Preparation method for mesoporous bioactive glass/sodium alginate-sodium alginate layered bone tissue engineering scaffold

A technology of bone tissue engineering and sodium alginate, applied in the field of materials science, can solve the problems of poor osteoconductivity, fast drug release, low mechanical strength, etc. Effect

Active Publication Date: 2018-12-07
UNIV OF SHANGHAI FOR SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems in the prior art, the present invention provides a method for preparing a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold. The preparation of simple sodium alginate stents in the prior art has problems such as low mechanical strength, poor bone conduction, and fast drug release.

Method used

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  • Preparation method for mesoporous bioactive glass/sodium alginate-sodium alginate layered bone tissue engineering scaffold
  • Preparation method for mesoporous bioactive glass/sodium alginate-sodium alginate layered bone tissue engineering scaffold
  • Preparation method for mesoporous bioactive glass/sodium alginate-sodium alginate layered bone tissue engineering scaffold

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Embodiment 1

[0028] A method for preparing a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold, comprising the following steps:

[0029] Step 1, preparation of mesoporous bioglass powder: bioactive glass blocks with good mesoporous channels (molar ratio SiO) are prepared by volatilization-induced self-assembly method. 2 :CaO:P 2 O 5 =80:15:5), then ball-milled the mesoporous bioglass blocks, passed through a 400-mesh sieve, the particle size was less than 37 microns, and dried for later use.

[0030] Step 2, preparing sodium alginate layer ink: dissolving sodium alginate in deionized water (25% by mass), and magnetically stirring to obtain a uniformly mixed sodium alginate ink for subsequent three-dimensional printing.

[0031]Step 3, prepare mesoporous bioglass / sodium alginate layer composite ink: dissolve sodium alginate in deionized water to obtain a solution with a mass fraction of 10%, and add the mesoporous bioglass powder obtained in step...

Embodiment 2

[0036] Example 2 Drug Loading and Release Example

[0037] A preparation method of a drug-loaded mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold, comprising the following steps:

[0038] Step 1, prepare the sodium alginate layer ink loaded with bovine serum albumin (BSA): dissolve the BSA in deionized water to form a uniform solution, and then dissolve the sodium alginate in it to obtain the sodium alginate layer ink loaded with BSA, The mass fraction of sodium alginate is 25%, and the mass fraction of BSA is 2%.

[0039] Step 2, prepare the mesoporous bioglass / sodium alginate layer composite ink loaded with ibuprofen drug (IBU): dissolve the IBU in a small amount of absolute ethanol, and then add deionized water to make the concentration 50 mg / ml. Next, 2.0 g of mesoporous bioglass powder was added to 100 ml of IBU solution and stirred at room temperature for 24 hours, and then centrifuged. The upper layer was taken to measure its...

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Abstract

The invention provides a preparation method for mesoporous bioactive glass / sodium alginate-sodium alginate layered bone tissue engineering scaffold. The preparation method includes a step of preparingsodium alginate ink; a step of preparing mesoporous bioactive glass / sodium alginate composite ink; a step of designing the appearance and internal structure of a mesoporous bioactive glass / sodium alginate-sodium alginate layered bone tissue engineering scaffold; and a step of preparing the mesoporous bioactive glass / sodium alginate-sodium alginate layered bone tissue engineering scaffold by adopting a three-dimensional printing technology. Proved by cell experiment results, the prepared mesoporous bioactive glass / sodium alginate-sodium alginate layered scaffold has excellent capabilities of promoting proliferation and differentiation of human bone marrow mesenchymal stem cells (hBMSCs), and therefore, excellent biological activity can be shown.

Description

technical field [0001] The invention belongs to the field of materials science, and relates to a bone tissue engineering scaffold, in particular to a preparation method of a mesoporous bioglass / sodium alginate-sodium alginate layered bone tissue engineering scaffold. Background technique [0002] At present, bone tissue defects caused by tumor resection, congenital diseases, infection and trauma are common clinical conditions. The use of bone tissue engineering scaffolds to repair the defect is considered to be one of the effective methods to solve related problems. Since a series of infection, inflammation and pain problems are easily caused after bone transplantation, it is difficult for a simple scaffold material to meet the requirements of an ideal bone repair material. If the drug can be loaded directly into the implant material and released in situ after implantation, the in situ release of the drug can not only prevent the metastasis and spread of diseased cells, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/10A61L27/22A61L27/50A61L27/54A61L27/56B33Y10/00B33Y70/00
CPCA61L27/10A61L27/20A61L27/227A61L27/50A61L27/54A61L27/56A61L2300/252A61L2300/402A61L2300/404A61L2300/41A61L2300/412A61L2300/602A61L2430/02B33Y10/00B33Y70/00C08L5/04C08L89/00
Inventor 朱钰方杜晓宇胡亚萍
Owner UNIV OF SHANGHAI FOR SCI & TECH
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