SNP locus related to wheat spikelet number per spike and application thereof

A spikelet number and locus technology, applied in the field of SNP loci, can solve the problems of small contribution rate of phenotype, poor repeatability, difficulty in applying wheat genetic improvement, etc.

Active Publication Date: 2019-08-30
中国科学院遗传与发育生物学研究所农业资源研究中心
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Problems solved by technology

However, due to the small phenotypic contribution rate of most of these QTLs and poor reproducibility in different year

Method used

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  • SNP locus related to wheat spikelet number per spike and application thereof
  • SNP locus related to wheat spikelet number per spike and application thereof
  • SNP locus related to wheat spikelet number per spike and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 SNP and its PCR-enzyme polymorphism detection related to the number of spikelets per ear of wheat

[0027] 1. Specific primers and sequence analysis for amplifying the genomic fragment containing the SNP in wheat

[0028] A SNP was found in the gene WFZP promoter region on the wheat genome, corresponding to the 1501st position from the 5' end of Sequence Table 1; there are two genotypes at this site in the wheat natural variation population:

[0029] Genotype A: A

[0030] Genotype B: G

[0031] According to the sequence differences of different genomes of wheat, specific primers were designed to PCR amplify the DNA fragments respectively including the SNP site:

[0032] F1: GCAGGCATCTTTTACACCATCTTA

[0033] R1: GGCAGAAGTGAAGTGAGGT

[0034] F2: GCAAGGATTGTGGCATGCA

[0035] R2: CAAAGAGATGAGGAGAATGTTGAG

[0036] Using F1 and R1 as primers for the target sequence of PCR amplification as shown in the sequence sequence 1 of the sequence listing 1083-1887 sequ...

Embodiment 2

[0059] In 2015, the above-mentioned natural group wheat was planted in the dry land of the Experimental Farm of the Institute of Crop Science of the Chinese Academy of Agricultural Sciences (Shunyi, Beijing), and in 2016 in the dry and wet land of the Experimental Farm of the Institute of Crop Science of the Chinese Academy of Agricultural Sciences (Shunyi and Changping, Beijing). The number of spikelets per ear of each wheat variety is associated with the situation of the number of spikelets per ear and the polymorphic site with Tassel2.1 software, and the mixed linear model+population structure (MLM+(Q+K)) is selected Methods for analysis, with P<0.05 as the significance level, the results are shown in Table 2.

[0060] Table 2 Association analysis results of WFZP-A gene polymorphisms and number of spikelets per panicle in natural populations

[0061]

[0062]

[0063] The results of the association analysis in Table 2 showed that the difference in the number of spikel...

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Abstract

The invention discloses an SNP locus related to wheat spikelet number per spike and application thereof. Through genetic variation analysis of a WFZP-A gene promoter region in a wheat natural variation population, the invention finds an SNP corresponding to 1501st site at the 5'end of a sequence table 1. The SNP has two genotypes: genotype A (A) and genotype B (G). The correlation analysis shows that in the homozygous types of the two genotypes, the spikelet number per spike is as follows: the number of genotype A-homozygous wheat is larger than that of genotype B-homozygous wheat. The invention also provides a dCAPS marker for detecting the SNP. Experiments prove that the wheat with higher spikelet number per spike can be found by detecting the SNP. The invention provides a new method formolecular marker-assisted selection breeding of wheat, and has important significance in the breeding or research high-yield wheat varieties.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a SNP site related to the number of spikelets per ear of wheat and an application thereof. Background technique [0002] Wheat (Triticum aestivum L) is one of the most important food crops in the world and in my country. Improving wheat per unit yield is an important way to meet the continuous increase in food demand this year, and it is also a strategic goal to ensure food security. The number of grains per panicle is one of the three major elements of wheat yield per plant, and the number of spikelets per panicle is the key factor affecting the number of grains per panicle. Therefore, mining the excellent allelic variation that regulates spikelet number per panicle and developing functional markers has important application value in wheat spikelet number improvement and wheat high-yield breeding. [0003] At present, researchers have identified a large nu...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13Y02P60/87
Inventor 刘西岗李永鹏赵美丞郭鑫鑫景蕊莲
Owner 中国科学院遗传与发育生物学研究所农业资源研究中心
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