207 results about "Pythium" patented technology

The invention discloses a gene engineering bacterial strain used for producing phenazine-1-methanamide and its purpose; which is characterized in that knockout of psrA or rpeA gene in Pseudomonas chlororaphis HT66(Pseudomonas chlororaphis HT66) CCTCC NO: M 2013467 genome is carried out. According to the invention, a strain of Pseudomonas chlororaphis is performed with seed culture, fermentation, extraction, concentration and crystallization to obtain phenazine-1-methanamide with purity of more than 96%. After the deletion of psrA and rpeA gene in the bacterium genome, the fermentation output of phenazine-1-methanamide can reach as high as 1800ppm. The agar plate bacteriostasis tests found that phenazine-1-methanamide has obvious inhibition effect on common plant pathogenic fungi such as Rhizoctonia solani, fusarium oxysporum, pythium wltmum and Stevia Septoria, has good stability and activity, and has good application prospect.

The invention relates to a high-efficiency Trichoderma harzianum strain for controlling plant fungus diseases and promoting plant growth. The Trichoderma harzianum strain has been collected at the China General Microbiological Culture Collection Center, and the collection number is CGMCC No.7230. The Trichoderma harzianum strain provided by the invention has the advantages of high capacity for inhibiting plant pathogenic fungus growth, no environment pollution and high ecological safety, can be widely used for controlling plant pathogenic fungi, especially Pythium spp., Fusarium spp. and Botrytis cinerea, and has high application value in biological control on melons, fruits, vegetables and crops. The high-efficiency Trichoderma harzianum strain can be used independently, or used together with other organic and inorganic nutrients required by plant growth; and the prepared biological compound fertilizer has obvious effects on promoting growth, inhibiting premature senility, preventing diseases, increasing yield and improving quality.

Strains of Rhizobium leguminosarum biovar viceae have antifungal activity against the pathogen Pythium ultimum. Compositions and methods for treating or protecting plants susceptible to Pythium ultimum damage, and Pythium sp. “group G” damage in particular, are provided. Such strains include, for example, the strains deposited in the International Depository Authority of Canada under accession numbers IDAC 200704-01, IDAC 200704-02, IDAC 200704-03, and IDAC 200704-04.

The invention provides pseudomonas chlororaphis for preventing and treating the crop fusarium disease and applications thereof. The preservation number of the pseudomonas chlororaphis is CGMCC No. 7729. The Pcho01 bacterium strain of the pseudomonas chlororaphis has a high effective antagonistic action, is capable of being applied to the preventing and treating wheat scab caused by fusarium, and the preventing and treating efficiency in the field is maintained above 60%. The Pcho01 bacterium strain also has a very good antagonistic action on botrytis cinerea, rice sheath blight fungus, bipolaris maydis, pythium wltmum, rhizopus, and pseudomonas solanacearum, and can be used to prevent and treat the diseases caused by those bacteria mentioned above.

The invention relates to a sterilization composition containing cyanoacrylate bactericide and metalaxyl-M. The sterilization composition is prepared by carrying out binary compounding on effective components, namely the cyanoacrylate bactericide and the metalaxyl-M. The mass ratio of the cyanoacrylate bactericide to penconazole is 1-40 to 40-1; the mass percentage of the effective components, namely the cyanoacrylate bactericide and the metalaxyl-M, in the preparation is 1-80 percent; and other components are pesticidally allowable and acceptable auxiliary components. The sterilization composition can be in the form of missible oil, suspension agent, wettable powder, water diffusion particle, water emulsion, microemulsion, granules and micro capsules and can be used for preventing and treating downy mildew, phytophthora root rot, pythium, wheat gibberellic disease, cotton fusarium wilt, banana panama disease, fusarium moniliforme sheld, watermelon fusarium wilt and the like.

The invention discloses a biological bacterium prevention compound agent used for preventing and curing a soil-borne disease of the crops, which is characterized in that the contents and weight ratios of all components are: 7 to 9 of dried grass fungus gemma bacillus fermentation broth, 7 to 9 of green wood mold sporule powder, 60 to 80 of diatomaceous earth or light calcium carbonate, 1 to 3 of potassium acid phosphate and 2 to 5 of sodium lignin sulfonate. Through the ferment cultivating to the dried grass fungus gemma bacillus, the fermentation broth is obtained, and the fermentation broth with certain bacterium content is compounded with the green wood mold sporule powder, a microorganism compound agricultural drug is made based on a carrier of diatomaceous earth and by adding suitable nourishing elements. The dried grass fungus gemma bacillus and the green wood mold sporule powder form an effect of mutual predominance compensation. The invention is used for preventing and curing soil-borne diseases, such as a damping off disease, a stand drying disease, a root corrosion disease and a blast which are caused by corrupting leaf mold and fusarium. The product is harmless and is steady and durable in drug effect.

Cyazofamid belongs to sulfanilamide imidazole bactericide and has high bioactivity on oomycete fungi such as phytophthora capsici, downy mildew, pseudo downy mildew and pythium and brassica plasmodiophora brassicae of plasmodiophoromycetes. A preparation method for cyazofamid aqueous emulsion has a formulation comprising 1 to 30 percent of the cyazofamid, 2 to 15 percent of emulsifying agent, 0.2 to 5 percent of co-emulsifying agent, 10 to 20 percent of solvent, 10 to 20 percent of latent solvent, 0.1 to 5 percent of anti-freezing agent, 2 to 10 percent of stabilizing agent, 0.1 to 2 percent of defoaming agent, 0.1 to 5 percent of thickening agent, 0.1 to 4 percent of pH value conditioner and the balance of deionized water, and comprises the following steps: adding the latent solvent into the deionized water according to a ratio; dispersing the latent solvent in a high-shear preparation kettle and stirring the solution evenly; adding cyazofamid raw powder and solvent as well as latent solvent mother solution into the solution; shearing the mixed solution at high speed; and finally adding the thickening agent into the mixed and stirring the resulting solution evenly to obtain the cyazofamid aqueous emulsion. The cyazofamid aqueous emulsion is a new environment-friendly water-based dosage form of agricultural bactericide, uses water as a dispersive medium to replace an expensive organic solvent, reduces environment and dust pollution, and is a new dosage form with development prospect.

The invention discloses a compound biological preservative. The compound biological preservative comprises the components as follows: 0.1-5 g of component B is added in each 1L of component A, the component A is fermentation liquor of Bacillusamyloliquefacienssubsp.plantarum BGP20 strains, and the component B is natamycin. The compound biological preservative is used for preventing and controlling diseases of fruits and vegetables in a storage period and particularly can be used for simultaneously preventing and treating bacterial soft rot and various fungal diseases (for example, phytophthora capsici, rhizopus soft rot, pythium blight and the like) of fruits and vegetables in the storage period, avoiding or reducing residual chemical pesticides and agricultural antibiotics and maintaining the quality of the fruits and vegetables in the storage period, so that the compound biological preservative has good economic and social benefits.

The invention relates to Bacillus amyloliquefaciens LX-J1 and application thereof. A preparation of the Bacillus amyloliquefaciens LX-J1 is prepared from a fermentation solution, copper sulfate, a phosphorus compound, a potassium compound and xanthan gum. Compared with the prior art, the liquid preparation of Bacillus amyloliquefaciens LX-J1 disclosed by the invention has the advantages that the inhibition ratio for rhizoctonia cerealis is up to 87.5%, the inhibition ratio for rhizoctonia solani is up to 78.82%, the inhibition ratio for pythium ultinum trow is up to 87%, the inhibition ratio for sclerotium rolfsii is up to 80.67%, the inhibition ratio for potato black scurf is up to 78.57%, and the inhibition ratio for corticium rolfsii saccardo can be up to more than 83%.

The invention provides a bacterial biological fungicide and a preparation method thereof. Microbial pesticide living Pseudomonas putida is used as a starting strain; and the bacterial biological fungicide is obtained after fermentation and reproduction. The bacterial biological fungicide can be used for nearly all crops infected by Pythium, Rhizoctonia and Fusarium, is safe for the crops, and has good effect on the crops such as cucumber, tomato, scallion, eggplant, green pepper, cotton, watermelon, sesame and the like. The bacterial biological fungicide has the greatest advantages that the bacterial biological fungicide is all nature, harmless, safe to people and livestock and environment-friendly; the pathogenic bacteria are not easy to produce resistance; and the bacterial biological fungicide is favorable for production of green vegetables, and has huge economic, ecological and social benefits.

The invention relates to a botanical essential oil for the control of plant pathogenic fungus and a preparation method thereof. The essential oil of the invention is extracted from star aniseseen fruits and contains 29 main ingredients through gas chromatography-mass spectrometry analysis, wherein, trans-anethole accounts for 83.483 percent. The invention first confirms that the trans-anethole is the main antibacterial active ingredient in the essential oil from the star aniseseen as well as the essential oil from the star aniseseen and the trans-anethole thereof has significant inhibition activity for a plurality of plant pathogenic fungus such as early blight of tomato, stem end rot of mango, corn leaf blight, wheat scab, cucumber fusarium wilt, tomato fusarium wilt, cotton fusarium wilt, rice blast fungus, pythium aphani-dermatum, wheat sheath blight, rice sheath blight, etc. and can control plant diseases.

The invention provides a crop Pythium biological control bacillus subtilis strain and belongs to the biotechnological sphere. The invention relates to a crop Pythium bacillus subtilis strain and its biological crop preserved number is CGMCC1732. The said strain can reduce the growth of Ralstonia solanacearum and the biological control experiment indicates that it can prevent and cure the Granville wilt. Simultaneously the invention also provides an antagonistic protein blend obtained from the said strain and the blend has higher application value in crop Pythium biological control.

A method of improving the growth of plants belonging to the family Cannabaceae sensu stricto by providing a plant nutrient solution about the plant roots and a gas mixture circulating about the plant shoot; by selecting the gas mixture and plant nutrient solution temperature independently of the other; and providing a plant nutrient solution to gas mixture temperature differential of approximately 0° F. or of at least approximately 15° F. during different phases of plant development, in order to change the plant development to improve a desired plant organ for industrial, scientific, and medical purposes. Infection of plants by Pythium, Fusarium, Verticillium, and Rizoctonia are treated or prevented by selecting a gas mixture temperature that is approximately 65 to 85° F. and a plant nutrient solution temperature that is approximately 35 to 65° F.

The invention relates to a method for preventing and controlling ginger blast, and mainly solves the problems that the existing preventing and controlling technology pollutes and damages the soil structure. The method is characterized in that: I, adopting the biological bacteria of the following amount: (1) 500-900 g of primary bacillus subtilis per mu, (2) 10-30 g of pythium oligadrum per mu, and (3) 1000-2000 g of flora consisting of phosphorus-dissolving bacteria, potassium dissolving bacteria, actinomycetes, antibiotic bacteria, nitrobacteria, photosynthetic bacteria, aspergillus, streptomyces and live beneficial fungi; II, adding 500 kg of fermentation soybean cake or 4 square meters of fowl manure into (3) of I, and fermenting for 8-10 days; III, before planting ginger, mixing (1) and (2) of I proportionally, and irrigating the plot; IV, after planting ginger, mixing (1) and (2) of I proportionally, and irrigating the plot at an interval of 20 days; and V, when seeding ginger and topdressing, uniformly scattering the fertilizer made by II. The method provided by the invention is scientific and reasonable, effectively improves soil and has obvious effect of above 90% in preventing and controlling ginger blast; and moreover, the method has certain effect in preventing and controlling ginger rhizome rot, and reduces the cost by **** in comparison with the prior art.

Disclosed is the agricultural pesticide containing camptothecine or camptothecine derivatives and the use thereof, wherein the pesticide comprises camptothecine or its derivatives 0.02-2.0%, emulsifying agent 1.0-20%,penetrating agent 0.5-10%, and balancing organic solvent. The pesticide can be used for controlling agronomic crop downy mildew, leaf streak, pythium blight, head blight, root and stem rot, rhizoctonia rot, damping-off, wilt,bitter rot, powdery mildew, black shank, brown spot, leaf scab, ring rot, black pox, gray mold,phytophthora rot, ulcer disease, black spot, bacterial wilt, angular leaf spot, leaf streak and leaf scar.

The invention provides a pesticide composition capable of preventing and controlling plant diseases and particularly relates to a pesticide active composition which consists of amisulbrom or crystals thereof and one or more of tebuconazole, fenbuconazole, metconazole, tetraconazole, triticonazole, bromuconazole, fluquinconazole and myclobutanil in a compounding way, wherein the mass ratio of amisulbrom to other active substances is 1:9 to 9:1 and the weight percentage of effective components is 1-90 percent. The pesticide composition can prevent and control virus diseases, downy mildew, gray mold, droop, anthracnose, epidemic diseases, blight, pythium rot, verticillium wilt, stalk break, powdery mildew, rust diseases and gibberellic diseases.

The invention relates to a rice planting method. The method includes the following steps that firstly, seed soaking is conducted, wherein empty grains, damaged seeds and heterogeneous seeds in rice seeds are removed; secondly, seedling raising is conducted, wherein a rice seedling bed which is high in terrain, flat, moderate in water seepage and convenient to drain and irrigate serves as a rice seedling raising field, pig manure and nitrogen-phosphorus-potassium composite fertilizer are applied to each mu of the rice seedling bed, then the seeds are sown evenly, and the rice seedling bed is covered with a plastic film; thirdly, transplanting is conducted, wherein during soil preparation before rice transplanting, compost farmyard manure and urea are applied to each mu of soil; fourthly, topdressing is conducted; fifthly, field management is conducted, wherein when seedlings grow, weeds need to be pulled out, pesticides are used for eliminating pests, the shallow water management is mainly conducted in the tillering stage, germination and growth of low position tillering are promoted, and field drying is conducted at proper time; sixthly, disease and pest prevention is conducted, wherein people need to pay attention to rice damping-off, pythium rot and the rice planthopper disease of the rice seedling bed, and pesticides are used for prevention if necessary; seventhly, harvesting is carried out. The planting method enhances rice growth in different stages and further remarkably increases the yield of rice.

The invention discloses an aryl triazole compound containing chlorinated cyclopropane and a preparation method and application thereof. The structural formula of the aryl triazole compound containing chlorinated cyclopropane is shown in formula I, wherein X represents C=O or CHOH, and R represents a substituent phenyl group; the substituent in the substituent phenyl group is any one of the following groups: halogen, methyl group, methoxyl group, nitro group, trifluoromethyl group, amino group, and acetyl amino group. The preparation method, provided by the invention, adopts cheap raw materials, and takes a simple reaction route; the synthesized compound has both plant growth regulating activity and bactericidal activity, thereby having a wider application range; in application experiments of the aryl triazole compound containing chlorinated cyclopropane, the plant growth regulating activity shows that the synthesized compound manifests excellent plant growth regulating activity; in application experiments of the aryl triazole compound containing chlorinated cyclopropane, the bactericidal activity test result shows that the compound has excellent growth inhibition effect on sclerotinia sclerotiorum, tomato fungus phytophthora, botrytis cinerea, rhizoctonia solani seedbed, blast fungus, asparagus stem blight, fusarium graminearum, pythium germs, and the like.

The invention relates to a microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof, which belong to the field of microbial pesticide. The microbial preparation is prepared by a conventional method. A production strain of the microbial preparation is actinomyces Da07210 which is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the actinomyces Da07210 is CGMCC No.2561. The strain is prepared into a liquid preparation through liquid fermentation, or is prepared into wettable powder by mixing the strain with an adsorption carrier according to a certain proportion, filtering, concentrating, grinding and airing the mixture. The microbial preparation has the advantages that the microbial preparation can prevent and control banana wilt, plague, seedling blight, damping-off and the like caused by fusarium, phytophthora, rhizoctonia and pythium pathogenic fungi, and can also stimulate root systems of plants to grow and promote the growth of plant seedlings.

D-aminoacylase derived from fungi is provided. The fungi capable of producing D-aminoacylase include those belonging to the genus Hypomyces, Fusarium, Auricularia, Pythium, and Menisporopsis. The fungal D-aminoacylase is useful for efficiently producing D-amino acids from N-acetyl-D-amino acids.

The invention discloses a germicide composition. The germicide composition comprises a synergic effective dose of cyprodinil and one of cyazofamid, cymoxanil, metalaxyl and hymexazol, wherein the weight part ratio of two active ingredients in the germicide composition is 40-1:1-40. The germicide composition at least comprises one surfactant which contributes to dispersing the active ingredients in water during application. The germicide composition has protection activity, treatment activity and eradicating activity, can be used for various crops and has extremely high bioactivity on oomycetes fungi such as phytophthora capsici, peronospora parasitica, pseudoperonospora parasitica, pythium and plasmodiophoromycetes plasmodiophora brassicae woron.

The invention discloses a lawn special slow/controlled-release fertilizer and a preparation method thereof. The lawn special slow/controlled-release fertilizer is prepared from the following components in percentage by mass: 25 to 30 parts of nitrogen fertilizer, 8 to 12 parts of phosphate fertilizer, 12 to 16 parts of potash fertilizer, 25 to 40 parts of fermented earthworm feces, 2 to 5 parts of humic acid, 3 to 6 parts of compound amino acid, 2 to 5 parts of medium trace element, 1 to 2 parts of bactericide, and 1 to 3 parts of coating agent. The lawn special slow/controlled-release fertilizer is prepared by the following steps of mixing the nitrogen fertilizer, the phosphate fertilizer, the potash fertilizer, the fermented earthworm feces, the humic acid, the compound amino acid, the medium trace element and the bactericide, granulating, smearing the coating agent to fertilizer particles by a drum coating technique, and performing processes such as drying, sieving, and detecting. Compared with the prior art, the lawn special slow/controlled-release fertilizer has the advantages that the effective period is long, the nutrient requirement of a whole growth cycle can be met by one-time fertilizer applying, the loss of the fertilizer is decreased, and the utilization rate of the fertilizer is improved; by adding the bactericide, the pythium blight can be effectively prevented and controlled, and the used labor of management and curing is decreased.

The invention relates to a sterilization composition containing pyraclostrobin and efficient metalaxyl-M. The sterilization composition comprises effective components including the pyraclostrobin and the efficient metalaxyl-M, which are compounded in a binary manner, wherein the mass ratio of the pyraclostrobin to penconazole is (1 to 40): (40 to 1); the mass percent of the effective components including the pyraclostrobin and the efficient metalaxyl-M in a preparation is 1 percent to 80 percent, and the balance is auxiliary components allowable and acceptable in pesticides. The sterilization composition provided by the invention has dosage forms including missible oil, a suspending agent, wettable powder, water dispersible granules, emulsion in water, micro-emulsion, granules and microcapsules. The sterilization composition can be used for effectively preventing and treating downy mildew, phytophthora capsici, pythium disease, wheat scab, fusarium wilt of cotton, panama disease of bananas, bakanae disease of rice, watermelon fusarium wilt and the like.

The invention relates to a polygonum aviculare botanical bactericide and a preparation method thereof, belonging to the technical field of pesticides. The invention aims at disclosing a compound botanical bactericide formula and a preparation method thereof. The polygonum aviculare botanical bactericide is mainly prepared from polygonum aviculare, radix scutellariae, black nightshade herb, radix gentianae, rhizoma atractylodis and the like. The preparation method comprises the steps of 1, drying and crushing the raw materials, then sieving and mixing the raw materials; 2, performing ultrasonic treatment on the mixture after being subjected to cold soak in ethanol, filtering to obtain a filtered liquid, evaporating and condensing until the filtered liquid becomes paste. The polygonum aviculare botanical bactericide has a relatively good bacteriostasis function on multiple pathogenic fungal plant pathogens such as rhizoctonia solani, pythium blight pathogen, fusarium graminearum, alternaria solani and carbon-color helminthosporium carposaprum, has the effects obviously better than the chemical disinfectant thiram. The raw materials adopted in the invention are purely natural plants, so that the preparation cost is low, and small-size industrial production can be carried out; furthermore, as the plants are Chinese herbal medicines, the polygonum aviculare botanical bactericide can be degraded rapidly after the use, is safe for people and live stock, good in environmental compatibility and free from secondary pollution to the environment, and meets the current concept of green food and environment sustainable development strategy.

The invention discloses a special sustained and controlled release fertilizer for flowers, and a preparation method thereof. The fertilizer comprises, by mass, 25-30 parts of a nitrogen fertilizer, 8-12 parts of a phosphorus fertilizer, 12-16 parts of a potassium fertilizer, 25-40 parts of fermented ground beetle dung, 2-5 parts of humic acid, 3-6 parts of composite amino acids, 2-5 parts of medium and trace elements, 1-2 parts of a bactericide and 1-3 parts of a coating agent. The preparation method comprises the following steps: mixing and granulating the nitrogen fertilizer, the phosphorus fertilizer, the potassium fertilizer, the fermented ground beetle dung, humic acid, the composite amino acids, medium and trace elements and the bactericide, smearing the coating agent on fertilizer granules through a fluidized bed coating technology, drying the coated granules, screening the dried granules, and detecting the screened granules to prepare the special sustained and controlled release fertilizer for flowers. Compared with fertilizers in the prior art, the fertilizer disclosed in the invention has the advantages of long effective period, meeting of nutrient demands of the whole growth period through one-shot fertilization, reduction of the fertilizer loss, fertilizer utilization rate improvement, realization of effective prevention and control of Pythium diseases through adding the bactericide, and reduction of the management and maintenance labor.

The invention provides a microbial agent. The microbial agent comprises the following components in parts by weight: 20 to 60 parts of hydrogen-oxidizing bacteria, 20 to 60 parts of nitrogen-fixing bacteria, 30 to 50 parts of actinomycetes and 30 to 60 parts of fungi, wherein the actinomycetes can be actinoplanes, streptomycetes or nocardia; and the fungi can be pythium or trichoderma. The microbial agent disclosed by the invention generates a certain synergistic effect through the combination of the hydrogen-oxidizing bacteria, the nitrogen-fixing bacteria, the actinomycetes and the fungi, and can improve the yield of wheat, promote the development of wheat root systems and improve the good earth holding capacity of roots for soil, so that main roots, side roots and fibrous roots are deeper and wider in coverage and are difficult to lodge and stronger in water absorption, eustipes close to the ground has better toughness and strength, the toughness and strength of the stems can be improved, and the stems are difficult to break. The microbial agent provided by the invention can shorten the growth and maturation time, enable crops to be matured in advance, reduce the risks of vigorous growth of seedlings and green remaining and late maturation of plants, and promote early fruiting and maturation of the plants to appear in the market.