11 results about "Pythium" patented technology

The application discloses a specific detection target Pc_s103050 of a pathogenic bacterium of cedar root rot and application, and the specific detection target comprises a nucleotide sequence as shown in SEQ ID NO. 13, i.e. Pc_s103050. The LAMP primer composition designed based on the target gene disclosed by the application can be used for rapidly and efficiently detecting Pythium cedri, and the detection method has the advantages of high specificity, high accuracy, good sensitivity, simple operation and naked-eye observation of results, and Pythium cedri can be identified when the DNA reaches 100 pg mu L ‑1 .

The present application belongs to the technical field of tobacco planting, and particularly relates to a method for targeted removal of pathogenic bacteria in continuous tobacco field, which comprises the following steps: S1, land preparation: removing field residues and weeds before transplanting flue-cured tobacco seedlings; S2, pesticide application: applying base fertilizer and removal agent to the tobacco ridge area of the tobacco field; S3, ridging: loosening and ridging the soil where the removal agent is applied; S4, film mulching and sealing: mulching after ridging, and sealing the tobacco ridge; S5, film breaking and air release: breaking the film at the interval of the tobacco seedling nest distance and releasing the air in the film; S6, trial planting and verification: planting 3-5 tobacco seedlings or vegetable seedlings per mu, watering and observing, and then transplanting after no abnormality of the plants; S7, transplanting tobacco seedlings; in use, the inhibition rates of Fusarium, Pseudomonas and Pythium in the continuous tobacco field are all above 86%, the comprehensive removal rate of pathogenic bacteria is greater than or equal to 86%, the incidence of tobacco seedlings is reduced from 36.8% to less than 7%, the frequent occurrence of soil-borne diseases is solved from the source, the bacteria are targetedly inhibited, no fallow is needed, and the cost is low.

The application relates to the field of soil improvement, and specifically discloses a soil fumigant for preventing and treating soil-borne diseases and application. The soil fumigant is a sulfuryl fluoride compound, and one fluorine atom in the sulfuryl fluoride structure is replaced by a short-chain alkyl group, a short-chain alkenyl group or a halogenated short-chain alkyl group. The soil fumigant has a broad-spectrum effect on the prevention and treatment of soil-borne pathogenic fungi, bacteria, pythium and root-knot nematodes, is a high-value crop soil-borne disease prevention and control product, and solves the problem of shortage of existing soil fumigants.

This invention relates to high-protein-producing fungal strains belonging to the genera *Trichoderma*, *Aspergillus*, *Fusarium*, *Penicillium*, *Basilaria*, *Aureospora*, or *Pythium*, preferably *Trichoderma*, wherein the expression of at least one protein encoded by a gene selected from ID 65290, ID 120583, ID 43599, ID 35768, ID 80200, and ID 122457 or its ortholog is modified. The invention also relates to various uses of this strain and methods of genetic modification.

The present application relates to the application of spermine and / or spermidine in preventing and treating plant diseases caused by Phytophthora. The present application finds that spermine and spermidine can effectively inhibit the spore germination and mycelium growth of Phytophthora capsici Leonian and P. infestans Mont. and can effectively prevent and treat the plant diseases caused by P. capsici Leonian and P. infestans Mont., including pepper Phytophthora blight, tomato Phytophthora blight, potato late blight and tomato late blight, and significantly reduce the plant disease area.

This invention discloses a specific detection target Pc_s460833 for the cedar pathogen Pythium cedri and its application. The specific detection target includes the nucleotide sequence Pc_s460833 as shown in SEQ ID NO.7. The LAMP primer composition designed based on the above target gene disclosed in this invention can be used for rapid and efficient detection of Pythium cedri. The detection method has the advantages of high specificity, high accuracy, good sensitivity, simple operation, and visually observable results. When the DNA reaches 100 pg·μL... ‑1 It can identify Pythium cedarum in a short time, making it highly practical.

This invention discloses a method for post-harvest preservation of litchi and prevention of downy mildew. This invention is the first to discover the effect of 10-undecenoic acid on the fungus causing litchi downy mildew (… Peronophythora litchii 10-Undecenoic acid (10-Undecenoic acid) has an inhibitory effect; treatment of litchi fruit with 10-Undecenoic acid can significantly reduce the incidence and disease index of the disease, delay fruit decay, maintain storage quality, and achieve the prevention and control of litchi downy mildew and post-harvest preservation. This invention provides a safe and efficient solution for the prevention and control of litchi downy mildew and post-harvest preservation, and has good application prospects.

This invention discloses an arginine methyltransferase protein from *Phytophthora* plant pathogenic oomycetes, its encoding gene, and its applications. The arginine methyltransferase protein provided by this invention is shown in sequences 6-8; its encoding gene is shown in sequences 1-3. Experiments have demonstrated that the protein of this invention plays an important role in the growth, development, and pathogenicity of *Phytophthora capsici*, a typical plant pathogenic oomycete. The absence or reduced expression level of this protein leads to slower mycelial growth rate, reduced sporangium production, decreased zoospore numbers, and reduced pathogenicity of *Phytophthora capsici*. Corresponding arginine methyltransferase inhibitors exhibit varying degrees of activity against typical plant pathogenic oomycetes such as *Phytophthora capsici*, *Phytophthora soybeani*, and *Phytophthora pathogenica*. The gene provided by this invention has significant application potential in the prevention and control of crop blight caused by *Phytophthora* plant pathogenic oomycetes. Novel fungicides developed based on this protein as a target have important practical significance for controlling the occurrence and spread of crop blight caused by *Phytophthora*.

This invention discloses a specific detection target Pc_s376271 for Pythium cedri and its application. The specific detection target includes the nucleotide sequence Pc_s376271 as shown in SEQ ID NO.27. The LAMP primer composition designed based on the above target gene disclosed in this invention can be used for rapid and efficient detection of Pythium cedri. The detection method has the advantages of high specificity, high accuracy, good sensitivity, simple operation, and visually observable results. When the DNA concentration reaches 100 pg·μL... ‑1 It can identify Pythium cedarum in a short time, making it highly practical.

The application discloses genes GmPMI1 and GmPMI1R for improving plant resistance to soybean blight and application thereof. The nucleotide sequences of the genes GmPMI1 and GmPMI1R are shown in SEQ ID NO. 1 or SEQ ID NO. 3 respectively. The protein encoded by the genes GmPMI1 and GmPMI1R in the application exerts an anti-disease effect by being secreted to the extracellular, thereby enhancing the plant disease resistance, especially the disease resistance to the Pythium, and the plant disease resistance to the Pythium can be significantly enhanced. The application can be applied to crop breeding and disease resistance improvement, and is expected to improve the plant disease resistance to the blight, and further promote the reduction of pesticide and fertilizer application and the increase of efficiency.

Bacilli and combinations of Bacilli capable of germinating and growing under low temperature conditions; of associating with plant roots; of enhancing plant health, growth, and yield; and of protecting plants against phytopathogenic pests, such as Botrytis, Fusarium, Phytophthora and Pythium.