Method for producing saponin and organic acid by using turmeric

An organic acid and turmeric technology, applied in the field of bioengineering, can solve the problems of large discharge of waste water and waste residue, inability to effectively utilize turmeric starch, etc., and achieve the effects of clean process, large industrial application value, and high extraction rate.

Inactive Publication Date: 2010-08-25
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method for producing saponin and organic acid by using turmeric, which solves the problems of large amount of waste water and waste residue extracted from turmeric raw materials and inability to effectively utilize turmeric starch

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 comprises the following steps:

[0037] 1. The step of releasing turmeric saponins;

[0038] After 300 grams of dried turmeric roots are pulverized, add 900 grams of water to obtain a mixture of turmeric and water; then the mixture of turmeric and water is processed as follows:

[0039] Under the conditions of temperature 120°C and pressure 0.1MPa, steam exploded for 15 minutes and cooled to room temperature; add 2400 grams of water and mix well, add pectinase 2.4×10 4 .U, Cellulase 4.8×10 4 ; Mix evenly, and stir for 20 hours at a pH value of 4.0 and a temperature of 45° C. to obtain a turmeric starch solution;

[0040] 2. The saccharification step of turmeric starch;

[0041] Add 2400U of α-amylase to the turmeric starch solution, stir for 20min at pH 5.0 and temperature 50°C, and cool to room temperature; then add 2.4×10 glucoamylase 4 .U, stirring at a temperature of 50°C for 20 hours to obtain turmeric saccharification liquid;

[0042] Then the tu...

Embodiment 2

[0057] Embodiment 2 comprises the following steps:

[0058] 1. The step of releasing turmeric saponins;

[0059] After 1 kilogram of dry turmeric roots are pulverized, 5 kilograms of water are added to obtain a mixture of turmeric and water; then the mixture of turmeric and water is processed as follows successively:

[0060] Under the conditions of temperature 130°C and pressure 0.2MPa, steam exploded for 20 minutes, cooled to room temperature; add 24 kg of water to mix, add pectinase 1.2×10 5 .U, Cellulase 2.5×10 5 U; Stirring for 30 hours at a pH value of 5.0 and a temperature of 55° C. to obtain a turmeric starch solution;

[0061] 2. The saccharification step of turmeric starch;

[0062] Add α-amylase 1.5 × 10 to the turmeric starch solution 4 U, stirred at pH 7.0 and 95°C for 40 min, cooled to room temperature; then added 1.5×10 glucoamylase 5 U, stirring at a temperature of 65° C. for 30 h to obtain turmeric saccharification liquid;

[0063] Then heat the turmeric...

Embodiment 3

[0078] Embodiment 3 comprises the following steps:

[0079] 1. The step of releasing turmeric saponins;

[0080] After 5 kilograms of dried turmeric roots are pulverized, 20 kilograms of water are added to obtain a mixture of turmeric and water; then the mixture of turmeric and water is processed as follows successively:

[0081] Under the conditions of temperature 125°C and pressure 0.15MPa, steam exploded for 17min and cooled to room temperature; add 75kg of water and mix well, add pectinase 5×10 5 U, Cellulase 1×10 6 U; stirring for 25 hours at a pH value of 4.5 and a temperature of 50° C. to obtain a turmeric starch solution;

[0082] 2. The saccharification step of turmeric starch;

[0083] In the turmeric starch solution, add α-amylase 5 * 10 4 U, stirred at pH 6.0 and temperature 70°C for 30 min, cooled to room temperature; then added 5×10 glucoamylase 5 U, stirring at a temperature of 60° C. for 25 hours to obtain turmeric saccharification liquid;

[0084] Then t...

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Abstract

The invention discloses a method for producing saponin and organic acid by using turmeric, which belongs to the field of biological engineering and solves the problem that the conventional method for extracting turmeric saponin by using a turmeric raw material has large discharge amounts of waste water and waste slag and cannot effectively use turmeric starch. The method comprises the steps of turmeric saponin release, turmeric starch saccharification, fermentation medium preparation, organic acid fermentation, saponin enrichment and saponin preparation. By pretreating the turmeric raw material by using fermentation and membrane separation coupling, the method has the advantages of pollution-free production of the saponin and the organic acid, high turmeric saponin extracting rate, full utilization of the turmeric starch, little waste water amount and little production during turmeric saponin production, pollution-free process and great industrial utilization value in the turmeric industry.

Description

technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a method for producing saponin and organic acid from turmeric, which uses fermentation and membrane separation coupling to pretreat turmeric raw material to cleanly produce saponin and organic acid. Background technique [0002] Yellow ginger, formal name used at school as Dioscorea shield leaf, perennial herb, is a unique medicinal material in my country, containing 2 to 3% saponin (diosgenin); saponin is the primary raw material of steroid hormone drugs, which can be processed into more than 180 kinds of drugs, Known as the gold in medicine. Turmeric also contains 30-40% starch and 40-50% fiber, so it has a wide range of uses. The traditional process of using turmeric to extract saponin is to hydrolyze turmeric starch together with cellulose, pectin, etc. with acid, which not only makes it difficult to reuse the starch, but also uses a large amount of acid, resulting in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/20C12P7/56C12P7/46C07J71/00C07C51/42C07C55/10C07C59/08B01D61/14
Inventor 余龙江卢正东鲁明波何峰魏蜜何谧
Owner HUAZHONG UNIV OF SCI & TECH
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