14 results about "Charales" patented technology

The invention relates to the technical field of functional cosmetic raw materials, in particular to a cactus polysaccharide composition as well as a preparation method and application thereof. According to the cactus polysaccharide composition disclosed by the invention, the cactus polysaccharides with specific number-average molecular weight are combined according to a specific proportion, so that the cactus polysaccharide composition has a more comprehensive anti-irritation effect and has the characteristics of high stability and better skin feeling and solubility. The cactus polysaccharide can be prepared through a simple process, exogenous components do not need to be introduced, after the cactus aqueous extract is subjected to enzymolysis through amylase, the cactus polysaccharide with the specific number-average molecular weight is screened and combined according to the specific proportion, the anti-irritation effect requirement can be met, and safety is better. The cactus polysaccharide composition can be applied to cosmetics / pharmaceuticals as an anti-irritation raw material.

The present application belongs to the field of edible fungi, and particularly relates to a new species of Russula and application thereof. Pseudolagarobasidium acaciicola The taxonomic name of the new species of Russula is Pseudoguignardia sp., which has been registered and preserved in the Guangdong Microbial Culture Collection Center on April 24, 2022, and the preservation number is GDMCC No. 62414. The new species of Russula GDMCC No. 62414 provided in the present application can be artificially cultivated, and has the characteristics of large number of fruiting bodies, high yield, good mushroom shape and the like, and is a new species of Russula with great development potential.

The application discloses genetically engineered algae against aquatic pathogenic bacteria and a construction method and application thereof, characterized by that the genetically engineered algae is Phaeodactylum tricornutum which is introduced with cell wall enabling plasmid pPha-T1-EG01991-eGFP-Linker-Q4 or pPha-T1-EG01991-eGFP-Linker-Q6, the preparation method comprises the following steps: taking the genomic DNA of the Phaeodactylum tricornutum as a template, amplifying the Phatr3_EG01991 coding sequence to construct a directional expression plasmid pPha-T1-EG01991-eGFP, inserting the Q4 and Q6 coding sequences into the eGFP downstream of the directional expression vector to construct the cell wall enabling plasmid, electrotransforming the cell wall enabling plasmid into the Phaeodactylum tricornutum, and coating the transformed Phaeodactylum tricornutum cells on f / 2 flat plate culture medium for culture and screening positive algae strains, and the genetically engineered algae has the advantages of having the inhibiting or killing capacity to the aquatic pathogenic bacteria.

The application discloses a method for constructing a fast-growing specialized line of Litopenaeus vannamei. The method comprises the following steps: collecting and cultivating a fast-growing population, screening the fast-growing population, screening individuals in the fast-growing population for three rounds, cultivating zero-generation parents and breeding F1 generation, cultivating the F1 generation and breeding F2 generation, cultivating the F2 generation and breeding F3 generation, and dynamically maintaining the fast-growing specialized line. The shrimp line cultivation method of the application does not need to construct a family line, and has the characteristics of simplicity, high efficiency and low cost, thereby laying a technical foundation for large-scale and extensive breeding of the fast-growing trait of Litopenaeus vannamei.

This invention belongs to the field of biotechnology, specifically relating to a non-harmful DNA extraction method for *Scylla serrata* (scuttica crab) and its application. This invention successfully achieves non-harmful DNA extraction from *Scylla serrata* by collecting its swimming leg setae, significantly reducing the impact on the crab's activity and ensuring its survival after sampling. The extraction method of this invention is non-lethal to *Scylla serrata* and possesses good repeatability and high accuracy. Extracting DNA from *Scylla serrata* using its swimming leg setae ensures that the crab's activity remains unchanged, allowing it to survive and perform its life activities normally. Obtaining the DNA sequence of *Scylla serrata* and performing gene markers provides a foundation for genomic selection breeding, facilitating parent selection and offspring breeding in mud crab genetics.

The invention belongs to the technical field of ecological risk assessment, and particularly relates to an antibiotic ecological risk assessment method and system based on blue algae and green algae coupling. Monitoring the antibiotic concentration in the water environment to be detected; respectively carrying out growth inhibition experiments on the green algae and the blue-green algae under a set antibiotic concentration gradient, and respectively obtaining half effect concentrations of the blue-green algae and the blue-green algae according to a relation curve of algae density and antibiotic concentration; deriving according to a Hill function to obtain a relational expression between the exposure concentration and the growth rate of the green algae or the blue algae, and calculating the exposure concentration of the antibiotics in combination with a mixed growth rate formula; and finally evaluating the ecological risk level of the antibiotics in the water environment according to a risk entropy method. Compared with a single species assessment method, the method can more truly reflect the response characteristics of the algae community in the natural water to the antibiotics, and provides a scientific basis for antibiotic ecological risk judgment and environmental threshold formulation.

The invention discloses a phaeodactylum tricornutum high-temperature-resistant engineering algal strain and a construction method and application thereof, and is characterized in that the high-temperature-resistant engineering algal strain is phaeodactylum tricornutum of an overexpressed bd1311 gene, and the nucleotide sequence of the bd1311 gene is as shown in SEQ ID NO.1. The construction method of the algal strain comprises the following steps: by using pPha-T1 as a carrier, carrying out high-temperature-resistant engineering on the phaeodactylum tricornutum, and carrying out high-temperature-resistant engineering on the carrying out connection fusion on seamless cloning based on homologous recombination and the full length of the sequence of the bd1311 gene to obtain a bd1311 gene overexpression vector; designing a bd1311 gene overexpression amplification primer, carrying out amplification by taking cDNA of wild phaeodactylum tricornutum as a template to obtain a target fragment, and carrying out recombinant cloning on the target fragment and the enzyme-digested linearized vector; performing escherichia coli transformation on the recombinant vector, and then electrically transforming the recombinant vector into phaeodactylum tricornutum to obtain a phaeodactylum tricornutum high-temperature-resistant engineering algal strain; the fucoxanthin gene has the advantages that the fucoxanthin gene is resistant to high temperature in summer and is positively regulated and controlled.

The present application relates to a kind of penaeus vannamei ovipositor breeding ponds, including several ovipositor breeding pond bodies, the upper portion in ovipositor breeding pond body is equipped with for the reproduction of seed shrimp spawning space, the lower portion of spawning space is equipped with for the hatching of shrimp eggs ovipositor space, the bottom of ovipositor space is equipped with one side with pool wall form the ovipositor bottom plate of positionable hinged, ovipositor bottom plate includes upper portion of egg-blocking net layer, middle portion of aeration layer, lower portion of water-permeable net layer, aeration layer is equipped with multiple aeration pipes, aeration pipe is communicated with external air source, aeration pipe is distributed with aeration hole, the lower portion of ovipositor space is equipped with for the cultivation of fry breeding space, the bottom of breeding space is equipped with fry outlet on one side.The present application uses the way of pond bottom aeration to replace the existing artificial ovipositor and mechanism ovipositor mode, integrates the hatching and fry function of prawn on the ovipositor breeding pond body, with better ovipositor effect, breeding yield is improved, breeding cost is reduced, and ovipositor breeding efficiency is significantly improved, etc.

The invention belongs to the technical field of carbon nanomaterials, and discloses carbon quantum dots based on carya illinoensis shells as well as a preparation method and application of the carbon quantum dots. According to the carbon quantum dot, carya illinoensis shell powder is adopted as a carbon source, and the carya illinoensis shell powder and PEI are subjected to a hydrothermal method reaction in an aqueous solution to prepare a reaction product containing carbon quantum dot nanoparticles. The functionalized carbon quantum dot nanomaterial has the characteristics that the nanomaterial has a good protection effect on dsRNA, and the occurrence of apocarya anthracnose can be remarkably inhibited through the expression of pathogenic genes of spray-induced gene silencing colletotrichum gloeosporioides. And a new method is provided for green prevention and control of the disease.

The invention discloses a comprehensive planting and breeding method for rice, ducks and shrimps, and belongs to the field of agricultural production.The comprehensive planting and breeding method for rice, ducks and shrimps specifically comprises the steps that freshwater shrimps and macrobrachium rosenbergii are bred in a rice field in the first half year, and rice is planted and sheldrakes are bred in the second half year. According to the comprehensive planting and breeding method, the omnivorous characteristics of the freshwater shrimps and the macrobrachium rosenbergii are fully utilized, and the number of algae and other aquatic organisms in the rice field is reduced. Excrement of the shrimps can increase soil fertility and reduce the use amount of chemical fertilizer in the rice field; the ducks can prey on duckweed, aquatic plants and rice diseases and pests, eggs laid by the ducks can also increase income for farmers, and'five purposes in one water and five harvests in one field 'are achieved.

This invention discloses a strain of *Backusella circina* and its application. The strain number of *Backusella circina* is JSAFC 2057, which was deposited at the China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, with accession number CGMCC No. 41522. This fungal agent can significantly inhibit the survival activity of the fall webworm (*Triplophysa fulvidraco*), reducing the survival activity of fall webworm larvae to zero after 7 days of treatment and culture. The fungus provided by this invention is a biocontrol fungus for the control of the major pest, the fall webworm. It is characterized by strong pathogenicity against fall webworm larvae, environmental safety, and low likelihood of inducing pesticide resistance in pests, and can be applied to the biological control of the fall webworm.

The present application belongs to the technical field of ecological risk assessment, and specifically relates to an antibiotic ecological risk assessment method and system based on coupling of blue-green algae and green algae. The concentration of antibiotics in a water environment to be detected is monitored; growth inhibition experiments of green algae and blue-green algae are respectively carried out under a set antibiotic concentration gradient; a half effect concentration of the blue-green algae and the green algae is respectively obtained according to a relationship curve between algal density and antibiotic concentration; a relationship formula between an exposure concentration and a growth rate of the green algae or the blue-green algae is derived according to a Hill function; and the antibiotic exposure concentration is calculated in combination with a mixed growth rate formula; and finally, the ecological risk grade of the antibiotic in the water environment is evaluated according to a risk entropy value method. Compared with a single species evaluation method, the present application can more truly reflect the response characteristics of algal communities in natural water bodies to antibiotics, and provides a scientific basis for antibiotic ecological risk judgment and environmental threshold setting.

This invention relates to a Ganoderma lucidum strain L4905, its cultivation method, and its applications, belonging to the field of biotechnology. The aforementioned Ganoderma lucidum strain L4905 was deposited at the Guangdong Provincial Microbial Culture Collection Center on October 9, 2023, with accession number GDMCC No: 63866. This invention's Ganoderma lucidum strain L4905 exhibits a short growth cycle and stable characteristics. This strain possesses a high-gloss, lacquer-like fruiting body, a kidney-shaped cap, thick flesh, high yield of both fruiting bodies and spores, stable cultivation characteristics, and high polysaccharide content, making it suitable for substrate cultivation and promotion in most areas of Yunnan Province.

The invention discloses a guiding and controlling method and device for reducing early fish resource entrainment loss at a lateral water intake of a water guiding and transferring project, and belongs to the field of ecological protection of water conservancy projects. The method comprises the steps that anchoring piers are arranged on the two sides of a water intake, and angle adjusting systems are installed; the start and stop of the fish breeding period are automatically judged by continuously monitoring water temperature; when the breeding period begins, the flexible curtain wall is spread and installed on site through the traction control system; adjusting the height of the curtain wall according to the real-time water level; after the breeding period is finished, the device is recycled to the bank. The device comprises a flexible curtain wall, a top restraining structure, a bottom fixing structure, a traction control system and an angle adjusting system. According to the ecological characteristic that early fish resources drift along with flow, a traditional protection thought taking interception as a main part is changed, a regulation and control strategy taking dredging as a core is provided, and effective technical support is provided for implementation of eco-friendly scheduling of water diversion engineering.