59results about How to "Improve vaccination success rate" patented technology

The invention relates to a method of tissue culturing and fast breeding pinellia tuber of toxicity-removing, comprising the following steps: employing the immature seed and /or mature seed of pinellia tuber as explant, breeding large quantity of tissue culturing sprout of toxicity-removing, bulbil of toxicity-removing and small root tuber of toxicity-removing after evoked culturing, enrichment culturing, root growing culturing, replanting and virus detection. The invention is characterized by the simple inoculation, low inoculation pollution rate, high survival rate; simple, fast and through toxicity removing; 5-10 times breeding coefficient, fast breeding, suitable for factory breeding and commercial production.

The invention relates to an inoculator of edible fungus, comprising a steam pressure tank, a gas compressor, two gas filters in series, a gas distributor and a fungus seed storage tank; the inoculator adopts steam generated by the steam pressure tank to carry out sterilization to a pipeline system, and utilizes the gas compressor, the gas filters and the gas distributors to form sterile operating environment so as to implant fungus packs in liquid spawn or colloid spawn in the fungus seed storage tank of the confined steam pressure tank to realize the quantitative inoculation. The invention has the advantages of convenient operation, fast speed, consistent inoculation, no pollution, high efficiency with greatly reducing labor intensity, etc., which is applicable to industrial production and countries of mountainous areas.

The invention discloses edible fungi sterilization and inoculation integrated equipment, comprising a liquid strain fermentation tank and a sealed sterilization warehouse. The external part of the sterilization warehouse is provided with a steam intake pipe, a steam discharge pipe, a water intake pipe, a water discharge pipe, a strain pipe, an air intake pipe, an air discharge pipe, an air filterand a fan; the internal part of the sterilization warehouse is provided with a plurality of layers of supporting frames; water circulating pipes which are arranged in a bending way are arranged on each layer of supporting frames; two ends of the water circulating pipes are respectively connected with the water intake pipe and the water discharge pipe; a plurality of auxiliary inoculation pipes arearranged between adjacent water circulating pipes; the end parts of the auxiliary inoculation pipes are provided with primary inoculation pipes; and the strain pipe is communicated with the liquid strain fermentation tank. The edible fungi sterilization and inoculation integrated equipment has simple structure, reduces the output links, is cooled without the requirement of going out of a pan, hasautomatic inoculation and high inoculation success rate, avoids the pollution brought by the interference of artificial factors, and essentially realizes automation, stabilization and standardization, thereby achieving the purposes of labor and time conservation as well as low pollution rate, and being the equipment with very high degree of automation.

The invention discloses a classical swine fever virus auxiliary fluid for inoculating a rabbit and a preparation method thereof, and belongs to the technical field of biological products for livestock. Every 1000ml of phosphate buffer solutions in the auxiliary fluid comprises the following components in proportions by weight: 6g to 10g of vitamin compositions, 3g to 5g of amino acid compositions, 10g to 30g of compound Chinese medicinal herbal polysaccharides, and 0 to 10mg of sodium selenite. The classical swine fever virus auxiliary fluid for inoculating the rabbit has the positive and beneficial effects that a virus can be delivered to a susceptible organ when the rabbit is inoculated; the inoculation loss is reduced; the inoculation success rate is improved; the infection stability of the rabbit can be improved; batch-to-batch differences are reduced; the batch-to-batch stability of finished-product vaccines is ensured; the quality of the vaccines is improved; the multiplication density of a classical swine fever virus in the body of the rabbit can be specifically improved; in comparison with the prior art, the titer of the virus is increased by 5 to 10 times; the weight of a gained splenic organ is discovered to be slightly increased after the rabbit is inoculated; in comparison with the prior art, the weight of the gained splenic organ is increased by 15 percent to 40 percent.

The invention belongs to the technical field of microbial electrochemistry and discloses a construction method of a microbial electrochemical system for hydrogen phosphide generation reinforcement. The construction method comprises mixing animal wastes and anaerobic activated sludge, adding the mixture into an anaerobic reactor, carrying out culture through a medium to obtain a phosphine-producing microorganism-rich domesticated activated sludge mixed solution, taking the domesticated activated sludge mixed solution, carrying out centrifugation separation, carrying out washing through a phosphate buffer solution, adding inoculation sludge into a microbial electrolysis cell, carrying out biofilm culturing, applying power for the microbial electrolysis cell, adding the electrolysis product into a medium and carrying out anaerobic cultivation for hydrogen phosphide generation reinforcement. Through construction of the microbial electrolysis cell and domestication of microbes, a hydrogen phosphide yield of phosphate reduction and a hydrogen phosphide production rate are improved. The construction method has a good application prospect.

The invention belongs to the technical field of fungal fertilizer and particularly relates to a mycorrhizal fungi microcapsule seed coating agent capable of improving a saline-alkali soil microbial flora structure as well as a preparation method of the mycorrhizal fungi microcapsule seed coating agent. The seed coating agent particularly comprises the following components: 1 to 1.5 parts of arbuscular mycorrhizal fungi preparation, 0.1 to 0.2 part of trichoderma harzianum preparation, 3 to 5 g of xanthan gum, 1 to 1.2 g of calcium carbonate, 0.1 to 0.2 g of citric acid aqueous solution, 0.2 to0.3 g of ethyl lactate and 80 to 100 mg of polylactic acid. The microcapsule is prepared by a mode of forming compound emulsion by the polylactic acid and the xanthan gum; the prepared seed coating agent has high film-forming property and high embedding rate; the formed capsule wall has high stability, can effectively protect the activity of the fungi, can directly coat the seed and has good slow-release effect; through mutual synergistic effect of the arbuscular mycorrhizal fungi and the trichoderma harzianum, the inoculation success rate is high; and after inoculation, the yield of crops isobviously increased and the morbidity probability of the crops is reduced.

The invention discloses a liquid inoculation method for artificially planting cistanche. The liquid inoculation method includes the following steps of cistanche seed grading, screening and germinating treatment, modifying of an inoculating device, inoculating liquid preparing, inoculating ditch excavating, farmyard manure fertilizing, liquid inoculating, inoculating ditch backfilling and conventional irrigation management after watering for the first time and soil moisture filling. The liquid inoculation method has the advantages that by the liquid inoculation method for cistanche seeds, germination, growing and restoring of host root systems can be promoted, distance between the seeds and the host root systems can be shortened, inoculating section can be expanded to increase contact probability of the seeds and host roots, and inoculating success rate and yield are increased while seed consumption is reduced. Experiments show that compared with methods of direct broadcast sowing and seed paper inoculating, the liquid inoculation method is seed saving, convenient for operation, short in inoculation period and highest in inoculation rate, spray gun equipment with diameter of 2.0mm and used in the method is medium in seed spraying quantity and good in spraying effect, seed consumption is reduced due to spraying, and the liquid inoculation method is high in inoculation rate, convenient for operation during production and easy for popularization.

The invention discloses a method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation. A prepared gibberella spore liquid is inoculated into bilateral florets of a sixth spikelet at the top of a wheat ear by utilizing a double-flower instilling method in a wheat flowering stage, a browning condition of inoculated spikelet is recorded, a diseased spikeletrate of infected spikelet at the lower part of the inoculated spikelet after inoculation is counted, and wheat scab extension resistance is dynamically evaluated. According to the inoculation method,a diseased part mainly expands downwards from the sixth spikelet on the top, the diseased spikelet rate at the lower part of the sixth spikelet is counted, diseased parts are consistent, the problem that identification reliability is affected due to the fact that white spikes are prone to being withered through a traditional single-flower instillation method is solved, and the extension resistanceof varieties can be reflected more accurately.

The invention discloses a method for measuring pathogenicity of pathogen wood-rotting fungi in basidiomycetes by standing timber stems. The method includes the steps: (1) manufacturing a wood dust culture medium containing 82% of wood dust, 15% of wheat bran, 2% of glucose and 1% of gypsum; (2) introducing bacterial strains into the wood dust culture medium and manufacturing an inoculant stick; (3) selecting a young tree, disinfecting the young tree, cutting a stem 10-30cm away from the ground to form a 1-5cm long and 1-5mm deep notch serving as an inoculation position matched with a shear mouth on one side of the inoculant stick, attaching wet cotton to the lower end of the inoculation position to preserve moisture, fixedly wrapping the inoculation position with a plastic strip, and wrapping the stem about 5-10cm away from the upper portion of the inoculation position with thick paper to form an umbrella to cover the inoculant stick; (4) performing inoculation and infection for 1-2 months, unfastening the thick paper and the plastic strip wrapping an inoculant and verifying the pathogenicity. By the method, the young standing timber stems are inoculated for the first time, the pathogenicity of the pathogen wood-rotting fungi causing tree stem rot and root rot is measured, success rate is high, and operation is simple.

The invention provides a separation and purification method of eimeria tenella sporozoite and an inoculation method, and relates to the technical field of biology. The separation and purification method comprises the following steps: taking infected chicken manure, adding water, and filtering; centrifuging, adding a saturated saline solution into the precipitate, centrifuging, and adding water into the supernate to obtain an oocyst diluent; centrifuging, and adding chloramine T solution into precipitate to obtain sporulated oocyst suspension; centrifuging, adding saturated saline solution into the precipitate for resuspending, centrifuging and collecting oocyst precipitate; adding a sodium hypochlorite solution for resuspension, and collecting oocysts; and shaking and crushing, stopping shaking when all sporangia escapes, centrifuging to obtain sporangia, adding pancreatin digestive juice for digestion, filtering, centrifuging, and collecting precipitate to obtain eimeria tenella sporozoites. According to the inoculation method, the sporangiums or sporozoites are accurately injected into the middle sections of muscular stomachs and small intestines of the chickens, and successful infection is achieved. The purification degree of the sporangiums and sporozoites obtained by the purification method is high, the experimental operation is simple and convenient, and the infection success rate after injection inoculation reaches 100%.

The invention relates to a sterilization device used in shiitake mushroom industrial production. The device comprises a sterilization chamber. A first sealing door and a second sealing door are separately arranged at the two ends of the sterilization chamber. The second sealing door is directly positioned in an inoculation workshop. The sterilization chamber is separately provided with a steam inlet pipe and a steam discharge pipe. A gas distribution pump is arranged in the ground of the sterilization chamber. The outlet of the steam inlet pipe is positioned in the gas distribution channel. A plurality of gas distribution holes are formed in the top of the gas distribution channel. An exhaust valve is arranged on the steam discharge pipe. According to the invention, the second sealing door which serves as the outlet of the sterilization chamber is directly arranged in the inoculation workshop, such that fungus cylinders are prevented from being contaminated by hybrid fungi in air during a transport process after sterilization. Therefore, inoculation success rate is improved, and subsequent inoculation operation steps are reduced. With bottom gas distribution and sealed pressurization sterilization manners, and in combination with a post-stage active heat discharge manner, sterilization time is shortened, and production efficiency is improved. The device is suitable for shiitake mushroom industrial production.

The present invention discloses a hepatitis B vaccine vaccination prevention and control method of rural children, the method is characterized by comprising psychological intervention on an object of vaccination, sequence vaccination of hepatitis B vaccine, a return visit for monitoring after the implementation of the vaccination and interference processing of vaccination problems, hepatitis B vaccine vaccination epidemic prevention of children can be improved, scruples of the object of vaccination and a guardian can be eliminated, the success rate of the vaccination can be improved, and improvement of hepatitis B vaccine vaccination prevention and control of the rural children and the quality of service can be facilitated.