Cottonseed meal feed and preparation method thereof

A technology of cottonseed meal and feed, applied in animal feed, animal feed, applications, etc., can solve the problems of heavy workload, harsh process conditions, low degradation rate of gossypol, etc., to increase the content of small molecular peptides and reduce the content of tea saponins , the effect of excellent synergistic performance

Inactive Publication Date: 2015-01-14
RUIAN PULUO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the degradation methods of free gossypol in feed mainly include: physical method and chemical method, but these two methods have the problems of heavy workload, harsh process conditions and low degradation rate of gossypol

Method used

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  • Cottonseed meal feed and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Cottonseed meal pretreatment

[0037] Add 10kg of water to 2kg of cottonseed meal, stir evenly, adjust the pH to 10, and treat at 90°C for 1 hour;

[0038] (2) Bacteria culture

[0039] Cultivation of Aspergillus niger species:

[0040] Sucrose 2.5% (w / v), yeast powder 0.3% (w / v), peptone 1.0% (w / v), K 2 HPO 4 0.1% (w / v), MgSO4 7H 2 O 0.01% (w / v), cultured at 25°C for 2 days.

[0041] Bacillus natto culture:

[0042] Tryptone 1.0% (w / v), yeast extract 0.5% (w / v), glucose 2% (w / v), NaCl 1.0% (w / v), K 2 HPO 4 0.2% (w / v), MgSO 4 ·7H 2 O 0.002% (w / v), cultured at 37°C for 24-36 hours.

[0043] (3) Semi-solid fermentation

[0044] Adjust the pH of the pretreated cottonseed meal in step (1) to 6.5, add 200 g of sugarcane molasses, 100 g of sodium chloride, and 100 g of ammonium sulfate, heat-treat at 90° C. for 1 hour, and inoculate 1.0 L of Aspergillus niger seed solution and Bacillus natto Liquid 3L, fermented for 96h;

[0045] (4) dry crushing

[0046] Th...

Embodiment 2

[0051] (1) Cottonseed meal pretreatment

[0052]Add 5kg of water to 2kg of cottonseed meal, stir evenly, adjust the pH to 9, and treat at 80°C for 2 hours;

[0053] (2) Bacteria culture

[0054] Cultivation of Aspergillus niger species:

[0055] Sucrose 2.5% (w / v), yeast powder 0.3% (w / v), peptone 1.0% (w / v), K 2 HPO 4 0.1% (w / v), MgSO4 7H 2 O 0.01% (w / v), cultured at 25°C for 2 days.

[0056] Bacillus natto culture:

[0057] Tryptone 1.0% (w / v), Yeast Extract 0.5% (w / v), Glucose 2% (w / v), NaCl 1.0% (w / v), K 2 HPO 4 0.2% (w / v), MgSO 4 ·7H 2 O 0.002% (w / v), cultured at 37°C for 12-24 hours.

[0058] (3) Semi-solid fermentation

[0059] Adjust the pH of the pretreated cottonseed meal in step (1) to 7.0, add 100 g of sugarcane molasses, 70 g of sodium chloride, and 50 g of ammonium sulfate, heat-treat at 90°C for 1 hour, and inoculate 1 L of Aspergillus niger seed solution and Bacillus natto seed solution 2L, ferment for 72h;

[0060] (4) dry crushing

[0061] The d...

Embodiment 3

[0067] (1) Cottonseed meal pretreatment

[0068] Add 5kg of water to 2kg of cottonseed meal, stir evenly, adjust the pH to 11, and treat at 80°C for 1 hour;

[0069] (2) Bacteria culture

[0070] Cultivation of Aspergillus niger species:

[0071] Sucrose 2.5% (w / v), yeast powder 0.3% (w / v), peptone 1.0% (w / v), K 2 HPO 4 0.1% (w / v), MgSO4 7H 2 O 0.01% (w / v), cultured at 25°C for 2 days.

[0072] Bacillus natto culture:

[0073] Tryptone 1.0% (w / v), yeast extract 0.5% (w / v), glucose 2% (w / v), NaCl 1.0% (w / v), K 2 HPO 4 0.2% (w / v), MgSO 4 ·7H 2 O 0.002% (w / v), cultured at 37°C for 24-36 hours.

[0074] (3) Semi-solid fermentation

[0075] Adjust the pH of the pretreated cottonseed meal in step (1) to 7.0, add 200 g of sugarcane molasses, 100 g of sodium chloride, and 100 g of ammonium sulfate, and inoculate 600 ml of Aspergillus niger seed liquid and Bacillus natto seed liquid after heat treatment at 80°C for 1 hour. 2.5L, fermented for 48h;

[0076] (4) dry crushing...

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Abstract

The invention discloses a cottonseed meal feed and a preparation method thereof. According to the cottonseed meal feed, the content of gossypol is less than or equal to 100mg / kg. The preparation method comprises the following steps: adding water 2-6 times of the weight of cottonseed meal to the cotton seed meal, uniformly stirring and heating for 1-3 hours; respectively culturing an aspergillus niger strain and a bacillus natto strain; adjusting the pH of the cottonseed meal subjected to pretreatment to 6.5-7.5, adding cane molasses, carrying out heat treatment at 60-90 DEG C for 1 hour, then inoculating the strains, and fermenting for 48-96 hours; and drying and crushing the fermented product to obtain detoxified cottonseed meal. The content of gossypol in the cottonseed meal obtained by final fermentation is less than or equal to 80mg / kg, the small molecule peptide and crude protein content is greatly increased and the cottonseed meal feed can completely used as an animal feed.

Description

technical field [0001] The invention relates to the field of agricultural feed, in particular to a cottonseed meal feed and a preparation method thereof. Background technique [0002] my country is a large cotton-producing country with abundant cotton resources. The annual output of cottonseed can reach nearly 10 million tons, except for a small part reserved for seeding, and the rest is used for oil extraction. The annual production of cottonseed meal reaches hundreds of tons. Among them, the cottonseed meal used for feed only accounts for about 15%, and most of the rest is used as fertilizer. The protein content in cottonseed meal accounts for about 30% to 45%, which is equivalent to soybean meal, and the protein content is 5 to 8 times higher than that of rice and wheat. Cottonseed meal contains 17 kinds of amino acids, of which the content of lysine is low at 1.48%, while the content of arginine is high at 3.6% to 3.8%. The ratio of lysine to arginine far exceeds the ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
Inventor 杨钟华
Owner RUIAN PULUO BIOTECH
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