43 results about "Colletotrichum dematium" patented technology

The invention provides a combined volatile compound and application thereof in preventing and treating anthracnose of postharvest mangos. According to the invention, the application of the combined volatile compound in preventing and treating the anthracnose of the postharvest mangos is found for the first time. The combined volatile compound can act on colletotrichum mycelium and conidium by volatilization in a gas mode without contacting the fruits in a sealed environment to inhibit mycelia growth, conidia germination and disease speckle expansion; thus, an effect of preventing and treatingthe mango anthracnose is achieved. As the combined volatile compound is extremely easy to volatilize, the compound left on the surfaces of the mangos after steaming treatment is easy to volatilize; thus, an effect on mango quality and harm to human body health are reduced. The invention determines matching and use concentrations of 7 groups of different combined volatile compounds with an effect of preventing and treating the mango anthracnose. Compared with existing preservation chemicals, the combined volatile compound has the advantages of good preventing effect, low residue and very good development and application prospects.

The present invention mainly provides a hydroxylamine compound and its preparation method which have inhibitory effects on Fusarium oxysporum, Fusarium moniliforme, Fusarium solani and anthracnose. Its chemical structural formula is: The plant disease caused by Fusarium oxysporum, Fusarium moniliforme, Fusarium solani and Anthracnose provides a new way, and the preparation method is simple, convenient and operable.

The invention discloses an antagonistic bacterium capable of increasing the pH value of acid soil and preparation and application of a microbial agent of the antagonistic bacterium. The antagonistic bacterium is bacillus amyloliquefaciens and is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO. 18619. The antagonistic bacterium has astrong antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium graminearum, colletotrichum gloeosporioides, alternaria alternata and botryosphaeria dothidea, can be stably colonized at rhizosphere of plants such as wheat, corn, pepper and poplar, and can remarkably increase the pH value of the acid soil and promote crop growth. The microbial agent prepared from the antagonistic bacterium can be applied to improvement of growth of plants such as wheat, corn, pepper and poplar in the acid soil and prevention and control of fungal diseases, and has effects of increasing the pH value of the acid soil, preventing diseases and promoting growth; and the preparation process of the microbial agent is simple, the fermentation period is short, the cost islow, and industrial production and transportation are facilitated.

The invention relates to a Trichoderma strain and its application in the agricultural field. A strain of Trichoderma, named Trichoderma harzianum, was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.15679 and the preservation date was May 21, 2018. The above-mentioned Trichoderma is used to inhibit the growth of Rhizoctonia solani, Anthracnose and Fusarium. The above-mentioned Trichoderma is used for soil phosphorus solubilization. The above-mentioned Trichoderma is used for preparing microbial fertilizer. A strain of Trichoderma disclosed by the present invention and its application in the agricultural field have the following beneficial effects: 1. Provide a bacterial strain for inhibiting Rhizoctonia solani, anthracnose, and Fusarium, which has a good effect on the above-mentioned plant harmful pathogens 2. The bacterial strain can also be used to prepare microbial seed coating agents, which can play a beneficial role in promoting the growth of plant roots without losing activity.

The invention belongs to the technical field of microorganisms, and specifically discloses a strain of Paenibacillus terreus YC16‑08 and its application. The preservation number of the Paenibacillus terreus YC16-08 is CGMCC No.16181. It has been found through experimental research that it is effective against Phytophthora capsici, Botrytis cinerea, Anthrax oxysporum, Rhizoctonia solani, Fusarium oxysporum, Both Fusarium solani and Verticillium have antagonistic effects. And the Paenibacillus terreus YC16-08 can produce siderophilic, amylase, protease, pectinase, cellulase, IAA and NH3, and has nitrogen fixing ability and phosphorus dissolving ability.

The invention discloses the use of volatile oil of clove; that is, its application in agriculture to prevent and treat root rot of Chinese medicinal materials or crops or to prevent and control pathogenic bacteria that cause diseases in the aboveground parts of Chinese medicinal materials or crops; experimental results It shows that the volatile oil of mother clove can inhibit the mycelial growth of the four main pathogenic bacteria Fusarium oxysporum, Fusarium solani, Pythium and Cylindrospora destroyer that cause root rot of Panax notoginseng, and can completely inhibit the growth of fungi that cause root rot of Panax notoginseng. The three kinds of pathogenic bacteria that occurred in some diseases inhibited the growth of mycelia of Botrytis cinerea, G. anthracnose and Rhizoctonia solani; The compounding of methazol has a synergistic effect on multiple strains; due to the volatile nature of volatile oil and its aromatic and anti-pollution characteristics, the chemical fertilizers or pesticides developed with it will have the advantages of safety, effectiveness, and low residue.

The invention discloses an indolemethylaniline compound and its application, specifically a substituted 4‑((1 H ‑indol‑3‑yl)methyl)‑ N ‑Methylaniline compounds and their applications. The compound is simple to prepare and exhibits good antifungal activity. The present invention adopts the method of potato agar containing medicine (PDA) to carry out the fungal anthracnose oxysporum and strawberry anthracnose to the synthesized compound 、 Botrytis cinerea 、 The bactericidal activity assay of Fusarium oxysporum, all compounds of embodiment 1~14 are to anthracnose oxysporum, strawberry anthracnose 、 Botrytis cinerea all showed certain inhibitory activity, and some compounds also had certain inhibitory effect on Fusarium oxysporum.

The invention discloses a strain of Streptomyces sp. B5017, which is characterized in that the preservation number is CCTCC NO: M2019648 and the classification name is Streptomyces sp. B5017. Tests show that the new marine-derived Streptomyces strain of the present invention has the effect of antagonizing various plant pathogenic bacteria, and is effective against banana anthracnose, mango anthracnose, pearl anthracnose, Phytophthora vinifera, and Fusarium oxysporum No. 4 race. , Banana Fusarium oxysporum No. 1 race or Alternaria have inhibitory effect. The fermented liquid prepared by Streptomyces 5017 of the present invention can effectively avoid the yellowing and wilting of leaves caused by Fusarium oxysporum No. 4 and No. 1 races in pot experiments, which proves that the Streptomyces 5017 can effectively prevent and treat banana fusarium wilt, It has good ecological and social benefits to enhance the disease resistance of bananas, and has a good application prospect as a biocontrol preparation.

The invention discloses broad-spectrum fungus-resistant bacillus and application thereof to preventing wheat scab and relates to bacillus. The preservation number of the bacillus (Bacillus sp.)DY26-010 is CCTCC NO:M2015407. Supernatant liquid of the bacillus is antagonistic to fusarium spp, gloeosporium musarum, paecilomyces variotii, sclerotinia sclerotiorum, alternaria longipes, trichoderma viride, rhizoctonia, mutualism alternaria, botrytis cinerea and colletotrichum dematium. Fermentative supernatant liquid of the bacillus DY26-010 has high antagonism on pathogenic bacteria and fusarium spp of wheat scab, the preventing effect can be 57.1% in a potted experiment of wheat scab, and the bacillus can be used for preparing the fermentative supernatant liquid applied to preventing wheat scab. A biological prevention method of wheat scab is provided, no chemical pesticide is used, the bacillus has the advantages of being non-toxic, not prone to forming a pesticide-resistant environment, biologically safe and the like, and application prospects are good.

The invention belongs to the field of biotechnology, and specifically discloses a set of SSR primer pairs for persimmon anthracnose fungus developed based on the genome of a related species and an application thereof. The SSR primer pair of the persimmon anthracnose fungus is based on the genome of a close relative of the persimmon anthracnose fungus—the fruit-bearing anthracnose fungus, and 30 pairs of SSR primers have been developed through MISA software. The screening of pathogens has obtained 15 pairs of SSR primers with polymorphisms. The primers for Kaki anthracnose bacteria developed by the present invention are new markers that exist stably and can be used for the analysis of genetic diversity and genetic differentiation of Kaki anthracnose bacteria. It can also be used in the identification of persimmon anthracnose species and the study of kinship, which is of great significance to the effective prevention and control of persimmon anthracnose.

The present disclosure relates to a method for isolating apoplast juice from plant tissues infected with pathogenic bacteria, said apoplast juice containing effector proteins secreted by said pathogenic bacteria, the method comprising: a, placing plant tissues infected with pathogenic bacteria in Carry out infiltration treatment under vacuum in the buffer solution to obtain the plant tissue after the infiltration treatment; b. Centrifuge the plant tissue after the infiltration treatment for the first time to obtain the first centrifugate; take the first centrifugation solution for the second Sub-centrifugation to collect the second centrifugate; wherein, in the plant tissue infected with pathogenic bacteria, the pathogenic bacteria are Fusarium graminearum, Fusarium verticillium, Fusarium oxysporum, Verticillium dahliae, Anthracnose graminearum , Magnaporthe oryzae, Botrytis cinerea at least one. This method can maximize the separation of the effector proteins secreted by pathogenic bacteria and enter into the apoplast juice, which facilitates better analysis of the components and characteristics of the apoplast effector proteins secreted by pathogenic bacteria in susceptible plant tissues.

The present invention relates to a Spinacia oleracea seed designated 51-521 RZ, which exhibits a combination of traits including fast growing, a flat plant habit, smooth mature leaves, a field resistance against Colletotrichum dematium f. sp. spinaciae and resistance to downy mildew (P. farinosa f. sp. spinaciae) races Pfs1 to Pfs15. The present invention also relates to a Spinacia oleracea plant produced by growing the 51-521 RZ seed. The invention further relates to methods for producing the spinach cultivar, represented by spinach variety 51-521 RZ.

The invention discloses a bacillus thuringiensis strain for biocontrol and its application. The preservation number of Bacillus thuringiensis BCN10 is CGMCC No: 16675. Its fermented liquid has an effective inhibitory effect on the common spoilage fungi (Fusarium oxysporum, Botrytis genus, Trichoderma dark green, Glososporum anthracnose, Penicillium expanses) on the surface of loquat on double-zone plates and loquat fruit; fermentation The volatile substances produced by the liquid have antiseptic application in loquat storage, which can delay the onset time of natural spoilage of loquat and reduce the incidence rate. The volatile antibacterial substances produced by Bacillus thuringiensis (Bacillus thuringiensis) BCN10 can not directly contact the fruit, realize the freshness of the fruit at room temperature, prolong the storage time of the food, and can be used to prepare a kind of antibacterial agent for inhibiting Fusarium oxysporum, grape seat cavity Bacteria, Trichoderma dark viridans, Glucosporum anthracnose, Penicillium expanses and other spoilage bacteria, bio-control preservatives with a wide antibacterial spectrum.

The invention discloses a strain of Paenibacillus jemira inhibiting anthracnose and Fusarium and its application. The present invention isolates a strain of Paenibacillus jamilae from deep-sea sediments. The strain has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on August 8, 2018, and the preservation number is CGMCC No. .16231. The bacterial strain has broad-spectrum resistance to various plant pathogenic bacteria, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by glyosporium anthracnose and vegetable wilt caused by fusarium oxysporum.

The invention discloses a method for inducing the production of conidia of anthrax bacteria, which belongs to the technical field of plant protection; it comprises the following steps: (1) activation of anthrax bacteria: inoculating anthrax bacteria on a culture medium for activation and culture, and obtaining the activated (2) Inducing spore production: take the activated anthrax bacteria in step (1) and put them into an oven for high-temperature culture, then take them out and place them in a constant temperature incubator at 27°C for constant temperature culture for 7 days, then the conidia can be induced Produce; the present invention carries out high-temperature culture first and then carries out constant temperature culture to anthrax bacteria first, realizes simple and large amount of induction anthrax bacteria to produce a large number of spores, for the pathogenicity determination of anthrax bacteria, its bactericide bioactivity determination and the identification of disease resistance of varieties etc. research is of great significance.

The invention discloses a panax notoginseng phytohormone conjugated protein gene PnPhBP1. The PnPhBP1 has a nucleotide sequence shown in SEQ ID NO: 1 and encodes protein with an amino acid sequence shown in SEQ ID NO: 2. According to the application, proven by functional genomics related technical studies, the gene PnPhBP1 has the function of improving fungal resistance of plants; the antifungal gene PnPhBP1 is constructed into a plant expression vector and is transferred into tobacco for overexpression, thus, transgenic nicotiana tabacum plants have very high in-vitro antifungal activity, andexperiment results display that transgenic nicotiana tabacum with PnPhBP1 overexpression plays an obvious role in inhibiting the growth of three kinds of fungi such as colletotrichum gloeosporioides,botrytis cinerea and fusarium solanum.

The invention belongs to the technical field of plant genetic engineering, and in particular relates to a karst anthracnose fungus and its application in the field of rubber anthracnose research. This application first discovered the occurrence of karst anthrax on rubber trees in Hainan Province, and the isolated strain was C.karstii. The test of biological characteristics found that the optimum growth temperature of the strain was 28°C, and the lethal temperature was 35°C; the optimum growth pH was 6; the alternation of light and dark was conducive to the growth of colonies; pectin and peptone were the optimum carbon and nitrogen sources respectively. The evaluation of pathogenicity found that it has strong pathogenicity against the four main varieties of PR107, RRIM600, Reyan 7‑33‑97 and Dafeng 95; (DMIs) The fungicide prochloraz manganese salt was not resistant, but showed high resistance to the benzimidazole fungicide carbendazim.