56 results about "Industrial utilization" patented technology

The invention discloses a genetically engineered bacterium and an application of the genetically engineered bacterium in production of cis-3-hydroxy-L-proline. According to the genetically engineered bacterium, a proline dehydrogenase gene putA and a proline transporter gene putP in a starting bacterium are silenced, and meanwhile, a gamma-glutamyl kinase mutant gene proBD107N, a gamma-glutamyl phosphate reductase gene proA and a cis-3-hydroxy-L-proline hydroxylase gene P3H are subjected to overexpression. And by comparing a plurality of common escherichia coli, the cis-3-hydroxy-L-proline hydroxylase gene P3H and different gamma-glutamyl kinase mutant genes, the highest yield combination is preferably selected. The strain takes glucose as a raw material, so that the production cost is greatly saved, and the strain has a relatively good industrial utilization value.

The invention provides a self-assembled cross-linked enzyme aggregate based on a protein scaffold and a preparation method of the self-assembled cross-linked enzyme aggregate, and belongs to the technical field of biology. The preparation method comprises the following steps: connecting an enzyme connected with SpyTag to a SpyCatcher protein scaffold to form a self-assembled cross-linked enzyme aggregate; the amino acid sequence of the SpyCatcher protein is as shown in SEQ ID NO: 1. According to the self-assembled cross-linked enzyme aggregate prepared by the invention, the conformation and the enzyme activity of the enzyme are reserved to the maximum extent, meanwhile, the thermal stability and the reusability are improved, and a foundation is laid for subsequent industrial utilization.

The invention relates to lignin-doped montmorillonite hydrogel as well as a preparation method and application thereof, and belongs to the field of functional materials. A preparation method of lignin-doped montmorillonite hydrogel comprises the following steps: sequentially adding montmorillonite k-10, methacryloyloxyethyl trimethyl ammonium chloride, acrylic acid, acrylamide, N, N-methylene bisacrylamide and potassium persulfate into an aminated lignin aqueous solution, uniformly stirring, carrying out a free radical polymerization reaction, soaking the obtained product in deionized water, and drying to obtain the lignin-doped montmorillonite hydrogel. The lignin doped montmorillonite hydrogel is obtained. The method for preparing the lignin-doped montmorillonite hydrogel is a one-step preparation method, the process is simple, the production cost is low, and the lignin-doped montmorillonite hydrogel is more suitable for large-scale industrial utilization. The lignin-doped montmorillonite hydrogel prepared by the preparation method disclosed by the invention contains rich active sites, has relatively strong adsorption capacity on nitrogen and phosphorus ions and micro-plastics, and can adsorb the nitrogen and phosphorus ions and the micro-plastics at the same time.

The invention discloses a fly ash-based early-strength curing agent as well as a preparation method and application thereof, and belongs to the technical field of building materials. The fly ash-based early-strength curing agent comprises the following components in percentage by mass: 55-65% of low-calcium fly ash, 8-10% of cement, 3-5% of slag, 3-5% of lime, 3-6% of phosphogypsum, 2-4% of a strong oxidant and 8-10% of an alkali activator. The fly ash-based early-strength curing agent is used for soil reinforcement in foundation pit engineering. A deep mixing pile reinforcing technology is adopted for the coal ash-based early-strength curing agent, the coal ash-based early-strength curing agent is fully mixed with soil to be reinforced in a guniting mode, and the doping weight of the early-strength curing agent is 5%-20% of the weight of the soil to be reinforced. According to the preparation method provided by the invention, industrial waste residues such as fly ash are used for replacing or partially replacing the cement curing agent, so that the industrial waste residues are fully utilized, the industrial utilization rate is improved, the early strength of soil curing can be improved, and the problems of serious resource consumption and CO2 emission easily caused by a traditional Portland cement curing agent are solved.

The invention relates to a coal gangue-based glass fiber and a preparation method thereof, belongs to the technical field of comprehensive utilization of solid wastes, and solves the technical problems of high additional value and industrial utilization of coal gangue, and adopts the technical scheme that the coal gangue-based glass fiber comprises the following components in parts by weight: 10 parts of coal gangue; 1-3 parts of quartz sand; 1-2 parts of dolomite; 0.1 to 0.5 part of an additive; the preparation method of the coal gangue-based glass fiber comprises the processes of raw material crushing, coal gangue-based powder pretreatment, mixing and molding, two-step heating and melting, wire drawing and molding, cooling and fiber forming, and sizing and drying. The preparation method has the advantages of wide raw material source, low cost, simple preparation process, easy realization of industrial production, excellent performance of the obtained glass fiber and the like, and the successful implementation of the preparation method not only can realize effective utilization of the coal gangue, but also can provide a new raw material source and a new process route for the glass fiber industry; the method has important economic and social significance.

The invention discloses a method for leaching valuable elements in silicate rare earth ore, and belongs to the technical field of rare earth metallurgy. According to the method, the valuable elements in the rare earth concentrate are efficiently leached by adopting the processes of low-temperature sulfating intensified roasting, clinker hydrometallurgy, leached ore pulp flocculation and filter cake washing. The strengthened low-temperature sulfating roasting process avoids the problems of high energy consumption, serious equipment corrosion, low rare earth leaching rate and complex waste gas treatment (SO2 escape) of a traditional process, meanwhile, silicate rare earth ore decomposition is achieved, the effect of a back-end leaching process is remarkably improved, compared with an acid pickling process, the hydrometallurgy process reduces the acid consumption and the wastewater treatment difficulty, and the production cost is reduced. The leaching ore pulp flocculating settling process is beneficial for improving the industrial production efficiency, the filter cake washing process is beneficial for further improving the leaching rate of valuable elements, the silicate rare earth ore concentrate resources are utilized to the maximum extent on the premise of being environmentally friendly, and a technical path is provided for industrial utilization of silicate type rare earth ore.

The invention relates to an extractive distillation device and a waste liquid recovery method. The extractive distillation device comprises an evaporator, a primary tower, an impurity removal tower, an extractive distillation tower and a product recovery tower which are sequentially communicated through pipelines; the evaporator is provided with a raw material inlet, a first liquid phase inlet, a first liquid phase outlet, a first gas phase outlet, a first steam inlet and a first steam condensate outlet, and the primary distillation tower is provided with a primary distillation gas phase inlet and a primary distillation liquid phase outlet; the first gas phase outlet is communicated with the primary distillation gas phase inlet through a pipeline, the first liquid phase inlet is communicated with the primary distillation liquid phase outlet through a pipeline, and the first liquid phase outlet is used for flowing out impurities. According to the device and the recovery method, MDG and NMF in the waste liquid are further separated by adopting the extraction agent, MDG and NMF products can be respectively recovered, and thus the industrial utilization value of the MDG and NMF mixture is expanded.

A centrifugal fan with a tongue portion air gap comprises a shell, the shell is provided with a vortex type side plate and an air inlet, the vortex type side plate comprises an outlet cavity and an air outlet, a tongue portion is formed on one side of the air outlet, and the tongue portion comprises an inwards-inclined section, an outwards-inclined section and a tongue flange arranged between the inwards-inclined section and the outwards-inclined section; the centrifugal fan blade is rotationally arranged in the cavity, and one side of the centrifugal fan blade and the tongue part inwards-inclined section are arranged in a spaced mode; and the noise reduction air gap is arranged at the tongue part in a bypass channel form and comprises an air inlet end, an air outlet end and a bent channel between the air inlet end and the air outlet end. According to the centrifugal fan, the intensity of narrow-band noise can be reduced, the effect of greatly reducing the noise at the air outlet is further achieved, and therefore the quality of the centrifugal fan is improved. Compared with the prior art, the utility model has practical advancement and better industrial utilization.

The invention relates to the technical field of corydalis saxicola bunting cultivation, in particular to a method for imitating wild planting of corydalis saxicola bunting in karst rock seams, which comprises the following steps: (1) sowing and seedling raising: adding a water-retaining seedling raising substrate into a non-woven fabric seedling raising bag, and then sowing the treated corydalis saxicola bunting seeds in a hill-seeding manner; thoroughly spraying water once after sowing, and then keeping the substrate wet; the sunshade rate is kept at 60-70%; (2) transplanting and planting: planting the seedlings in karst rock seams with the condition of 60-80% of sunshade rate in a non-bag-removing manner, and covering the bottoms and the peripheries of the bag seedlings with a proper amount of water-retaining bonding nutrient soil during planting; (3) spraying water: spraying root fixing water after planting, and keeping the soil humidity at 50-65%; (4) fertilization: filling a non-woven fabric bag with a slow-release fertilizer to prepare a slow-release fertilizer bag, and directly placing the slow-release fertilizer bag at an uphill position beside the plant during fertilization, so that the fertilizer is slowly released in a raining or water spraying process. The technology provided by the invention can be used for original habitat regression conservation of corydalis saxicola bunting and industrial utilization of traditional Chinese medicinal materials.

The invention relates to a method for simultaneously preparing high-purity carbon dioxide and nitrogen by using flue gas, which is characterized in that flue gas is used as a raw material, and the high-purity carbon dioxide and nitrogen are economically and conveniently obtained by dedusting, removing impurities such as oxygen, hydrocarbon, hydrogen, CO and the like and reasonably utilizing heat of purification treatment. Belongs to the technical field of carbon capture, waste gas treatment and resource utilization. The method mainly comprises the following six parts: fine removal and dust removal, heat exchange and low-pressure steam production, pretreatment, carbon-nitrogen separation, high-purity nitrogen production and high-purity carbon dioxide production. A mature organic amine method is used for carbon-nitrogen separation, a PSA method is used for high-purity nitrogen refining, and a low-temperature liquefaction method is used for liquid carbon dioxide. On the whole, high-purity carbon dioxide and nitrogen which can be used for chemical synthesis and industrial utilization are obtained through deep impurity removal of flue gas, reasonable configuration of the technological process and comprehensive utilization of heat generated by deoxidation, and 0.3 MPa saturated steam lt is supplemented by a carbon-nitrogen separation unit; 0.9 ton / tCO2, and the loss of the organic amine sold in the market is 1t; and 0.5 kg / t of CO2.

The application relates to a preparation method of 2-amino-6-hydroxypyridine compounds and intermediates thereof, and belongs to the field of organic synthesis. The preparation method of the 2-amino-6-hydroxypyridine compounds comprises the following steps: 1) in a first organic solvent, compound II is reacted with a cyclic acid anhydride reagent A to prepare compound III; 2) in a second organic solvent, in the presence of an oxidizing reagent, compound III is subjected to an oxidation reaction to prepare compound IV; 3) in a third organic solvent, in the presence of an acid anhydride dehydrating reagent, compound IV is subjected to a reaction to prepare compound V; 4) in a fourth solvent, in the presence of a reducing agent, compound V is subjected to a reduction reaction to prepare compound I; and compound I is a 2-amino-6-hydroxypyridine compound. The preparation method of the 2-amino-6-hydroxypyridine compounds provided by the application can obtain a higher yield through mild reaction conditions, and is simple and convenient to operate, and is beneficial to industrial utilization.

The invention discloses an iron-cobalt-nickel layered double hydroxide / graphite-alkyne nano composite material and a preparation method and application thereof, and the preparation method comprises the following steps: mixing iron salt, cobalt salt and nickel salt to prepare a precursor solution, adding the precursor solution and an alkaline solution into a graphite-alkyne dispersion liquid, stirring, and carrying out a hydrothermal reaction to obtain the nano composite material. The nano composite material prepared by the invention has the advantages of large specific surface area, good dispersity, multiple reaction active sites, high catalytic activity, good stability and the like, is a novel composite catalyst with excellent catalytic performance and stable structure, can efficiently activate persulfate when being used for activating the persulfate, and has the advantages of simple preparation process, low cost and the like. The constructed degradation system can efficiently degrade various antibiotics, and is high in use value and good in application prospect. Meanwhile, the preparation method has the advantages of being simple in process, convenient to operate, mild in reaction condition, low in cost, environmentally friendly and the like, can be used for large-scale preparation and is convenient for industrial utilization.

This invention discloses an artificial breeding method for *Schizothorax lancensis*, belonging to the field of aquatic animal breeding technology. The artificial breeding method for *Schizothorax lancensis* provided by this invention includes: intensive three-step cultivation of broodstock, injection of spawning induced agents after cultivation, artificial fertilization, hatching of fertilized eggs, and fry cultivation. Using the artificial breeding method of this invention, the success rate of spawning induction in female *Schizothorax lancensis* is 83.3%, the average fertilization rate is 87.5%, the average hatching rate is 93.0%, and the average fry emergence rate is 91.5%. This achieves large-scale artificial breeding of *Schizothorax lancensis*, laying the foundation for preventing the extinction of *Schizothorax lancensis*, and for carrying out the propagation and release of *Schizothorax lancensis* for industrial utilization.

The present invention discloses a reaction chamber system for atomic layer deposition of high conformal three-dimensional patterns, which includes a reaction chamber cavity and a reaction chamber cover. An equalizing chamber for delivering more than two precursor gases without mutual communication before the ejection of each precursor gas is provided in the reaction chamber cover. At least two groups of spray holes separately communicating with different equalizing chambers are provided on the bottom surface of the reaction chamber cover. Different spray holes communicating with different equalizing chambers are arranged alternately on the bottom surface of the reaction chamber cover and are arranged in a circular pattern. A rotatable workpiece table is provided in the reaction chamber cavity and is arranged parallel to the bottom surface of the reaction chamber cover during use. The reaction chamber system for atomic layer deposition of high conformal three-dimensional patterns of the present invention has the advantages of high utilization rate of precursor gases, convenient operation, low cost, good spray uniformity, easy control of the deposition process, high deposition efficiency, good film deposition uniformity, etc., can prepare high conformal three-dimensional patterns, has strong suitability, is convenient for industrial utilization, has high use value, and good application prospects.

The application discloses an amorphous metal oxide-carbon composite material and application thereof in electrocatalytic oxygen reduction synthesis of H2O2, wherein the amorphous metal oxide-carbon composite material comprises porous carbon PC and amorphous metal oxide MO loaded on the porous carbon x The amorphous metal oxide-carbon composite material has excellent catalytic activity, double-electron ORR selectivity and long-time durability. Moreover, the preparation method of the amorphous metal oxide-carbon composite material has a simple technological process, the amorphous characteristic of the obtained metal oxide is extremely innovative, raw material cost is low, and the amorphous metal oxide-carbon composite material is beneficial to regulation and control, and is favorable to popularization of large-scale industrial utilization of electrocatalytic oxygen reduction preparation of H2O2.

The present invention discloses a molybdenum phosphide composite catalyst and a preparation method thereof. The catalyst comprises nitrogen and phosphorus co-doped carbon nanosheets, on which molybdenum phosphide nanoparticles are loaded. The preparation method comprises the following steps: preparing a molybdenum-based metal organic framework / melamine dispersion, then adding a phytic acid solution for solvent thermal reaction and calcination. The molybdenum phosphide composite catalyst of the present invention has the advantages of strong catalytic activity and good stability, can be widely used for removing sulfur-containing organic compounds in petroleum products, can achieve efficient removal of sulfur-containing organic compounds in petroleum products under mild conditions, has high use value and good application prospects. The preparation method of the molybdenum phosphide composite catalyst of the present invention has the advantages of simple process, convenient operation, cheap and easily available raw materials, low cost, etc., can realize large-scale batch preparation, and is conducive to industrial utilization.

The invention discloses a cobalt nanoparticle / graphite-alkyne nano-composite catalytic material and a preparation method and application thereof.The preparation method comprises the following steps that graphite-alkyne is obtained and mixed with cobalt salt, nitrogen, nitrogen-dimethylformamide and absolute ethyl alcohol to prepare a mixed solution, the mixed solution is subjected to solvothermal reaction and calcination, and the cobalt nanoparticle / graphite-alkyne nano-composite catalytic material is obtained. The nanometer composite catalytic material is obtained. The prepared nano composite catalytic material has the advantages of being large in specific surface area, large in number of active sites, high in cobalt nanoparticle loading capacity, good in cobalt nanoparticle dispersity, high in catalytic activity, good in stability and the like, is a novel cobalt catalyst with excellent catalytic performance and stable structure, can efficiently activate persulfate when being used for activating the persulfate, and has good application prospects. Organic pollutants can be efficiently degraded, the use value is high, and the application prospect is good. The preparation method also has the advantages of simple process, convenience in operation, mild reaction conditions, low cost, environment friendliness and the like, can be used for large-scale preparation, and is convenient for industrial utilization.

The present invention relates to novel rebaudioside D and rebaudioside M-like steviol glycosides and a method for producing same. The novel rebaudioside D and rebaudioside M-like steviol glycosides obtained by the production method according to the present invention exhibit improved water solubility, and thus can be easily applied to beverages and foods with a high water content, and can significantly improve the industrial utilization of steviol glycosides.

The embodiment of the application relates to a low-density molded packaging product plant fiber material taking coconut fiber as raw material and a preparation method and application thereof, and belongs to the technical field of fiber packaging materials. The preparation method of the low-density molded packaging product plant fiber material taking coconut fiber as raw material comprises the following steps: step one, pretreatment; step two, chemical immersion; step three, grinding separation; step four, high-concentration immersion waste liquid extraction and impurity removal; and step five, fine grinding treatment. The fiber material provided by the embodiment of the application can improve the performance of molded products, further expand the application range of molded products, expand the industrial utilization range of characteristic plant fiber raw materials, improve the industrial utilization added value, reduce the dependence of the molded industry on paper pulp raw materials for papermaking, and relieve the pressure of insufficient paper pulp supply.

The utility model provides an RTO (Regenerative Thermal Oxidation) system suitable for high-concentration waste gas treatment, which comprises a regenerative oxidation furnace, a buffer tank and a detection unit, the gas outlet end of the buffer tank is connected with a combustion chamber in the heat accumulating type oxidation furnace after sequentially passing through a double-stop valve, a nitrogen purging device, a one-way valve and a flame arrester; the detection unit is arranged on the high-concentration waste gas inlet pipeline, and the detection unit and the double stop valves are in interlocking control. According to the RTO system, the oxygen content and the waste gas concentration in high-concentration waste gas can be monitored in real time through the detection unit, it is ensured that the oxygen content in the waste gas is lower than 2%, and the VOC concentration is lower than 25% or 20% of the minimum explosion lower limit, so that safety accidents such as explosion are effectively prevented, the whole RTO system can automatically adjust the operation mode according to the oxygen content, and the operation efficiency is improved. Manual intervention is reduced, the automation degree and operation stability of the system are improved, energy conservation, environmental protection and efficient operation are achieved, and the industrial utilization value is high.

This invention discloses an optimized method for a highly efficient fragmentation process of desert algae with high retention of active ingredients. The invention uses cell disruption rate, chlorophyll retention rate, and polysaccharide retention rate as core evaluation indicators, focusing on key parameters of the high-pressure homogenization fragmentation process (fracture pressure, fragmentation time, and algal liquor mass fraction). Effective ranges for these parameters are screened through single-factor experiments, and a multivariate synergistic regression model is constructed using response surface methodology to accurately predict the fragmentation effect and the retention rate of active ingredients. Experimental verification shows that the model has excellent fit; under optimal process conditions, the desert algae fragmentation rate is ≥91.5%, the chlorophyll retention rate is ≥76.9%, and the polysaccharide retention rate is ≥72.4%, significantly superior to existing ultrasonic fragmentation and enzymatic hydrolysis processes. This invention has advantages such as simple operation, high fragmentation efficiency, minimal loss of chlorophyll and polysaccharides, and high accuracy in predicting results. The fragmented products can be directly used in the development of desert algae fertilizers and other products, providing key technical support for the efficient industrial utilization of desert algae resources.

The present application relates to a kind of subsurface multi-parameter nanometer standard sample plate and its preparation method, including X tracking mark for locating and looking for calibration position, Y tracking mark and alignment mark, Z step calibration area for calibration, one-dimensional grid calibration area and two-dimensional grid calibration area, when using a kind of subsurface multi-parameter nanometer standard sample plate of the present application to carry out instrument calibration, current position can be quickly distinguished and positioned to the region to be measured by tracking mark and alignment mark, it can be used for the calibration and traceability of subsurface measuring instrument such as confocal microscope, white light interference microscope, ultrasonic atomic force microscope, provide guarantee for the accuracy of value in subsurface geometric parameter measurement, help the development of strategic emerging industries such as semiconductor, precision manufacturing, national defense and military industry, with high industrial utilization value.

The invention discloses a biocontrol bacillus amyloliquefaciens WYP1-4 for degrading feathers and application thereof, and belongs to the technical field of microorganisms. The bacterial strain is classified and named as bacillus amyloliquefaciens WYP1-4, and is preserved in the Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.65114, and the preservation address is the 5th floor, No.59 floor, Institute of Microbiology, Academy of Sciences, Guangdong Province. The feather degradation rate of the strain reaches 93% (28 h), and the strain has high industrial utilization value and has a good antagonistic effect on tea tree pathogenic bacteria pestalotiopsis pseudomonas CLBB1 and aspergillus niger YC01.

The present invention discloses a temperature-coupled sliding film differential resonant pressure sensor and a preparation method thereof. The sensor includes a pressure-conducting glass layer, a pressure-sensitive film layer and a resonant structure layer connected in sequence from bottom to top, and an aluminum film is arranged on the surface of the detection electrode of the resonant structure layer to form a temperature-sensitive composite detection electrode, which forms a double detection electrode with another detection electrode. The sensor of the present invention can use the detection signals of the double detection electrodes for differential calculation to eliminate the coupling effect of temperature on the sensor, thereby greatly improving the measurement accuracy of the sensor and realizing high-precision, temperature-coupled pressure measurement. It has the advantages of high measurement accuracy, strong anti-interference ability, and easy packaging. It is a new type of pressure sensor with excellent performance, which can be adapted to pressure measurement under different temperature conditions, has high use value, and has good application prospects. The preparation method of the present invention has the advantages of simple preparation, reliable process, low energy consumption, low cost, etc. It is suitable for large-scale preparation and convenient for industrial use.

The invention relates to the field of food microorganisms, and particularly discloses a bacillus pumilus T1-11 strain and an application of the bacillus pumilus T1-11 strain. The bacillus pumilus T1-11 is preserved in the China General Microbiological Culture Collection Center on January 10, 2025, and the preservation number is CGMCC NO.33025. The bacillus pumilus T1-11 has the characteristic of producing glutaminase, and can metabolize glutamine to generate glutamic acid; in addition, the strain T1-11 can also resist a salt environment, and the generated glutaminase can keep enzyme activity under the conditions of higher temperature and higher salinity. The bacillus pumilus T1-11 is further applied to soy sauce fermentation, the content of glutamic acid, isovaleraldehyde and amino nitrogen in soy sauce can be increased, and the delicate flavor of fermented food such as soy sauce can be improved. The bacillus pumilus T1-11 disclosed by the invention has an important industrial utilization value and a wide application prospect in the aspects of producing glutaminase and improving the delicate flavor of fermented foods.

The invention relates to a waste power battery black powder in-situ pyrolysis PVDF (Polyvinylidene Fluoride) fluorine fixation method, which comprises the following steps: performing pyrolysis treatment on waste power battery black powder in a mixed atmosphere of ammonia gas and oxygen; gas generated in the pyrolysis treatment process is absorbed, and fluorine fixation is achieved; the temperature of the pyrolysis treatment is 260 DEG C to 350 DEG C. According to the method provided by the invention, the mixed gas of ammonia gas and oxygen is used, so that the pyrolysis treatment temperature can be effectively reduced to 260-350 DEG C, and the energy consumption is reduced; the ammonia gas can be combined with HF generated by pyrolysis treatment to generate NH4F, so that the purpose of fixing fluorine is achieved; the produced NH4F has high industrial utilization value, and the economical efficiency of fluorine fixation is improved.

The application provides a ship CO2 hydrate capture system and method using waste heat energy and osmotic energy. The system comprises: a tail gas pretreatment module for sequentially performing cooling treatment, oxidation dust removal treatment and desulfurization and denitrification treatment on ship tail gas; a waste heat energy-osmotic energy power generation module for storing electric energy generated by organic Rankine cycle waste heat power generation and osmotic power generation; a hydrate method carbon capture module for bubbling and strengthening CO2 hydrate generation of flue gas after the tail gas pretreatment module, and forming hydrate slurry after CO2 hydrate accumulation, wherein a part of the hydrate slurry is subjected to CO2 capture, heating and pressure relief decomposition to produce purified water, and pure CO2 gas and purified water are obtained; another part of the hydrate slurry is subjected to filtration to obtain CO2 hydrate solid and storage; a CO2 transfer, utilization and storage module is used for directly storing the pure CO2 gas and the CO2 hydrate solid into the ocean or geology, or transporting the pure CO2 gas and the CO2 hydrate solid to downstream users for industrial utilization or biological utilization.

This invention provides a method for low-temperature water extraction of gallnut leaves, comprising the following steps: first, crushing the raw material to obtain gallnut leaf powder; mixing the gallnut leaf powder with pre-cooled purified water to form a liquid; adjusting the pH of the liquid at low temperature and then extracting; separating the solid and liquid at a low temperature of 5-18°C to remove waste residue and obtain a refined extract; further concentrating and purifying the refined extract to obtain a tannin extract. This method for low-temperature water extraction of gallnut leaves improves the comprehensive utilization rate of raw materials, simplifies the production process, reduces production costs, and improves production efficiency. It is suitable for the high tannin content of leaves from *Populus tomentosa* and *Populus thunbergii*, enabling the efficient and green industrial utilization of these raw materials.

The invention discloses a coal mine abandoned roadway series-parallel connection combined carbon dioxide energy storage system and an implementation method thereof.The implementation method comprises the steps that a to-be-used abandoned roadway is selected out and transformed into a high-position storage cavern, a middle-position storage cavern and a low-position storage cavern, and gaseous CO2, gas-liquid mixed CO2 and supercritical liquid CO2 are stored in the high-position storage cavern, the middle-position storage cavern and the low-position storage cavern respectively; excavating an equipment chamber to install an energy storage device, and connecting the high-position storage cavern, the middle-position storage cavern, the low-position storage cavern and the energy storage device through pipelines; under the driving of external electric quantity, gaseous CO2 is pressed into the middle-position storage cavern from the high-position storage cavern, and gas-liquid mixed CO2 is pressed into the low-position storage cavern from the middle-position storage cavern, so that energy storage is realized; the supercritical liquid CO2 in the low-position storage cavern expands to push the turbine to generate power and then enters the middle-position storage cavern, and the gas-liquid mixed CO2 in the middle-position storage cavern expands to push the turbine to generate power and then enters the high-position storage cavern, so that power generation is realized. According to the invention, CO2 industrial utilization and mine underground space development can be synergistically realized.

The invention discloses an Apertus pallidofulvus Z-12 strain which is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 2, 2025, and the preservation number of the Apertus pallidofulvus Z-12 strain is GDMCC No: 66621. The Apertus pallidofulvus Z-12 strain is named as Apertus pallidofulvus Z-12 strain. According to the invention, several strains are separated from naturally stored tea seed meal, fresh tea seed meal is inoculated for fermentation, and in combination with an alpha-glucosidase inhibition rate, a new strain Aperbacillus pallidofulvus Z-12 for high yield of hypoglycemic active matters from fermented tea seed meal is screened and identified. Meanwhile, the Z-12 strain and the protease are innovatively and synergistically fermented under the optimal process condition, and the quality of the blood sugar reducing active matter in the tea seed meal is remarkably improved. The technology not only fills the technical blank of efficient utilization of high-quality protein of the tea seed meal, but also provides a brand new thought for industrial utilization of the tea seed meal.