Method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation

Abstract

The invention discloses a method for identifying wheat scab extension resistance through wheat ear top double-flower inoculation. A prepared gibberella spore liquid is inoculated into bilateral florets of a sixth spikelet at the top of a wheat ear by utilizing a double-flower instilling method in a wheat flowering stage, a browning condition of inoculated spikelet is recorded, a diseased spikeletrate of infected spikelet at the lower part of the inoculated spikelet after inoculation is counted, and wheat scab extension resistance is dynamically evaluated. According to the inoculation method,a diseased part mainly expands downwards from the sixth spikelet on the top, the diseased spikelet rate at the lower part of the sixth spikelet is counted, diseased parts are consistent, the problem that identification reliability is affected due to the fact that white spikes are prone to being withered through a traditional single-flower instillation method is solved, and the extension resistanceof varieties can be reflected more accurately.

Description

technical field [0001] The invention relates to a method for identifying extended resistance to wheat scab by inoculating double flowers at the top of wheat ears, and belongs to the technical field of identification and evaluation of plant disease resistance. Background technique [0002] At present, the main method used in the identification of extended resistance to scab is the single flower drip method, that is, the single floret of the middle and lower spikelets of wheat ears is injected with spore liquid at the flowering stage of wheat, the inoculation time is recorded, and 18-18 days after inoculation. The rate of diseased spikelets was counted in 21 days, that is, the percentage of diseased spikelets to the total number of spikelets was evaluated for disease resistance. The traditional single-flower instillation method inoculates spikelets in the middle and lower parts of the spikelets, and the upper part of the spikelets is often damaged due to the destruction of vas...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problems of withered and white spikes and the poor consistency of identification results that are prone to occur in the traditional single-flower instillation method, and to provide a kind of inoculated spikelet position with clear location, high inoculation success rate, obvious difference in resistance and susceptibility phenotype, and low incidence of disease. A method for identification of extended resistance to head blight with good consistency