Cell membrane microvesicles targeting inflammation region and application of cell membrane microvesicles

A technology of cell membranes and microvesicles, which is applied in the direction of medical preparations with non-active ingredients, anti-inflammatory agents, active ingredients of ketones, etc. It can solve the problems of not having high-efficiency targeting inflammatory areas, and maintain stability and biological activity , highly effective anti-inflammatory effect

Inactive Publication Date: 2021-08-17
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to solve the technical problem that the existing materials do not have the characteristics of efficiently targeting inflammation areas, and provide a cell membrane microvesicle with targeting inflammation areas and its application

Method used

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  • Cell membrane microvesicles targeting inflammation region and application of cell membrane microvesicles
  • Cell membrane microvesicles targeting inflammation region and application of cell membrane microvesicles
  • Cell membrane microvesicles targeting inflammation region and application of cell membrane microvesicles

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Embodiment Construction

[0021] The present invention will be further described below in conjunction with the examples.

[0022] The sources of biological materials used in the examples of the present invention are as follows: 1. CXCR4 gene overexpression lentivirus: Gnenchem, Shanghai, China; 2. MC-3T3 cells, Raw264.7 cells: Cyagen Biosciences Inc. (USA); 3. Cell relaxation Element B: Solarbio, Beijing, China.

[0023] The present invention aims to solve the problem of insufficient targeting of existing drug carriers to inflammatory areas, and provides a preparation method and application of a bio-based drug carrier capable of loading curcumin and targeting the inflammatory area to exert an anti-inflammatory effect.

[0024] Using genetic engineering, MC-3T3 cells were transfected at a multiplicity of infection (MOI) of 50 by overexpressing CXCR4 gene lentivirus, and the expression of GFP was observed under a fluorescent microscope. After 72 hours of transfection, the fluorescence intensity reached ...

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Abstract

The invention relates to a cell membrane microvesicle targeting an inflammation region and application of the cell membrane microvesicle, and solves the technical problem that an existing material does not have the characteristic of efficiently targeting the inflammation region. A preparation method of the cell membrane microvesicle comprises the following steps: transfecting an MC-3T3 cell through CXCR4 gene overexpressing lentivirus by utilizing genetic engineering, and proliferating the transfected cell to obtain cells with overpressed membrane receptor CXCR4; culturing the over-expressed CXCR4 MC-3T3 cells, conducting washing, conducting treating with cytochalasin B, and carrying out vortex separation on the cells and cell membrane microvesicles; centrifuging the mixture, separating the cells from the cell membrane microvesicles, collecting supernate, and conducting centrifuging for the second time to obtain the cell membrane microvesicles of the targeted inflammation region. The invention also provides an application of the cell membrane microvesicle of the targeted inflammation region in preparation of a targeted inflammation region material. The method can be used in the field of preparation of drug loading materials.

Description

technical field [0001] The present invention relates to a biological material and its application, in particular to a cell membrane microvesicle targeting inflammation area and its application. Background technique [0002] Inflammation is closely related to various chronic diseases, such as inflammatory bowel disease (IBD), autoimmune diseases, and cancer, but the treatment of inflammation-related diseases remains a daunting challenge in clinical treatment. Despite the development of many anti-inflammatory drugs over the past few decades, systemic adverse effects from drug overdose remain a vexing problem. Therefore, in order to improve the bioavailability and effectiveness of drugs, many scholars have explored drug delivery systems. [0003] Cell membrane-coated carriers are a drug delivery carrier that evades the immune system by camouflaging drug particles. However, the cell membrane may be damaged during the membrane coating process, and its long-term safety and stabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61K47/69A61K47/46A61P29/00
CPCA61K31/12A61K47/6901A61K47/46A61P29/00
Inventor 邓旭亮夏斌王丹丹
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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