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Cytochalasin compound and preparation method and use thereof

A kind of compound, technology of use, application in the field of use in the preparation of cell proliferation inhibitor or antitumor agent

Inactive Publication Date: 2011-11-30
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study found that the compound shown has anti-tumor activity, but there is no report on the chemical structure and cell proliferation inhibitory activity of the compound, so there is no related drug on the market

Method used

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  • Cytochalasin compound and preparation method and use thereof
  • Cytochalasin compound and preparation method and use thereof
  • Cytochalasin compound and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Fermentative production and separation and purification of the compound of embodiment 1

[0012] 1 Fermentation production

[0013] Fermentation culture of production bacteria: according to the conventional method of cultivating microorganisms, an appropriate amount of Aspergillus flavipes ZHN1-09 (Aspergillus flavipes ZHN1-09) (preservation number: CCTCC M 2011069) was taken, inoculated onto PDA solid slant medium, and cultivated at 28 degrees Celsius 6 days in the box.

[0014] Take an appropriate amount of Aspergillus flavipes ZHN1-09 (Aspergillus flavipes) cultured on a slant for 6 days, and inoculate it into 300mL of culture medium [medium composition (g / L): glucose 10.0, maltose 20.0, mannitol 20.0, monosodium glutamate 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, yeast extract 3.0, corn flour 0.3, pH=6.5] in a 1000mL Erlenmeyer flask, cultured at 28°C for 40 days to obtain mycelium and fermentation broth.

[0015] 2 Obtaining the extract

[0016] Separate the mycelium...

Embodiment 2

[0026] The test of embodiment 2 in vitro antitumor activity

[0027] Cell Proliferation Inhibitory Activity Test

[0028] 1 Experimental samples and experimental methods

[0029] Preparation of test sample solution The test sample is the pure product of the compound isolated and refined in the above-mentioned Example 1. Accurately weigh an appropriate amount of sample, and prepare a solution with the required concentration with methanol for activity measurement.

[0030] Cell lines and subculture of cells adopt cancer cell lines such as human lung cancer A549 cells, human leukemia HL60 cells and mouse leukemia P388 cells. All kinds of cells were subcultured in RPMI-1640 medium containing 10% FBS at 37°C in an incubator filled with 5% carbon dioxide.

[0031] Cell Proliferation Inhibitory Activity Test Method

[0032] The human lung cancer A549 cells in the logarithmic growth phase were prepared by the Lissamine rhodamine B (SRB) method with fresh RPMI-1640 medium to a dens...

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Abstract

The invention relates to a preparation method and a purpose of a cytochalasin compound. According to the invention, a novel cytochalasin compound is produced by using Aspergillus flavipes ZHN1-09 in a Guangxi mangrove forest plant Cerbera manghas root mug sample. It is proved by experiments that the compound can be used as cell proliferation inhibitors or antitumor agents.

Description

Technical field: [0001] The present invention relates to the production of cytochalasin compounds by Aspergillus flavipes ZHN1-09 ((preserved in China Center for Type Culture Collection of Wuhan University on March 24, 2011, preservation number: CCTCC M 2011069)) The method; the present invention also relates to the use of this type of compound in the preparation of cell proliferation inhibitors or antitumor agents. Background technique: [0002] Cytochalasin is a structurally and activity-related secondary metabolite produced by fungi, and scientists have discovered that this class of compounds has many unusual and interesting effects on cells. The inventors have studied and learned that the crude extract of the liquid fermentation product of Aspergillus flavipes ZHN1-09 (Aspergillus flavipes ZHN1-09) (preservation number: CCTCC M 2011069) has good cell proliferation inhibitory activity after ultrasonic crushing. Its active ingredients were studied. The study found that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/20C12P17/18A61P35/00A61P35/02C12R1/66
Inventor 李德海周会楠朱天骄顾谦群
Owner OCEAN UNIV OF CHINA
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