Culture method of liquid strain-solid cultivation species of straw rotting edible fungi

A technology of solid cultivation and liquid strain, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of high energy consumption, few types of medicinal residues, and low degree of industrialized utilization, so as to improve the degree of automation and shorten the fermentation cycle. , the effect of shortening the processing cycle

Inactive Publication Date: 2018-12-25
重庆市药物种植研究所
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Problems solved by technology

However, there are rare reports on the utilization of liquid strains in the utilization of traditional Chinese medicine dregs
And based on the following reasons, the resource utilization of traditional Chinese medicine dregs is lagging behind, which is not enough to guide production and application: ①There are many types of traditional Chinese medicines and complex prescriptions, resulting in many types of medicinal dregs, and current research or application is only carried out on a certain kind of medicinal dregs , the degree of industrial utilization is low; ②The conversion rate of traditional Chinese medicine dregs to organic fertilizers is about 50%, and the amount of traditional Chinese medicine dregs in most pharmaceutical manufacturers is between several hundred tons to several thousand tons, which is not suitable for the construction of large-scale organic fertilizer plants; ③Generally used as There are few types of dregs of feed or feed additives, which can only be used as an auxiliary treatment method
④ When using traditional Chinese medicine dregs to cultivate edible and medicinal fungi, solid strains are mainly used, which has low utilization efficiency and is not conducive to popularization and application
⑤Using traditional Chinese medicine dregs to ferment hydrogen and biomass gasification technology to produce biomass energy has great potential and can realize high value-added transformation. In the research and development stage, there is no large-scale application demonstration base in China
[0005] Therefore, a method for resource utilization of traditional Chinese medicine dregs is developed to solve the problem that small and medium-sized medicine dregs enterprises are not convenient for resource utilization

Method used

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  • Culture method of liquid strain-solid cultivation species of straw rotting edible fungi

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1 Chinese medicine residue cultivar formula

[0055] Formula 1 of traditional Chinese medicine dregs cultivar: by weight, take by weighing the main ingredients 45 parts of perilla dregs, 40 parts of Jianpi Zhixiening dregs; auxiliary materials: 5 parts of peanut shells, 5 parts of wheat bran, 3 parts of corn flour, 0.925 parts lime, 1 part gypsum, 0.05 parts KH2PO4, 0.025 parts MgSO4.

[0056] Formula 2 of traditional Chinese medicine dregs cultivars: weigh 90 parts of the main ingredient, 90 parts of emergency syrup dregs by weight; auxiliary materials: 5 parts of wheat bran, 3 parts of corn flour, 0.925 parts of lime, 1 part of gypsum, 0.05 parts of KH2PO4, 0.025 parts of MgSO4.

[0057] Formula 3 of traditional Chinese medicine dregs cultivar: by weight, weigh 45 parts of Daqingye Banlangen medicinal dregs and 40 parts of rhizome medicinal dregs as main ingredients; auxiliary materials: 5 parts of wheat bran, 3 parts of corn flour, 0.925 parts of lime, 1 ...

Embodiment 2

[0060] Embodiment 2 Chinese medicinal residues cultivate straw mushroom

[0061] (1) Activation of Straw Mushroom strains

[0062] Pick fresh strain and inoculate in PDA enriched medium (formula: potato 200g / L, agar 20g / L, glucose 20g / L, vitamin B1 2 grains / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.25 g / L, natural pH. Sterilize at 121°C for 30min, make a slope, and inoculate after standing for 2 days). Straw mushroom cultivation temperature is 33 ℃, cultured for 4-7 days, it can be used for primary seed inoculation;

[0063] (2) Production of primary seed medium

[0064] a. Preparation of culture medium: Accurately weigh 20g of glucose, 4g of peptone, 3g of yeast extract, 0.5g of dipotassium hydrogen phosphate, 0.25g of magnesium sulfate, and 2 capsules of vitamin B1, dissolve them in 1L of distilled water, melt in an induction cooker, and set the volume to 1L, adjust pH 5.8~6.0.

[0065] b. Sterilization of the culture medium: After the culture medium ...

Embodiment 3

[0087] Embodiment 3 Cultivation of chicken leg mushrooms with Chinese medicinal residues

[0088] (1) Production of liquid culture medium

[0089] a. Preparation of liquid primary culture medium

[0090] Liquid culture medium: accurately weigh 20g of glucose, 4g of peptone, 4g of yeast extract, 0.5g of dipotassium hydrogen phosphate, 0.25g of magnesium sulfate, 2 capsules of vitamin B1, dissolve in 1L of distilled water, melt in an induction cooker, and adjust the pH to 5. 8 to 6.0.

[0091] b. Subpackage and sterilization of culture medium

[0092] After the culture solution is prepared, put it into a 1L conical flask, the volume of each bottle is 500mL, and add 20 to 30 glass beads, add cotton plugs and then wrap the kraft paper to seal, at 1.5kg / cm 2 Sterilize under pressure for 30 minutes, remove and cool.

[0093] c. Inoculation

[0094] Inoculation needs to be done on a clean bench. Before inoculation, the inoculation room should be sterilized by ultraviolet lamp, ...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine biology, and particularly relates to a culture method of liquid strain-solid cultivation species of straw rotting edible fungi. The culture method comprises the specific steps of liquid strain propagation, formula and preparation of the solid culture species of traditional Chinese medicine residues, fermentation of cultivation materials, greenhouse cultivation and the like. The culture method has the advantages that the resource utilization of the traditional Chinese medicine residues is combined with the liquid strain, the culture method is more suitable for the large-scale utilization of the traditional Chinese medicine residues, the fermentation cycle can be effectively shortened, the survival rate of the edible fungi is improved, and the utilization efficiency of the medicine residues is improved. A feasible method is provided for the resource utilization of the traditional Chinese medicine residues.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine, and in particular relates to a method for culturing a liquid strain-solid cultivar of grass-rot edible fungi. Background technique [0002] Compared with solid strains, liquid strains have the advantages of short production cycle, fast bacteria growth, neat bacterial age, and easy mechanized operation. With the development of large-scale production of edible fungi, the use of liquid culture technology to prepare liquid strains instead of traditional solid strains will be an important direction for the development of edible fungi production. Due to the high requirements for production technology and technical conditions of liquid bacteria, and the limitation of funds and technology, there are still quite a few edible fungi production enterprises in my country that adopt the traditional solid bacteria cultivation mode. [0003] Traditional Chinese medicine slag is the waste ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/40A01G18/20
CPCA01G18/20A01G18/40
Inventor 雷美艳朱吉彬竭航蒲盛才杨天建张万超李娜
Owner 重庆市药物种植研究所
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