40 results about "Cytochalasin" patented technology

The invention relates to a preparation method and a purpose of a cytochalasin compound. According to the invention, a novel cytochalasin compound is produced by using Aspergillus flavipes ZHN1-09 in a Guangxi mangrove forest plant Cerbera manghas root mug sample. It is proved by experiments that the compound can be used as cell proliferation inhibitors or antitumor agents.

The invention provides a temperature-sensitive vascular thrombosis material capable of performing long-term self-development and a preparation method thereof. The vascular thrombosis material contains gold nano gel and a dispersion medium, wherein the gold nano gel comprises a gold nano particle cytochalasin and a polyacrylamide amide compound temperature-sensitive polymer housing layer coated on the surface of the gold nano particle cytochalasin. The vascular thrombosis material only uses temperature as a phase change regulating and controlling factor, has good biocompatibility, can achieve long-term thrombosis in the blood vessel, has the capability of long-term development, can be used for vascular thrombosis of multiple tumor sites, achieves thrombosis of the aorta and terminal blood vessel, completely blocks blood and cuts off cancer cell blood supply to achieve the tumor suppression purpose.

The invention discloses a synthesis method of flavipesine A, a cytochalasin compound. The structural formula of the compound is: the synthesis method comprises: 1) dissolving compound B in tetrahydrofuran, adding a reducing agent at room temperature, and reacting at 40°C to obtain Intermediate C; 2) At room temperature, add the reaction reagent to the THF solution of Intermediate C to obtain Compound A. The reagents used in the synthesis process provided by the present invention can be purchased commercially at low prices, and this reaction strategy is also applicable to the synthesis of other types of cytochalasin-like intermediate product derivatives, so as to study the structure modification and structure-activity relationship of this type of alkaloids , New drug development and mass production lay the foundation.

The invention discloses a preparation and identification method of a human leukemia cell cytoplast. The method comprises the following steps of: treating a purified human leukemia HL-60 cell with a cytochalasin and colchicine, centrifugally denucleating at the temperature of 34 DEG C, collecting a cytoplast component, and purifying with a Percoll density gradient centrifugation method to obtain apurified cytoplast; and identifying the cytoplast with DAPI (4',6-diamidino-2-phenylindole) and CFSE (5,6-carboxyflu-orescein diacetate succinimidyl ester) fluorescent double staining. Due to the adoption of the method, the denucleating rate of a non-adherent human leukemia HL-60 cell can be over 90 percent, the purity of purified cytoplasts is over 95 percent, the quantity of cytoplasts which are more than or equal to 5 mum in diameter is up to 81 percent, and a cytoplast which is chromophilic with a CFSE fluorescent probe can be directly applied to subsequent researches; an HL-60 cell cytoplast obtained with the method can be widely applied to researches of leukemia cell cytoplast components as well as metabolism, functions, cell reconstruction, cell differentiation, apoptosis and the like thereof; and the method is suitable for preparing and identifying all in-vitro non-adherent tumor cell cytoplasts.