Culture medium capable of improving osteogenic differentiation efficiency of human mesenchymal stem cells and culture method

Abstract

The invention discloses a culture medium capable of improving osteogenic differentiation efficiency of human mesenchymal stem cells and a culture method. The culture medium is prepared by periodically adding cytochalasin D into a traditional human stem cell osteogenic differentiation culture medium. The culture method comprises an early-stage culture medium and a later-stage culture medium, wherein the early-stage culture medium contains cytochalasin D. The early-stage culture medium is used for the first 14 days of human mesenchymal stem cell in-vitro osteogenesis induction, and the later-stage culture medium is used for 14-28 days of in-vitro osteogenesis induction. By adopting the osteogenic induction culture solution, the alizarin red dyeing effect can be remarkably improved, meanwhile, the expression of osteogenic related genes and proteins COL1A1 and OCN in the later period of osteogenic differentiation of the human mesenchymal stem cells is remarkably promoted, and the results show that the osteogenic differentiation efficiency of the human mesenchymal stem cells can be remarkably improved, and the result indicates that the method can be used for bone tissue regeneration and repair in stem cell treatment.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a culture medium and a culture method capable of improving the osteogenic differentiation efficiency of human mesenchymal stem cells. Background technique [0002] Human mesenchymal stem cells are obtained from tissues such as bone marrow, fat, and umbilical cord, and can be expanded in vitro and differentiated into multiple lineages such as chondrocytes and osteoblasts. The application of human mesenchymal stem cells not only eliminates ethical issues, but also provides a new method for the regeneration and repair of tissues such as bone, cartilage, muscle and nerve. However, the non-directed differentiation of stem cells in vitro and in vivo can lead to the formation of unwanted cells, and even have certain tumorigenicity, which poses challenges to the clinical application of stem cells in the treatment of bone defects. Therefore, it is necessary to further im...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low osteogenic differentiation efficiency of human mesenchymal stem cells at the present stage, the present invention provides a medium and a culture method that can improve the osteogenic differentiation efficiency of human mesenchymal stem cells, using cytochalasin D to regulate the mesenchymal stem cell skeleton in the differentiation process Mechanical properties, thereby further improving the osteogenic differentiation efficiency of human mesenchymal stem cells

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