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Morinda citrifolia sterilization preparation and preparation method thereof

The technology of Morinda citrifolia and preparations is applied in the field of sterilizing preparations of Morinda citrifolia and its preparation, which can solve the problems of difference in the separation frequency of pathogenic bacteria, nearly circular shape, brown color, etc., and achieves improved bacteriostatic properties, high bactericidal activity, and increased antibacterial properties. The effect of bacterial performance

Active Publication Date: 2021-10-08
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Morinda officinalis is rich in nutrition and has high medicinal value. At present, my country’s Hainan and Yunnan have already started large-scale planting of Morinda officinalis. However, during the planting and cultivation process, the damage of diseases and insect pests threatens the growth of Morinda officinalis. It is found that anthracnose bacteria damage the leaves of Morinda officinalis, Fruits and tender stems, the lesion on the affected leaves is nearly round and light red at the initial stage, and the outer circle of the lesion is reddish brown; in the later stage, many dark brown cushion-shaped conidia discs grow on the lesion, and the center of the diseased tissue becomes dry and becomes Thin, papery; perforated at the end, and in severe cases, the lesions connect together to form larger lesions. After the fruit and tender stems are damaged, they often form slightly sunken, nearly round, brown lesions, and dark brown lesions grow later Cushion-like conidia discs; there is also stem rot, which is caused by Pythium, anthracnose, and Fusarium infection. The isolation frequency of pathogenic bacteria in different ecological zones is different, and the isolation frequency of pathogenic bacteria in the same ecological zone varies between years and regions. There are significant differences

Method used

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  • Morinda citrifolia sterilization preparation and preparation method thereof
  • Morinda citrifolia sterilization preparation and preparation method thereof
  • Morinda citrifolia sterilization preparation and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A bactericidal preparation of Morinda officinalis: comprising the following raw materials in parts by weight: 12 parts of marmoset extract, 10 parts of Daphne chamaejasme extract, 8 parts of horseradish extract, 3 parts of Tinglizi oil, and 0.3 part of shikimic acid;

[0023] The extract of the marmoset is to remove impurities from the rhizome of the fresh marmoset, crush it and pass through a 60-mesh sieve, and place it in a solvent composed of water, ethanol, methanol, and acetic acid with a mass ratio of 100:20:8:8. Extracting three times at a temperature of 60° C. to obtain a supernatant, and filtering to obtain an extract of the rhizome of the marmoset;

[0024] The chamaejasma chamaejasme extract is soaked in distilled water twice the mass of the dried root of chamaejasma samanthus for 30 minutes, taken out, boiled on high heat, then decocted on low heat for 40 minutes, filtered, repeated water extraction twice, and combined three times of filtrate , concentrated ...

Embodiment 2

[0027] A bactericidal preparation of Morinda officinalis: comprising the following raw materials in parts by weight: 30 parts of marmoset extract, 25 parts of Daphne chamaejasme extract, 15 parts of wasabi extract, 5 parts of Tinglizi oil, and 1.5 parts of shikimic acid;

[0028] The extract of the marmoset is to remove impurities from the rhizome of the fresh marmoset, crush it and pass through a 100-mesh sieve, and place it in a solvent composed of water, ethanol, methanol, and acetic acid with a mass ratio of 100:20:8:8. Extracting three times at a temperature of 90° C. to obtain a supernatant, and filtering to obtain an extract of the rhizome of the marmoset;

[0029] The chamaema chamaejasme extract is prepared by adding the dry root of chamaejasma chamaejasme into 4 times the mass of distilled water and soaking for 60 minutes, taking it out, boiling it on high heat, then decocting it on low heat for 60 minutes, filtering, repeating the water extraction twice, combining th...

Embodiment 3

[0032] A bactericidal preparation of Morinda officinalis: comprising the following raw materials in parts by weight: 16 parts of marmoset extract, 18 parts of Daphne chamaejasme extract, 11 parts of horseradish extract, 4 parts of Tinglizi oil, and 0.9 part of shikimic acid;

[0033] The extract of the marmoset marmoset is to remove impurities from the fresh rhizome of marmoset marmoset, pass through an 80-mesh sieve after being crushed, and place it in a solvent composed of water, ethanol, methanol, and acetic acid with a mass ratio of 100:20:8:8. Extracting three times at a temperature of 70° C. to obtain a supernatant, and filtering to obtain an extract of the rhizome of the marmoset;

[0034] The chamaejasma chamaejasma extract is soaked in distilled water with 3 times the quality of the dried root of chamaejasma chamaejasma for 50 minutes, taken out, boiled on high heat, then decocted on low heat for 50 minutes, filtered, repeated water extraction twice, combined filtrate ...

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Abstract

The invention provides a morinda citrifolia sterilization preparation and a preparation method thereof. The sterilization preparation is prepared from, by weight, 12-30 parts of adenophora trachelioides extracting solution, 10-25 parts of stellera chamaejasme extract, 8-15 parts of Eutrema yunnanense extract, 3-5 parts of semen lepidii oil and 0.3-1.5 parts of shikimic acid. All the raw materials are matched to achieve the antibacterial effect in a synergistic mode, and the sterilization preparation has high sterilization activity. The sterilization effects especially on pythium, anthracis, fusarium and Capnodiales fungus which all reach 90% or above, and also has high prevention and treatment effects on morinda citrifolia stem rot and anthracnose caused by the fungi, the prevention and treatment effects respectively reach 86.2% and 84.2% after 14 days, and the prevention and treatment effects on anthracnose reach 85.7% and 84.5% after 14 days.

Description

technical field [0001] The invention relates to the field of agricultural sterilization, in particular to a Morinda officinalis bactericidal preparation and a preparation method thereof. Background technique [0002] Seaside wood Morinda citrifolia (scientific name: Morinda citrifolia L.), also known as Morinda citrifolia L., is a shrub to small tree of Rubiaceae and Morinda citrifolia, up to 5 meters high; the stem is straight, and the branches are nearly quadrangular. Leaves alternately opposite, both ends acuminate or acute, glossy, glabrous, veins protruding on both sides, axils of lower veins densely covered with short bundles of hairs; stipules between petioles, glabrous. Inflorescence head every other node, opposite to leaves, many flowers, sessile; calyx tubes are more or less glued to each other, calyx eaves nearly truncated; corolla white, funnel-shaped, lobes ovate-lanceolate, bearing corolla Throat, filaments about 3 mm long, anthers introverted, style about as ...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01N37/36A01P3/00
CPCA01N65/08A01N37/36A01N65/00Y02A50/30
Inventor 李小宝张斌孙龙雨范娜王妹
Owner HAINAN NORMAL UNIVERSITY
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