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Method for extracting fucoxanthin from brown algae

A fucoxanthin and brown algae technology, applied in the direction of organic chemistry, etc., can solve problems such as affecting the purification of fucoidin and the inability of samples to be dried.

Active Publication Date: 2010-12-15
秦皇岛惠恩生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] (1) Dimethyl sulfoxide has good miscibility with various solvents, and can be dissolved in most organic solvents such as ethanol, propanol, benzene and chloroform. After extraction with ethyl acetate and ammonium sulfate solution, There are residues, and dimethyl sulfoxide has a high boiling point, which cannot be removed by low-temperature vacuum distillation, so that the sample cannot be dried, and its existence will affect the subsequent purification of fucoxanthin;

Method used

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  • Method for extracting fucoxanthin from brown algae
  • Method for extracting fucoxanthin from brown algae
  • Method for extracting fucoxanthin from brown algae

Examples

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Embodiment 1

[0071] The light-dried kelp was washed twice with tap water at room temperature to remove surface silt, floating organisms, colloids and other sundries, then air-dried naturally in the shade, and then crushed with a grinder produced by Jiangyin Xinyou Machinery Manufacturing Co., Ltd. 30 mesh or less, and then stored in a refrigerator at a temperature of 4°C until use.

[0072] Put 1g of crushed kelp in an iodine bottle and soak it in 4ml of water for 10 minutes until the kelp fully absorbs water and the surface of the kelp becomes dry. Then, it was leached with 5ml of acetone water-soluble organic solvent for 30min at room temperature and protected from light, and the leaching was repeated twice. At the same time, under the same conditions, the same kelp without swelling treatment was leached with acetone or 75% by volume acetone aqueous solution for 30 min, and the leaching was repeated twice. The extracts were collected respectively and centrifuged to remove insoluble matt...

Embodiment 2

[0081] This embodiment is carried out in the same manner as in Example 1, except that 5 ml of acetone, dimethyl sulfoxide, and methanol water-soluble solvents are used for leaching once for 30 min. The results of these assays are listed in Table 2.

[0082] Table 2: Absorbance values ​​of fucoxanthin, chlorophyll c and chlorophyll a when extracted with acetone, DMSO and methanol (diluted 3 times)

[0083]

[0084] It can be clearly seen from Table 2 that the efficiency of extracting fucoxanthin with acetone after the method of the present invention is much higher than that of using other solvents (DMSO, methanol) under the same conditions.

Embodiment 3

[0086] The present embodiment is carried out in the same manner as in Example 1, except that the pulverized sea-tangle of 1 g is extracted once with 4, 5, 6, and 7 ml of acetone for 30 minutes. The results of these assays are listed in Table 3.

[0087] Table 3: Absorbance values ​​of fucoxanthin, chlorophyll c and chlorophyll a when extracted with different amounts of acetone (diluted 3 times)

[0088]

[0089] It can be clearly seen from Table 3 that under the method conditions of the present invention, when the ratio of the weight of kelp in grams to the volume of acetone in milliliters is 1:5, the efficiency of extracting fucoxanthin is better than other ratios.

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Abstract

The invention relates to a method for extracting fucoxanthin from brown algae. The method comprises the following steps of: performing water swelling pretreatment on the crushed brown algae, and leaching the brown algae by using a water-soluble organic solvent; purifying fucoxanthin in leaching solution by adopting an extraction method and twice column chromatography in turn; and finally, further purifying to obtain a fucoxanthin standard substance with the purity of over 90 percent or over 99 percent through preparative thin-layer chromatography or preparative high performance liquid chromatography. The method for extracting the fucoxanthin has the advantages of simple, economic and efficient technology, and capacity of providing an experimental basis for industrial production of the fucoxanthin and improvement of industrial utilization rate of the algae.

Description

【Technical field】 [0001] The invention belongs to the technical field of natural product extraction. More specifically, the present invention relates to a method for extracting fucoxanthin from brown algae. 【Background technique】 [0002] Fucoxanthin, also known as fucoxanthin and fucoxanthin, is an important carotenoid, which is yellowish brown in color and has a very strong antioxidant effect. The molecular formula is C 42 h 58 o 6 , CAS accession number 3351-86-8. [0003] Fucoxanthin exists in brown algae and diatoms in large quantities, and has not been found to exist in other algae and terrestrial plants. As a strong antioxidant, fucoxanthin is even better than vitamin C and vitamin E. It has the functions of anti-aging, enhancing immunity, preventing and treating cancer, and can be added to over-the-counter medicines or nutritional health products. The research team led by Professor Masashi Hosokawa of the Graduate School of Hokkaido University in Japan found tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D303/32C07D301/32
Inventor 吕广孟永宏徐志文
Owner 秦皇岛惠恩生物技术有限公司
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