2results about How to "Realize large-scale preparation" patented technology

The application belongs to the technical field of bio-chemical industry, and particularly relates to a P450 fusion protein, and a structure of the fusion protein is VdhM3-Linker1-AciC-Linker2-AciB, wherein VdhM3 is Vdh-M3 (I114R / N173M / Q310R); the GenBank accession numbers of the encoding genes of AciC and AciB are both AB221118.1; the amino acid sequence of Linker2 is GSGSGH; and Linker1 is a flexible linker, a rigid linker or a flexible-rigid combined linker. Through systematic screening, it is determined that the AciC / AciB oxidation-reduction partner system derived from Acinetobacter has the best adaptability with the VdhM3 P450 enzyme, the catalytic activity is high, and the selectivity of the target product calcifediol is as high as 95%, the generation of by-products is reduced, and the purification efficiency and yield of the product are improved.

The application relates to the field of oil and gas field fracturing technology, and particularly discloses an ultra-low-density micro-nano proppant for unconventional reservoir fracturing and a preparation method thereof. The proppant is prepared from a mixture containing 30-60 wt% of a vinyl monomer, 10-25 wt% of an acrylic ester monomer and 25-50 wt% of an unsaturated resin, emulsified into micro-nano scale droplets through a compounded emulsifier system, and then solidified. The preparation method comprises the following steps: mixing the resin composition with the emulsifier to form a uniform oil phase, dispersing the oil phase into an aqueous phase under high-speed shearing to form a micro-nano emulsion; and then realizing complete solidification of the droplets under mild conditions through an oxidation-reduction solidification system. The proppant has ultra-low density and micro-nano scale particle size, can effectively enter and support a super-remote complex micro-nano scale fracture network in an unconventional reservoir, and greatly improves the oil and gas recovery rate. The proppant has a complete structure under a 60MPa closed pressure, and can significantly improve the fracture conductivity.