Streptomyces tubercidicus strain and application thereof
A technology for streptomyces and nodules, applied in the direction of application, fungicides, bacteria, etc., can solve problems such as excessive use of fungicides, achieve good control effects and improve control effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0032] Example 1: Isolation and identification of Streptomyces tubercidicus strain 7-2
[0033] 1. Collection of soil samples
[0034] Three soil samples were collected around the rhizosphere of Taxus chinensis from Jinggangshan, Jiangxi Province. The soil on the surface was removed, and soil samples at a depth of 5-20 cm were collected. After marking, they were brought back to the laboratory to dry naturally.
[0035] 2. Isolation of Actinomycetes
[0036] Separation was performed using the plate dilution method. The air-dried soil sample was ground with a mortar, and 1 g of the sample was weighed and suspended in 9 mL of sterile water, shaken at 40 ° C and 180 rpm for 30 min, and then allowed to stand for 5 min, diluted 10 times in turn, and prepared into 10 -2 , 10 -3 , 10 -4 0.1 mL of each suspension of different concentrations were added to the modified HVA medium (with potassium dichromate with a final concentration of 100-200 ppm) on the plate, evenly coated and the...
Example Embodiment
[0050] Example 2: Antagonism assay of Streptomyces tubercidicus strain 7-2 against pathogenic bacteria
[0051] Using the plate confrontation culture method, the Streptomyces tubercidicus strain 7-2 was tested for antagonism against asparagus stem blight, rice blast, soybean anthracnose and mushroom brown rot. First, streak strain 7-2 on both sides of the edge of the PDA medium. After 3 days, insert the test pathogenic bacteria cake with a diameter of 5 mm in the center of the plate, and culture it at 28 °C for 5 days to measure the antibacterial effect of strain 7-2 on the test pathogenic bacteria. Taking the tested pathogens without antagonistic bacteria as the control, the results show that strain 7-2 has very good antagonistic effects on asparagus stem blight, rice blast, soybean anthracnose and mushroom brown rot ( figure 2 ).
Example Embodiment
[0052] Example 3: Inhibitory effect of Streptomyces tubercidicus (Streptomyces tubercidicus) strain 7-2 fermentation broth on pathogenic bacteria
[0053] The spores of strain 7-2 activated by Gao's No. 1 medium were inserted into the ISP2 liquid medium, the filling volume was 80mL / 250mL conical flask, the inoculum volume was 2-5%, the temperature was 25-28°C, and the rotation speed was 180-200rpm. , the fermentation culture time is 5-7d, and the fermentation broth is obtained, and then filtered with a 22 μm microporous membrane to obtain a sterile fermentation filtrate, which is used for later use.
[0054] Take 1 mL of the sterile fermentation filtrate of strain 7-2 and add it to 9 mL of PDA medium, pour it into a petri dish after mixing, insert the 5 mm diameter test pathogenic bacteria cake into the center of the PDA medium plate, and place it at 28 ° C and cultivate it for 7 days. Measure the colony diameter. Taking no sterile fermentation filtrate as a control, the inhi...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap