42 results about "Pyrethrin I" patented technology

The invention belongs to the technical field of pesticide residue detection, and particularly relates to a hydrophobic deep-eutectic solvent and application thereof in pyrethroid pesticide extraction. The hydrophobic deep-eutectic solvent provided by the invention comprises thymol serving as a hydrogen bond acceptor and coumarin or menthol serving as a hydrogen bond donor. Through the hydrophobic interaction between the hydrophobic deep-eutectic solvent and the hydrophobic pyrethroid pesticide, and the strong pi-pi action between the benzene ring structures of coumarin and thymol and the benzene ring of the pyrethroid pesticide, or the adsorption action between the aliphatic ring of menthol and the benzene ring of the pesticide, the pyrethroid pesticide can be prepared. The selective efficient enrichment of the pyrethroid pesticide can be realized through the combined action of the hydrophobic deep-eutectic solvent and the hydrophobic deep-eutectic solvent, the extraction efficiency of the pyrethroid pesticide by the hydrophobic deep-eutectic solvent can be greatly improved, and the detection sensitivity and the detection accuracy of the pyrethroid pesticide can be further improved.

The invention relates to a process for producing three recombinases capable of hydrolyzing organophosphorus, carbamate and pyrethroid insecticides, and preparations containing these enzymes. The present invention provides recombinant plasmid DNA vectors in which the protein secretion system has been modified to improve the ability of recombinant proteins to secrete into the space around the plasmid or culture medium. A nucleotide sequence for coding a PelB (butyric acid lyase B) signal sequence is deleted and substituted by a nucleotide sequence for coding a LamB (maltodextrin or phage lambda receptor) signal sequence, so that the secretion efficiency of the recombinant protein is higher. The invention discloses three recombinant plasmid DNA (deoxyribonucleic acid) vectors which are constructed by utilizing the improved plasmid DNA vectors and are used for expressing recombinant proteins. The recombinant protein comprises (a) a recombinant organophosphate hydrolase having at least 80% sequence identity with the amino acid sequence of methyl parathion hydrolase (MPH) (Protein Database (PDB) ID: 1P9E) as shown in SEQ ID NO: 1, wherein the recombinant organophosphate hydrolase has hydrolytic activity on organophosphate insecticides; (b) a recombinant carbamate hydrolase having at least 80% sequence identity with the amino acid sequence of L-aminopeptidase-D-Ala-esterase / amidase (aryl amidase) (MAH) (PDB ID: 1B65), as shown in SEQ ID NO: 2, wherein the recombinant carbamate hydrolase has hydrolytic activity on carbamate insecticides; and (c) a recombinant pyrethroid hydrolase having at least 80% sequence identity with the amino acid sequence of the pyrethroid hydrolytic carboxylesterase (EstA) (PDB ID: 4CCY) as shown in SEQ ID NO: 3, wherein the recombinant pyrethroid hydrolase has hydrolytic activity on a pyrethroid insecticide. The embodiment of the invention further provides three recombinant bacterial strains, an escherichia coli strain BL21 (DE3) is used as a host cell, and each recombinant plasmid DNA vector is used for transformation. The invention also discloses culture conditions of the recombinant strain, wherein the culture conditions comprise culture medium composition, induction conditions and enzyme harvesting and enzyme activity detection methods. In addition, the embodiment of the invention also provides a preparation which contains the three recombinases as functional components.

The invention belongs to the technical field of pesticide residue detection, and particularly relates to a magnetic fluid and application thereof in sample pretreatment and pyrethroid pesticide detection. The magnetic fluid provided by the invention comprises a hydrophobic eutecticevaporate solvent and magnetic nanoparticles dispersed in the hydrophobic eutecticevaporate solvent, wherein the hydrophobic eutecticevaporate solvent comprises coumarin serving as a hydrogen bond donor and thymol serving as a hydrogen bond acceptor. Through the hydrophobic interaction between the magnetic hydrophobic deep-eutectic solvent and the hydrophobic pyrethroid pesticide and the strong pi-pi action between benzene ring structures of coumarin and thymol and benzene rings of the pyrethroid pesticide, the extraction efficiency of the magnetic fluid on the pyrethroid pesticide is improved; the detection sensitivity and the detection accuracy of the pyrethroid pesticide can be improved; the magnetic nanoparticles are added, so that the magnetic fluid can realize phase separation by adopting an external magnetic field, the operation is simple and rapid, and the detection process of pyrethroid pesticide residues is simplified.

The invention discloses a plant-derived volatile mosquito repellent liquid for cats and a preparation method thereof. The mosquito repellent liquid comprises the following components in percentage by mass: 15-40% of plant essential oil, 0.02-0.2% of an antioxidant and 60-85% of a solvent. According to the cat mosquito repellent, the plant essential oil which is safe and harmless to cats and people is adopted to replace pyrethrin which is common in the market and serves as an effective mosquito repellent ingredient, the mosquito repellent effect is guaranteed, meanwhile, adverse effects on cat health are avoided, the plant essential oil is low in price and easy to obtain, the preparation method is simple, and the cat mosquito repellent is suitable for industrial production.

The invention relates to the technical field of compound preparation, in particular to chiral pyrethroid as well as a preparation method and application thereof. The structural general formula of the chiral pyrethroid is shown as a formula I or a formula II. The synthetic method has a wide substrate range, can successfully prepare a single configuration of a pyrethroid product and test the biological activity of the pyrethroid product, is simple and convenient to operate and high in yield, allows an optical pure isomer to reach 85% or more, prepares the pyrethroid product by a one-pot multi-step method, ensures the ee value and yield of the single configuration, and has a wide application prospect. The whole activity of the compound on plutella xylostella and spodoptera frugiperda is shown as 1R-cisgt; rac, cisgt; according to the present invention, the pyrethroid is a pyrethroid product, the 1S-cis is the low-efficiency body or the ineffective body of the pyrethroid product, and has wide application prospects in the academic field and the pesticide industry.

The present application relates to a multi-temperature zone mosquito prevention and control tablet, including a tablet substrate; a middle portion of the tablet substrate is provided with a first drug zone, and two sides of the tablet substrate are provided with a second drug zone; the first drug zone is filled with pyrethroid, and the second drug zone is filled with a repellent drug; and the repellent drug includes picaridin, dimethyl phthalate, methyl nonyl ketone, and diethylmethylbenzamide. The present application may diffuse different repellent drugs in combination, to achieve the composite mosquito prevention and control effect; and in addition, the present application only needs to heat the first drug zone and does not increase the energy consumption of an electric mosquito killer used in combination.

Provided is a composition for insect pest control containing natural pyrethrin, a radical chain inhibitor, a peroxide decomposer, and a solid carrier. The pest control composition has a ratio (V4 / V1) of a natural pyrethrin transpiration rate V4 (mg / hour) after heating at 180°C for 4 hours to a natural pyrethrin transpiration rate V1 (mg / hour) after heating at 180°C for 1 hour of 0.2-1.5.

The invention relates to a method for detecting multiple pesticide residues in tea leaves, which is suitable for quantitative analysis of organophosphorus, organochlorine and pyrethroid pesticides. The method comprises the following steps: adding water and acetonitrile into a tea sample, extracting to form a first extraction system, centrifuging, taking supernate, concentrating, and mixing with normal hexane and a saturated sodium chloride solution to form a second extraction system; and respectively taking an n-hexane layer, blow-drying the n-hexane layer, dissolving the n-hexane layer by using an elution solvent, purifying the n-hexane layer by using a solid-phase extraction column, fixing the volume, filtering the n-hexane layer, and detecting the n-hexane layer by using a gas chromatograph-tandem mass spectrometer. The method is high in sensitivity, good in linearity and suitable for various tea leaf samples; a PSA / Carb combined filler and an acetone-ethyl acetate elution system are adopted, so that the purification effect and the operation safety are improved, and the method is suitable for popularization and application.

A process for producing three recombinant enzymes capable of hydrolyzing each class of organophosphate, carbamate, and pyrethroid insecticides, as well as a formulation containing these enzymes provides the recombinant plasmid DNA vector, in which the protein secretory system has been modified to enhance the secretion of recombinant protein into the periplasmic space or culture medium. A nucleotide sequence encoding PelB (pectate lyase B) signal sequence for periplasmic localization is deleted and replaced with a nucleotide sequence encoding LamB (maltoporin or phage lambda receptor) signal sequence to enable a more efficient secretory recombinant protein. Three recombinant plasmid DNA vectors for the expression of recombinant proteins that are constructed using the modified plasmid DNA vector described above. The recombinant proteins include a recombinant organophosphate-hydrolyzing enzyme; a recombinant carbamate-hydrolyzing enzyme; and a recombinant pyrethroid-hydrolyzing enzyme.

The invention discloses a syringate compound capable of preventing and treating barn pests as well as a preparation method and application of the syringate compound. The syringate compound has the following molecular structure expression, and according to the design, the light stability is enhanced by utilizing a rigid cyclic structure of chrysanthemic acid, and the CarE activity can be inhibited through a propenyl side chain of eugenol, so that self metabolism inactivation of pyrethrin is blocked; .

The application discloses a CTAB modified magnetic waste fungus chaff biochar adsorbent and a preparation method and application thereof in pyrethroid detection. 3+ , Fe 2+ and the fungus chaff biochar, adding an alkaline reagent to carry out a coprecipitation reaction to obtain a magnetic biochar precursor; S3, pyrolyzing the magnetic biochar precursor to obtain a magnetic biochar; S4, adding the magnetic biochar into a cetyltrimethylammonium bromide solution to carry out ultrasonic treatment, and carrying out magnetic separation to obtain an adsorbent MBC-CTAB. The application establishes a pyrethroid analysis method in water samples by taking the MBC-CTAB as the adsorbent, is favorable for utilization of the waste fungus matrix, and adopts a magnet to separate the sample solution and the MBC-CTAB, replaces complicated separation equipment, and realizes convenient and rapid separation.

The invention relates to a quick detection method of pyrethroid, belongs to the technical field of fluorescent probes, and discloses a plurality of micromolecular fluorescent probes based on coumarin derivatives as well as a preparation method and application of the micromolecular fluorescent probes. The invention provides a plurality of micromolecular fluorescent probes DAC, DBC, DCC, DDC, DEC, DFC, DGC, DHC and DIC for targeted detection of pyrethroid pesticides based on coumarin derivatives, when the probes act with the pyrethroid pesticides, the fluorescence becomes strong from weak, and the fluorescence intensity is significantly enhanced in a certain range. Fluorescence performance tests show that the coumarin derivative-based micromolecular fluorescent probe pyrethroid pesticide has good linear response, and has good stability and strong anti-interference capability under different pH, temperature, salt concentration and illumination conditions. According to the actual sample detection principle, the pyrethroid pesticide in crops is detected, the detection limit is as low as 9.7 pM, the adding standard recovery rate is 99%-102%, and the results are consistent with those of a liquid chromatography method and an on-site rapid detection method. Therefore, the fluorescent probe provided by the invention has a good application prospect.

The invention discloses a method for preparing a long-acting electric mosquito-repellent incense sheet. By weight percentage, 50-80% of mosquito-repellent essential oil, 15-35% of a solubilizing agent and 5-15% of a ferrocene carboxylic acid derivative are uniformly mixed to obtain a mosquito-repellent enhancing liquid, and then pyrethroids are added and loaded on a carrier sheet to obtain the mosquito-repellent enhancing liquid. The electric mosquito-repellent incense sheet has a sustained release property, can uniformly release mosquito-killing components over a long period of time, has a relatively stable volatilization amount, and has a long-acting mosquito-killing effect.

The invention discloses an insecticidal composition containing cyprosulfuron and application of the insecticidal composition. The effective components of the insecticidal composition are composed of cyprosulfuron and pyrethroid, wherein the pyrethroid is perfluthrin, dimefluthrin, trifluthrin, dextro-trans-propargyl chloride pyrethrin, perfluthrin, dextro-phenothrin, dextro-tefluthrin, chlorenthrin, beta-cypermethrin, meperfluthrin, bifenthrin, deltamethrin, cis-cypermethrin and beta-cyhalothrin, and the pyrethroid is a mixture of dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin, dimefluthrin and dimefluthrin. Any one of beta-cyfluthrin and beta-cyfluthrin; the total weight of the cyprosulfuron and the pyrethroid accounts for 2.2%-40% of the insecticidal composition, and the weight ratio of the cyprosulfuron to the pyrethroid is (10: 1)-(1: 2). The pesticide has the characteristics of high efficiency, low toxicity and the like, is used for space spraying or retention spraying, is quick in effect, thorough in killing and long in lasting period, reduces the pesticide application amount, and reduces the pesticide application irritation. The composition can be widely applied to hotels, schools, factories, farms, families and public places, and has a special effect on preventing and treating sanitary pests.

The invention discloses a preparation method of sodium alginate mediated MIP-Fe3O4-NCs immobilized bacteria for adsorbing and degrading a trace amount of pyrethroid pesticides, which is characterized by comprising the following steps: preparing mesoporous molecular imprinting modified Fe3O4-NCs by a surface grafting method to obtain surface imprinting functionalized Fe3O4 nanostructure magnetic microspheres MIP-Fe3O4-NCs; the preparation method comprises the following steps: mixing MIP-Fe3O4-NCs activated by methanol with a sterilized sodium alginate solution, then adding a pyrethroid pesticide degrading bacterium solution, fully and uniformly stirring, then adding into cyclohexane, uniformly stirring again, dropwise adding a calcium chloride solution, and carrying out a cross-linking reaction at 4 DEG C for 2-6 hours to obtain the MIP-Fe3O4-NCs immobilized bacterium. The intercellular mass transfer resistance is reduced, the sensitivity of microorganisms to ultra-trace pyrethroid pesticides in the environment is greatly improved, and the degradation efficiency is improved.

The invention provides a method for determining pesticide residues in medicinal and edible substances through homogeneous liquid-liquid microextraction, which is characterized in that green, rapid and efficient homogeneous liquid-liquid microextraction is carried out on pyrethroid pesticides in the medicinal and edible substances, and 2-methyltetrahydrofuran is used as an extracting agent; the method comprises the following steps: dissolving an extraction agent in a sample solution to make the extraction agent fully contact with the pyrethroid pesticide, and adding ammonium sulfate to separate the extraction agent from the sample solution so as to complete the extraction of the pyrethroid pesticide. The whole extraction process takes less than 1 minute, and no extra extraction or centrifugal equipment is needed. And carrying out quantitative analysis by adopting a high performance liquid chromatography. The method has the advantages of being environmentally friendly, rapid in operation, high in efficiency and the like, the bio-based solvent is introduced to serve as a substitute of a toxic solvent acetonitrile, the limitation of extraction agent selection in homogeneous phase liquid-liquid microextraction is solved, the homogeneous phase liquid-liquid microextraction method with more excellent performance is developed, and the method is suitable for industrial production. The method has application potential in trace analysis of pesticides in various medicinal and edible substances.

The present application relates to a kind of pyrethroid pesticides solid phase microextraction adsorbent, it is characterized by 2,3,5,6-tetrafluoro-p-phenylenediamine and 2,4,6-trifluoro-1,3,5-triacetyl benzene as reactant monomer by condensation reaction obtained polymer.Its preparation process includes: solvent is put into reaction kettle, under continuous stirring, add reactant monomer mixture and catalyst, normal temperature reaction 10-15 hours, filter out solid, respectively with solvent and methanol wash after vacuum drying, obtain cluster polymer.The polymer has good adsorption selectivity to pyrethroid pesticides, can be used as the solid phase microextraction adsorbent of pyrethroid pesticides, for making solid phase microextraction probe.

A composition comprising natural pyrethrin, one or more phenolic antioxidants selected from Group A, and a solid carrier;　wherein Group A is a group consisting of 31 specific compounds having a phenolic structure, such as 2,2'-methylenebis(6-tert-butyl-4-methylphenol), 3-hydroxytyrosol, or carnosic acid.

The invention belongs to the technical field of insecticide byproducts, and provides a byproduct recycling method of a refined pyrethrin product. The recycling method comprises the following steps: (1) collecting by-products; (2) detoxification treatment: performing high-temperature steam treatment on the disintegrating slag, and cooling to obtain pretreated disintegrating slag; mixing the pretreated disintegrating slag with an ethanol water solution, and performing ultrasonic extraction; after the extraction is finished, carrying out solid-liquid centrifugal separation to obtain primarily detoxified residues; mixing the preliminarily detoxified residues with a solution of a compound enzyme, performing enzymolysis, and then filtering to obtain deeply detoxified residues; (3) drying treatment: drying the deeply detoxified residues to obtain pretreated residues; and (4) preparing biomass particles: adding auxiliary materials into the pretreated residues, granulating, cooling and screening to obtain the biomass particles. According to the recycling method, biomass components in the solid residues generated after fine extraction of pyrethrin can be converted into biomass energy, the problem of byproduct treatment can be solved, environmental pollution can be reduced, resource recycling can be achieved, and economic value can be created.

The present invention pertains to a composition or the like containing a natural pyrethrin and an antioxidant selected from group P or group S.　Group P: A group comprising compounds represented by formula (1) [R1 and R2 are the same or different from each other and each represent a C9-C14 alkyl group], 3,9-bis(2,4-di-tert-butylphenoxy)-2,4,8,10-tetraoxa-3,9-diphosphaspiro[5.5]undecane, tris(nonylphenyl) phosphite, triphenyl phosphite, tetrakis(2,4-di-tert-butyl phenyl) 4,4'-biphenylene diphosphinite, tridodecyl trithiophosphite, etc. Group S: A group comprising pentaerythritol tetrakis[3-(dodecylthio)propionate], 2-mercaptobenzimidazole, 2,4-bis[(dodecylthio)methyl]-6-methylphenol, and didodecyl 3,3'-thiodipropionate.

The present invention relates to a composition containing natural pyrethrin and a phosphorus-based antioxidant selected from the group P. Group P: tetraphenyldipropylene glycol diphosphite, diisodecylphenylphosphite, diphenyl mono (2-ethylhexyl) phosphite, di-n-decylphenylphosphite, diphenylmono (tridecyl)phosphite, 3,9-bis(nonylphenoxy)-2,4,8,10-tetraoxa-3,9-diphosphaspiro[5.5]undecane, 2,2'- methylenebis(4,6-di-tert-butylphenyl) 2-ethylhexylphosphite, hydrogenated bisphenol A pentaerythritol phosphite polymer, distearyl pentaerythritol diphosphite, tris(2,4-di-tert-butylphenyl) phosphite, and tris(tridecyl) phosphite.

The present invention relates to topical pharmaceutical compositions comprising a corticosteroid and an insecticide selected from pyrethrin or a synthetic pyrethroid insecticide. Methods for their use in the treatment of allergic dermatitis, particularly hypersensitivity to insect bites, in animals using the compositions of the invention are also described.

This invention discloses a bamboo charcoal fiber cleaning cloth with antibacterial and antiviral functions and its preparation method, belonging to the field of functional fiber materials technology. The cleaning cloth uses bamboo charcoal fiber as the main body, combined with phosphorylated modified mulberry bark fiber. Zinc ions are used to chelate tea polyphenols and pomegranate peel tannins to form active components, which are then encapsulated in sodium alginate microcapsules and loaded into the porous structure of the fibers. Natural pyrethroid extract and β-cyclodextrin are added to achieve mite prevention and odor suppression. The preparation employs a green process of ultrasonic-assisted chelation and high-voltage electrostatic spray microcapsule preparation. This invention solves the problems of easy loss of antibacterial components and poor mechanical properties in traditional cleaning cloths. After 200 washes, the antibacterial rate remains at 95%-99%, and the tensile strength is 13.5-16.0 CN / dtex. It also has the advantages of being all-natural, residue-free, environmentally friendly, and mass-producible, making it suitable for household and medical cleaning applications.

The present disclosure provides a 3-phenoxybenzoic acid-glucuronic acid conjugate, and a preparation method and use thereof, and belongs to the technical field of pesticide detection. Compared with 3-phenoxybenzoic acid, the 3-phenoxybenzoic acid-glucuronic acid conjugate provided by the present disclosure features structural stability, high specificity, long limit of detection, and high content in urine, and can better serve as a marker that identifies whether an organism is killed due to pyrethroid pesticide poisoning. Namely, the 3-phenoxybenzoic acid-glucuronic acid conjugate can detect whether a toxicant (pyrethroid pesticides) is taken antemortem or exposed postmortem, and has an excellent application prospect in pyrethroid pesticide detection. The present disclosure provides a preparation method of a 3-phenoxybenzoic acid-glucuronic acid conjugate. The preparation method provided by the present disclosure features high product yield, simple operation, wide raw material sources, low costs, and suitability for industrial production.

The present invention relates to a phytosanitary product comprising: (a) 5-85% an active substance selected from paraffin oil, a C7-C20 fatty acid or salts thereof, maltodextrin, pyrethrin, spinosad, azadirachtin, Bacillus thuringiensis, Beauveria bassiana, Cydia pomonella granulovirus, Allium sativum extract, citronella oil, clove oil, rapeseed oil, spearmint oil, orange oil, limonene oil, sunflower oil or onion oil, tea tree extract, eugenol, geraniol, thymol, deltamethrin, lambda cyhalothrin, lecithin, Salix spp. bark, Urtica spp. extract, L-cysteine, Equisetum arvense L., Allium cepa L. extract, Helicoverpa armigera nucleopolyhedrovirus (HearNPV), Quassia amara L. extract, Reynoutria sachalinensis extract, giant knotweed extract; Fallopia sachalinensis; Polygonum sachalinense; white willow bark extract, Yucca schidigera extract and sweet Lupinus albus extract; (b) 5-60% non-ionic surfactant; and (c) 5-80% anionic surfactant.

To provide a bug-proof chemicals volatilization tool that improves a repellent effect, an extermination effect or an attraction effect against bugs such as ticks and clothing bugs.SOLUTION: A bug-proof chemicals volatilization tool 1 comprises a cotton sheet that is impregnated with either fatty acids with 6-18 carbons or at least one kind selected from among fatty acid ester, a pyrethroid compound and an essential oil. Characteristically, the basis weight of the cotton sheet is 10-250 g / m2.SELECTED DRAWING: Figure 1

The present invention relates to a composition containing a synthetic pyrethroid compound and formula (1). (In formula (1), R1 and R2 may be the same as or different from each other and each represents a C9-C14 alkyl group. )

The invention belongs to the field of agricultural microbial technology and agricultural product safety, and particularly relates to application of a method for preparing a functional biological flora in a targeted biological flora and host original flora coupling mode in rice cultivation. According to the method, bacillus subtilis, bacillus mucilaginosus, brevibacillus laterosporus and host original flora are subjected to coupled symbiotic culture to form specific functional flora, and the specific functional flora is compounded with nutrient carriers such as humic acid potassium fulvate and amino acid chelated trace elements to prepare the targeted biological bacterium water-soluble fertilizer. The microbial agent effectively reduces the bioavailability of heavy metals such as cadmium, lead and arsenic in soil and migration of the heavy metals to rice; meanwhile, the functional flora secretes hydrolase, oxidoreductase and the like, degrades pesticide residues such as organophosphorus and pyrethroid and decomposes the pesticide residues into non-toxic or low-toxicity substances, the method is specially used for solving the problem that heavy metal and pesticide residues in the rice in the filling stage of the rice exceed the standard, and the quality and edible safety of the rice are guaranteed from the source.

The present invention relates to a composition and to the production and use of said composition.Pesticide synergist composition containing, among others, (1) a cysteine proteolytic enzyme (or a mixture of cysteine proteolytic enzymes), (2) a polyphenolic compound (or a mixture of polyphenolic compounds chosen from among hydrolysable tannins), (3) a compound chosen from among the triglycerides (or a mixture thereof), (4) a phase change inhibitor chosen from among (ethanol, isopropyl alcohol, benzylic alcohol and the mixtures thereof), (5) an enzyme stabilizer chosen from among the organic peroxides, (6) a non-ionic surfactant, (7) an essential oil chosen from among the essential oils of Melaleuca alternifolia and quinquenervia, (9) a simple reagent such as acetic acid and citric acid to modify / stabilize the pH the present invention may be in the form of a concentrate or an emulsion containing pyrethrins (pyrethrum flower extract), pyrethroids (i) and (ii), and a mixture thereof. said composition is intended for use in managing the resistance associated with the mechanisms of insects for detoxifying insecticides and to improve the efficiency of certain insecticides, in particular certain insecticides such as pyrethrins and pyrethroids (i) and (ii).

The present invention relates to a composition which contains a synthetic pyrethroid compound and a compound represented by formula (1) (wherein R1 and R2 are the same or different and each represent a C17-C19 alkyl group), wherein the mass ratio of the synthetic pyrethroid compound to the compound represented by formula (1) is 1:0.01 to 1:20.