource selection for potato breeding in order to cope with the challenges of rapidly changing climate and environment.