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Yeast strain capable of antagonizing bacillus anthracis and applications thereof

A technology of yeast strains and anthrax bacteria, which is applied in the fields of application, fungicides, fungi, etc., can solve the problems of difficulty in stabilizing the inhibition rate of anthrax bacteria, difficulty in preventing and controlling fungal diseases, and insufficient biological control and control effects

Inactive Publication Date: 2018-07-20
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to the difficulty in the control of fungal diseases and the lack of biological control effects, the control effect of anthracnose biological control rarely reaches or exceeds that of chemical pesticides, and the inhibition rate of anthracnose bacteria is difficult to stabilize at more than 70%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First, the malt is prepared into a malt liquid culture medium according to the conventional microbial malt treatment method, and the sterilization condition is 115° C. for 20 minutes.

[0025] Secondly, the MLB1 strain was cultured according to the conventional liquid seed culture method of microbial fermentation, with sterilized malt medium, the sugar concentration of the seed culture liquid was 3%, and the sugar concentration of the fermenter culture liquid was 5%. After a series of culture in shake flasks and seed tanks, when the number of yeasts per milliliter of culture solution is 1×10 8 For one hour, 2% of the fermenter culture solution was inserted into the fermenter for cultivation, the temperature was controlled at 27°C, the sterile air was kept continuously fed, the aeration ratio was controlled at 1:1.1, and the dissolved oxygen was maintained at 40%. Incubate for 48 hours.

[0026] Finally, the fermented liquid was collected, which was used as the target c...

Embodiment 2

[0028] The difference from Example 1: the number of yeasts in the seed culture liquid reached 1.8×10 8 Connect the fermenter at 1 hour, the sugar concentration of the culture liquid of the fermenter is 7%, and the cultivation time is 72 hours. Determination of antagonistic capacity. The inhibition rate of anthrax bacteria was 80%.

Embodiment 3

[0030] The difference from Example 1: the number of yeasts in the seed culture liquid reached 2.5×10 8 Connect the fermenter at 1 hour, the sugar concentration of the culture liquid of the fermenter is 7%, and the cultivation time is 60 hours. Determination of antagonistic capacity. The inhibition rate of anthrax bacteria was 85%.

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PUM

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Abstract

The invention relates to a yeast strain capable of antagonizing bacillus anthracis and applications thereof, and belongs to the technical field of microbe application. The yeast strain (Saccharomycescerevisiae-MLB1) capable of antagonizing bacillus anthracis is obtained from nature through strain separation and culture. The yeast strain has been saved in China General Microbiological Culture Collection Center, CGMCC in Jan.6th,2014, and the preservation number is No. 8689. The yeast MLB1 strain is cultured in a malt extract medium, the culture product can antagonize bacillus anthracis, and the inhibition rate on fruit anthracnose is more than 75%.

Description

technical field [0001] The invention relates to a yeast strain capable of antagonizing anthrax, belonging to the technical field of microorganism application. Background technique [0002] Plant diseases are the enemy of agricultural production. According to the statistics of the Food and Agriculture Organization of the United Nations (FAO), the economic losses caused by pests in the world are as high as 120 billion US dollars, which is equivalent to half of China's total agricultural output value. The occurrence of plant diseases not only directly affects the income of farmers in our country, but also affects the development of other related industries, especially the agricultural product processing industry. In 1880, the grape planting industry in the Bordeaux region of France was on the verge of bankruptcy due to downy mildew. The most serious thing is that it is not conducive to the long-term and sustainable development of agriculture and its related industries. [000...

Claims

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Application Information

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IPC IPC(8): C12N1/18A01N63/04A01P3/00C12R1/865
CPCA01N63/30C12N1/185C12R2001/865
Inventor 乔敏余泽芬贾东晨
Owner YUNNAN UNIV
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