40 results about "Cytochalasin" patented technology

The present invention provides commercial utilization of a novel yeast strain Zygosaccharomyces rouxii and its fermented metabolites as probiotic, as an antioxidant and as an antimicrobial agent in foods and cosmetics. The fermented broth and metabolite substance (s) produced by this invention have a wide spectrum antibacterial activity, strong antioxidant activity, cytochalasin-like activity that inhibits cell cleavage, and is expected to be effective in the treatment of allergy, atopic dermatitis, psoriasis and various skin diseases, second degree burns, high blood pressure, diabetes, cancer, AIDS and as an anti-aging agent. Moreover, this invention is expected to lead to industrial utilization of Z.rouxii for manufacturing succinic acid and malic acid from a yeast.

The slow releasing injection containing antimiotic medicine belongs to the field of slow releasing anticancer medicine technology. The injection includes the components of anticancer medicine, slow releasing supplementary material, suspending agent and / or solvent. The anticancer medicine includes cytochalasin, colchicines, beta-naphthol, acodazole, giracodazole, nocodazole or other antimiotic medicine; the slow releasing supplementary material is polylactic acid, glycolic acid-hydroxyacetic acid copolymer, EVA, polifeprosan or their composition; the suspending agent is carboxymethyl cellulose, mannitol, etc.; the solvent is selected from distilled water, injection water, anhydrous alcohol, etc. The slow releasing anticancer injection may be injected in different modes and has reduced systemic toxic reaction, raised local medicine concentration and raised treating effect.

The invention relates to the technical field of cells, in particular to a method for preparing exosomes of mesenchymal stem cells. The method comprises the following steps that a culture solution containing gelatin microcarriers is prepared; mesenchymal stem cells are inoculated into the culture solution for stirring culture; and when the confluence of growth of cells to be attached to the gelatinmicrocarriers reaches 80%-90%, recombinant interferon gamma and cytorelaxin D are added for further culture for 12-48h, and a conditioned medium is collected to extract the exosomes. According to themethod, gelatin microspheres prepared by genipin are used for the cultivation of mesenchymal stem cells, the recombinant interferon gamma and the cytochalasin D are added in the specific period of culture, compared with the prior art, the secretion of the exosomes is significantly improved, more exosomes with biological activity are obtained, and the large-scale exosome acquisition and large-scale preparation can be realized.

Methods and compositions for the prevention and / or treatment of metastatic cancer by tumor encapsulation achieved by introducing a physical obstacle to prevent cancerous cells from escaping the original site and invading other tissues. Physical obstacle is introduced as a pre-formed matter or its assembly by the treated organism is induced. In the preferred embodiment the composition of matter is disclosed, which enables formation of the obstacle in a form of basement membrane (BM). Said composition enables tissue cells to assemble BM on their surfaces, and supplies ECM components for said assembly, whereby BM is assembled de novo or the existing BM is strengthens. The composition comprises soy phytoestrogens, including genistein, daidzein, glycitein, biochantin A, and derivative thereof, actin cytoskeleton reorganization inhibitors, such as Cytochalasin D, and PTK inhibitors, in combination with ECM proteins, such as laminins and type IV collagens, or their derivatives.

The invention provides an application of a cytochalasin category compounds in a herbicide and belongs to the technical field of pharmaceutical chemistry. According to the invention, the cytochalasin category compounds includes a compound with any one structure shown in the formula AL-11-18 or a physiologically acceptable salt thereof. The cytochalasin category compounds have phytotoxicity and canbe used as an effective component of a herbicide. Example results show that the cytochalasin category compounds have an inhibition effect on arabidopsis thaliana rooting.

The invention provides a temperature-sensitive vascular thrombosis material capable of performing long-term self-development and a preparation method thereof. The vascular thrombosis material contains gold nano gel and a dispersion medium, wherein the gold nano gel comprises a gold nano particle cytochalasin and a polyacrylamide amide compound temperature-sensitive polymer housing layer coated on the surface of the gold nano particle cytochalasin. The vascular thrombosis material only uses temperature as a phase change regulating and controlling factor, has good biocompatibility, can achieve long-term thrombosis in the blood vessel, has the capability of long-term development, can be used for vascular thrombosis of multiple tumor sites, achieves thrombosis of the aorta and terminal blood vessel, completely blocks blood and cuts off cancer cell blood supply to achieve the tumor suppression purpose.

The invention relates to a tetraploid chemical induction method suitable for clam philippines. The main content of the method includes: fully promoting the ripening of clams of philippines; Fertilized eggs with high concentration were obtained by artificial insemination; 13 minutes after fertilization, cytochalasin CB was added to treat at a concentration of 0.5 mg / L to inhibit the release of the first polar body; 19 minutes after fertilization, the eggs were washed to remove CB; The fertilized eggs were developed under suitable conditions, and tetraploid Philippine clam embryos were obtained; the seawater used in the processing and development process was sand-filtered seawater, with a salinity of 24-26 and a temperature of 24.8-25°C. The invention utilizes the best sperm and eggs, effectively improves the developmental synchronization of fertilized eggs, significantly improves the tetraploid induction rate, and the induction rate detected by flow cytometry is stable at over 50%.

The invention relates to a preparation method and a purpose of a cytochalasin compound. According to the invention, a novel cytochalasin compound is produced by using Aspergillus flavipes ZHN1-09 in a Guangxi mangrove forest plant Cerbera manghas root mug sample. It is proved by experiments that the compound can be used as cell proliferation inhibitors or antitumor agents.

The invention discloses a preparation and identification method of a human leukemia cell cytoplast. The method comprises the following steps of: treating a purified human leukemia HL-60 cell with a cytochalasin and colchicine, centrifugally denucleating at the temperature of 34 DEG C, collecting a cytoplast component, and purifying with a Percoll density gradient centrifugation method to obtain apurified cytoplast; and identifying the cytoplast with DAPI (4',6-diamidino-2-phenylindole) and CFSE (5,6-carboxyflu-orescein diacetate succinimidyl ester) fluorescent double staining. Due to the adoption of the method, the denucleating rate of a non-adherent human leukemia HL-60 cell can be over 90 percent, the purity of purified cytoplasts is over 95 percent, the quantity of cytoplasts which are more than or equal to 5 mum in diameter is up to 81 percent, and a cytoplast which is chromophilic with a CFSE fluorescent probe can be directly applied to subsequent researches; an HL-60 cell cytoplast obtained with the method can be widely applied to researches of leukemia cell cytoplast components as well as metabolism, functions, cell reconstruction, cell differentiation, apoptosis and the like thereof; and the method is suitable for preparing and identifying all in-vitro non-adherent tumor cell cytoplasts.

The invention provides an induction method of a high-heterozygosity tetraploid of a new variety of crassostrea gigas 'Haida No.3'. The method comprises the following steps: taking female triploid crassostrea gigas 'Haida No.3' obtained by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas 'Haida No.3' as a female parent; taking a male diploid 'Haida No.3' as a male parent; after the female parent and the male parent are artificially matured, putting ova into seawater to be matured, and when the ova are round through microscopic examination, the ova are matured; conducting insemination on the cured ova and sperms, starting timing after the sperms and the ova are mixed, treating fertilized ova with cytochalasin B, inhibiting discharging of the first polar bodies of the fertilized ova, and collecting and soaking the fertilized ova; and transferring the fertilized ova into a cultivation container for incubation and larva cultivation. The triploid female parent shellfish is obtained by inhibiting the discharge of the first polar bodies of the fertilized ova of the diploid crassostrea gigas 'Haida No.3', and the tetraploid obtained by the triploid female parent shellfish has higher growth advantage due to higher gene heterozygosity.

The invention discloses a preparation method and application of a cytochalasin compound Aspochalasin D, and belongs to the technical field of medicines. The preparation method comprises the following steps: inoculating aspergillus flavipes Z-4 of which the preservation number is CCTCC NO: M 2013631 into a rice culture medium, performing fermentation culture, and separating the cytochalasin compound Aspochalasin D from a fermentation liquid. According to the preparation method, the marine fungus, namely the aspergillus flavipes Z-4 is adopted for fermentation culture in the rice culture medium for a first time, the Aspochalasin D is prepared through extraction and separation, and a novel preparation mode is provided for production of the compound; in addition, the preparation method is simple, convenient and easy to operate, low in production cost and applicable to on-scale production; and the Aspochalasin D obtained from a fermentation product of the aspergillus flavipes Z-4 has a remarkable function of inhibiting activity of prostatic cancer cell proliferation, so that the compound can be developed into prostatic cancer prevention medicines or prostatic cancer prevention healthcare food.

According to the present invention, a Phomopsis asparagi DHS-48 fermentation product is further separated and purified to obtain a compound having a novel structure, and the evaluation on the cytotoxicity and the immunosuppressive activity of the compound proves that the compound has low cytotoxicity and significant immunosuppressive activity on mouse splenocytes. The study on the mechanism of action shows that the compound is a potential immunosuppressor capable of inhibiting the dephosphorylation of the intracellular phosphorylated NFAT protein, and can be used for preparing immunosuppressive drugs aiming at organ transplantation and autoimmune diseases.

Embodiments of the invention include compositions and methods for the inhibition of pathogen-mediated cytopathic effects by contacting a cell with an 1-CaD polynucleotide or polypeptide. In still further embodiments, a nucleic acid encoding 1-CaD is administered to cell infected by or at risk of infection by a pathogen. Gene delivery of 1-CaD shows a reduced cell toxicity compared to cytochalasin. The delivery of 1-CaD affords a protection on or therapy for modulating cell membrane integrity for protection against an infection.

The invention relates to a cell membrane microvesicle targeting an inflammation region and application of the cell membrane microvesicle, and solves the technical problem that an existing material does not have the characteristic of efficiently targeting the inflammation region. A preparation method of the cell membrane microvesicle comprises the following steps: transfecting an MC-3T3 cell through CXCR4 gene overexpressing lentivirus by utilizing genetic engineering, and proliferating the transfected cell to obtain cells with overpressed membrane receptor CXCR4; culturing the over-expressed CXCR4 MC-3T3 cells, conducting washing, conducting treating with cytochalasin B, and carrying out vortex separation on the cells and cell membrane microvesicles; centrifuging the mixture, separating the cells from the cell membrane microvesicles, collecting supernate, and conducting centrifuging for the second time to obtain the cell membrane microvesicles of the targeted inflammation region. The invention also provides an application of the cell membrane microvesicle of the targeted inflammation region in preparation of a targeted inflammation region material. The method can be used in the field of preparation of drug loading materials.

The invention discloses a reagent kit and method for dissociating an animal embryo. The reagent kit for dissociating an animal embryo disclosed by the invention comprises reagents of which the names are respectively an enzymolysis solution C, an enzymolysis solution D and an enzymolysis solution E, wherein the enzymolysis solution C consists of cytochalasin storing fluid and TrypLE digestive fluid, and the volume ratio of the cytochalasin storing fluid to the TrypLE digestive fluid is (3 to 47) to (3 to:97); the enzymolysis solution D is fluid consisting of fetal calf serums, a pronase solution and a TCM-HEPES buffer solution, and the volume ratio of the fetal calf serums to the pronase solution to the TCM-HEPES buffer solution is (1 to 1 to 1) to (1 to 2 to 1); and the enzymolysis solution E consists of cytochalasin storing fluid and Accutase cell separation fluid, and the volume ratio of the cytochalasin storing fluid to the Accutase cell separation fluid is (3 to 47) to (3 to 97). Experiment proves that when the reagent kit and method disclosed by the invention are used for dissociating the animal embryo, the dissociating efficiency is high, the embryo of an animal of species, which cannot be dissociated by an existing method, can be dissociated, the reagent kit and the method are suitable for the embryos at different developmental stages, and hurt to cells in the dissociating process can also be reduced.

The invention discloses a culture medium capable of improving osteogenic differentiation efficiency of human mesenchymal stem cells and a culture method. The culture medium is prepared by periodically adding cytochalasin D into a traditional human stem cell osteogenic differentiation culture medium. The culture method comprises an early-stage culture medium and a later-stage culture medium, wherein the early-stage culture medium contains cytochalasin D. The early-stage culture medium is used for the first 14 days of human mesenchymal stem cell in-vitro osteogenesis induction, and the later-stage culture medium is used for 14-28 days of in-vitro osteogenesis induction. By adopting the osteogenic induction culture solution, the alizarin red dyeing effect can be remarkably improved, meanwhile, the expression of osteogenic related genes and proteins COL1A1 and OCN in the later period of osteogenic differentiation of the human mesenchymal stem cells is remarkably promoted, and the results show that the osteogenic differentiation efficiency of the human mesenchymal stem cells can be remarkably improved, and the result indicates that the method can be used for bone tissue regeneration and repair in stem cell treatment.

The invention relates to a novel cytochalasin compound with a function of antagonizing clinical drug-resistant bacteria and a preparation method of the novel cytochalasin compound, and belongs to the technical field of biomedicine. The method comprises the following steps: firstly, carrying out fermentation culture on Cytospora chrysosperma; then extracting a secondary metabolite obtained by fermenting and culturing the Cytospora chrysosperma by using methanol, conducting extracting by using ethyl acetate, conducting concentrating under reduced pressure, carrying out gradient elution on the obtained crude extract to obtain six component segments, and carrying out gradient elution on the fourth component segment again to obtain four sub-component segments; and respectively carrying out sephadex chromatography separation and purification and high performance liquid chromatography separation and purification on the second subgroup segment and the fourth subgroup segment to obtain two novel cytochalasins. The two novel cytochalains have a very strong antagonistic effect on four clinical drug-resistant bacteria, namely carbapenem-resistant pseudomonas aeruginosa, methicillin-resistant staphylococcus aureus, multi-drug-resistant enterococcus faecalis and multi-drug-resistant enterococcus faecium, and are expected to be developed into novel drugs for resisting the clinical drug-resistant bacteria.

The present invention relates to a method of inducing fenestrae in an endothelial cell line. More particularly, the present invention relates to inducing fenestrae in a bEND5 cell line or a Py4.1 cell line utilizing latrunculin A or cytochalasin B as an inducing agent.

The invention relates to a microbial metabolite and an application technology thereof in the field of drug research, and concretely relates to a preparation method and application of a cytochalasin compound produced by microbial metabolism. The chemical structure of the cytochalasin compound is shown as a formula I, and the cytochalasin compound is prepared by carrying out fermentation culture on Curvularia verruculosa. Experiments show that the compound has anti-tumor activity and can be used as a cell proliferation inhibitor or an anti-tumor preparation. The prepared cytochalasin compound is produced by fermentation culture of microorganisms, and has the characteristics of simplicity in operation, short production period, low cost and the like.

The invention discloses a low-temperature preservation method of a multifunctional 3D recombinant skin model. According to the invention, cytochalasin B is applied to solid preservation of a skin model for the first time, a three-step method is adopted for treatment, and the treatment method can maintain the cell activity of the model in a transportation process and reduce cell hypoxic injury, sothat the test accuracy and the cell activity of the 3D heavy skin group model are guaranteed, and the preservation time is prolonged to a great extent.

The invention relates to an induction method for a 'Haida 2#' new variety tetraploid of crassostrea gigas. The induction method comprises the steps of selecting a 'Haida 2#' new variety of crassostreagigas as parent oysters, carrying out manual maturity-promoting culture until the parent oysters are mature, selecting female and male individuals with good gonad development as parent oysters, dissecting for fetching ova, filtering, putting the ova into filtered seawater for maturation, enabling the maturated ova to be fertilized with sperms in the filtered seawater, processing fertilized ova byvirtue of cytochalasin B so as to inhibit the discharging of first polar bodies, after the processing, collecting the fertilized ova, transferring the fertilized ova into an ethanol solution for soaking, finally transferring the fertilized ova into a culture container, and carrying out incubation and larva culture. According to the induction method, the discharging of the first polar bodies of the fertilized ova of the ''Haida 2#'' new variety of a diploid is inhibited by virtue of the cytochalasin B, and the living 'Haida 2#' new variety tetraploids of crassostrea gigas are directly induced,so that the survival rate is greatly increased, and a seedling culturing foundation is provided for the amplified breeding of the 'Haida 2#' new variety tetraploid of crassostrea gigas.

The invention discloses a low-temperature preservation method of a multifunctional 3D recombined skin model. The invention applies cytochalasin B to the solid preservation of the skin model for the first time, and adopts a three-step method for processing. The treatment method can maintain the model during transportation The cell activity in the skin can reduce the hypoxic damage of the cells, so as to ensure the accuracy of the 3D re-skin group model test and the vitality of the cells, and greatly extend the storage time.

The invention relates to a circulating tumor cell detection method and application thereof, and relates to the field of detection and capture of circulating tumor cells. The detection method comprises the following steps: treating circulating tumor cells by adopting cytochalasin D to damage filamentous actin of the circulating tumor cells. According to the detection method, by changing the mechanical property of the cell membrane of the circulating tumor cells, the deformation energy of the treated tumor cells plays a leading role in comparison with the adhesion energy, the tumor cells are promoted to be adhered to the surface of the substrate, and the detection efficiency of the tumor cells is further improved. Compared with the prior art, the detection method is simple and convenient, does not need complex steps, is low in cost, does not need expensive reagents and equipment, and the cytochalasin D used in the detection method does not cause damage to cell activity.

The present disclosure relates to an antiaging composition containing cytochalasin D or SAG, and a method for screening an antiaging substance, and provides a new antiaging substance and a new method for discovering a new antiaging substance. Thus, it is possible to greatly contribute to the development of beauty industries and to meeting demands related with beauty and antiaging, if the present disclosure is effectively used.

The invention discloses a culture solution for mechanical exfoliation of cytoplasm and a use method thereof, the culture solution comprises in vitro operation culture solution basic components, additive components and the like, and the culture solution is characterized in that the additive components comprise substances for softening cell membranes during mechanical exfoliation of cytoplasm; this includes, but is not limited to, the addition or combined addition of polyvinylpyrrolidone (PVP), cytochalasin, protein components (such as serum or serum proteins or substitutes thereof) at appropriate concentrations. The culture solution is favorable for realizing smooth separation of cytoplasm from cell bodies under the action of mechanical force under the conditions of micromanipulation and the like, that is, when the culture solution is used, ovum, cells or partial cell bodies taken down from the ovum are placed in the culture solution, so that cell membranes become soft and viscous, and when the mechanical force is applied, for example, a microscopic tube is used for sucking one part of the cell body to swing or extrude and the like, the cytoplasm of the stressed part is easily separated from the primitive cell body, the cell membrane of the separated part and the non-separated part is still kept complete, and the cell membrane disintegration is not easy to occur.

The invention discloses a preparation and identification method of a human leukemia cell cytoplast. The method comprises the following steps of: treating a purified human leukemia HL-60 cell with a cytochalasin and colchicine, centrifugally denucleating at the temperature of 34 DEG C, collecting a cytoplast component, and purifying with a Percoll density gradient centrifugation method to obtain apurified cytoplast; and identifying the cytoplast with DAPI (4',6-diamidino-2-phenylindole) and CFSE (5,6-carboxyflu-orescein diacetate succinimidyl ester) fluorescent double staining. Due to the adoption of the method, the denucleating rate of a non-adherent human leukemia HL-60 cell can be over 90 percent, the purity of purified cytoplasts is over 95 percent, the quantity of cytoplasts which are more than or equal to 5 mum in diameter is up to 81 percent, and a cytoplast which is chromophilic with a CFSE fluorescent probe can be directly applied to subsequent researches; an HL-60 cell cytoplast obtained with the method can be widely applied to researches of leukemia cell cytoplast components as well as metabolism, functions, cell reconstruction, cell differentiation, apoptosis and the like thereof; and the method is suitable for preparing and identifying all in-vitro non-adherent tumor cell cytoplasts.

The invention discloses an iodine-containing cytochalasin derivative derived from marine fungi as well as a preparation method and application of the iodine-containing cytochalasin derivative. According to the invention, a strain of marine mangrove forest endophytic fungus Phomopsis sp.QYM-13 is obtained by screening from healthy leaves of a mangrove plant Kandelia candel, and an iodine-containing cytochalasin derivative inphomopchalasin with anti-inflammatory activity is extracted from the marine mangrove forest endophytic fungus Phomopsis sp.QYM-13. The invention not only provides a new drug source for the preparation of anti-inflammatory drugs, but also has the advantages of simple preparation method and low cost, and is suitable for large-scale industrial application.

The invention provides an improved micromanipulation solution for a mitochondrial replacement technology and a preparation method of the improved micromanipulation solution. The improved micromanipulation liquid for the mitochondrial replacement technology is prepared from cytochalasin B, dimethyl sulfoxide and a vitrolite assisted reproduction IVF-gamete treatment liquid, in the improved micromanipulation solution, the concentration of cytochalasin B is greater than 0 mu g / mL and less than 2.5 mu g / mL. According to the improved micromanipulation liquid for the mitochondrial replacement technology, the purpose of improving the effectiveness and safety of the mitochondrial replacement technology is achieved only by adjusting the concentration of cytochalasin B, operation is easy, and safety guarantee is provided for thoroughly blocking maternal-fetal heredity of mitochondrial diseases.