58 results about "Pythium" patented technology

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention belongs to the technical field of gene engineering, and relates to application of a potato late blight bacterium response gene StRTP5 in regulation and control of potato disease resistance. The invention provides application of a potato late blight response gene StRTP5 in regulation and control of potato disease resistance, the potato late blight response gene StRTP5 comprises a gene StRTP5a and a gene StRTP5b, the gene StRTP5a and the gene StRTP5b negatively regulate and control resistance of potatoes to phytophthora infestans and alternaria solani, and the gene StRTP5a and the gene StRTP5b have potential to be used for creating potato broad-spectrum disease-resistant materials.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention discloses a compound microbial agent for preventing and treating muskmelon fusarium wilt and application of the compound microbial agent, and belongs to the field of biological prevention and treatment. The microbial inoculum is composed of pseudomonas aeruginosa and bacillus subtilis, the dosage form is a liquid microbial inoculum, and the effective viable count is greater than 10 billion / ml. The two strains are high in sporulation quantity and strong in rhizosphere colonization capacity, have synergistic bacteriostasis and growth promotion effects, effectively inhibit fusarium oxysporum of muskmelons through a plurality of mechanisms such as competition, secondary metabolite generation and system resistance induction, and have a remarkable inhibition effect on fusarium oxysporum of cucumbers, watermelons and tomatoes, soil-borne pathogenic bacteria such as phytophthora capsici and the like. The microbial agent is broad-spectrum, efficient and long in lasting period, and can promote propagation of rhizosphere effective microbial communities, reduce the dosage of chemical pesticides, relieve continuous cropping obstacles and promote green and high-quality development of the watermelon and melon industry.

The invention discloses a microbiological degraded Betallop simulation sample and a preparation method thereof, and belongs to the technical field of cultural relic degradation simulation. The method comprises the following steps: inoculating a specific decay-causing fungus suspension on the surface of a bay leaf simulated substrate for microbial degradation, wherein the specific decay-causing fungi comprise penicillium chrysogenum and humicola brownish black. The penicillium chrysogenum and the humicola brownish black obtained through screening have important values for researching a biological degradation mechanism of the cultural relics and developing a targeted biological prevention and control technology, and the simulation sample prepared by the method provides a standardized sample for protecting and repairing the Betashes; the method can be used for safely and efficiently verifying various reinforcing materials, mildew preventive, cleaning agent, protection process and repair technology and researching the aging mechanism, greatly reduces the risk of directly testing on precious cultural relics, and has important significance on promoting the scientific protection of plant fiber cultural relics such as Beijing.

The invention provides an alkaloid Waltherione S analogue as well as a synthesis method and application thereof, and relates to the technical field of alkaloid Waltherione compounds. According to the present invention, the structures of the Waltherione A and the Antidesmone are simplified, the pyridine and pyridone alkaloid Waltherione S analogue is designed and synthesized, and the synthesized compound has broad-spectrum bacterial killing activity on banana wilt pathogen, wheat scab pathogen, banana anthracnose pathogen, phytophthora capsici pathogen, botrytis cinerea, rhizoctonia solani, rice blast pathogen, mango stem-end rot pathogen and the like, the compound also has good nematicidal activity on root-knot nematodes, provides a favorable candidate agent for natural, safe and low-toxicity innovative pesticides, and is expected to be applied to agricultural production.

The application discloses a cyclohexane-fused masked 2-pyridone compound and a preparation method and application thereof, and relates to the technical field of organic synthesis. The preparation method comprises the following steps: taking a simple and easily obtained substituted cyclohexane cyclopentadienyl ester as raw material, taking iodobenzene diacetic acid as an oxidant, taking ammonium carbamate as a nitrogen source, taking magnesium oxide and water as additives, taking chloroform and 2,2,2-trifluoroethanol as mixed solvents, and reacting at room temperature to obtain the cyclohexane-fused masked 2-pyridone compound. The application has the advantages of mild reaction condition, simple operation, high yield, wide reaction substrate range and the like, and does not need to use a metal catalyst, does not need to be heated and does not need to be operated in a water-free and oxygen-free manner. The cyclohexane-fused masked 2-pyridone compound prepared by the application has a good inhibiting effect on plant pathogenic fungi, and can be used for the prevention and treatment of tomato gray mold, cereal scab, rapeseed sclerotinia, pepper pythium blight and grape cavity disease.

A system and methods for gas infusion tailored for manufacturing, prevention, and the application of fungicides in agriculture and aquaculture includes a microporous hollow fiber gas infusion module, engineered to efficiently dissolve gases such as oxygen or ozone into aqueous streams, thereby revolutionizing disease prevention and treatment strategies. Complementing the gas infusion module is a suite of manufacturing equipment and methodologies tailored for the production of oxygen-infused or other nutrient gas-infused products, catering to the diverse needs of agricultural and aquacultural industries. The system and method addresses the pervasive challenges posed by fungal pathogens in agriculture, such as Botrytis cinerea, Sclerotinia sclerotiorum, Pythium spp., and in aquaculture, particularly Saprolegnia, by offering comprehensive solutions encompassing precise gas infusion techniques, innovative disease prevention strategies, and sustainable manufacturing processes to enhance crop yields, promote environmental sustainability, and ensure the long-term viability of agricultural and aquacultural sectors.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

This invention relates to a microbial composition for preventing and controlling root rot in tea trees and its application, belonging to the field of microbial and organic fertilizer technology. The composition comprises the following components: Component 1, Bacillus kaempferi and Trichoderma yunnanensis; Component 2, humic acid, Chrysanthemum indicum extract, amino acids, brown algae oligosaccharides, complex vitamins, and trace elements; wherein, Bacillus kaempferi is N4471 (…). Bacillus cabrialesii The accession number is CCTCC NO: M20221660, and the Yunnan Trichoderma is YNK-FG0001 ( Trichoderma yunnanense The microbial composition, with accession number CCTCC NO: M 2025645, significantly improved soil water stability aggregates, pH, and cation exchange capacity when applied to tea plant cultivation. It also enhanced root vitality and the activity of antioxidant enzymes [superoxide dismutase (SOD) and peroxidase (POD)], thereby reducing the growth of Fusarium rot (…). Fusarium solani ), Pythium ( Pythium spinosum ) and teosinte ( Rosellinia arched The disease index of tea tree root rot caused by infection was used to ultimately achieve effective control of tea tree root rot.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

The application discloses a primer composition for detecting Pythium sylvesterianum based on LAMP technology and application thereof, and belongs to the technical field of microorganism detection; the primer composition is a LAMP primer composition designed according to a specific sequence shown in SEQ ID NO. 1. The application adopts a method for rapidly detecting Pythium sylvesterianum, the pathogenic bacterium of stem base rot of raw ginger, based on loop-mediated isothermal amplification technology, and establishes a perfect detection and field real-time detection system for soil samples, water source samples and diseased plant samples, thereby opening up a new train of thought and method for the measurement and prediction of field crop diseases, winning valuable time for the prevention and control of related diseases, and reducing the loss caused by diseases to a certain extent.

The utility model relates to the technical field of sampling devices, in particular to a sampling device for detecting boea bombycis, which comprises a fixing plate, grooves are arranged at the front end and the rear end of the fixing plate, guide rods are fixedly connected to the inner sides of the grooves, and a second reset spring is sleeved at one end of the outer surface of each guide rod. And one end of the second reset spring is fixedly connected with a moving block sliding on the outer surface of the guide rod, the left side and the right side of the moving block are fixedly connected with a connecting block and a handle correspondingly, the left side of the connecting block is fixedly connected with a fixing sleeve, and one side of an inner cavity of the fixing sleeve is fixedly connected with a first reset spring. Under the action of the moving block, the handle and the second reset spring, the sampling device has the advantages of simplicity in operation, small size and convenience in carrying, and solves the problems that an existing sampling device for detecting the boea bombycis disease is relatively complicated to operate and use, is relatively large in size and is inconvenient to carry, and the use of detection personnel is influenced.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.

The application discloses a specific detection target Pc_s103050 of a pathogenic bacterium of cedar root rot and application, and the specific detection target comprises a nucleotide sequence as shown in SEQ ID NO. 13, i.e. Pc_s103050. The LAMP primer composition designed based on the target gene disclosed by the application can be used for rapidly and efficiently detecting Pythium cedri, and the detection method has the advantages of high specificity, high accuracy, good sensitivity, simple operation and naked-eye observation of results, and Pythium cedri can be identified when the DNA reaches 100 pg mu L ‑1 .

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The present application belongs to the technical field of tobacco planting, and particularly relates to a method for targeted removal of pathogenic bacteria in continuous tobacco field, which comprises the following steps: S1, land preparation: removing field residues and weeds before transplanting flue-cured tobacco seedlings; S2, pesticide application: applying base fertilizer and removal agent to the tobacco ridge area of the tobacco field; S3, ridging: loosening and ridging the soil where the removal agent is applied; S4, film mulching and sealing: mulching after ridging, and sealing the tobacco ridge; S5, film breaking and air release: breaking the film at the interval of the tobacco seedling nest distance and releasing the air in the film; S6, trial planting and verification: planting 3-5 tobacco seedlings or vegetable seedlings per mu, watering and observing, and then transplanting after no abnormality of the plants; S7, transplanting tobacco seedlings; in use, the inhibition rates of Fusarium, Pseudomonas and Pythium in the continuous tobacco field are all above 86%, the comprehensive removal rate of pathogenic bacteria is greater than or equal to 86%, the incidence of tobacco seedlings is reduced from 36.8% to less than 7%, the frequent occurrence of soil-borne diseases is solved from the source, the bacteria are targetedly inhibited, no fallow is needed, and the cost is low.

The application relates to a cultivating method of salt-tolerant bacteria and agricultural application, and the cultivating method comprises the following steps: S1, mixing straw, mushroom cultivation waste, poultry manure, inorganic salt and culture medium matrix to obtain a biological waste-based culture medium; S2, respectively culturing pythium and bacillus by using the biological waste-based culture medium to obtain a compound bacterial strain liquid; S3, respectively mixing diatomite and the compound bacterial strain liquid, adsorbing and drying to obtain an intermediate product; S4, blending chitosan, phytanic acid and an activator, and reacting to obtain hydrophobic chitosan; and S5, blending and stirring the intermediate product and the hydrophobic chitosan to obtain salt-tolerant bacteria. The hydrophobic chitosan is beneficial to stable storage of the salt-tolerant bacteria in a high-humidity environment, reduces adverse effects such as growth inhibition between different salt-tolerant bacteria, and makes different salt-tolerant bacteria play a synergistic role, so that the efficiency of the fertilizer is finally improved.

The invention relates to pseudomonas fluorescens JL12 with the effect of preventing and treating tobacco black shank and application of the pseudomonas fluorescens JL12. The pseudomonas fluorescens JL12 is classified and named as pseudomonas fluorescens, and the preservation number of the pseudomonas fluorescens JL12 is CGMCC No. 34713. The pseudomonas fluorescens JL12 strain can effectively antagonize phytophthora, prevent the incidence of tobacco black shank and weaken related symptoms of the tobacco black shank, so that the pseudomonas fluorescens JL12 strain can be used for preparing products for preventing and treating the tobacco black shank and improving the tobacco cultivation quality and yield.

The invention discloses an antibacterial compound derived from bacillus velezensis and application of the antibacterial compound, and belongs to the technical field of agricultural microbial biocontrol. The compound with the activity of inhibiting fusarium and pythium pathogenic fungi is identified from bacillus velezensis metabolites, and the compound has a good application prospect in the aspect of developing and preventing agents. The biocontrol nutrient solution formed by the antibacterial compound provided by the invention can inhibit pathogenic fungi of fusarium and pythium at the same time, is practically applied to a field for planting potatoes, and improves the yield and quality of the potatoes by reducing the incidence rate of crops.

The invention relates to a microbial composition for preventing and treating root rot of tea trees and application thereof, and belongs to the technical field of microorganisms and organic fertilizers, the composition comprises the following components: component 1, bacillus caramei and trichoderma yunnanense; component 2: humic acid, crowndaisy chrysanthemum extract, amino acid, alginate oligosaccharide, multivitamins and medium trace elements; wherein the bacillus caramei is N4471, the preservation number of the bacillus caramei is CCTCC (China Center For Type Culture Collection) NO: M20221660, the trichoderma yunnanense is YNK-FG0001 (Trichoderma yunnanense), and the preservation number of the trichoderma yunnanense is CCTCC NO: M 2025645. When the microbial composition is applied to tea tree planting, soil water-stable macroaggregate, pH and cation exchange capacity can be remarkably improved, root activity and antioxidant enzyme [superoxide dismutase (SOD) and peroxidase (POD)] activity are improved, the disease index of tea tree root rot caused by infection of Fusarium solani, Pythium spp and Rosellinia arcuata is further reduced, and the disease resistance of the tea tree root rot is improved. And finally, effective prevention and control of the tea tree root rot are realized.

The application provides a kind of heterocyclic imidazole-2-oxazoline compound and its preparation method and application, belong to chemical synthesis technical field.The heterocyclic imidazole-2-oxazoline compound provided by the application has the structure shown in formula A, which includes heterocyclic and oxazoline fragment in structure, has certain inhibitory effect on various plant pathogenic fungi (such as rice sheath blight fungus, oilseed rape sclerotinia, tomato botrytis cinerea, wheat scab fungus, pepper pythium disease fungus), is expected to be used as a new type of fungicide candidate compound or directly as fungicide, at the same time, heterocyclic imidazole-2-oxazoline skeleton as ligand molecule with chemical catalytic activity, has important significance for biological and chemical research field.The preparation method of the above compound is provided, which is synthesized by using simple and easy-to-synthesize heterocyclic imidazole-2-carboxylic acid as starting material, the synthesis route is short, the raw material is easy to obtain, and industrialized batch production is easy to realize.

The invention discloses streptomyces murinus FLWZ-0001 and application of the streptomyces murinus FLWZ-0001 in prevention and treatment of fungal and oomycete diseases of crops. The streptomyces murinus FLWZ-0001 is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the streptomyces murinus FLWZ-0001 is CCTCC (China Center for Type Culture Collection) NO: M20241923. The strain and the fermentation liquor thereof have a remarkable inhibition effect on various plant pathogenic bacteria, can efficiently inhibit fungal pathogenic bacteria such as sclerotinia sclerotiorum, botrytis cinerea and penicillium digitatum, and can also efficiently inhibit phytophthora capsici and phytophthora nicotianae belonging to oomycetes. Pot experiments show that fermentation liquor of the strain has excellent prevention and treatment effects on multiple crop diseases such as sclerotinia rot of colza, black shank of colza, phytophthora blight of pepper, gray mold and the like. The invention provides a brand new microbial resource for developing a broad-spectrum, efficient and environment-friendly multi-target biopesticide, and has a wide application prospect.

The application belongs to the technical field of plant disease resistance breeding, and provides application of GmbZIP111 protein and / or its coding gene in improving resistance of soybean to pythium. GmbZIP111 The gene positively regulates resistance of soybean to pythium root rot, overexpression of the gene can significantly inhibit pythium infection of soybean, and knockout of the gene leads to reduced resistance. GmbZIP111 The gene has important theoretical significance and application value for analyzing the mechanism of soybean resistance to pythium root rot and cultivating new disease-resistant varieties.

The invention discloses a chimeric immune receptor and application of the chimeric immune receptor to improvement of phytophthora resistance of plants, and belongs to the technical field of plant disease control. The chimeric immune receptor disclosed by the invention can specifically recognize the phytophthora sojae effector Avh85 or a homolog thereof, and can be activated only when a specific pathogenic bacterium effector exists, so that accurate recognition of target pathogenic bacteria is realized, and unnecessary energy consumption of a plant immune system is avoided. Once the chimeric immune receptor disclosed by the invention is activated, multiple and potent immunoreactions including significant up-regulation of defensive genes, active oxygen outbreak and MAPK phosphorylation can be initiated, a firm defensive barrier is formed together, and expansion of pathogenic bacteria can be rapidly and efficiently suppressed.

This invention discloses an endophytic dispersible pantothecin strain X24004 from maize and its applications. The dispersible pantothecin strain of this invention (… Pant disperse X24004, with its preservation number CGMCC No. 34510, exhibits strong antagonistic effects against various pathogenic fungi, including *Fusarium graminearum* (causal agent of corn stem rot), *Fusarium verticillatum* (causal agent of corn ear rot), and *Pythium styracifolium* (causal agent of corn stem rot). It demonstrates high efficiency and broad-spectrum control of plant fungal diseases while also promoting corn plant growth. It is a broad-spectrum biological agent, bio-fertilizer, and seed treatment agent with promising development and application prospects.

The application relates to the field of soil improvement, and specifically discloses a soil fumigant for preventing and treating soil-borne diseases and application. The soil fumigant is a sulfuryl fluoride compound, and one fluorine atom in the sulfuryl fluoride structure is replaced by a short-chain alkyl group, a short-chain alkenyl group or a halogenated short-chain alkyl group. The soil fumigant has a broad-spectrum effect on the prevention and treatment of soil-borne pathogenic fungi, bacteria, pythium and root-knot nematodes, is a high-value crop soil-borne disease prevention and control product, and solves the problem of shortage of existing soil fumigants.

The present application provides a kind of plant pathogenic soil biological control agent and its preparation method and application technology, which is composed of multiple different genera of biocontrol strains and their metabolite concentrated solid 70-90% and auxiliary materials 10-30% with complementary mechanism and target.The fermentation concentrated liquid of multiple strains is mixed with carrier, and then spray dried to obtain the compound bacteria fermentation liquid concentrated solid;Then it is mixed with auxiliary materials to granulate to obtain the present application.The present application is used for the prevention and control of plant diseases caused by one or several of fusarium, pythium, pathogenic streptomyces, rhizoctonia solani, raoultella, erwinia carotovora and other plant pathogenic bacteria and nematodes that can survive in soil and its pathogenic soil.The present application effectively improves the microecological environment of pathogenic soil, induces plant disease resistance effect, constructs a good plant soil ecosystem and promotes plant growth and development.

This invention relates to high-protein-producing fungal strains belonging to the genera *Trichoderma*, *Aspergillus*, *Fusarium*, *Penicillium*, *Basilaria*, *Aureospora*, or *Pythium*, preferably *Trichoderma*, wherein the expression of at least one protein encoded by a gene selected from ID 65290, ID 120583, ID 43599, ID 35768, ID 80200, and ID 122457 or its ortholog is modified. The invention also relates to various uses of this strain and methods of genetic modification.