Cultivation method for rapidly growing triploid crassostrea gigas Haida No.1

Abstract

The invention provides a cultivation method for rapidly growing triploid crassostrea gigas Haida No.1. The method comprises the following steps: obtaining high-heterozygosity triploid crassostrea gigas Haida No.1 by inhibiting discharge of first polar bodies of fertilized eggs of diploid crassostrea gigas Haida No.1, taking females of the triploid crassostrea gigas Haida No.1 as female parents, taking males of the diploid Haida No.1 as male parents, and obtaining tetraploid crassostrea gigas Haida No.1 with improved growth characters by inhibiting discharge of the first polar bodies of the fertilized eggs; and hybridizing males of the tetraploid crassostrea gigas Haida No.1 and the diploid crassostrea gigas Haida No.1 to obtain the triploid crassostrea gigas Haida No.1 which grows quickly. According to the method, the growth performance of the crassostrea gigas is improved by adopting a method for improving the heterozygosity of parent genes, and the tetraploid crassostrea gigas Haida No.1 obtained by adopting the method is hybridized with the diploid crassostrea gigas Haida No.1 which originally has the characteristic of rapid growth to obtain the triploid crassostrea gigas Haida No.1 with rapid growth performance.

Description

technical field

[0001] The invention belongs to the technical field of shellfish breeding, and relates to a method for cultivating triploid elongated oysters, in particular to a method for cultivating the triploid elongated oyster "Haida No. 1" with rapid growth. Background technique

[0002] Long oyster, also known as Pacific oyster, is the main species of oyster culture in northern my country. At the same time, due to its strong environmental adaptability, fast growth rate, and rich nutrition, it has become the most widely cultivated economic shellfish in the world. Oyster culture in my country is mainly based on diploid, but with the application and development of polyploid technology, triploid oyster culture has begun to rise in my country in recent years, which is mainly due to the gonad development block of triploid oyster, which solves the problem of It solves the problem that diploid oysters are difficult to supply to the market due to the thinning of the soft body af...

Examples

Embodiment 1

[0025] A method for cultivating fast-growing triploid elongated oyster "Haida No. 1", comprising the following steps:

[0026] (1) Select the 2-year-old diploid long oyster "Haida No. 1" with a shell height of 10-12 cm as the male and female broodstock respectively. Eggs were collected and filtered with 800-mesh sieve to remove tissue fragments and interstitial fluid, and then the eggs were added to sand-filtered seawater at 24-25°C for maturation. Start timing after the sperm and eggs are mixed, and 5 minutes after insemination, treat with 0.5 mg / L cytochalasin B, and finish the treatment for 15 minutes or when the first polar body begins to appear.

[0027] (2) Use flow cytometry to conduct non-lethal ploidy detection on the second-year-old triploid elongated oyster "Haida No. 1" obtained in (1), and select eggs with a large number of eggs (at least 1 million) and The individual with better oval shape was used as the female parent, and the eggs were dissected and filtered t...

Embodiment 2

[0033] A method for cultivating fast-growing triploid elongated oyster "Haida No. 1", comprising the following steps:

[0034] (1) Select the 2-year-old diploid long oyster "Haida No. 1" with a shell height of 10-12 cm as the male and female broodstock respectively. Eggs were collected and filtered with 800-mesh sieve to remove tissue fragments and interstitial fluid, and then the eggs were added to sand-filtered seawater at 24-25°C for maturation. Start timing after the sperm and eggs are mixed, and 5 minutes after insemination, treat with 0.5 mg / L cytochalasin B, and finish the treatment for 15 minutes or when the first polar body begins to appear.

[0035] (2) Use flow cytometry to conduct non-lethal ploidy detection on the second-year-old triploid elongated oyster "Haida No. 1" obtained in (1), and select eggs with a large number of eggs (at least 1 million) and The individual with better oval shape was used as the female parent, and the eggs were dissected and filtered t...

technical field

[0001] The invention belongs to the technical field of shellfish breeding, and relates to a method for cultivating triploid elongated oysters, in particular to a method for cultivating the triploid elongated oyster "Haida No. 1" with rapid growth. Background technique

[0002] Long oyster, also known as Pacific oyster, is the main species of oyster culture in northern my country. At the same time, due to its strong environmental adaptability, fast growth rate, and rich nutrition, it has become the most widely cultivated economic shellfish in the world. Oyster culture in my country is mainly based on diploid, but with the application and development of polyploid technology, triploid oyster culture has begun to rise in my country in recent years, which is mainly due to the gonad development block of triploid oyster, which solves the problem of It solves the problem that diploid oysters are difficult to supply to the market due to the thinning of the soft body af...

Examples

Embodiment 1

[0025] A method for cultivating fast-growing triploid elongated oyster "Haida No. 1", comprising the following steps:

[0026] (1) Select the 2-year-old diploid long oyster "Haida No. 1" with a shell height of 10-12 cm as the male and female broodstock respectively. Eggs were collected and filtered with 800-mesh sieve to remove tissue fragments and interstitial fluid, and then the eggs were added to sand-filtered seawater at 24-25°C for maturation. Start timing after the sperm and eggs are mixed, and 5 minutes after insemination, treat with 0.5 mg / L cytochalasin B, and finish the treatment for 15 minutes or when the first polar body begins to appear.

[0027] (2) Use flow cytometry to conduct non-lethal ploidy detection on the second-year-old triploid elongated oyster "Haida No. 1" obtained in (1), and select eggs with a large number of eggs (at least 1 million) and The individual with better oval shape was used as the female parent, and the eggs were dissected and filtered t...

Embodiment 2

[0033] A method for cultivating fast-growing triploid elongated oyster "Haida No. 1", comprising the following steps:

[0034] (1) Select the 2-year-old diploid long oyster "Haida No. 1" with a shell height of 10-12 cm as the male and female broodstock respectively. Eggs were collected and filtered with 800-mesh sieve to remove tissue fragments and interstitial fluid, and then the eggs were added to sand-filtered seawater at 24-25°C for maturation. Start timing after the sperm and eggs are mixed, and 5 minutes after insemination, treat with 0.5 mg / L cytochalasin B, and finish the treatment for 15 minutes or when the first polar body begins to appear.

[0035] (2) Use flow cytometry to conduct non-lethal ploidy detection on the second-year-old triploid elongated oyster "Haida No. 1" obtained in (1), and select eggs with a large number of eggs (at least 1 million) and The individual with better oval shape was used as the female parent, and the eggs were dissected and filtered t...