Induction method of high-heterozygosity tetraploid of new variety of crassostrea gigas 'Haida No.3'

Abstract

The invention provides an induction method of a high-heterozygosity tetraploid of a new variety of crassostrea gigas 'Haida No.3'. The method comprises the following steps: taking female triploid crassostrea gigas 'Haida No.3' obtained by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas 'Haida No.3' as a female parent; taking a male diploid 'Haida No.3' as a male parent; after the female parent and the male parent are artificially matured, putting ova into seawater to be matured, and when the ova are round through microscopic examination, the ova are matured; conducting insemination on the cured ova and sperms, starting timing after the sperms and the ova are mixed, treating fertilized ova with cytochalasin B, inhibiting discharging of the first polar bodies of the fertilized ova, and collecting and soaking the fertilized ova; and transferring the fertilized ova into a cultivation container for incubation and larva cultivation. The triploid female parent shellfish is obtained by inhibiting the discharge of the first polar bodies of the fertilized ova of the diploid crassostrea gigas 'Haida No.3', and the tetraploid obtained by the triploid female parent shellfish has higher growth advantage due to higher gene heterozygosity.

Description

technical field [0001] The invention belongs to the technical field of shellfish breeding, and relates to a tetraploid induction method of long oyster, in particular to a high heterozygosity tetraploid of a new variety of long oyster "Haida No. 3" with pure black shell color and rapid growth advantages method of induction. Background technique [0002] Long oyster, also known as Pacific oyster, is the main species of oyster culture in northern my country. At the same time, due to its strong environmental adaptability, fast growth rate, and rich nutrition, it has become the most widely cultivated economic shellfish in the world. For a long time, due to the large amount of oyster spawning, the parents used for seed production are limited and all come from unselected breeding populations. There have been germplasm degradation phenomena such as reduced genetic diversity and individual miniaturization of the breeding population, which has restricted The healthy and sustainable d...

AI Technical Summary

Problems solved by technology

There are two main ways to obtain triploid oysters. One is to induce triploids directly through the sperm eggs of diploid oysters. However, this method has unstable triploid rate and is difficult to reach 100%, so it is difficult to apply on a large scale. The second is that tetraploid oysters and diploid oysters are hybridized to obtain triploids. This method can theoretically obtain 100% triploid offspring, and tetraploids can expand the tetraploid population by self-propagation, which is three times Provides a guarantee for commercial production of the body

Another method is to use the sperm and eggs of diploid oysters to directly induce tetraploid oysters. The triploid offspring produced by hybridization between tetraploids and diploids obtained by this method are more sterile, theoretically It has a higher growth advantage, but this method also has problems such as relatively low tetraploid induction rate and low survival rate

The most commonly used way to obtain triploid oysters is by inhibiting the second polar body of diploid oyster fertilized eggs. At present, the triploids used to induce tetraploid oysters are basically obtained by inhibiting the second polar body of diploid fertilized eggs. Yes, tetraploids suffer from slower growth compared to diploids and triploids