4 results about "Variation (Genetics)" patented technology

The invention belongs to the technical field of animal husbandry genetic breeding, and particularly relates to a mitochondrial SNP molecular marker related to egg laying performance of laying hens and application of the mitochondrial SNP molecular marker. The invention provides a mitochondrial SNP (Single Nucleotide Polymorphism) molecular marker related to the egg laying performance of laying hens, which is positioned at the 15944th bp of a chicken mitochondrial genome and has C / A / T polymorphism. The mitochondrial genome has maternal hereditary characteristics, systematic mitochondrial whole genome hereditary variation analysis is carried out to verify the genetic effect of the laying hen breeding trait mitochondrial gene, and the poultry genetic breeding theory is supplemented and perfected; moreover, a novel extranuclear genetic molecular marker and a variety breeding method are established, and the method has practical application value and important scientific significance.

The invention provides a probe composition, a gene chip, a reagent, a kit and application. The probe composition is designed based on capture areas of 34 genes related to dominant single-gene genetic diseases, can be used for non-invasive prenatal genetics screening, and is suitable for prenatal screening of genetic variation positive family history, bad fertility history, fetal ultrasound examination abnormality, pregnant woman elderly, father elderly and the like. The omission ratio and the birth rate of fetuses suffering from the dominant single-gene hereditary disease are effectively reduced.

The invention discloses a screening method for polymorphic genetic markers of livestock disease resistance characters and application, and relates to the technical field of animal molecular genetics and genome breeding. Disease-resistant key cell subgroups are located through single cell transcriptome sequencing, eQTL analysis of the purified subgroups is specifically carried out, and the disease-resistant key cell subgroups are obtained. The method is used for overcoming the defect that a marker is lack of cell type specificity due to dependence on a mixed sample, immune cells are grouped through single cell transcriptome sequencing, key cell subgroups in disease-resistant response are screened out in combination with hub scoring, the subgroups are purified by adopting a cell sorting technology, it is ensured that subsequent eQTL analysis is only carried out for the core subgroups, and the result is accurate. The process can clearly distinguish specific cell types with heritable variation effects, so that the screened eQTL marker is directly associated with a disease-resistant regulation mechanism, accurate correspondence of the marker and cell functions is realized, interference of a mixed sample on marker specificity is avoided, and the marker has clear cell type specificity and function directivity.

This invention discloses a method and application for screening polymorphic genetic markers of disease resistance traits in livestock, relating to the fields of animal molecular genetics and genome breeding technology. This invention uses single-cell transcriptome sequencing to locate key cell subpopulations for disease resistance, and then conducts targeted eQTL analysis on these purified subpopulations. This addresses the shortcomings of relying on mixed samples, which leads to a lack of cell type specificity in markers. Immune cells are grouped using single-cell transcriptome sequencing, and key cell subpopulations in the disease resistance response are screened using pivotal scoring. Cell sorting technology is then used to purify these subpopulations, ensuring that subsequent eQTL analysis is conducted only on these core subpopulations. This process clearly distinguishes the specific cell types involved in the genetic variation, enabling the screened eQTL markers to be directly associated with disease resistance regulatory mechanisms. This achieves a precise correspondence between markers and cell functions, eliminating the interference of mixed samples on marker specificity and giving the markers clear cell type specificity and functional targeting.