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Marine fungus-derived iodine-containing cytochalasin derivative as well as preparation method and application thereof

A technology of cytochalasin and its derivatives, applied in the field of biomedicine, can solve problems such as liver damage and adverse reactions, and achieve the effect of low cost and simple preparation method

Pending Publication Date: 2022-08-09
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term large-scale use will produce a series of adverse reactions, side effects and tolerance, such as gastric mucosal damage, liver damage, kidney damage, etc.

Method used

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  • Marine fungus-derived iodine-containing cytochalasin derivative as well as preparation method and application thereof
  • Marine fungus-derived iodine-containing cytochalasin derivative as well as preparation method and application thereof
  • Marine fungus-derived iodine-containing cytochalasin derivative as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Isolation and identification of marine mangrove endophytic fungus Phomopsis sp.QYM-13

[0041] In the present invention, an endophytic fungus with anti-inflammatory activity is isolated from the healthy leaves of mangrove plant Kandelia candel collected in Dongzhaigang, Hainan, China. The endophyte was identified by ITS rRNA as Phomopsis sp. fungus, so it was named Phomopsis sp. QYM-13, and was deposited in the Guangdong Provincial Collection of Microorganisms on January 21, 2022 Center, the preservation address is Building 59, No. 100, Xianlie Middle Road, Guangdong City, and the preservation number is GDMCC NO: 62207.

Embodiment 2

[0042] Example 2 Isolation and identification of iodine-containing cytochalasin derivatives

[0043] In the present invention, an iodine-containing cytochalasin derivative is obtained by fermenting and culturing the fungus Phomopsis sp. QYM-13 from its culture. The separation method and identification results of the iodine-containing cytochalasin derivatives are shown below.

[0044] 1. Separation of iodine-containing cytochalasin derivatives, the specific steps are as follows:

[0045] (1) Seed culture of the fungus Phomopsis sp.QYM-13:

[0046] The fungus Phomopsis sp.QYM-13 described in Example 1 was picked and inserted into the slant medium, and cultured at 25-27°C for 3-5 days; in this example, the culture was at 25°C for 3 days.

[0047]The weight ratio formula of the slant medium is: yeast extract 0.1%, agar 1.5%-2.5%, sodium chloride 1.5%-4%, peptone 0.1%-0.5%, water 93-98%; glucose 0.3%; Make a test tube bevel.

[0048] (2) Fermentation culture of fungus Phomopsis...

Embodiment 3

[0060] Example 3 Activity test of iodine-containing cytochalasin derivative indophomopcalasin

[0061] 1. Experimental materials:

[0062] RAW264.7 macrophages were purchased from the Cell Bank of the Type Culture Collection, Chinese Academy of Sciences; 96-well cell culture plate (Corning); modified Eagle medium (DMEM, Solarbio); penicillin-streptomycin mixture (Solarbio); Australian fetal bovine Serum (FBS, Gibco); lipopolysaccharide (LPS, Sigma); PBS buffer (Boster); thiazole blue (MTT, Hualan Chemical); dimethyl sulfoxide (DMSO, Sigma); RIPA cell lysate (weak ) (Biyuntian Biotechnology); Nitric Oxide Kit (Nanjing Jiancheng Bioengineering Institute).

[0063] 2. Test content

[0064] (1) Cell culture: mouse macrophage cell line RAW 264.7 cells were cultured in DMEM high glucose medium containing 10% fetal bovine serum (FBS), penicillin (100U / mL) streptomycin (100μg / mL) mixture , placed at 37°C, 5% CO 2 The cells were cultured in an incubator, and when the cell fusion re...

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Abstract

The invention discloses an iodine-containing cytochalasin derivative derived from marine fungi as well as a preparation method and application of the iodine-containing cytochalasin derivative. According to the invention, a strain of marine mangrove forest endophytic fungus Phomopsis sp.QYM-13 is obtained by screening from healthy leaves of a mangrove plant Kandelia candel, and an iodine-containing cytochalasin derivative inphomopchalasin with anti-inflammatory activity is extracted from the marine mangrove forest endophytic fungus Phomopsis sp.QYM-13. The invention not only provides a new drug source for the preparation of anti-inflammatory drugs, but also has the advantages of simple preparation method and low cost, and is suitable for large-scale industrial application.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to a marine fungus-derived iodine-containing cytochalasin derivative and its preparation method and application. Background technique [0002] Inflammation is a defense response of living tissues with vascular system to damaging stimuli, and plays an extremely important role in the occurrence and development of many major diseases in the human body. Moderate inflammatory response is beneficial to body tissues, while excessive inflammatory response will destroy normal cells and tissues and induce various acute and chronic inflammatory diseases, such as arthritis, atherosclerosis, asthma, tumors, ulcerative colitis, Diabetes and coronary heart disease, etc., seriously endanger human health. There are currently two types of anti-inflammatory drugs commonly used in clinical practice: non-steroidal anti-inflammatory drugs and steroidal anti-inflammatory drugs. Howe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C07D491/06A61K31/407A61P29/00C12R1/645
CPCC12N1/145C12P17/18C07D491/06A61P29/00C12R2001/645Y02A50/30
Inventor 佘志刚陈岩杨文聪
Owner SUN YAT SEN UNIV
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