Application of glycoalkaloids as pesticides for controlling plant diseases

A plant disease and alkaloid technology, applied in the field of plant fungal disease pesticides, can solve problems such as environmental pollution, ecological runaway, chemical pesticide pollution, etc., achieve obvious preventive and therapeutic effects, and prevent infection.

Inactive Publication Date: 2007-09-12
NORTHEAST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term large-scale use of chemical pesticides has caused environmental pollution and ecological loss of control. At the same time, chemical residues have long-term effects on hu

Method used

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  • Application of glycoalkaloids as pesticides for controlling plant diseases
  • Application of glycoalkaloids as pesticides for controlling plant diseases
  • Application of glycoalkaloids as pesticides for controlling plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Research on the inhibitory effect of glycoalkaloids of the present invention on purple spot fungus (Alternaria dauci.f.porri)

[0027] The inhibitory activity of five glycoalkaloids against P. spp. was determined by mycelial growth inhibition method. After the sample was completely dissolved with 1ml of 0.1M HCL, a certain amount of distilled water was added to make the sample mother solution. When in use, add a certain concentration of medicinal liquid into the hot medium, mix evenly and sterilize under high pressure, cool to 55°C and pour it into a 5cm petri dish to cool to make a certain concentration of medicinal medium. Under aseptic conditions, use a puncher with a diameter of 5 mm to cut the bacterium cake from the edge of the test bacteria colony that was previously cultivated at 25 ° C for 5 days on the PDA plate, and use the inoculation needle to transplant the bacterium cake to the drug-carrying medium plate (control) ) center, each concentrati...

Embodiment 2

[0029] Embodiment 2: Research on the inhibitory effect of glycoalkaloids of the present invention on cabbage white spot pathogen (cercosporella albo-maculans)

[0030] The inhibitory activity of five glycoalkaloids against white spot pathogen of cabbage was determined by spore germination method. After the bacteria were cultured on a PDA plate at 25°C for 7 days, the spores were washed with sterile water, filtered, and the spore suspension was diluted to 1×10 6 pcs / ml for use. After the five compounds were dissolved in 0.1M HCL, they were diluted to different concentration gradients of 20ug / g-200ug / g, and the bacterial solution and spore suspension were mixed in equal volumes, and 40ul of the mixed solution was taken and placed on a concave glass slide, repeated 3 times, 25 Cultivate in the dark at ℃, investigate the spore germination rate of each treatment when the blank control spores have germinated above 90%, and calculate the bacteriostatic rate and EC 50 value.

[003...

Embodiment 3

[0033] Example 3: 1% WP

[0034] Mix 1 part of any compound of 1-5 of the present invention with 10 parts of WPA-9503 compounding aid and 89 parts of diatomaceous earth by weight, and grind to obtain 100 parts of wettable powder.

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Abstract

The present invention relates to farm chemical with glucoside alkaloid as the effective component for preventing and controlling plant mycosis and its application. The fungicidal glucoside alkaloid preparation has up to five kinds of glucoside alkaloid with obvious effect of preventing and controlling plant diseases caused by Verticillium, monospermous rust fungi, Fusarium and other fungi. The fungicidal glucoside alkaloid preparation has broad spectrum, low toxicity, environment friendship, low cost and other features.

Description

technical field [0001] The invention relates to the application of glycoside alkaloids as active ingredients as pesticides for preventing and controlling plant fungal diseases. Background technique [0002] The pesticides currently used to prevent and control fungal diseases of vegetables, fruits, grain, cotton, oil and other crops are generally chemical agents, which contain toxic substances such as organophosphorus and chlorine. Long-term large-scale use of chemical pesticides has caused environmental pollution and ecological loss of control. At the same time, chemical residues have long-term effects on humans and animals, leading to poisoning. All countries in the world are paying attention to the pollution of chemical pesticides, and many chemical pesticides are banned or restricted. [0003] Now, the pesticide market has a very urgent demand for biopesticide products, and it is very necessary to develop new biopesticides that are safe, efficient, and broad-spectrum wit...

Claims

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Application Information

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IPC IPC(8): A01N43/14A01N43/90A01P3/00
Inventor 周义发赵雪淞台桂花李盛珏孙芳
Owner NORTHEAST NORMAL UNIVERSITY
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