Cotton breeding method

A cotton variety, cotton technology, applied in the field of crop breeding

Inactive Publication Date: 2010-05-19
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also some reports that mutations can be produced by grafting, among which there are many reports on chimeras generated by grafting and genetic variation induced by grafting, but whether the exchange of genetic material occurs is still academically debated

Method used

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  • Cotton breeding method
  • Cotton breeding method
  • Cotton breeding method

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Experimental program
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Embodiment 1

[0028] Embodiment 1 A kind of cotton breeding method, comprises the steps:

[0029] (1) Cultivate grafted seedlings

[0030] ①Cultivating rootstocks Select the non-transgenic cotton Zhongmiansu 24 with improvement potential as rootstocks, sow them in seedbeds or nutrient bowls, and breed them into rootstocks;

[0031] ②Cultivate scion plants Select the cotton variety Zhongmiansuo 41 transfected with bivalent insect-resistant genes Bt+CpTI as scions, sow them in seedbeds or nutrient pots, and breed them into scion plants;

[0032] ③Grafting When the rootstock and the scion have 1 to 2 true leaves, graft by grafting: use a sharp blade to remove the top bud on the cotyledon of the rootstock, keep a cotyledon on the main stem, and move along the main stem of the rootstock. Split the inclined plane about 1.5cm long from top to bottom; then remove the cotyledons on the lower root of the scion plant and the stem, retain the growth point of a true leaf and the main stem with a length...

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Abstract

The invention relates to a cotton breeding method. In the method, a combination part of genetic variation generated by cotton graft is used as an explant for inducing to culture callus; the cultured callus is subjected to propagation, subculturing and differentiated culture to obtain cotton seedlings; and after hardening off, the cotton seedlings are grafted for transplantation or directly transplanted. The cotton breeding method has the advantages that: by utilizing graft to create genetic variation, the method not only improves plant quality, yield, stress resistance, and the like, but also obtains distant variation which cannot be obtained through sexual hybridization, and has simple operation, high seed selection efficiency and low cost; the method provides a novel cotton transgenic means and can transfer target gene to a breed to be improved to generate a variation type combining the merits of parental stock and cion parents; and the creation of genetic variation by utilizing graft can be taken as a supplementary means of general breeding and modern gene engineering breeding and is an important breeding means with broad prospects.

Description

technical field [0001] The invention relates to the field of crop breeding, in particular to a cotton breeding method. Background technique [0002] The basic process of crop breeding is the steps of creation of variation-selection of mutated offspring-determination of superior genotype-identification and reproduction. As far as the whole process is concerned, the most basic step is to create variation first. Based on different methods of creating variation, the current breeding methods can be summarized as follows: ①Mutation breeding refers to the breeding method of obtaining new biological varieties by artificial mutagenesis (physical mutagenesis or chemical mutagenesis). Advantages: Increase the mutation frequency and wide range of mutations. Disadvantages: less beneficial mutations, a large amount of materials must be processed; the direction and nature of the mutagenesis cannot be controlled; ② hybrid breeding refers to the use of biological individuals with different g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G1/06A01H4/00
Inventor 郝俊杰
Owner HENAN ACAD OF AGRI SCI
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